S. cerevisiae Gene RPA14 (YDR156W)
  Description: RNA polymerase I subunit A14
Transcript (Including UTRs)
   Position: sacCer3 chrIV:769,525-769,938 Size: 414 Total Exon Count: 1 Strand: +
Coding Region
   Position: sacCer3 chrIV:769,525-769,938 Size: 414 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2011-08-29

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrIV:769,525-769,938)mRNAProtein (137 aa)
Gene SorterGenome BrowserOther Species FASTAAlphaFoldSGDUniProtKB

-  Comments and Description Text from UniProtKB
  ID: RPA14_YEAST
DESCRIPTION: RecName: Full=DNA-directed RNA polymerase I subunit RPA14; Short=A14; AltName: Full=DNA-directed RNA polymerase I 14 kDa polypeptide;
FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. A14 seems to play a role in the stability of subunits ABC23 and A43. In vitro, the A14-A43 subcomplex binds single-stranded RNA.
SUBUNIT: Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits. Part of a Pol I subcomplex consisting of the subunits A14 and A43. Interacts with RPA43.
SUBCELLULAR LOCATION: Nucleus, nucleolus.
PTM: The N-terminus is blocked.
MISCELLANEOUS: Present with 3100 molecules/cell in log phase SD medium.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013239 - RNA_polI_RPA14

Pfam Domains:
PF08203 - Yeast RNA polymerase I subunit RPA14

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2RF4 - X-ray


ModBase Predicted Comparative 3D Structure on P50106
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanMouseRatZebrafishD. melanogasterC. elegans
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001054 RNA polymerase I activity
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0005515 protein binding

Biological Process:
GO:0006360 transcription from RNA polymerase I promoter
GO:0042254 ribosome biogenesis
GO:0042790 transcription of nuclear large rRNA transcript from RNA polymerase I promoter

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005736 DNA-directed RNA polymerase I complex


-  Descriptions from all associated GenBank mRNAs
 

-  Other Names for This Gene
  UCSC ID: YDR156W
Protein: P50106 (aka RPA14_YEAST)

-  SGD Genes Methods, Credits, and Data Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.