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Gene interactions and pathways from curated databases and text-mining
Biochem J 2007, PMID: 17461779

Identification of Protor as a novel Rictor-binding component of mTOR complex-2.

Pearce, Laura R; Huang, Xu; Boudeau, Jérôme; Pawłowski, Rafał; Wullschleger, Stephan; Deak, Maria; Ibrahim, Adel F M; Gourlay, Robert; Magnuson, Mark A; Alessi, Dario R

The mTOR (mammalian target of rapamycin) protein kinase is an important regulator of cell growth. Two complexes of mTOR have been identified: complex 1, consisting of mTOR-Raptor (regulatory associated protein of mTOR)-mLST8 (termed mTORC1), and complex 2, comprising mTOR-Rictor (rapamycininsensitive companion of mTOR)-mLST8-Sin1 (termed mTORC2). mTORC1 phosphorylates the p70 ribosomal S6K (S6 kinase) at its hydrophobic motif (Thr389), whereas mTORC2 phosphorylates PKB (protein kinase B) at its hydrophobic motif (Ser473). In the present study, we report that widely expressed isoforms of unstudied proteins termed Protor-1 (protein observed with Rictor-1) and Protor-2 interact with Rictor and are components of mTORC2. We demonstrate that immunoprecipitation of Protor-1 or Protor-2 results in the co-immunoprecipitation of other mTORC2 subunits, but not Raptor, a specific component of mTORC1. We show that detergents such as Triton X-100 or n-octylglucoside dissociate mTOR and mLST8 from a complex of Protor-1, Sin1 and Rictor. We also provide evidence that Rictor regulates the expression of Protor-1, and that Protor-1 is not required for the assembly of other mTORC2 subunits into a complex. Protor-1 is a novel Rictor-binding subunit of mTORC2, but further work is required to establish its role.

Document information provided by NCBI PubMed

Text Mining Data

Rictor → Protor-1: " We also provide evidence that Rictor regulates the expression of Protor-1, and that Protor-1 is not required for the assembly of other mTORC2 subunits into a complex "

Manually curated Databases

  • IRef Biogrid Interaction: RICTOR — MLST8 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — DHX36 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1B — RICTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — DHRS4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RPL13 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1A — RICTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — ABHD16A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RICTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — TIA1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA4 — RICTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RPS3A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — CLTC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — CPS1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — EHMT2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRR5 — MLST8 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRR5 — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRR5 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — PRR5L (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MAPKAP1 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — PRR5 (physical association, affinity chromatography technology)
  • IRef Intact Interaction: Complex of 27 proteins (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 13 proteins (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of PRR5-MTOR-YWHAE-YWHAB-HSPA1A-MLST8-RICTOR (association, tandem affinity purification)
  • IRef Intact Interaction: Complex of RPTOR-MAPKAP1-MTOR-MLST8-PRR5-RICTOR (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of 41 proteins (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of RICTOR-PRR5-MTOR-MAPKAP1-MLST8 (association, anti tag coimmunoprecipitation)
  • IRef Intact Interaction: Complex of MTOR-MLST8-RICTOR-MAPKAP1-PRR5L (association, anti tag coimmunoprecipitation)
  • NCI Pathway Database mTOR signaling pathway: mTORC2 complex (MTOR-MLST8-RICTOR-MAPKAP1-PRR5) → AKT1 (AKT1) (modification, activates)
    Evidence: mutant phenotype, assay, physical interaction
In total, 406 gene pairs are associated to this article in curated databases