Human Gene MAGEB4 (ENST00000378982.4) from GENCODE V44
  Description: Homo sapiens MAGE family member B4 (MAGEB4), mRNA. (from RefSeq NM_002367)
RefSeq Summary (NM_002367): This gene is a member of the MAGEB gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 68% sequence identity to each other. The promoters and first exons of the MAGEB genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEB genes are clustered on chromosome Xp22-p21. This gene sequence ends in the first intron of MAGEB1, another family member. This gene is expressed in testis. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000378982.4
Gencode Gene: ENSG00000120289.12
Transcript (Including UTRs)
   Position: hg38 chrX:30,242,000-30,244,187 Size: 2,188 Total Exon Count: 1 Strand: +
Coding Region
   Position: hg38 chrX:30,242,136-30,243,176 Size: 1,041 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:30,242,000-30,244,187)mRNA (may differ from genome)Protein (346 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MAGB4_HUMAN
DESCRIPTION: RecName: Full=Melanoma-associated antigen B4; AltName: Full=MAGE-B4 antigen;
TISSUE SPECIFICITY: Expressed in testis.
SIMILARITY: Contains 1 MAGE domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MAGEB4
Diseases sorted by gene-association score: melanoma (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -35.60136-0.262 Picture PostScript Text
3' UTR -240.701011-0.238 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002190 - MAGE
IPR021072 - Melanoma_ass_antigen_N

Pfam Domains:
PF01454 - MAGE family
PF12440 - Melanoma associated antigen family N terminal

ModBase Predicted Comparative 3D Structure on O15481
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Cellular Component:
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  AK313769 - Homo sapiens cDNA, FLJ94371, Homo sapiens melanoma antigen, family B, 4 (MAGEB4), mRNA.
BC032852 - Homo sapiens melanoma antigen family B, 4, mRNA (cDNA clone MGC:33144 IMAGE:5267183), complete cds.
JD402769 - Sequence 383793 from Patent EP1572962.
DQ891236 - Synthetic construct clone IMAGE:100003866; FLH170415.01X; RZPDo839F0298D melanoma antigen family B, 4 (MAGEB4) gene, encodes complete protein.
DQ894421 - Synthetic construct Homo sapiens clone IMAGE:100008881; FLH170411.01L; RZPDo839F0297D melanoma antigen family B, 4 (MAGEB4) gene, encodes complete protein.
KJ897162 - Synthetic construct Homo sapiens clone ccsbBroadEn_06556 MAGEB4 gene, encodes complete protein.
CR541779 - Homo sapiens full open reading frame cDNA clone RZPDo834E0330D for gene MAGEB4, melanoma antigen, family B, 4; complete cds, without stopcodon.
JD090514 - Sequence 71538 from Patent EP1572962.
JD272702 - Sequence 253726 from Patent EP1572962.
JD273825 - Sequence 254849 from Patent EP1572962.
JD306046 - Sequence 287070 from Patent EP1572962.
JD315461 - Sequence 296485 from Patent EP1572962.
JD063226 - Sequence 44250 from Patent EP1572962.
JD048217 - Sequence 29241 from Patent EP1572962.
JD286555 - Sequence 267579 from Patent EP1572962.
JD149946 - Sequence 130970 from Patent EP1572962.
JD562850 - Sequence 543874 from Patent EP1572962.
JD236832 - Sequence 217856 from Patent EP1572962.
JD449175 - Sequence 430199 from Patent EP1572962.
AK314977 - Homo sapiens cDNA, FLJ95893, highly similar to Homo sapiens melanoma antigen, family B, 1 (MAGEB1), mRNA.
X82539 - H.sapiens mRNA for MAGE-Xp.
JD288216 - Sequence 269240 from Patent EP1572962.
JD547006 - Sequence 528030 from Patent EP1572962.
JD068949 - Sequence 49973 from Patent EP1572962.
JD296230 - Sequence 277254 from Patent EP1572962.
JD563334 - Sequence 544358 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B2R9G0, ENST00000378982.1, ENST00000378982.2, ENST00000378982.3, MAGB4_HUMAN, NM_002367, O15481, Q6FHH4, Q8IZ00, uc004dcb.1, uc004dcb.2, uc004dcb.3, uc004dcb.4, uc004dcb.5
UCSC ID: ENST00000378982.4
RefSeq Accession: NM_002367
Protein: O15481 (aka MAGB4_HUMAN or MGB4_HUMAN)
CCDS: CCDS14221.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.