Human Gene WEE2 (ENST00000397541.6) from GENCODE V44
  Description: Homo sapiens WEE2 oocyte meiosis inhibiting kinase (WEE2), mRNA. (from RefSeq NM_001105558)
Gencode Transcript: ENST00000397541.6
Gencode Gene: ENSG00000214102.7
Transcript (Including UTRs)
   Position: hg38 chr7:141,708,353-141,731,271 Size: 22,919 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg38 chr7:141,708,759-141,730,320 Size: 21,562 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:141,708,353-141,731,271)mRNA (may differ from genome)Protein (567 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WEE2_HUMAN
DESCRIPTION: RecName: Full=Wee1-like protein kinase 2; EC=2.7.10.2; AltName: Full=Wee1-like protein kinase 1B; AltName: Full=Wee1B kinase;
FUNCTION: Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation (By similarity).
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in testis.
PTM: Phosphorylation leads to increase its activity (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.25 RPKM in Testis
Total median expression: 3.39 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -101.40406-0.250 Picture PostScript Text
3' UTR -234.50951-0.247 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR008271 - Ser/Thr_kinase_AS
IPR017164 - Wee1-like_protein_kinase

Pfam Domains:
PF00069 - Protein kinase domain

ModBase Predicted Comparative 3D Structure on P0C1S8
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004672 protein kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0000278 mitotic cell cycle
GO:0006468 protein phosphorylation
GO:0007143 female meiotic division
GO:0016310 phosphorylation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0035038 female pronucleus assembly
GO:0045736 negative regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0051321 meiotic cell cycle
GO:0060631 regulation of meiosis I
GO:1900194 negative regulation of oocyte maturation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  AK131218 - Homo sapiens cDNA FLJ16107 fis, clone THYMU2004139, moderately similar to WEE1-LIKE PROTEIN KINASE (EC 2.7.1.112).
BC172300 - Synthetic construct Homo sapiens clone IMAGE:100068994, MGC:199005 WEE1 homolog 2 (S. pombe) (WEE2) mRNA, encodes complete protein.
JD167745 - Sequence 148769 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04110 - Cell cycle

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000397541.1, ENST00000397541.2, ENST00000397541.3, ENST00000397541.4, ENST00000397541.5, NM_001105558, P0C1S8, uc003vwn.1, uc003vwn.2, WEE1B, WEE2_HUMAN
UCSC ID: ENST00000397541.6
RefSeq Accession: NM_001105558
Protein: P0C1S8 (aka WEE2_HUMAN)
CCDS: CCDS43660.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.