Human Gene MDFIC (ENST00000393486.6) from GENCODE V44
Description: Homo sapiens MyoD family inhibitor domain containing (MDFIC), transcript variant 1, mRNA. (from RefSeq NM_199072) RefSeq Summary (NM_001166345): This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]. Gencode Transcript: ENST00000393486.6 Gencode Gene: ENSG00000135272.13 Transcript (Including UTRs) Position: hg38 chr7:114,922,094-115,019,917 Size: 97,824 Total Exon Count: 5 Strand: + Coding Region Position: hg38 chr7:114,923,034-115,015,935 Size: 92,902 Coding Exon Count: 4
ID:MDFIC_HUMAN DESCRIPTION: RecName: Full=MyoD family inhibitor domain-containing protein; AltName: Full=I-mfa domain-containing protein; Short=hIC; FUNCTION: Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin- beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. SUBUNIT: Interacts with HAND1; leading to sequester HAND1 into the nucleolus and prevent its activity. Interacts with ZIC2 (By similarity). The C-terminus interacts with HIV-1 Tat and Rev, AXIN1, the histidine-rich region of CCNT1/cyclin-T and weakly with LEF1. SUBCELLULAR LOCATION: Isoform 1: Nucleus, nucleolus. Note=Also shows a granular distribution in the cytoplasm. SUBCELLULAR LOCATION: Isoform 2: Cytoplasm. Note=Weak expression in the nucleus. TISSUE SPECIFICITY: Expressed in lymphoid organs (spleen, thymus, peripheral blood leukocytes) as well as prostate, uterus and small intestine. DOMAIN: The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation (By similarity). The cysteine-rich C- terminus is involved in its granular distribution in the cytoplasm. SIMILARITY: Belongs to the MDFI family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9P1T7
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0007257 activation of JUN kinase activity GO:0016032 viral process GO:0030111 regulation of Wnt signaling pathway GO:0042308 negative regulation of protein import into nucleus GO:0045892 negative regulation of transcription, DNA-templated GO:0045893 positive regulation of transcription, DNA-templated GO:0050434 positive regulation of viral transcription
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