Human Gene FUT3 (ENST00000589620.6) from GENCODE V44
Description: Homo sapiens fucosyltransferase 3 (Lewis blood group) (FUT3), transcript variant 10, mRNA. (from RefSeq NM_001382748) RefSeq Summary (NM_001097639): The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000589620.6 Gencode Gene: ENSG00000171124.14 Transcript (Including UTRs) Position: hg38 chr19:5,842,888-5,851,449 Size: 8,562 Total Exon Count: 3 Strand: - Coding Region Position: hg38 chr19:5,843,754-5,844,839 Size: 1,086 Coding Exon Count: 1
ID:FUT3_HUMAN DESCRIPTION: RecName: Full=Galactoside 3(4)-L-fucosyltransferase; EC=2.4.1.65; AltName: Full=Blood group Lewis alpha-4-fucosyltransferase; Short=Lewis FT; AltName: Full=Fucosyltransferase 3; AltName: Full=Fucosyltransferase III; Short=FucT-III; FUNCTION: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. CATALYTIC ACTIVITY: GDP-beta-L-fucose + beta-D-galactosyl-(1->3)- N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha- L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R. PATHWAY: Protein modification; protein glycosylation. SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. TISSUE SPECIFICITY: Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. SIMILARITY: Belongs to the glycosyltransferase 10 family. WEB RESOURCE: Name=dbRBC/BGMUT; Note=Blood group antigen gene mutation database; URL="http://www.ncbi.nlm.nih.gov/gv/mhc/xslcgi.cgi?cmd=bgmut/systems_info&system=lewis"; WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=FUT3"; WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/fut3/"; WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=Fucosyltransferase 3; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_600";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00852 - Glycosyltransferase family 10 (fucosyltransferase) C-term
ModBase Predicted Comparative 3D Structure on P21217
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Mouse
Rat
Zebrafish
D. melanogaster
C. elegans
S. cerevisiae
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
Gene Ontology (GO) Annotations with Structured Vocabulary
BC011638 - Homo sapiens mRNA similar to chloride channel 7 (cDNA clone IMAGE:4111587). AF131913 - Homo sapiens alpha-(1,3/1,4)-fucosyltransferase (FT3B) mRNA, complete cds. X53578 - Human Lewis blood group locus mRNA for alpha(1,3/1,4)fucosyltransferase (EC 2.4.1.65). U27326 - Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, major transcript I, complete cds. U27327 - Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, major transcript II complete cds. U27328 - Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, minor transcript I, complete cds. JD043173 - Sequence 24197 from Patent EP1572962. JD502616 - Sequence 483640 from Patent EP1572962. JD284124 - Sequence 265148 from Patent EP1572962. JD044013 - Sequence 25037 from Patent EP1572962. JD179285 - Sequence 160309 from Patent EP1572962. JD533177 - Sequence 514201 from Patent EP1572962. JD291564 - Sequence 272588 from Patent EP1572962. JD472591 - Sequence 453615 from Patent EP1572962. JD432592 - Sequence 413616 from Patent EP1572962. JD316018 - Sequence 297042 from Patent EP1572962. JD053146 - Sequence 34170 from Patent EP1572962. JD178007 - Sequence 159031 from Patent EP1572962. JD348044 - Sequence 329068 from Patent EP1572962. JD220845 - Sequence 201869 from Patent EP1572962. JD054514 - Sequence 35538 from Patent EP1572962. JD513708 - Sequence 494732 from Patent EP1572962. BC108675 - Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group), mRNA (cDNA clone MGC:131739 IMAGE:5187745), complete cds. AK291852 - Homo sapiens cDNA FLJ78078 complete cds, highly similar to Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, minor transcript I. JD131480 - Sequence 112504 from Patent EP1572962. JD423584 - Sequence 404608 from Patent EP1572962. JD423585 - Sequence 404609 from Patent EP1572962. JD285934 - Sequence 266958 from Patent EP1572962. JD285933 - Sequence 266957 from Patent EP1572962. JD532984 - Sequence 514008 from Patent EP1572962. JD452010 - Sequence 433034 from Patent EP1572962. JD203482 - Sequence 184506 from Patent EP1572962. JD278698 - Sequence 259722 from Patent EP1572962. JD390461 - Sequence 371485 from Patent EP1572962. JD455243 - Sequence 436267 from Patent EP1572962. JD162797 - Sequence 143821 from Patent EP1572962. JD544083 - Sequence 525107 from Patent EP1572962. JD132014 - Sequence 113038 from Patent EP1572962. JD135045 - Sequence 116069 from Patent EP1572962. BC074836 - Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group), mRNA (cDNA clone MGC:103935 IMAGE:30915328), complete cds. BC074837 - Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group), mRNA (cDNA clone MGC:104096 IMAGE:30915548), complete cds. JD075905 - Sequence 56929 from Patent EP1572962. AK313575 - Homo sapiens cDNA, FLJ94139, highly similar to Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included) (FUT3), mRNA. KJ891204 - Synthetic construct Homo sapiens clone ccsbBroadEn_00598 FUT3 gene, encodes complete protein. KR712112 - Synthetic construct Homo sapiens clone CCSBHm_00035819 FUT3 (FUT3) mRNA, encodes complete protein. KR712113 - Synthetic construct Homo sapiens clone CCSBHm_00035823 FUT3 (FUT3) mRNA, encodes complete protein. KR712114 - Synthetic construct Homo sapiens clone CCSBHm_00035827 FUT3 (FUT3) mRNA, encodes complete protein. KR712115 - Synthetic construct Homo sapiens clone CCSBHm_00035830 FUT3 (FUT3) mRNA, encodes complete protein. JD115649 - Sequence 96673 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P21217 (Reactome details) participates in the following event(s):
R-HSA-5693925 FUT3 transfers L-fucose to Gal1,3GlcNAc R-HSA-975578 Reactions specific to the complex N-glycan synthesis pathway R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-446203 Asparagine N-linked glycosylation R-HSA-597592 Post-translational protein modification R-HSA-392499 Metabolism of proteins