Human Gene CYP4F8 (ENST00000612078.5) from GENCODE V44
Description: Homo sapiens cytochrome P450 family 4 subfamily F member 8 (CYP4F8), mRNA. (from RefSeq NM_007253) RefSeq Summary (NM_007253): This gene, CYP4F8, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and functions as a 19-hydroxylase of prostaglandins in seminal vesicles. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F3, is approximately 18 kb away. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000612078.5 Gencode Gene: ENSG00000186526.13 Transcript (Including UTRs) Position: hg38 chr19:15,615,218-15,630,639 Size: 15,422 Total Exon Count: 13 Strand: + Coding Region Position: hg38 chr19:15,615,617-15,629,358 Size: 13,742 Coding Exon Count: 12
ID:CP4F8_HUMAN DESCRIPTION: RecName: Full=Cytochrome P450 4F8; EC=1.14.14.1; AltName: Full=CYPIVF8; FUNCTION: Hydroxylates arachidonic acid (20:4n-6) to (18R)- hydroxyarachidonate. Shows little activity against prostaglandin (PG) D2, PGE1, PGE2, PGF2alpha, and leukotriene B4. Catalyzes omega-2 and omega-3-hydroxylation of PGH1 and PGH2. Catalyzes epoxidation of 4,7,10,13,16,19-(Z)-docosahexaenoic acid (22:6n-3) and 7,10,13,16,19-(Z)-docosapentaenoic acid (22:5n-3) and omega-3- hydroxylation of 4,7,10,13,16-(Z)-docosapentaenoic acid (22:5n-6). Catalyzes hydroxylation of PGI2 and carbaprostacyclin. CATALYTIC ACTIVITY: RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O. COFACTOR: Heme group (By similarity). BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=7 uM for 9-alpha,11-alpha-epoxymethano-PGH2 (U-44069); KM=40 uM for 6,9-alpha-methylene-PGI2; Note=The Vmax of the reaction with U-44069 as substrate is 260 pmol/min/pmol enzyme; SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein (By similarity). Microsome membrane (By similarity). TISSUE SPECIFICITY: Expressed in the epithelium of seminal vesicles, in renal cortex, in adult and fetal liver, in epidermis, in corneal epithelium, in sweat glands, hair follicles, epithelial linings of the ampulla of vas deferens and of the stomach and small intestine, as well as in the transitional epithelium of the bladder and ureter (at protein level). In the epidermis, expressed from the basal cell to the granular cell layers. In the corneal epithelium, expressed in all cell layers. Also detected in prostate. Up-regulated in the epidermis of psoriatic lesions. SIMILARITY: Belongs to the cytochrome P450 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P98187
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Mouse
Rat
Zebrafish
D. melanogaster
C. elegans
S. cerevisiae
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0004497 monooxygenase activity GO:0005506 iron ion binding GO:0016491 oxidoreductase activity GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen GO:0018685 alkane 1-monooxygenase activity GO:0020037 heme binding GO:0046872 metal ion binding GO:0070330 aromatase activity
Biological Process: GO:0006690 icosanoid metabolic process GO:0006693 prostaglandin metabolic process GO:0055114 oxidation-reduction process
US Server
