Gene interactions and pathways from curated databases and text-mining
Proc Natl Acad Sci U S A 2001, PMID: 11438723

Visualization of biochemical networks in living cells.

Remy, I; Michnick, S W

Functional annotation of novel genes can be achieved by detection of interactions of their encoded proteins with known proteins followed by assays to validate that the gene participates in a specific cellular function. We report an experimental strategy that allows for detection of protein interactions and functional assays with a single reporter system. Interactions among biochemical network component proteins are detected and probed with stimulators and inhibitors of the network. In addition, the cellular location of the interacting proteins is determined. We used this strategy to map a signal transduction network that controls initiation of translation in eukaryotes. We analyzed 35 different pairs of full-length proteins and identified 14 interactions, of which five have not been observed previously, suggesting that the organization of the pathway is more ramified and integrated than previously shown. Our results demonstrate the feasibility of using this strategy in efforts of genomewide functional annotation.

Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Hprd Interaction: RPS6 — RPS6KB1 (in vitro)
  • IRef Hprd Interaction: PDPK1 — MTOR (in vitro)
  • IRef Hprd Interaction: MTOR — MTOR (in vitro)
  • IRef Hprd Interaction: MTOR — MTOR (in vivo)
  • IRef Hprd Interaction: AKT1 — RPS6KB1 (in vitro)
  • IRef Hprd Interaction: RPS6KB1 — RAC1 (in vitro)
  • IRef Hprd Interaction: RPS6KB1 — PPP2CA (in vivo)
  • IRef Hprd Interaction: RPS6KB1 — PPP2CA (in vitro)
  • IRef Hprd Interaction: CDC42 — RPS6KB1 (in vitro)
  • IRef Hprd Interaction: EIF4EBP1 — RPS6KB1 (in vivo)
  • IRef Hprd Interaction: AKT1 — PPP2CA (in vitro)
  • IRef Hprd Interaction: AKT1 — PPP2CA (in vivo)
  • IRef Hprd Interaction: RPS6KB1 — PDPK1 (in vivo)
  • IRef Hprd Interaction: RPS6KB1 — PDPK1 (in vitro)
  • IRef Hprd Interaction: EIF4EBP1 — MTOR (in vitro)
  • IRef Hprd Interaction: EIF4EBP1 — MTOR (in vivo)
  • IRef Hprd Interaction: AKT1 — MTOR (in vitro)
  • IRef Hprd Interaction: FKBP1A — FKBP1A (in vitro)
  • IRef Hprd Interaction: AKT1 — PDPK1 (in vitro)
  • IRef Hprd Interaction: AKT1 — PDPK1 (in vivo)
  • IRef Intact Interaction: PDPK1 — MTOR (physical association, beta lactamase complementation)
  • IRef Intact Interaction: MTOR — MTOR (physical association, beta lactamase complementation)
  • IRef Intact Interaction: AKT1 — RPS6KB1 (physical association, beta lactamase complementation)
  • IRef Intact Interaction: CDC42 — RPS6KB1 (physical association, beta lactamase complementation)
  • IRef Intact Interaction: PDPK1 — RPS6KB1 (physical association, beta lactamase complementation)
  • IRef Intact Interaction: AKT1 — MTOR (physical association, beta lactamase complementation)
  • IRef Intact Interaction: PDPK1 — AKT1 (physical association, beta lactamase complementation)
  • IRef Ophid Interaction: PDPK1 — MTOR (aggregation, confirmational text mining)
  • IRef Ophid Interaction: CDC42 — RPS6KB1 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: PDPK1 — RPS6KB1 (aggregation, confirmational text mining)
  • IRef Ophid Interaction: AKT1 — MTOR (aggregation, confirmational text mining)
In total, 14 gene pairs are associated to this article in curated databases