Human Gene RXFP4 (ENST00000368318.5) from GENCODE V44
  Description: Homo sapiens relaxin family peptide/INSL5 receptor 4 (RXFP4), mRNA. (from RefSeq NM_181885)
RefSeq Summary (NM_181885): GPR100 is a member of the rhodopsin family of G protein-coupled receptors (GPRs) (Fredriksson et al., 2003 [PubMed 14623098]).[supplied by OMIM, Mar 2008].
Gencode Transcript: ENST00000368318.5
Gencode Gene: ENSG00000173080.6
Transcript (Including UTRs)
   Position: hg38 chr1:155,941,638-155,943,087 Size: 1,450 Total Exon Count: 1 Strand: +
Coding Region
   Position: hg38 chr1:155,941,710-155,942,834 Size: 1,125 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2023-08-18 00:09:47

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:155,941,638-155,943,087)mRNA (may differ from genome)Protein (374 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGencodeGeneCards
HGNCHPRDLynxMalacardsMGIneXtProt
OMIMPubMedReactomeUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RL3R2_HUMAN
DESCRIPTION: RecName: Full=Relaxin-3 receptor 2; Short=RLN3 receptor 2; AltName: Full=G-protein coupled receptor 100; AltName: Full=G-protein coupled receptor GPCR142; AltName: Full=Insulin-like peptide INSL5 receptor; AltName: Full=Relaxin family peptide receptor 4;
FUNCTION: High affinity receptor for INSL5. Also acts as receptor for RLN3/relaxin-3, as well as bradykinin and kallidin. Binding of the ligand inhibit cAMP accumulation.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a broader range of tissues including brain, kidney, testis, thymus, placenta, prostate, salivary gland, thyroid and colon.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
SEQUENCE CAUTION: Sequence=BAC05844.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.72 RPKM in Colon - Transverse
Total median expression: 3.88 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -16.1072-0.224 Picture PostScript Text
3' UTR -78.80253-0.311 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR000248 - ATII_rcpt
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)

ModBase Predicted Comparative 3D Structure on Q8TDU9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGI     
Protein Sequence     
Alignment     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004966 galanin receptor activity
GO:0005515 protein binding

Biological Process:
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007188 adenylate cyclase-modulating G-protein coupled receptor signaling pathway
GO:0007200 phospholipase C-activating G-protein coupled receptor signaling pathway
GO:0007218 neuropeptide signaling pathway
GO:2000253 positive regulation of feeding behavior

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  BC101507 - Homo sapiens relaxin/insulin-like family peptide receptor 4, mRNA (cDNA clone MGC:126556 IMAGE:8069013), complete cds.
BC101509 - Homo sapiens relaxin/insulin-like family peptide receptor 4, mRNA (cDNA clone MGC:126558 IMAGE:8069015), complete cds.
KJ900620 - Synthetic construct Homo sapiens clone ccsbBroadEn_10014 RXFP4 gene, encodes complete protein.
AY170824 - Homo sapiens G protein-coupled receptor (GPR100) mRNA, complete cds.
AY288415 - Homo sapiens G protein-coupled receptor 100 (GPR100) mRNA, complete cds.
AY394502 - Homo sapiens relaxin-3 receptor-2 mRNA, complete cds.
EU432129 - Homo sapiens relaxin/insulin-like family peptide receptor 4 (RXFP4) mRNA, complete cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8TDU9 (Reactome details) participates in the following event(s):

R-HSA-444859 Relaxin 3 receptor 2 binds relaxin 3 and INSL5
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-444821 Relaxin receptors
R-HSA-418594 G alpha (i) signalling events
R-HSA-375276 Peptide ligand-binding receptors
R-HSA-388396 GPCR downstream signalling
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-372790 Signaling by GPCR
R-HSA-500792 GPCR ligand binding
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B0M0L4, ENST00000368318.1, ENST00000368318.2, ENST00000368318.3, ENST00000368318.4, GPR100, NM_181885, Q3MJB1, Q8NGZ8, Q8TDU9, RL3R2_HUMAN, RLN3R2, uc010pgs.1, uc010pgs.2, uc010pgs.3, uc010pgs.4
UCSC ID: ENST00000368318.5
RefSeq Accession: NM_181885
Protein: Q8TDU9 (aka RL3R2_HUMAN)
CCDS: CCDS1124.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.