Human Gene FUT3 (ENST00000589620.6) from GENCODE V44
  Description: Homo sapiens fucosyltransferase 3 (Lewis blood group) (FUT3), transcript variant 10, mRNA. (from RefSeq NM_001382748)
RefSeq Summary (NM_001097639): The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000589620.6
Gencode Gene: ENSG00000171124.14
Transcript (Including UTRs)
   Position: hg38 chr19:5,842,888-5,851,449 Size: 8,562 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg38 chr19:5,843,754-5,844,839 Size: 1,086 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:5,842,888-5,851,449)mRNA (may differ from genome)Protein (361 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCMalacardsMGIneXtProtOMIMPubMed
ReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FUT3_HUMAN
DESCRIPTION: RecName: Full=Galactoside 3(4)-L-fucosyltransferase; EC=2.4.1.65; AltName: Full=Blood group Lewis alpha-4-fucosyltransferase; Short=Lewis FT; AltName: Full=Fucosyltransferase 3; AltName: Full=Fucosyltransferase III; Short=FucT-III;
FUNCTION: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
CATALYTIC ACTIVITY: GDP-beta-L-fucose + beta-D-galactosyl-(1->3)- N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-(alpha- L-fucosyl-(1->4))-N-acetyl-beta-D-glucosaminyl-R.
PATHWAY: Protein modification; protein glycosylation.
SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi.
TISSUE SPECIFICITY: Highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver.
SIMILARITY: Belongs to the glycosyltransferase 10 family.
WEB RESOURCE: Name=dbRBC/BGMUT; Note=Blood group antigen gene mutation database; URL="http://www.ncbi.nlm.nih.gov/gv/mhc/xslcgi.cgi?cmd=bgmut/systems_info&system=lewis";
WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=FUT3";
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/fut3/";
WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=Fucosyltransferase 3; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_600";

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: FUT3
Diseases sorted by gene-association score: hypertropia (9), inflammatory breast carcinoma (9), colorectal cancer (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 55.88 RPKM in Esophagus - Mucosa
Total median expression: 190.52 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -73.50265-0.277 Picture PostScript Text
3' UTR -343.20866-0.396 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001503 - Glyco_trans_10

Pfam Domains:
PF00852 - Glycosyltransferase family 10 (fucosyltransferase) C-term

ModBase Predicted Comparative 3D Structure on P21217
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008417 fucosyltransferase activity
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0017060 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity
GO:0046920 alpha-(1->3)-fucosyltransferase activity

Biological Process:
GO:0006486 protein glycosylation
GO:0006672 ceramide metabolic process
GO:0009312 oligosaccharide biosynthetic process
GO:0009988 cell-cell recognition
GO:0036065 fucosylation
GO:0043413 macromolecule glycosylation

Cellular Component:
GO:0000139 Golgi membrane
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0032580 Golgi cisterna membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC011638 - Homo sapiens mRNA similar to chloride channel 7 (cDNA clone IMAGE:4111587).
AF131913 - Homo sapiens alpha-(1,3/1,4)-fucosyltransferase (FT3B) mRNA, complete cds.
X53578 - Human Lewis blood group locus mRNA for alpha(1,3/1,4)fucosyltransferase (EC 2.4.1.65).
U27326 - Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, major transcript I, complete cds.
U27327 - Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, major transcript II complete cds.
U27328 - Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, minor transcript I, complete cds.
JD043173 - Sequence 24197 from Patent EP1572962.
JD502616 - Sequence 483640 from Patent EP1572962.
JD284124 - Sequence 265148 from Patent EP1572962.
JD044013 - Sequence 25037 from Patent EP1572962.
JD179285 - Sequence 160309 from Patent EP1572962.
JD533177 - Sequence 514201 from Patent EP1572962.
JD291564 - Sequence 272588 from Patent EP1572962.
JD472591 - Sequence 453615 from Patent EP1572962.
JD432592 - Sequence 413616 from Patent EP1572962.
JD316018 - Sequence 297042 from Patent EP1572962.
JD053146 - Sequence 34170 from Patent EP1572962.
JD178007 - Sequence 159031 from Patent EP1572962.
JD348044 - Sequence 329068 from Patent EP1572962.
JD220845 - Sequence 201869 from Patent EP1572962.
JD054514 - Sequence 35538 from Patent EP1572962.
JD513708 - Sequence 494732 from Patent EP1572962.
BC108675 - Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group), mRNA (cDNA clone MGC:131739 IMAGE:5187745), complete cds.
AK291852 - Homo sapiens cDNA FLJ78078 complete cds, highly similar to Human alpha (1,3/1,4) fucosyltransferase (FUT3) mRNA, minor transcript I.
JD131480 - Sequence 112504 from Patent EP1572962.
JD423584 - Sequence 404608 from Patent EP1572962.
JD423585 - Sequence 404609 from Patent EP1572962.
JD285934 - Sequence 266958 from Patent EP1572962.
JD285933 - Sequence 266957 from Patent EP1572962.
JD532984 - Sequence 514008 from Patent EP1572962.
JD452010 - Sequence 433034 from Patent EP1572962.
JD203482 - Sequence 184506 from Patent EP1572962.
JD278698 - Sequence 259722 from Patent EP1572962.
JD390461 - Sequence 371485 from Patent EP1572962.
JD455243 - Sequence 436267 from Patent EP1572962.
JD162797 - Sequence 143821 from Patent EP1572962.
JD544083 - Sequence 525107 from Patent EP1572962.
JD132014 - Sequence 113038 from Patent EP1572962.
JD135045 - Sequence 116069 from Patent EP1572962.
BC074836 - Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group), mRNA (cDNA clone MGC:103935 IMAGE:30915328), complete cds.
BC074837 - Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group), mRNA (cDNA clone MGC:104096 IMAGE:30915548), complete cds.
JD075905 - Sequence 56929 from Patent EP1572962.
AK313575 - Homo sapiens cDNA, FLJ94139, highly similar to Homo sapiens fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included) (FUT3), mRNA.
KJ891204 - Synthetic construct Homo sapiens clone ccsbBroadEn_00598 FUT3 gene, encodes complete protein.
KR712112 - Synthetic construct Homo sapiens clone CCSBHm_00035819 FUT3 (FUT3) mRNA, encodes complete protein.
KR712113 - Synthetic construct Homo sapiens clone CCSBHm_00035823 FUT3 (FUT3) mRNA, encodes complete protein.
KR712114 - Synthetic construct Homo sapiens clone CCSBHm_00035827 FUT3 (FUT3) mRNA, encodes complete protein.
KR712115 - Synthetic construct Homo sapiens clone CCSBHm_00035830 FUT3 (FUT3) mRNA, encodes complete protein.
JD115649 - Sequence 96673 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P21217 (Reactome details) participates in the following event(s):

R-HSA-5693925 FUT3 transfers L-fucose to Gal1,3GlcNAc
R-HSA-975578 Reactions specific to the complex N-glycan synthesis pathway
R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi
R-HSA-948021 Transport to the Golgi and subsequent modification
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: B5U7U9, B5U7V0, ENST00000589620.1, ENST00000589620.2, ENST00000589620.3, ENST00000589620.4, ENST00000589620.5, FT3B, FUT3_HUMAN, LE, NM_001382748, P21217, Q32NE7, Q99448, Q99449, uc002mdm.1, uc002mdm.2, uc002mdm.3, uc002mdm.4
UCSC ID: ENST00000589620.6
RefSeq Accession: NM_001097639
Protein: P21217 (aka FUT3_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.