Gene interactions and pathways from curated databases and text-mining
Cell Signal 2010, PMID: 20067833

High glucose induces suppression of insulin signalling and apoptosis via upregulation of endogenous IL-1beta and suppressor of cytokine signalling-1 in mouse pancreatic beta cells.

Venieratos, Panagiotis D; Drossopoulou, Garyfalia I; Kapodistria, Katerina D; Tsilibary, Effie C; Kitsiou, Paraskevi V

Chronic hyperglycemia and inflammatory cytokines disrupt and/or attenuate signal transduction pathways that promote normal beta-cell survival, leading to the destruction of endocrine pancreas in type 2 diabetes. There is convincing evidence that autocrine insulin signalling exerts protective anti-apoptotic effects on beta cells. Suppressors of cytokine signalling (SOCS) were induced by several cytokines and inhibit insulin-initiated signal transduction. The aim of this study was to investigate whether high glucose can influence endogenous interleukin-1beta (IL-1beta) and SOCS expression thus affecting insulin signalling and survival in insulin-producing mouse pancreatic beta cells (betaTC-6). Results showed that prolonged exposure of betaTC-6 cells to increased glucose concentrations resulted in significant inhibition of insulin-induced tyrosine phosphorylation of the insulin receptor (IR), and insulin receptor substrate-2 (IRS-2) as well as PI3-kinase activation. These changes were accompanied by impaired activation of the anti-apoptotic signalling protein Akt and annulment of Akt-mediated suppression of the Forkhead family of transcription factors (FoxO) activation. Glucose-induced attenuation of IRS-2/Akt-mediated signalling was associated with increased IL-1beta expression. Enhanced endogenous IL-1beta specifically induced mRNA and protein expression of SOCS-1 in betaTC-6 cells. Inhibition of SOCS-1 expression by SOCS-1-specific small interfering RNA restored IRS-2/PI3K-mediated Akt phosphorylation suppressed by high glucose. The upregulation of endogenous cytokine signalling and FoxO activation were accompanied by enhanced caspase-3 activation and increased susceptibility of cells to apoptosis. These results indicated that glucose-induced endogenous IL-1beta expression increased betaTC-6 cells apoptosis by inhibiting, at least in part, IRS-2/Akt-mediated signalling through SOCS-1 upregulation.

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Text Mining Data

insulin receptor (IR) → insulin: " Results showed that prolonged exposure of betaTC-6 cells to increased glucose concentrations resulted in significant inhibition of insulin induced tyrosine phosphorylation of the insulin receptor (IR) , and insulin receptor substrate-2 (IRS-2) as well as PI3-kinase activation "

insulin receptor substrate-2 (IRS-2) → insulin: " Results showed that prolonged exposure of betaTC-6 cells to increased glucose concentrations resulted in significant inhibition of insulin induced tyrosine phosphorylation of the insulin receptor (IR), and insulin receptor substrate-2 (IRS-2) as well as PI3-kinase activation "

PI3-kinase → insulin: " Results showed that prolonged exposure of betaTC-6 cells to increased glucose concentrations resulted in significant inhibition of insulin induced tyrosine phosphorylation of the insulin receptor (IR), and insulin receptor substrate-2 (IRS-2) as well as PI3-kinase activation "

PI3-kinase → insulin receptor substrate-2 (IRS-2): " Results showed that prolonged exposure of betaTC-6 cells to increased glucose concentrations resulted in significant inhibition of insulin induced tyrosine phosphorylation of the insulin receptor (IR), and insulin receptor substrate-2 (IRS-2) as well as PI3-kinase activation "

Akt ⊣ SOCS-1: " Inhibition of SOCS-1 expression by SOCS-1-specific small interfering RNA restored IRS-2/PI3K mediated Akt phosphorylation suppressed by high glucose "

Akt → IRS-2/PI3K: " Inhibition of SOCS-1 expression by SOCS-1-specific small interfering RNA restored IRS-2/PI3K mediated Akt phosphorylation suppressed by high glucose "

Akt → IRS-2/PI3K: " Inhibition of SOCS-1 expression by SOCS-1-specific small interfering RNA restored IRS-2/PI3K mediated Akt phosphorylation suppressed by high glucose "

SOCS-1 → IL-1beta: " These results indicated that glucose induced endogenous IL-1beta expression increased betaTC-6 cells apoptosis by inhibiting, at least in part, IRS-2/Akt mediated signalling through SOCS-1 upregulation "

IRS-2/Akt → IL-1beta: " These results indicated that glucose induced endogenous IL-1beta expression increased betaTC-6 cells apoptosis by inhibiting, at least in part, IRS-2/Akt mediated signalling through SOCS-1 upregulation "

IRS-2/Akt → IL-1beta: " These results indicated that glucose induced endogenous IL-1beta expression increased betaTC-6 cells apoptosis by inhibiting, at least in part, IRS-2/Akt mediated signalling through SOCS-1 upregulation "

IRS-2/Akt → IL-1beta: " These results indicated that glucose induced endogenous IL-1beta expression increased betaTC-6 cells apoptosis by inhibiting, at least in part, IRS-2/Akt mediated signalling through SOCS-1 upregulation "

Manually curated Databases

No curated data.