Human Gene MESP1 (ENST00000300057.5) from GENCODE V43
  Description: Homo sapiens mesoderm posterior bHLH transcription factor 1 (MESP1), mRNA. (from RefSeq NM_018670)
Gencode Transcript: ENST00000300057.5
Gencode Gene: ENSG00000166823.6
Transcript (Including UTRs)
   Position: hg38 chr15:89,749,875-89,751,249 Size: 1,375 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg38 chr15:89,750,144-89,751,231 Size: 1,088 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesMethods
Data last updated at UCSC: 2023-02-17 13:02:02

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr15:89,749,875-89,751,249)mRNA (may differ from genome)Protein (268 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Mesoderm posterior protein 1; AltName: Full=Class C basic helix-loop-helix protein 5; Short=bHLHc5;
FUNCTION: Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways (By similarity).
MISCELLANEOUS: The N- and C-terminal domains are separated by a 2- repeat G-Q region.
SIMILARITY: Contains 1 bHLH (basic helix-loop-helix) domain.

-  Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

-  MalaCards Disease Associations
  MalaCards Gene Search: MESP1
Diseases sorted by gene-association score: tricuspid atresia (6), heart septal defect (4), conotruncal heart malformations (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 5.58 RPKM in Prostate
Total median expression: 67.41 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -2.3018-0.128 Picture PostScript Text
3' UTR -98.60269-0.367 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011598 - HLH_dom

Pfam Domains:
PF00010 - Helix-loop-helix DNA-binding domain

ModBase Predicted Comparative 3D Structure on Q9BRJ9
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein SequenceProtein Sequence   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0035326 enhancer binding
GO:0044212 transcription regulatory region DNA binding
GO:0046983 protein dimerization activity

Biological Process:
GO:0001707 mesoderm formation
GO:0001756 somitogenesis
GO:0001947 heart looping
GO:0003007 heart morphogenesis
GO:0003139 secondary heart field specification
GO:0003143 embryonic heart tube morphogenesis
GO:0003210 cardiac atrium formation
GO:0003211 cardiac ventricle formation
GO:0003236 sinus venosus morphogenesis
GO:0003241 growth involved in heart morphogenesis
GO:0003259 cardioblast anterior-lateral migration
GO:0003260 cardioblast migration
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0007219 Notch signaling pathway
GO:0007275 multicellular organism development
GO:0007369 gastrulation
GO:0008078 mesodermal cell migration
GO:0009880 embryonic pattern specification
GO:0022008 neurogenesis
GO:0023019 signal transduction involved in regulation of gene expression
GO:0035481 positive regulation of Notch signaling pathway involved in heart induction
GO:0042662 negative regulation of mesodermal cell fate specification
GO:0042664 negative regulation of endodermal cell fate specification
GO:0045446 endothelial cell differentiation
GO:0045747 positive regulation of Notch signaling pathway
GO:0045892 negative regulation of transcription, DNA-templated
GO:0045893 positive regulation of transcription, DNA-templated
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048368 lateral mesoderm development
GO:0051155 positive regulation of striated muscle cell differentiation
GO:0055007 cardiac muscle cell differentiation
GO:0060913 cardiac cell fate determination
GO:0060921 sinoatrial node cell differentiation
GO:0060947 cardiac vascular smooth muscle cell differentiation
GO:0060975 cardioblast migration to the midline involved in heart field formation
GO:0070368 positive regulation of hepatocyte differentiation
GO:0090082 positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway

Cellular Component:
GO:0005634 nucleus

-  Descriptions from all associated GenBank mRNAs
  AL357535 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 25865.
LF212121 - JP 2014500723-A/19624: Polycomb-Associated Non-Coding RNAs.
MA447698 - JP 2018138019-A/19624: Polycomb-Associated Non-Coding RNAs.
LF213091 - JP 2014500723-A/20594: Polycomb-Associated Non-Coding RNAs.
MA448668 - JP 2018138019-A/20594: Polycomb-Associated Non-Coding RNAs.
JD171198 - Sequence 152222 from Patent EP1572962.
AL357533 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1899308.
LF207735 - JP 2014500723-A/15238: Polycomb-Associated Non-Coding RNAs.
MA443312 - JP 2018138019-A/15238: Polycomb-Associated Non-Coding RNAs.
JD245144 - Sequence 226168 from Patent EP1572962.
AL357570 - Homo sapiens EST from clone 2321731, full insert.
AL357534 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 2250774.
BC006219 - Homo sapiens mesoderm posterior 1 homolog (mouse), mRNA (cDNA clone MGC:10676 IMAGE:3944350), complete cds.
AL357571 - Homo sapiens EST from clone 2238942, 5' end.
JD500486 - Sequence 481510 from Patent EP1572962.
JD522138 - Sequence 503162 from Patent EP1572962.
LF368475 - JP 2014500723-A/175978: Polycomb-Associated Non-Coding RNAs.
MA604052 - JP 2018138019-A/175978: Polycomb-Associated Non-Coding RNAs.
KJ905504 - Synthetic construct Homo sapiens clone ccsbBroadEn_15111 MESP1 gene, encodes complete protein.
EU446875 - Synthetic construct Homo sapiens clone IMAGE:100069823; IMAGE:100012084; FLH258594.01L mesoderm posterior 1 homolog (mouse) (MESP1) gene, encodes complete protein.
JD270816 - Sequence 251840 from Patent EP1572962.
LF213093 - JP 2014500723-A/20596: Polycomb-Associated Non-Coding RNAs.
MA448670 - JP 2018138019-A/20596: Polycomb-Associated Non-Coding RNAs.
JD382309 - Sequence 363333 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: BHLHC5, ENST00000300057.1, ENST00000300057.2, ENST00000300057.3, ENST00000300057.4, MESP1_HUMAN, NM_018670, Q9BRJ9, Q9NSF1, Q9NSF2, uc002bol.1, uc002bol.2, uc002bol.3, uc002bol.4, uc002bol.5
UCSC ID: ENST00000300057.5
RefSeq Accession: NM_018670
Protein: Q9BRJ9 (aka MESP1_HUMAN)

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.