Human Gene MAFG (ENST00000357736.9) from GENCODE V44
  Description: Homo sapiens MAF bZIP transcription factor G (MAFG), transcript variant 1, mRNA. (from RefSeq NM_002359)
RefSeq Summary (NM_002359): Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010].
Gencode Transcript: ENST00000357736.9
Gencode Gene: ENSG00000197063.11
Transcript (Including UTRs)
   Position: hg38 chr17:81,918,270-81,927,735 Size: 9,466 Total Exon Count: 3 Strand: -
Coding Region
   Position: hg38 chr17:81,922,605-81,923,185 Size: 581 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:81,918,270-81,927,735)mRNA (may differ from genome)Protein (162 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MAFG_HUMAN
DESCRIPTION: RecName: Full=Transcription factor MafG; AltName: Full=V-maf musculoaponeurotic fibrosarcoma oncogene homolog G; AltName: Full=hMAF;
FUNCTION: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF- E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+) (By similarity).
SUBUNIT: Homodimer or heterodimer. Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3. Interacts with NFE2; the interaction results in tranactivation activation. Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Highly expressed in skeletal muscle. Also expressed in heart and brain.
PTM: Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.
PTM: Sumoylation at Lys-14 is required for active transcriptional repression (By similarity).
SIMILARITY: Belongs to the bZIP family. Maf subfamily.
SIMILARITY: Contains 1 bZIP (basic-leucine zipper) domain.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

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To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MAFG
Diseases sorted by gene-association score: fibrosarcoma (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D001564 Benzo(a)pyrene
  • D019256 Cadmium Chloride
  • C017947 sodium arsenite
  • C517041 (4-amino-1,4-dihydro-3-(2-pyridyl)-5-thioxo-1,2,4-triazole)copper(II)
  • C111118 2',3,3',4',5-pentachloro-4-hydroxybiphenyl
  • C023514 2,6-dinitrotoluene
  • D019840 2-Propanol
  • C035207 4-amino-2,6-dinitrotoluene
  • C027576 4-hydroxy-2-nonenal
  • C553817 7-(benzylamino)-1,3,4,8-tetrahydropyrrolo(4,3,2-de)quinolin-8(1H)-one
          more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.26 RPKM in Testis
Total median expression: 68.45 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -127.40237-0.538 Picture PostScript Text
3' UTR -1731.304335-0.399 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004827 - bZIP
IPR004826 - bZIP_Maf
IPR008917 - Euk_TF_DNA-bd
IPR024874 - Transciption_factor_Maf

Pfam Domains:
PF03131 - bZIP Maf transcription factor

ModBase Predicted Comparative 3D Structure on O15525
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0005515 protein binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0001701 in utero embryonic development
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0007596 blood coagulation
GO:0010628 positive regulation of gene expression
GO:0030534 adult behavior
GO:0030641 regulation of cellular pH
GO:0042127 regulation of cell proliferation
GO:0045604 regulation of epidermal cell differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm


-  Descriptions from all associated GenBank mRNAs
  LF384828 - JP 2014500723-A/192331: Polycomb-Associated Non-Coding RNAs.
MA620405 - JP 2018138019-A/192331: Polycomb-Associated Non-Coding RNAs.
AK130699 - Homo sapiens cDNA FLJ27189 fis, clone SYN02525.
BC006143 - Homo sapiens v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian), mRNA (cDNA clone IMAGE:3938680).
BC103722 - Homo sapiens cDNA clone IMAGE:6161387.
JD314785 - Sequence 295809 from Patent EP1572962.
JD144091 - Sequence 125115 from Patent EP1572962.
JD449472 - Sequence 430496 from Patent EP1572962.
JD487975 - Sequence 468999 from Patent EP1572962.
JD515750 - Sequence 496774 from Patent EP1572962.
JD084793 - Sequence 65817 from Patent EP1572962.
JD019103 - Sequence 127 from Patent EP1572962.
JD030329 - Sequence 11353 from Patent EP1572962.
JD089164 - Sequence 70188 from Patent EP1572962.
JD055743 - Sequence 36767 from Patent EP1572962.
JD280320 - Sequence 261344 from Patent EP1572962.
JD227281 - Sequence 208305 from Patent EP1572962.
JD161417 - Sequence 142441 from Patent EP1572962.
JD136530 - Sequence 117554 from Patent EP1572962.
JD195003 - Sequence 176027 from Patent EP1572962.
JD537454 - Sequence 518478 from Patent EP1572962.
JD049019 - Sequence 30043 from Patent EP1572962.
JD453567 - Sequence 434591 from Patent EP1572962.
JD164223 - Sequence 145247 from Patent EP1572962.
JD091660 - Sequence 72684 from Patent EP1572962.
JD295954 - Sequence 276978 from Patent EP1572962.
JD122841 - Sequence 103865 from Patent EP1572962.
JD515518 - Sequence 496542 from Patent EP1572962.
JD545733 - Sequence 526757 from Patent EP1572962.
JD142316 - Sequence 123340 from Patent EP1572962.
JD152513 - Sequence 133537 from Patent EP1572962.
JD088577 - Sequence 69601 from Patent EP1572962.
JD047986 - Sequence 29010 from Patent EP1572962.
DQ591919 - Homo sapiens piRNA piR-59031, complete sequence.
DQ588020 - Homo sapiens piRNA piR-55132, complete sequence.
Y11514 - Homo sapiens mRNA for transcription factor, Maf.
U84249 - Homo sapiens basic-leucine zipper transcription factor MafG (MAFG) mRNA, complete cds.
LF213780 - JP 2014500723-A/21283: Polycomb-Associated Non-Coding RNAs.
MA449357 - JP 2018138019-A/21283: Polycomb-Associated Non-Coding RNAs.
AF059195 - Homo sapiens basic-leucine zipper transcription factor MafG (MAFG) mRNA, complete cds.
BC012327 - Homo sapiens v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian), mRNA (cDNA clone MGC:20149 IMAGE:4637130), complete cds.
JD509549 - Sequence 490573 from Patent EP1572962.
JD396915 - Sequence 377939 from Patent EP1572962.
JD426352 - Sequence 407376 from Patent EP1572962.
JD439004 - Sequence 420028 from Patent EP1572962.
JD539769 - Sequence 520793 from Patent EP1572962.
JD387277 - Sequence 368301 from Patent EP1572962.
JD219578 - Sequence 200602 from Patent EP1572962.
JD181473 - Sequence 162497 from Patent EP1572962.
JD224393 - Sequence 205417 from Patent EP1572962.
JD259828 - Sequence 240852 from Patent EP1572962.
JD297992 - Sequence 279016 from Patent EP1572962.
JD528234 - Sequence 509258 from Patent EP1572962.
JD226700 - Sequence 207724 from Patent EP1572962.
DQ890709 - Synthetic construct clone IMAGE:100003339; FLH165340.01X; RZPDo839B02160D v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian) (MAFG) gene, encodes complete protein.
KJ891572 - Synthetic construct Homo sapiens clone ccsbBroadEn_00966 MAFG gene, encodes complete protein.
AB464741 - Synthetic construct DNA, clone: pF1KB8873, Homo sapiens MAFG gene for v-maf musculoaponeurotic fibrosarcoma oncogene homolog G, without stop codon, in Flexi system.
JD210601 - Sequence 191625 from Patent EP1572962.
JD142284 - Sequence 123308 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_arenrf2Pathway - Oxidative Stress Induced Gene Expression Via Nrf2

Reactome (by CSHL, EBI, and GO)

Protein O15525 (Reactome details) participates in the following event(s):

R-HSA-1008240 NFE2 is a heterodimer
R-HSA-1008200 NFE2 binds the beta globin locus control region
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
R-HSA-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000357736.1, ENST00000357736.2, ENST00000357736.3, ENST00000357736.4, ENST00000357736.5, ENST00000357736.6, ENST00000357736.7, ENST00000357736.8, MAFG_HUMAN, NM_002359, O15525, uc002kcn.1, uc002kcn.2, uc002kcn.3, uc002kcn.4, uc002kcn.5
UCSC ID: ENST00000357736.9
RefSeq Accession: NM_002359
Protein: O15525 (aka MAFG_HUMAN)
CCDS: CCDS11793.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.