Human Gene GNA13 (ENST00000439174.7) from GENCODE V44
  Description: Homo sapiens G protein subunit alpha 13 (GNA13), transcript variant 1, mRNA. (from RefSeq NM_006572)
Gencode Transcript: ENST00000439174.7
Gencode Gene: ENSG00000120063.10
Transcript (Including UTRs)
   Position: hg38 chr17:65,009,289-65,056,740 Size: 47,452 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg38 chr17:65,014,257-65,056,593 Size: 42,337 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:65,009,289-65,056,740)mRNA (may differ from genome)Protein (377 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: GNA13_HUMAN
DESCRIPTION: RecName: Full=Guanine nucleotide-binding protein subunit alpha-13; Short=G alpha-13; Short=G-protein subunit alpha-13;
FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts with HAX1.
INTERACTION: Q92888:ARHGEF1; NbExp=2; IntAct=EBI-465387, EBI-465400;
SUBCELLULAR LOCATION: Membrane; Lipid-anchor. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
PTM: Palmitoylation is critical for proper membrane localization and signaling.
PTM: Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.
SIMILARITY: Belongs to the G-alpha family. G(12) subfamily.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D000082 Acetaminophen
  • D016572 Cyclosporine
  • D004997 Ethinyl Estradiol
  • D013749 Tetrachlorodibenzodioxin
  • C049584 2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine
  • C054649 3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)-4-(3-hydroxypropyl)cyclohexanol
  • C027576 4-hydroxy-2-nonenal
  • C012606 4-vinyl-1-cyclohexene dioxide
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • D001564 Benzo(a)pyrene
          more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.75 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 567.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -83.90147-0.571 Picture PostScript Text
3' UTR -1201.104968-0.242 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000469 - Gprotein_alpha12
IPR001019 - Gprotein_alpha_su
IPR011025 - GproteinA_insert

Pfam Domains:
PF00503 - G-protein alpha subunit

ModBase Predicted Comparative 3D Structure on Q14344
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsemblEnsembl  
Protein SequenceProtein SequenceProtein SequenceProtein Sequence  
AlignmentAlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0001664 G-protein coupled receptor binding
GO:0003924 GTPase activity
GO:0004871 signal transducer activity
GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0019001 guanyl nucleotide binding
GO:0031683 G-protein beta/gamma-subunit complex binding
GO:0031702 type 1 angiotensin receptor binding
GO:0031752 D5 dopamine receptor binding
GO:0046872 metal ion binding

Biological Process:
GO:0001525 angiogenesis
GO:0001569 branching involved in blood vessel morphogenesis
GO:0001701 in utero embryonic development
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007189 adenylate cyclase-activating G-protein coupled receptor signaling pathway
GO:0007204 positive regulation of cytosolic calcium ion concentration
GO:0007266 Rho protein signal transduction
GO:0008360 regulation of cell shape
GO:0030154 cell differentiation
GO:0030168 platelet activation
GO:0030334 regulation of cell migration
GO:0031584 activation of phospholipase D activity
GO:0035556 intracellular signal transduction
GO:0043065 positive regulation of apoptotic process
GO:0051056 regulation of small GTPase mediated signal transduction

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005834 heterotrimeric G-protein complex
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0016020 membrane
GO:0031526 brush border membrane
GO:0042470 melanosome
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AB075500 - Homo sapiens neuroblastoma cDNA, clone:Nbla00250, full insert sequence.
AK026902 - Homo sapiens cDNA: FLJ23249 fis, clone COL04196.
BC036756 - Homo sapiens guanine nucleotide binding protein (G protein), alpha 13, mRNA (cDNA clone MGC:46138 IMAGE:5548632), complete cds.
AK002117 - Homo sapiens cDNA FLJ11255 fis, clone PLACE1008902.
BX647930 - Homo sapiens mRNA; cDNA DKFZp686I20201 (from clone DKFZp686I20201).
JD047339 - Sequence 28363 from Patent EP1572962.
JD496304 - Sequence 477328 from Patent EP1572962.
JD183462 - Sequence 164486 from Patent EP1572962.
JD516065 - Sequence 497089 from Patent EP1572962.
JD254402 - Sequence 235426 from Patent EP1572962.
JD227134 - Sequence 208158 from Patent EP1572962.
JD284885 - Sequence 265909 from Patent EP1572962.
JD166318 - Sequence 147342 from Patent EP1572962.
JD285732 - Sequence 266756 from Patent EP1572962.
JD530952 - Sequence 511976 from Patent EP1572962.
JD551823 - Sequence 532847 from Patent EP1572962.
JD508901 - Sequence 489925 from Patent EP1572962.
JD079241 - Sequence 60265 from Patent EP1572962.
JD338517 - Sequence 319541 from Patent EP1572962.
JD349494 - Sequence 330518 from Patent EP1572962.
JD510730 - Sequence 491754 from Patent EP1572962.
JD168094 - Sequence 149118 from Patent EP1572962.
JD231678 - Sequence 212702 from Patent EP1572962.
JD559735 - Sequence 540759 from Patent EP1572962.
JD355503 - Sequence 336527 from Patent EP1572962.
JD348325 - Sequence 329349 from Patent EP1572962.
AK302400 - Homo sapiens cDNA FLJ50732 complete cds, highly similar to Guanine nucleotide-binding protein alpha-13 subunit.
JD303297 - Sequence 284321 from Patent EP1572962.
JD551492 - Sequence 532516 from Patent EP1572962.
JD385358 - Sequence 366382 from Patent EP1572962.
L22075 - Homo sapiens guanine nucleotide regulatory protein (G13) mRNA, complete cds.
JD265223 - Sequence 246247 from Patent EP1572962.
AK301699 - Homo sapiens cDNA FLJ53108 complete cds, highly similar to Guanine nucleotide-binding protein alpha-13 subunit.
JD411326 - Sequence 392350 from Patent EP1572962.
JD196953 - Sequence 177977 from Patent EP1572962.
KJ893108 - Synthetic construct Homo sapiens clone ccsbBroadEn_02502 GNA13 gene, encodes complete protein.
AF493902 - Homo sapiens guanine nucleotide binding protein alpha 13 (GNA13) mRNA, complete cds.
AK313672 - Homo sapiens cDNA, FLJ94257, Homo sapiens guanine nucleotide binding protein (G protein), alpha 13 (GNA13), mRNA.
DQ893572 - Synthetic construct clone IMAGE:100006202; FLH170527.01X; RZPDo839G0498D guanine nucleotide binding protein (G protein), alpha 13 (GNA13) gene, encodes complete protein.
DQ894448 - Synthetic construct Homo sapiens clone IMAGE:100008908; FLH170523.01L; RZPDo839G0497D guanine nucleotide binding protein (G protein), alpha 13 (GNA13) gene, encodes complete protein.
AB528470 - Synthetic construct DNA, clone: pF1KB6581, Homo sapiens GNA13 gene for guanine nucleotide binding protein (G protein), alpha 13, without stop codon, in Flexi system.
CU691408 - Synthetic construct Homo sapiens gateway clone IMAGE:100019142 5' read GNA13 mRNA.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04270 - Vascular smooth muscle contraction
hsa04730 - Long-term depression
hsa04810 - Regulation of actin cytoskeleton

BioCarta from NCI Cancer Genome Anatomy Project
h_myosinPathway - PKC-catalyzed phosphorylation of inhibitory phosphoprotein of myosin phosphatase
h_Par1Pathway - Thrombin signaling and protease-activated receptors

Reactome (by CSHL, EBI, and GO)

Protein Q14344 (Reactome details) participates in the following event(s):

R-HSA-114548 G alpha (13) activates Rho guanine nucleotide exchange factor 1 (p115-RhoGEF)
R-HSA-428918 Dissociation of the TP:G13 complex
R-HSA-398184 LARG activation by G alpha 12/13
R-HSA-418574 G alpha (12/13) auto-inactivates by hydrolysing GTP to GDP
R-HSA-397891 Dissociation of the PAR:G12/13 complex
R-HSA-751019 The Ligand:GPCR:G12/13 complex dissociates
R-HSA-428909 Activated TP receptor binds G-protein G13
R-HSA-751039 Inactive G alpha (12/13) reassociates with G beta:gamma
R-HSA-114544 p115-RhoGEF activation of Rac1
R-HSA-428917 G13 activation by TP receptor
R-HSA-398185 LARG binds plexin B1
R-HSA-396941 Thrombin-activated PAR binds G-protein G12/13
R-HSA-751027 Liganded G12/13-activating GPCRs bind inactive heterotrimeric G-protein G12/13
R-HSA-194913 GEFs activate Rho GTPase:GDP
R-HSA-419166 GEFs activate RhoA,B,C
R-HSA-205039 p75NTR indirectly activates RAC and Cdc42 via a guanyl-nucleotide exchange factor
R-HSA-114552 G12/13 activation by PAR
R-HSA-751029 Liganded G12/13-activating GPCR acts as a GEF for G12/13
R-HSA-416482 G alpha (12/13) signalling events
R-HSA-428930 Thromboxane signalling through TP receptor
R-HSA-456926 Thrombin signalling through proteinase activated receptors (PARs)
R-HSA-388396 GPCR downstream signalling
R-HSA-392518 Signal amplification
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-372790 Signaling by GPCR
R-HSA-194840 Rho GTPase cycle
R-HSA-193648 NRAGE signals death through JNK
R-HSA-109582 Hemostasis
R-HSA-162582 Signal Transduction
R-HSA-194315 Signaling by Rho GTPases
R-HSA-204998 Cell death signalling via NRAGE, NRIF and NADE
R-HSA-193704 p75 NTR receptor-mediated signalling
R-HSA-73887 Death Receptor Signalling

-  Other Names for This Gene
  Alternate Gene Symbols: B2R977, ENST00000439174.1, ENST00000439174.2, ENST00000439174.3, ENST00000439174.4, ENST00000439174.5, ENST00000439174.6, GNA13_HUMAN, NM_006572, Q14344, Q8TD70, uc002jfc.1, uc002jfc.2, uc002jfc.3, uc002jfc.4, uc002jfc.5
UCSC ID: ENST00000439174.7
RefSeq Accession: NM_006572
Protein: Q14344 (aka GNA13_HUMAN or GB13_HUMAN)
CCDS: CCDS11661.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.