Human Gene MYH4 (ENST00000255381.2) from GENCODE V44
  Description: Homo sapiens myosin heavy chain 4 (MYH4), mRNA. (from RefSeq NM_017533)
Gencode Transcript: ENST00000255381.2
Gencode Gene: ENSG00000264424.1
Transcript (Including UTRs)
   Position: hg38 chr17:10,443,290-10,469,559 Size: 26,270 Total Exon Count: 40 Strand: -
Coding Region
   Position: hg38 chr17:10,443,375-10,466,745 Size: 23,371 Coding Exon Count: 38 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:10,443,290-10,469,559)mRNA (may differ from genome)Protein (1939 aa)
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HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MYH4_HUMAN
DESCRIPTION: RecName: Full=Myosin-4; AltName: Full=Myosin heavy chain 2b; Short=MyHC-2b; AltName: Full=Myosin heavy chain 4; AltName: Full=Myosin heavy chain IIb; Short=MyHC-IIb; AltName: Full=Myosin heavy chain, skeletal muscle, fetal;
FUNCTION: Muscle contraction.
SUBUNIT: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
SUBCELLULAR LOCATION: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils.
DOMAIN: The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
DOMAIN: Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
SIMILARITY: Contains 1 IQ domain.
SIMILARITY: Contains 1 myosin head-like domain.
CAUTION: Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.48 RPKM in Muscle - Skeletal
Total median expression: 0.70 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.50111-0.149 Picture PostScript Text
3' UTR -20.9085-0.246 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000048 - IQ_motif_EF-hand-BS
IPR015650 - Myosin_1/23/4/7/8/13/15
IPR001609 - Myosin_head_motor_dom
IPR004009 - Myosin_N
IPR002928 - Myosin_tail

Pfam Domains:
PF00063 - Myosin head (motor domain)
PF02736 - Myosin N-terminal SH3-like domain
PF01576 - Myosin tail

ModBase Predicted Comparative 3D Structure on Q9Y623
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000146 microfilament motor activity
GO:0000166 nucleotide binding
GO:0003725 double-stranded RNA binding
GO:0003774 motor activity
GO:0003777 microtubule motor activity
GO:0003779 actin binding
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0008017 microtubule binding
GO:0016887 ATPase activity
GO:0051015 actin filament binding

Biological Process:
GO:0006936 muscle contraction
GO:0007018 microtubule-based movement
GO:0014823 response to activity
GO:0030048 actin filament-based movement
GO:0030049 muscle filament sliding
GO:0046034 ATP metabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005859 muscle myosin complex
GO:0016459 myosin complex
GO:0030016 myofibril
GO:0030017 sarcomere
GO:0032982 myosin filament


-  Descriptions from all associated GenBank mRNAs
  AF111783 - Homo sapiens myosin heavy chain IIb mRNA, complete cds.
BC157120 - Synthetic construct Homo sapiens clone IMAGE:100063492, MGC:190787 myosin, heavy chain 4, skeletal muscle (MYH4) mRNA, encodes complete protein.
BC156394 - Synthetic construct Homo sapiens clone IMAGE:100061945, MGC:190132 myosin, heavy chain 4, skeletal muscle (MYH4) mRNA, encodes complete protein.
JD517006 - Sequence 498030 from Patent EP1572962.
JD486679 - Sequence 467703 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04530 - Tight junction
hsa05416 - Viral myocarditis

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000255381.1, MYH4_HUMAN, NM_017533, Q9Y623, uc002gmn.1, uc002gmn.2, uc002gmn.3, uc002gmn.4
UCSC ID: ENST00000255381.2
RefSeq Accession: NM_017533
Protein: Q9Y623 (aka MYH4_HUMAN)
CCDS: CCDS11154.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.