Human Gene PRSS21 (ENST00000005995.8) from GENCODE V44
  Description: Homo sapiens serine protease 21 (PRSS21), transcript variant 5, non-coding RNA. (from RefSeq NR_073012)
RefSeq Summary (NM_006799): This gene encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. The encoded protein is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The encoded protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and may be involved in progression of testicular tumors of germ cell origin. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012].
Gencode Transcript: ENST00000005995.8
Gencode Gene: ENSG00000007038.11
Transcript (Including UTRs)
   Position: hg38 chr16:2,817,237-2,821,719 Size: 4,483 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr16:2,817,269-2,821,605 Size: 4,337 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:2,817,237-2,821,719)mRNA (may differ from genome)Protein (314 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TEST_HUMAN
DESCRIPTION: RecName: Full=Testisin; EC=3.4.21.-; AltName: Full=Eosinophil serine protease 1; Short=ESP-1; AltName: Full=Serine protease 21; Flags: Precursor;
FUNCTION: Could regulate proteolytic events associated with testicular germ cell maturation.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor (Potential).
TISSUE SPECIFICITY: Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.
SIMILARITY: Belongs to the peptidase S1 family.
SIMILARITY: Contains 1 peptidase S1 domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: PRSS21
Diseases sorted by gene-association score: testicular cancer (3), dracunculiasis (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • C036990 2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline
  • D014415 Tunicamycin

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 61.94 RPKM in Testis
Total median expression: 74.32 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.3032-0.228 Picture PostScript Text
3' UTR -33.70114-0.296 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009003 - Pept_cys/ser_Trypsin-like
IPR018114 - Peptidase_S1/S6_AS
IPR001254 - Peptidase_S1_S6
IPR001314 - Peptidase_S1A

Pfam Domains:
PF00089 - Trypsin

ModBase Predicted Comparative 3D Structure on Q9Y6M0
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004252 serine-type endopeptidase activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008236 serine-type peptidase activity
GO:0016787 hydrolase activity

Biological Process:
GO:0006508 proteolysis
GO:0007283 spermatogenesis

Cellular Component:
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031225 anchored component of membrane


-  Descriptions from all associated GenBank mRNAs
  AB031331 - Homo sapiens tesp-1 mRNA for eosinophil serine protease 1 splicing variant, complete cds.
JD460702 - Sequence 441726 from Patent EP1572962.
JD138107 - Sequence 119131 from Patent EP1572962.
BC074999 - Homo sapiens protease, serine, 21 (testisin), mRNA (cDNA clone MGC:104150 IMAGE:30915610), complete cds.
BC075000 - Homo sapiens protease, serine, 21 (testisin), transcript variant 1, mRNA (cDNA clone MGC:103990 IMAGE:30915398), complete cds.
AY359034 - Homo sapiens clone DNA42551 PRSS21 (UNQ266) mRNA, complete cds.
AF058300 - Homo sapiens testisin (TEST1) mRNA, complete cds.
AB031329 - Homo sapiens esp-1 mRNA for eosinophil serine protease, complete cds.
AB031330 - Homo sapiens esp-1 mRNA for eosinophil serine protease, complete cds.
KJ898316 - Synthetic construct Homo sapiens clone ccsbBroadEn_07710 PRSS21 gene, encodes complete protein.
LF340689 - JP 2014500723-A/148192: Polycomb-Associated Non-Coding RNAs.
MA576266 - JP 2018138019-A/148192: Polycomb-Associated Non-Coding RNAs.
LF340688 - JP 2014500723-A/148191: Polycomb-Associated Non-Coding RNAs.
MA576265 - JP 2018138019-A/148191: Polycomb-Associated Non-Coding RNAs.
JD541405 - Sequence 522429 from Patent EP1572962.
JD046577 - Sequence 27601 from Patent EP1572962.
JD501306 - Sequence 482330 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Y6M0 (Reactome details) participates in the following event(s):

R-HSA-8940388 GPLD1 hydrolyses GPI-anchors from proteins
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000005995.1, ENST00000005995.2, ENST00000005995.3, ENST00000005995.4, ENST00000005995.5, ENST00000005995.6, ENST00000005995.7, ESP1, NR_073012, Q9NS34, Q9P2V6, Q9Y6M0, TEST1, TEST_HUMAN, uc002crt.1, uc002crt.2, uc002crt.3, uc002crt.4, uc002crt.5, uc002crt.6, UNQ266/PRO303
UCSC ID: ENST00000005995.8
RefSeq Accession: NM_006799
Protein: Q9Y6M0 (aka TEST_HUMAN)
CCDS: CCDS10478.1, CCDS45388.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.