FEBS Lett 2005,
PMID: 15620724
Tang, Chih-Hang Anthony; Hu, Chih-Chi Andrew; Wei, Chyou-Wei; Wang, Jaang-Jiun
Rana catesbeiana ribonuclease (RC-RNase) possesses tumor-specific cytotoxicity, which can be synergized by IFN-gamma. However, it is unclear how RC-RNase and RC-RNase/IFN-gamma induce cell death. In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and RC-RNase/IFN-gamma-induced caspase activation in HL-60, MCF-7, and SK-Hep-1 cells. We find that RC-RNase and RC-RNase/IFN-gamma induce mitochondria-mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions. Our findings provide evidence that RC-RNase and RC-RNase/IFN-gamma can kill different cancer cells by distinct mechanisms. Compared with onconase, RC-RNase seems to harbor a more specific anti-cancer activity.
Diseases/Pathways annotated by Medline MESH: Neoplasms
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Text Mining Data
caspase → RC-RNase/IFN-gamma: "
In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and
RC-RNase/IFN-gamma induced
caspase activation in HL-60, MCF-7, and SK-Hep-1 cells
"
caspase → RC-RNase/IFN-gamma: "
In this study, we use substrate cleavage assays to systematically investigate RC-RNase- and RC-RNase/IFN-gamma induced caspase activation in HL-60, MCF-7, and SK-Hep-1 cells
"
RC-RNase/IFN-gamma → caspase: "
We find that RC-RNase and RC-RNase/IFN-gamma induce mitochondria mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions
"
caspase → RC-RNase/IFN-gamma: "
We find that RC-RNase and RC-RNase/IFN-gamma induce mitochondria mediated caspase activation in HL-60 and MCF-7 cells but not in SK-Hep-1 cells, although death of SK-Hep-1 cells is closely related to mitochondrial disruptions
"
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