Human Gene MIDN (ENST00000300952.7) from GENCODE V44
  Description: Homo sapiens midnolin (MIDN), mRNA. (from RefSeq NM_177401)
Gencode Transcript: ENST00000300952.7
Gencode Gene: ENSG00000167470.14
Transcript (Including UTRs)
   Position: hg38 chr19:1,248,553-1,259,143 Size: 10,591 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg38 chr19:1,250,297-1,257,272 Size: 6,976 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Methods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:1,248,553-1,259,143)mRNA (may differ from genome)Protein (468 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDMGIneXtProtOMIMPubMed
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MIDN_HUMAN
DESCRIPTION: RecName: Full=Midnolin; AltName: Full=Midbrain nucleolar protein;
FUNCTION: May be involved in regulation of genes related to neurogenesis in the nucleolus (By similarity).
SUBCELLULAR LOCATION: Nucleus, nucleolus (By similarity).
SIMILARITY: Contains 1 ubiquitin-like domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 51.87 RPKM in Fallopian Tube
Total median expression: 1302.13 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -261.50515-0.508 Picture PostScript Text
3' UTR -685.801871-0.367 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000626 - Ubiquitin
IPR019955 - Ubiquitin_supergroup

Pfam Domains:
PF00240 - Ubiquitin family

ModBase Predicted Comparative 3D Structure on Q504T8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0019900 kinase binding

Biological Process:
GO:0008150 biological_process
GO:0033132 negative regulation of glucokinase activity
GO:0046676 negative regulation of insulin secretion

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC094778 - Homo sapiens midnolin, mRNA (cDNA clone MGC:104683 IMAGE:30342357), complete cds.
BC060848 - Homo sapiens midnolin, mRNA (cDNA clone IMAGE:30347756), partial cds.
DL492366 - Novel nucleic acids.
DL490854 - Novel nucleic acids.
BC015089 - Homo sapiens midnolin, mRNA (cDNA clone IMAGE:3958934), partial cds.
AK075506 - Homo sapiens cDNA PSEC0204 fis, clone HEMBA1002706.
JD440361 - Sequence 421385 from Patent EP1572962.
JD456967 - Sequence 437991 from Patent EP1572962.
JD528710 - Sequence 509734 from Patent EP1572962.
JD528711 - Sequence 509735 from Patent EP1572962.
JD240075 - Sequence 221099 from Patent EP1572962.
JD366965 - Sequence 347989 from Patent EP1572962.
JD163599 - Sequence 144623 from Patent EP1572962.
JD123739 - Sequence 104763 from Patent EP1572962.
JD542112 - Sequence 523136 from Patent EP1572962.
JD307466 - Sequence 288490 from Patent EP1572962.
JD385997 - Sequence 367021 from Patent EP1572962.
JD124603 - Sequence 105627 from Patent EP1572962.
JD326654 - Sequence 307678 from Patent EP1572962.
JD102652 - Sequence 83676 from Patent EP1572962.
JD307407 - Sequence 288431 from Patent EP1572962.
JD445252 - Sequence 426276 from Patent EP1572962.
JD190927 - Sequence 171951 from Patent EP1572962.
JD126252 - Sequence 107276 from Patent EP1572962.
JD440900 - Sequence 421924 from Patent EP1572962.
JD172615 - Sequence 153639 from Patent EP1572962.
JD539817 - Sequence 520841 from Patent EP1572962.
JD192238 - Sequence 173262 from Patent EP1572962.
JD121951 - Sequence 102975 from Patent EP1572962.
JD136565 - Sequence 117589 from Patent EP1572962.
JD255626 - Sequence 236650 from Patent EP1572962.
JD561628 - Sequence 542652 from Patent EP1572962.
JD223109 - Sequence 204133 from Patent EP1572962.
JD560309 - Sequence 541333 from Patent EP1572962.
JD060884 - Sequence 41908 from Patent EP1572962.
JD486076 - Sequence 467100 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000300952.1, ENST00000300952.2, ENST00000300952.3, ENST00000300952.4, ENST00000300952.5, ENST00000300952.6, MIDN_HUMAN, NM_177401, Q504T8, Q96BW8, uc002lrp.1, uc002lrp.2, uc002lrp.3, uc002lrp.4, uc002lrp.5
UCSC ID: ENST00000300952.7
RefSeq Accession: NM_177401
Protein: Q504T8 (aka MIDN_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.