Human Gene ZNF577 (ENST00000301399.12) from GENCODE V44
  Description: Homo sapiens zinc finger protein 577 (ZNF577), transcript variant 1, mRNA. (from RefSeq NM_032679)
Gencode Transcript: ENST00000301399.12
Gencode Gene: ENSG00000161551.15
Transcript (Including UTRs)
   Position: hg38 chr19:51,867,048-51,887,951 Size: 20,904 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr19:51,872,532-51,880,382 Size: 7,851 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:51,867,048-51,887,951)mRNA (may differ from genome)Protein (485 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDMGIneXtProtPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ZN577_HUMAN
DESCRIPTION: RecName: Full=Zinc finger protein 577;
FUNCTION: May be involved in transcriptional regulation.
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.
SIMILARITY: Contains 8 C2H2-type zinc fingers.
SIMILARITY: Contains 1 KRAB domain.
CAUTION: It is uncertain whether Met-1 or Met-8 is the initiator.
SEQUENCE CAUTION: Sequence=AAH04992.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAF82168.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=EAW72049.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.11 RPKM in Ovary
Total median expression: 227.46 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -108.10366-0.295 Picture PostScript Text
3' UTR -1777.705484-0.324 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001909 - Krueppel-associated_box
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like
IPR013087 - Znf_C2H2/integrase_DNA-bd

Pfam Domains:
PF01352 - KRAB box
PF00096 - Zinc finger, C2H2 type

ModBase Predicted Comparative 3D Structure on Q9BSK1
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  BC035367 - Homo sapiens zinc finger protein 577, mRNA (cDNA clone IMAGE:5170465), with apparent retained intron.
AK056853 - Homo sapiens cDNA FLJ32291 fis, clone PROST2000572.
AK093036 - Homo sapiens cDNA FLJ35717 fis, clone TESTI2000598.
AX747873 - Sequence 1398 from Patent EP1308459.
BC004992 - Homo sapiens zinc finger protein 577, mRNA (cDNA clone MGC:4400 IMAGE:2905976), complete cds.
JD116035 - Sequence 97059 from Patent EP1572962.
JD564288 - Sequence 545312 from Patent EP1572962.
JD564287 - Sequence 545311 from Patent EP1572962.
JD336002 - Sequence 317026 from Patent EP1572962.
JD443221 - Sequence 424245 from Patent EP1572962.
JD380912 - Sequence 361936 from Patent EP1572962.
JD305715 - Sequence 286739 from Patent EP1572962.
JD066871 - Sequence 47895 from Patent EP1572962.
JD434080 - Sequence 415104 from Patent EP1572962.
JD510661 - Sequence 491685 from Patent EP1572962.
JD151534 - Sequence 132558 from Patent EP1572962.
JD411342 - Sequence 392366 from Patent EP1572962.
JD339342 - Sequence 320366 from Patent EP1572962.
JD301564 - Sequence 282588 from Patent EP1572962.
AL832871 - Homo sapiens mRNA; cDNA DKFZp667F053 (from clone DKFZp667F053).
JD094478 - Sequence 75502 from Patent EP1572962.
AK289479 - Homo sapiens cDNA FLJ76325 complete cds, highly similar to Homo sapiens zinc finger protein 577 (ZNF577), mRNA.
JD379408 - Sequence 360432 from Patent EP1572962.
JD410405 - Sequence 391429 from Patent EP1572962.
JD368255 - Sequence 349279 from Patent EP1572962.
AK291812 - Homo sapiens cDNA FLJ78406 complete cds.
KJ903460 - Synthetic construct Homo sapiens clone ccsbBroadEn_12854 ZNF577 gene, encodes complete protein.
EU446543 - Synthetic construct Homo sapiens clone IMAGE:100069882; IMAGE:100011752; FLH258591.01L zinc finger protein 577 (ZNF577) gene, encodes complete protein.
BC030610 - Homo sapiens zinc finger protein 577, mRNA (cDNA clone IMAGE:4826323), with apparent retained intron.
CU678844 - Synthetic construct Homo sapiens gateway clone IMAGE:100020723 5' read ZNF577 mRNA.
JD314308 - Sequence 295332 from Patent EP1572962.
JD317810 - Sequence 298834 from Patent EP1572962.
JD134066 - Sequence 115090 from Patent EP1572962.
JD297474 - Sequence 278498 from Patent EP1572962.
JD044451 - Sequence 25475 from Patent EP1572962.
JD546818 - Sequence 527842 from Patent EP1572962.
JD232605 - Sequence 213629 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BSK1 (Reactome details) participates in the following event(s):

R-HSA-975040 KRAB-ZNF / KAP Interaction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: A8K0B4, A8K6Z7, C9JFB9, ENST00000301399.1, ENST00000301399.10, ENST00000301399.11, ENST00000301399.2, ENST00000301399.3, ENST00000301399.4, ENST00000301399.5, ENST00000301399.6, ENST00000301399.7, ENST00000301399.8, ENST00000301399.9, NM_032679, Q9BSK1, uc010yde.1, uc010yde.2, uc010yde.3, uc010yde.4, uc010yde.5, ZN577_HUMAN
UCSC ID: ENST00000301399.12
RefSeq Accession: NM_001370447
Protein: Q9BSK1 (aka ZN577_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.