J Neurosci 2004,
Takei, Nobuyuki; Inamura, Naoko; Kawamura, Mihoko; Namba, Hisaaki; Hara, Kenta; Yonezawa, Kazuyoshi; Nawa, Hiroyuki
In neurons, perisynaptic or dendritic translation is implicated in synapse-wide alterations of function and morphology triggered by neural activity. The molecular mechanisms controlling local translation activation, however, have yet to be elucidated. Here, we show that local protein synthesis and translational activation in neuronal dendrites are upregulated by brain-derived neurotrophic factor (BDNF) in a rapamycin and small interfering RNA specific for mammalian target of rapamycin (mTOR)-sensitive manner. In parallel, BDNF induced the phosphorylation of tuberin and the activation of mTOR in dendrites and the synaptoneurosome fraction. mTOR activation stimulated translation initiation processes involving both eIF4E/4E-binding protein (4EBP) and p70S6 kinase/ribosomal S6 protein. BDNF induced phosphorylation of 4EBP in isolated dendrites. Moreover, local puff application of BDNF to dendrites triggered S6 phosphorylation in a restricted area. Taken together, these data indicate that mTOR-dependent translation activation is essential for the upregulation of local protein synthesis in neuronal dendrites.
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Text Mining Data
Brain derived neurotrophic factor → mammalian target of rapamycin: " Brain derived neurotrophic factor
induces mammalian target of rapamycin
dependent local activation of translation machinery and protein synthesis in neuronal dendrites "
BDNF → mTOR: " In parallel, BDNF induced the phosphorylation of tuberin and the activation of mTOR in dendrites and the synaptoneurosome fraction "
BDNF → 4EBP: " BDNF induced phosphorylation of 4EBP in isolated dendrites "
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