Human Gene NKAIN1 (ENST00000373736.7) from GENCODE V44
  Description: Homo sapiens sodium/potassium transporting ATPase interacting 1 (NKAIN1), mRNA. (from RefSeq NM_024522)
RefSeq Summary (NM_024522): NKAIN1 is a member of a family of mammalian proteins with similarity to Drosophila Nkain and interacts with the beta subunit of Na,K-ATPase (ATP1B1; MIM 182330) (Gorokhova et al., 2007 [PubMed 17606467]).[supplied by OMIM, Jun 2009].
Gencode Transcript: ENST00000373736.7
Gencode Gene: ENSG00000084628.10
Transcript (Including UTRs)
   Position: hg38 chr1:31,179,745-31,239,887 Size: 60,143 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr1:31,181,703-31,239,547 Size: 57,845 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:31,179,745-31,239,887)mRNA (may differ from genome)Protein (207 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMGIneXtProtOMIM
PubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NKAI1_HUMAN
DESCRIPTION: RecName: Full=Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 1; Short=Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein 1; AltName: Full=Protein FAM77C; Flags: Precursor;
SUBUNIT: Interacts with ATP1B1 C-terminus (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the NKAIN family.
SEQUENCE CAUTION: Sequence=AAH96707.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH98173.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH98249.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAI31519.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAB14196.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=EAX07629.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.02 RPKM in Brain - Cerebellum
Total median expression: 102.21 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -207.60340-0.611 Picture PostScript Text
3' UTR -704.501958-0.360 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008516 - Na/K-Atpase_Interacting

Pfam Domains:
PF05640 - Na,K-Atpase Interacting protein

ModBase Predicted Comparative 3D Structure on Q4KMZ8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsembl  
Protein SequenceProtein SequenceProtein SequenceProtein Sequence  
AlignmentAlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0051117 ATPase binding

Biological Process:
GO:0002028 regulation of sodium ion transport

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK299507 - Homo sapiens cDNA FLJ54855 complete cds, moderately similar to Homo sapiens T-cell lymphoma breakpoint associated target 1 (TCBA1), mRNA.
AL832547 - Homo sapiens mRNA; cDNA DKFZp547I1617 (from clone DKFZp547I1617).
AK022712 - Homo sapiens cDNA FLJ12650 fis, clone NT2RM4002054.
JD231129 - Sequence 212153 from Patent EP1572962.
JD320031 - Sequence 301055 from Patent EP1572962.
JD082722 - Sequence 63746 from Patent EP1572962.
JD095496 - Sequence 76520 from Patent EP1572962.
JD412307 - Sequence 393331 from Patent EP1572962.
JD192685 - Sequence 173709 from Patent EP1572962.
JD104755 - Sequence 85779 from Patent EP1572962.
JD187911 - Sequence 168935 from Patent EP1572962.
JD388471 - Sequence 369495 from Patent EP1572962.
JD132154 - Sequence 113178 from Patent EP1572962.
JD534251 - Sequence 515275 from Patent EP1572962.
JD488039 - Sequence 469063 from Patent EP1572962.
JD374065 - Sequence 355089 from Patent EP1572962.
JD310349 - Sequence 291373 from Patent EP1572962.
JD478784 - Sequence 459808 from Patent EP1572962.
JD160587 - Sequence 141611 from Patent EP1572962.
JD324740 - Sequence 305764 from Patent EP1572962.
JD147799 - Sequence 128823 from Patent EP1572962.
AK298874 - Homo sapiens cDNA FLJ51944 complete cds, moderately similar to Homo sapiens T-cell lymphoma breakpoint associated target 1 (TCBA1), mRNA.
JD330625 - Sequence 311649 from Patent EP1572962.
JD120661 - Sequence 101685 from Patent EP1572962.
JD350369 - Sequence 331393 from Patent EP1572962.
JD090594 - Sequence 71618 from Patent EP1572962.
JD131177 - Sequence 112201 from Patent EP1572962.
JD565868 - Sequence 546892 from Patent EP1572962.
JD263048 - Sequence 244072 from Patent EP1572962.
JD058201 - Sequence 39225 from Patent EP1572962.
JD319728 - Sequence 300752 from Patent EP1572962.
JD393621 - Sequence 374645 from Patent EP1572962.
JD520116 - Sequence 501140 from Patent EP1572962.
JD132912 - Sequence 113936 from Patent EP1572962.
JD254334 - Sequence 235358 from Patent EP1572962.
JD484834 - Sequence 465858 from Patent EP1572962.
JD141682 - Sequence 122706 from Patent EP1572962.
JD175095 - Sequence 156119 from Patent EP1572962.
BC096707 - Homo sapiens Na+/K+ transporting ATPase interacting 1, mRNA (cDNA clone MGC:120143 IMAGE:40022279), complete cds.
BC098173 - Homo sapiens Na+/K+ transporting ATPase interacting 1, mRNA (cDNA clone MGC:120144 IMAGE:40022282), complete cds.
BC098249 - Homo sapiens Na+/K+ transporting ATPase interacting 1, mRNA (cDNA clone MGC:120142 IMAGE:40022276), complete cds.
BC131518 - Homo sapiens Na+/K+ transporting ATPase interacting 1, mRNA (cDNA clone MGC:141712 IMAGE:40022275), complete cds.
JD406893 - Sequence 387917 from Patent EP1572962.
JD129536 - Sequence 110560 from Patent EP1572962.
JD395080 - Sequence 376104 from Patent EP1572962.
JD133049 - Sequence 114073 from Patent EP1572962.
KJ903174 - Synthetic construct Homo sapiens clone ccsbBroadEn_12568 NKAIN1 gene, encodes complete protein.
JD534341 - Sequence 515365 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A2VDJ3, B7Z5W9, ENST00000373736.1, ENST00000373736.2, ENST00000373736.3, ENST00000373736.4, ENST00000373736.5, ENST00000373736.6, FAM77C, NKAI1_HUMAN, NM_024522, Q4KMZ8, Q9H9M7, uc010ogd.1, uc010ogd.2, uc010ogd.3, uc010ogd.4
UCSC ID: ENST00000373736.7
RefSeq Accession: NM_024522
Protein: Q4KMZ8 (aka NKAI1_HUMAN)
CCDS: CCDS339.2

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.