Gene interactions and pathways from curated databases and text-mining
Ann N Y Acad Sci 2009, PMID: 19758219

Role for CaMKII inhibition in rheumatoid arthritis: effects on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.

Westra, Johanna; Brouwer, Elisabeth; Bouwman, Erwin; Doornbos-van der Meer, Berber; Posthumus, Marcel D; van Leeuwen, Miek A; Limburg, Pieter C; Ueda, Yutaka; Kallenberg, Cees G M

OBJECTIVE

The present study was designed to investigate the effects of a novel CaMKII inhibitor SMP-114, which was effective in a collagen-induced arthritis model in rats, on rheumatoid synovial fibroblasts.

METHODS

Rheumatoid synovial fibroblasts (RSF) were cultured under pro-inflammatory (IL-1beta stimulation) and hypoxic conditions. Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed.

RESULTS

Stimulation under hypoxic conditions induced higher expression of HIF-1alpha and VEGF mRNA than treatment with either IL-1beta or hypoxia alone. However, incubation with a specific CaMKII inhibitor did not result in reduced VEGF production in RSF.

CONCLUSIONS

CaMKII activation has been reported to be involved in HIF-1alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1-induced VEGF production by rheumatoid synovial fibroblasts.

Diseases/Pathways annotated by Medline MESH: Arthritis, Rheumatoid
Document information provided by NCBI PubMed

Text Mining Data

VEGF → HIF-1: " Role for CaMKII inhibition in rheumatoid arthritis : effects on HIF-1 induced VEGF production by rheumatoid synovial fibroblasts "

VEGF — HIF-1alpha: " Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed "

VEGF — PI3K-: " Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K- , and ERK pathway on VEGF and IL-6 production were analyzed "

VEGF — PI3K-: " Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K- , and ERK pathway on VEGF and IL-6 production were analyzed "

IL-6 — HIF-1alpha: " Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K-, and ERK pathway on VEGF and IL-6 production were analyzed "

IL-6 — PI3K-: " Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K- , and ERK pathway on VEGF and IL-6 production were analyzed "

IL-6 — PI3K-: " Protein and mRNA expression of HIF-1alpha and the effects of inhibitors of the CaMKII-, PI3K- , and ERK pathway on VEGF and IL-6 production were analyzed "

VEGF → HIF-1: " CaMKII activation has been reported to be involved in HIF-1alpha stabilization, but incubation with SMP-114, a specific CaMKII inhibitor, had no effect on HIF-1 induced VEGF production by rheumatoid synovial fibroblasts "

Manually curated Databases

No curated data.