Human Gene GRK4 (ENST00000398052.9) from GENCODE V44
Description: Homo sapiens G protein-coupled receptor kinase 4 (GRK4), transcript variant 1, mRNA. (from RefSeq NM_182982) RefSeq Summary (NM_182982): This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]. Gencode Transcript: ENST00000398052.9 Gencode Gene: ENSG00000125388.20 Transcript (Including UTRs) Position: hg38 chr4:2,963,571-3,040,760 Size: 77,190 Total Exon Count: 16 Strand: + Coding Region Position: hg38 chr4:2,964,071-3,040,625 Size: 76,555 Coding Exon Count: 16
ID:GRK4_HUMAN DESCRIPTION: RecName: Full=G protein-coupled receptor kinase 4; EC=2.7.11.16; AltName: Full=G protein-coupled receptor kinase GRK4; AltName: Full=ITI1; FUNCTION: Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. CATALYTIC ACTIVITY: ATP + [G-protein-coupled receptor] = ADP + [G- protein-coupled receptor] phosphate. ENZYME REGULATION: Inhibited by heparine. SUBUNIT: Interacts with DRD3. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cell cortex. Note=Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where co-localization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells. TISSUE SPECIFICITY: Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium. PTM: Palmitoylated. SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. SIMILARITY: Contains 1 AGC-kinase C-terminal domain. SIMILARITY: Contains 1 protein kinase domain. SIMILARITY: Contains 1 RGS domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00069 - Protein kinase domain PF00615 - Regulator of G protein signaling domain
ModBase Predicted Comparative 3D Structure on P32298
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
