Human Gene MOGAT2 (ENST00000198801.10) from GENCODE V44
  Description: Homo sapiens monoacylglycerol O-acyltransferase 2 (MOGAT2), mRNA. (from RefSeq NM_025098)
RefSeq Summary (NM_025098): The protein encoded by this gene is an enzyme that catalyzes the synthesis of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. The encoded protein is important in the uptake of dietary fat by the small intestine. This protein forms a complex with diacylglycerol O-acyltransferase 2 in the endoplasmic reticulum, and this complex catalyzes the synthesis of triacylglycerol. [provided by RefSeq, Dec 2015].
Gencode Transcript: ENST00000198801.10
Gencode Gene: ENSG00000166391.15
Transcript (Including UTRs)
   Position: hg38 chr11:75,717,838-75,732,953 Size: 15,116 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg38 chr11:75,717,889-75,731,286 Size: 13,398 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:75,717,838-75,732,953)mRNA (may differ from genome)Protein (334 aa)
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-  Comments and Description Text from UniProtKB
  ID: MOGT2_HUMAN
DESCRIPTION: RecName: Full=2-acylglycerol O-acyltransferase 2; EC=2.3.1.22; AltName: Full=Acyl-CoA:monoacylglycerol acyltransferase 2; Short=MGAT2; Short=hMGAT2; AltName: Full=Diacylglycerol O-acyltransferase candidate 5; Short=hDC5; AltName: Full=Diacylglycerol acyltransferase 2-like protein 5; AltName: Full=Monoacylglycerol O-acyltransferase 2;
FUNCTION: Catalyzes the formation of diacylglycerol from 2- monoacylglycerol and fatty acyl-CoA. Has a preference toward monoacylglycerols containing unsaturated fatty acids in an order of C18:3 > C18:2 > C18:1 > C18:0. Plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. May play a role in diet-induced obesity.
CATALYTIC ACTIVITY: Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.
ENZYME REGULATION: Inhibited by oleic acid and sphingosine, while it is stimulated by phosphatidylcholine, phosphatidylserine and phosphatidic acid (By similarity).
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=45 uM for sn-1-monooleoylglycerol;
PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in liver, small intestine, colon, stomach and kidney.
SIMILARITY: Belongs to the diacylglycerol acyltransferase family.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 35.57 RPKM in Small Intestine - Terminal Ileum
Total median expression: 92.07 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -11.9051-0.233 Picture PostScript Text
3' UTR -553.001667-0.332 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007130 - DAGAT

Pfam Domains:
PF03982 - Diacylglycerol acyltransferase

ModBase Predicted Comparative 3D Structure on Q3SYC2
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
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Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence
AlignmentAlignmentAlignmentAlignmentAlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003846 2-acylglycerol O-acyltransferase activity
GO:0016407 acetyltransferase activity
GO:0016740 transferase activity
GO:0016746 transferase activity, transferring acyl groups
GO:0016747 transferase activity, transferring acyl groups other than amino-acyl groups

Biological Process:
GO:0006071 glycerol metabolic process
GO:0006629 lipid metabolic process
GO:0006651 diacylglycerol biosynthetic process
GO:0019432 triglyceride biosynthetic process
GO:0050892 intestinal absorption

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK026297 - Homo sapiens cDNA: FLJ22644 fis, clone HSI07088.
JD536563 - Sequence 517587 from Patent EP1572962.
AK291998 - Homo sapiens cDNA FLJ77354 complete cds, highly similar to Homo sapiens monoacylglycerol O-acyltransferase 2 (MOGAT2), mRNA.
KJ903283 - Synthetic construct Homo sapiens clone ccsbBroadEn_12677 MOGAT2 gene, encodes complete protein.
KJ894786 - Synthetic construct Homo sapiens clone ccsbBroadEn_04180 MOGAT2 gene, encodes complete protein.
AY157608 - Homo sapiens acyl CoA:monoacylglycerol acyltransferase 2 mRNA, complete cds; alternatively spliced.
BC103876 - Homo sapiens monoacylglycerol O-acyltransferase 2, mRNA (cDNA clone MGC:119183 IMAGE:40004273), complete cds.
BC103877 - Homo sapiens monoacylglycerol O-acyltransferase 2, mRNA (cDNA clone MGC:119184 IMAGE:40004282), complete cds.
BC103878 - Homo sapiens monoacylglycerol O-acyltransferase 2, mRNA (cDNA clone MGC:119185 IMAGE:40004283), complete cds.
AK128620 - Homo sapiens cDNA FLJ46779 fis, clone TRACH3027229, weakly similar to Homo sapiens diacylglycerol O-acyltransferase homolog 2 (mouse) (DGAT2).
JD135469 - Sequence 116493 from Patent EP1572962.
JD319093 - Sequence 300117 from Patent EP1572962.
JD382114 - Sequence 363138 from Patent EP1572962.
JD171646 - Sequence 152670 from Patent EP1572962.
AK000245 - Homo sapiens cDNA FLJ20238 fis, clone COLF5890.
JD152063 - Sequence 133087 from Patent EP1572962.
JD350649 - Sequence 331673 from Patent EP1572962.
JD499608 - Sequence 480632 from Patent EP1572962.
JD103433 - Sequence 84457 from Patent EP1572962.
JD066154 - Sequence 47178 from Patent EP1572962.
JD445962 - Sequence 426986 from Patent EP1572962.
JD079826 - Sequence 60850 from Patent EP1572962.
JD355885 - Sequence 336909 from Patent EP1572962.
JD242474 - Sequence 223498 from Patent EP1572962.
JD559991 - Sequence 541015 from Patent EP1572962.
JD182780 - Sequence 163804 from Patent EP1572962.
JD484383 - Sequence 465407 from Patent EP1572962.
JD318539 - Sequence 299563 from Patent EP1572962.
JD055715 - Sequence 36739 from Patent EP1572962.
JD392595 - Sequence 373619 from Patent EP1572962.
JD113413 - Sequence 94437 from Patent EP1572962.
JD214637 - Sequence 195661 from Patent EP1572962.
JD468996 - Sequence 450020 from Patent EP1572962.
JD195108 - Sequence 176132 from Patent EP1572962.
JD076012 - Sequence 57036 from Patent EP1572962.
JD398956 - Sequence 379980 from Patent EP1572962.
JD368493 - Sequence 349517 from Patent EP1572962.
JD422495 - Sequence 403519 from Patent EP1572962.
JD422496 - Sequence 403520 from Patent EP1572962.
JD409986 - Sequence 391010 from Patent EP1572962.
JD353275 - Sequence 334299 from Patent EP1572962.
JD150050 - Sequence 131074 from Patent EP1572962.
JD041106 - Sequence 22130 from Patent EP1572962.
JD296081 - Sequence 277105 from Patent EP1572962.
JD183219 - Sequence 164243 from Patent EP1572962.
JD270034 - Sequence 251058 from Patent EP1572962.
JD086418 - Sequence 67442 from Patent EP1572962.
JD299635 - Sequence 280659 from Patent EP1572962.
JD067713 - Sequence 48737 from Patent EP1572962.
JD423107 - Sequence 404131 from Patent EP1572962.
JD536837 - Sequence 517861 from Patent EP1572962.
JD524024 - Sequence 505048 from Patent EP1572962.
JD185493 - Sequence 166517 from Patent EP1572962.
JD096728 - Sequence 77752 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q3SYC2 (Reactome details) participates in the following event(s):

R-HSA-6800334 MOGAT1,2,3 transfer acyl group from acyl-CoA to 2-acylglycerol to form DAG
R-HSA-75109 Triglyceride biosynthesis
R-HSA-8979227 Triglyceride metabolism
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8K7I3, DC5, DGAT2L5, ENST00000198801.1, ENST00000198801.2, ENST00000198801.3, ENST00000198801.4, ENST00000198801.5, ENST00000198801.6, ENST00000198801.7, ENST00000198801.8, ENST00000198801.9, MOGT2_HUMAN, NM_025098, Q3SYC1, Q3SYC2, Q6ZQZ2, Q86UH6, Q9H630, uc010rru.1, uc010rru.2, uc010rru.3, uc010rru.4
UCSC ID: ENST00000198801.10
RefSeq Accession: NM_025098
Protein: Q3SYC2 (aka MOGT2_HUMAN)
CCDS: CCDS8240.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.