Gene interactions and pathways from curated databases and text-mining
Biochim Biophys Acta 2002, PMID: 11853878

Characterization of ubiquilin 1, an mTOR-interacting protein.

Wu, Shilan; Mikhailov, Alexei; Kallo-Hosein, Heidi; Hara, Kenta; Yonezawa, Kazuyoshi; Avruch, Joseph

The mTOR protein kinase is known to control cell cycle progression and cell growth through regulation of translation, transcription, membrane traffic and protein degradation. Known interactions of mTOR do not account for the multiple functions of this protein. Using a non-catalytic segment of mTOR (1-670) as bait in a yeast two-hybrid screen for interacting proteins, ubiquilin 1 (NM013438) was identified. Ubiquilin 1 is a member of a phylogenetically conserved gene family of unknown function, characterized by an N-terminal ubiquitin-like (Ubq) domain, a C-terminal ubiquitin associated (Uba) domain and a central region containing numerous NPXvar phi motifs (X, any; phi, hydrophobic amino acid). GST-ubiquilin 1 binds specifically to FLAG-mTOR (residues 1-670) in mammalian cells; residues 570-670 of mTOR and 226-323 of ubiquilin 1 are required for this interaction. Both mTOR and ubiquilin immunoreactivity appear as fine speckles throughout the cytoplasm; significant colocalization with cytoskeletal elements, early endosomes or proteasomes is not observed. As assessed by cell fractionation, mTOR is predominantly associated with low density membranes, along with 10% of ubiquilin 1. Ubiquilin 1 is a rapamycin-insensitive phosphoprotein. Overexpression of ubiquilin 1 does not alter the kinase activity of cotransfected mTOR or the phosphorylation of the mTOR target, p70 S6 kinase, in the presence or absence of rapamycin. Our data suggest that we have identified a novel mTOR interactor, ubiquilin 1. The biological significance of this, presumably membrane based, interaction, requires further study.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: UBQLN1 — MTOR (direct interaction, two hybrid)
  • IRef Biogrid Interaction: UBQLN1 — MTOR (physical association, affinity chromatography technology)
  • IRef Hprd Interaction: UBQLN1 — MTOR (two hybrid)
  • IRef Hprd Interaction: UBQLN1 — MTOR (in vivo)
  • IRef Hprd Interaction: UBQLN1 — MTOR (in vitro)
In total, 1 gene pairs are associated to this article in curated databases