Human Gene MEIS1 (ENST00000272369.14) from GENCODE V44
  Description: Homo sapiens Meis homeobox 1 (MEIS1), mRNA. (from RefSeq NM_002398)
RefSeq Summary (NM_002398): Homeobox genes, of which the most well-characterized category is represented by the HOX genes, play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000272369.14
Gencode Gene: ENSG00000143995.20
Transcript (Including UTRs)
   Position: hg38 chr2:66,435,125-66,573,869 Size: 138,745 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg38 chr2:66,435,857-66,569,108 Size: 133,252 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:66,435,125-66,573,869)mRNA (may differ from genome)Protein (390 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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HGNCHPRDLynxMalacardsMGImyGene2
neXtProtOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MEIS1_HUMAN
DESCRIPTION: RecName: Full=Homeobox protein Meis1;
FUNCTION: Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
SUBUNIT: Interacts with the N-terminal region of PBX1 to form a heterodimer which binds DNA including a cAMP-responsive sequence in CYP17. Also forms heterodimers with PBX2. Forms heterotrimers with PBX1 or PBX2 and a number of HOX proteins including HOXA9, HOXD4 and HOXD9 where it acts as a non-DNA-binding partner. Also forms heterotrimers with PBX1 and HOX proteins including HOXD9 and HOXD10 where PBX1 is the non-DNA-binding partner.
INTERACTION: P16220:CREB1; NbExp=9; IntAct=EBI-1210694, EBI-711855; P14921:ETS1; NbExp=2; IntAct=EBI-1210694, EBI-913209;
SUBCELLULAR LOCATION: Nucleus (By similarity).
TISSUE SPECIFICITY: Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
DISEASE: Defects in MEIS1 could be a cause of susceptibility to restless legs syndrome type 7 (RLS7) [MIM:612853]. Restless legs syndrome (RLS) is a neurologic sleep/wake disorder characterized by uncomfortable and unpleasant sensations in the legs that appear at rest, usually at night, inducing an irresistible desire to move the legs. The disorder results in nocturnal insomnia and chronic sleep deprivation.
SIMILARITY: Belongs to the TALE/MEIS homeobox family.
SIMILARITY: Contains 1 homeobox DNA-binding domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MEIS1
Diseases sorted by gene-association score: restless legs syndrome (27), myeloid leukemia (14), restless legs syndrome 7 (14), hypocalciuric hypercalcemia, type ii (6), leukemia, acute myeloid (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 37.24 RPKM in Cervix - Ectocervix
Total median expression: 418.59 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -230.40732-0.315 Picture PostScript Text
3' UTR -692.302661-0.260 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008422 - Homeobox_KN_domain
IPR001356 - Homeodomain
IPR009057 - Homeodomain-like

Pfam Domains:
PF05920 - Homeobox KN domain

ModBase Predicted Comparative 3D Structure on O00470
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein SequenceProtein Sequence   
AlignmentAlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003705 transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
GO:0005515 protein binding
GO:0043565 sequence-specific DNA binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0001525 angiogenesis
GO:0002089 lens morphogenesis in camera-type eye
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0007275 multicellular organism development
GO:0007626 locomotory behavior
GO:0030097 hemopoiesis
GO:0035855 megakaryocyte development
GO:0045638 negative regulation of myeloid cell differentiation
GO:0045665 negative regulation of neuron differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048514 blood vessel morphogenesis
GO:0060044 negative regulation of cardiac muscle cell proliferation
GO:0060216 definitive hemopoiesis

Cellular Component:
GO:0005634 nucleus
GO:0005667 transcription factor complex


-  Descriptions from all associated GenBank mRNAs
  AL832770 - Homo sapiens mRNA; cDNA DKFZp686A197 (from clone DKFZp686A197).
BC043503 - Homo sapiens Meis homeobox 1, mRNA (cDNA clone MGC:49924 IMAGE:5531644), complete cds.
BC036511 - Homo sapiens Meis homeobox 1, mRNA (cDNA clone IMAGE:5266491), with apparent retained intron.
AK314949 - Homo sapiens cDNA, FLJ95861, highly similar to Homo sapiens Meis1, myeloid ecotropic viral integration site 1homolog (mouse) (MEIS1), mRNA.
U85707 - Human leukemogenic homolog protein (MEIS1) mRNA, complete cds.
AB527915 - Synthetic construct DNA, clone: pF1KB0940, Homo sapiens MEIS1 gene for Meis homeobox protein 1, without stop codon, in Flexi system.
AK297817 - Homo sapiens cDNA FLJ52704 complete cds, highly similar to Homeobox protein Meis1.
AK297109 - Homo sapiens cDNA FLJ54698 complete cds, highly similar to Homeobox protein Meis1.
AK098174 - Homo sapiens cDNA FLJ40855 fis, clone TRACH2016317, highly similar to HOMEOBOX PROTEIN MEIS1.
JD557727 - Sequence 538751 from Patent EP1572962.
JD345763 - Sequence 326787 from Patent EP1572962.
LF209007 - JP 2014500723-A/16510: Polycomb-Associated Non-Coding RNAs.
MA444584 - JP 2018138019-A/16510: Polycomb-Associated Non-Coding RNAs.
JD113502 - Sequence 94526 from Patent EP1572962.
JD116026 - Sequence 97050 from Patent EP1572962.
JD284896 - Sequence 265920 from Patent EP1572962.
JD046604 - Sequence 27628 from Patent EP1572962.
JD316142 - Sequence 297166 from Patent EP1572962.
JD251366 - Sequence 232390 from Patent EP1572962.
JD372578 - Sequence 353602 from Patent EP1572962.
JD290329 - Sequence 271353 from Patent EP1572962.
JD044614 - Sequence 25638 from Patent EP1572962.
JD232787 - Sequence 213811 from Patent EP1572962.
JD295923 - Sequence 276947 from Patent EP1572962.
JD268780 - Sequence 249804 from Patent EP1572962.
JD044322 - Sequence 25346 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O00470 (Reactome details) participates in the following event(s):

R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-5619507 Activation of HOX genes during differentiation
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: A8MV50, ENST00000272369.1, ENST00000272369.10, ENST00000272369.11, ENST00000272369.12, ENST00000272369.13, ENST00000272369.2, ENST00000272369.3, ENST00000272369.4, ENST00000272369.5, ENST00000272369.6, ENST00000272369.7, ENST00000272369.8, ENST00000272369.9, MEIS1_HUMAN, NM_002398, O00470, uc002sdu.1, uc002sdu.2, uc002sdu.3, uc002sdu.4, uc002sdu.5
UCSC ID: ENST00000272369.14
RefSeq Accession: NM_002398
Protein: O00470 (aka MEIS1_HUMAN or MEI1_HUMAN)
CCDS: CCDS46309.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.