Human Gene TSC22D4 (ENST00000300181.7) from GENCODE V44
  Description: Homo sapiens TSC22 domain family member 4 (TSC22D4), transcript variant 1, mRNA. (from RefSeq NM_030935)
RefSeq Summary (NM_030935): TSC22D4 is a member of the TSC22 domain family of leucine zipper transcriptional regulators (see TSC22D3; MIM 300506) (Kester et al., 1999 [PubMed 10488076]; Fiorenza et al., 2001 [PubMed 11707329]).[supplied by OMIM, Mar 2008].
Gencode Transcript: ENST00000300181.7
Gencode Gene: ENSG00000166925.9
Transcript (Including UTRs)
   Position: hg38 chr7:100,466,519-100,479,214 Size: 12,696 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg38 chr7:100,466,959-100,478,038 Size: 11,080 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:100,466,519-100,479,214)mRNA (may differ from genome)Protein (395 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMGIneXtProtOMIM
PubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: T22D4_HUMAN
DESCRIPTION: RecName: Full=TSC22 domain family protein 4; AltName: Full=TSC22-related-inducible leucine zipper protein 2; AltName: Full=Tsc-22-like protein THG-1;
FUNCTION: Transcriptional repressor.
SUBUNIT: Forms homodimer or heterodimer. Can form a heterodimer with TSC22D1.
INTERACTION: O95831:AIFM1; NbExp=2; IntAct=EBI-739485, EBI-356440; Q14145:KEAP1; NbExp=3; IntAct=EBI-739485, EBI-751001; Q15365:PCBP1; NbExp=2; IntAct=EBI-739485, EBI-946095; P84022:SMAD3; NbExp=2; IntAct=EBI-739485, EBI-347161; O75800:ZMYND10; NbExp=2; IntAct=EBI-739485, EBI-747061;
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the TSC-22/Dip/Bun family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 141.40 RPKM in Brain - Substantia nigra
Total median expression: 2217.25 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -294.60690-0.427 Picture PostScript Text
3' UTR -176.00440-0.400 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000580 - TSC-22_Dip_Bun

Pfam Domains:
PF01166 - TSC-22/dip/bun family

ModBase Predicted Comparative 3D Structure on Q9Y3Q8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI     
Protein Sequence     
Alignment     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0005515 protein binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0045892 negative regulation of transcription, DNA-templated
GO:0070236 negative regulation of activation-induced cell death of T cells

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  BC011974 - Homo sapiens cDNA clone IMAGE:4154342, containing frame-shift errors.
AJ133115 - Homo sapiens mRNA for TSC-22-like protein.
BC001966 - Homo sapiens TSC22 domain family, member 4, mRNA (cDNA clone MGC:5035 IMAGE:3457297), complete cds.
BC001486 - Homo sapiens TSC22 domain family, member 4, mRNA (cDNA clone MGC:1424 IMAGE:2966929), complete cds.
BC010406 - Homo sapiens TSC22 domain family, member 4, mRNA (cDNA clone MGC:15250 IMAGE:3832780), complete cds.
BC002972 - Homo sapiens TSC22 domain family, member 4, mRNA (cDNA clone MGC:1941 IMAGE:3538175), complete cds.
JD353611 - Sequence 334635 from Patent EP1572962.
JD394190 - Sequence 375214 from Patent EP1572962.
JD520088 - Sequence 501112 from Patent EP1572962.
AK297702 - Homo sapiens cDNA FLJ57331 complete cds, highly similar to TSC22 domain family protein 4.
JD355220 - Sequence 336244 from Patent EP1572962.
JD403017 - Sequence 384041 from Patent EP1572962.
JD394940 - Sequence 375964 from Patent EP1572962.
JD191588 - Sequence 172612 from Patent EP1572962.
JD541692 - Sequence 522716 from Patent EP1572962.
JD191130 - Sequence 172154 from Patent EP1572962.
JD129307 - Sequence 110331 from Patent EP1572962.
JD476977 - Sequence 458001 from Patent EP1572962.
JD423117 - Sequence 404141 from Patent EP1572962.
AK314824 - Homo sapiens cDNA, FLJ95703.
KJ894857 - Synthetic construct Homo sapiens clone ccsbBroadEn_04251 TSC22D4 gene, encodes complete protein.
KJ906341 - Synthetic construct Homo sapiens clone ccsbBroadEn_16011 TSC22D4 gene, encodes complete protein.
KJ906342 - Synthetic construct Homo sapiens clone ccsbBroadEn_16012 TSC22D4 gene, encodes complete protein.
DQ891268 - Synthetic construct clone IMAGE:100003898; FLH170593.01X; RZPDo839H0298D TSC22 domain family, member 4 (TSC22D4) gene, encodes complete protein.
EU176567 - Synthetic construct Homo sapiens clone IMAGE:100011384; FLH170592.01L; RZPDo839H11253D TSC22 domain family, member 4 (TSC22D4) gene, encodes complete protein.
AB464322 - Synthetic construct DNA, clone: pF1KB7660, Homo sapiens TSC22D4 gene for TSC22 domain family, member 4, without stop codon, in Flexi system.
AK296580 - Homo sapiens cDNA FLJ52209 complete cds.
BC031622 - Homo sapiens TSC22 domain family, member 4, mRNA (cDNA clone MGC:35267 IMAGE:5174360), complete cds.
CU679701 - Synthetic construct Homo sapiens gateway clone IMAGE:100017173 5' read TSC22D4 mRNA.
CU674964 - Synthetic construct Homo sapiens gateway clone IMAGE:100017176 5' read TSC22D4 mRNA.
AK310800 - Homo sapiens cDNA, FLJ17842.
JD394350 - Sequence 375374 from Patent EP1572962.
JD210355 - Sequence 191379 from Patent EP1572962.
JD547194 - Sequence 528218 from Patent EP1572962.
JD455391 - Sequence 436415 from Patent EP1572962.
JD128594 - Sequence 109618 from Patent EP1572962.
JD124685 - Sequence 105709 from Patent EP1572962.
JD518906 - Sequence 499930 from Patent EP1572962.
JD414605 - Sequence 395629 from Patent EP1572962.
JD152585 - Sequence 133609 from Patent EP1572962.
JD373267 - Sequence 354291 from Patent EP1572962.
JD191198 - Sequence 172222 from Patent EP1572962.
JD399977 - Sequence 381001 from Patent EP1572962.
JD400870 - Sequence 381894 from Patent EP1572962.
JD248301 - Sequence 229325 from Patent EP1572962.
JD218100 - Sequence 199124 from Patent EP1572962.
JD214864 - Sequence 195888 from Patent EP1572962.
JD381947 - Sequence 362971 from Patent EP1572962.
JD317447 - Sequence 298471 from Patent EP1572962.
JD295646 - Sequence 276670 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A4D2C3, D6W5V9, ENST00000300181.1, ENST00000300181.2, ENST00000300181.3, ENST00000300181.4, ENST00000300181.5, ENST00000300181.6, NM_030935, Q9Y3Q8, T22D4_HUMAN, THG1, TILZ2, uc003uva.1, uc003uva.2, uc003uva.3, uc003uva.4
UCSC ID: ENST00000300181.7
RefSeq Accession: NM_030935
Protein: Q9Y3Q8 (aka T22D4_HUMAN)
CCDS: CCDS5695.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.