Gene interactions and pathways from curated databases and text-mining
FEBS Lett 2010, PMID: 20621840

Intracellular Ca2+ can compensate for the lack of NADPH oxidase-derived ROS in endothelial cells.

Lee, Monica; Spokes, Katherine C; Aird, William C; Abid, Md Ruhul

The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery endothelial cells (HCAEC). We also show that the selective inhibitory effects of NADPH oxidase knockdown on VEGF-mediated activation of c-Src-PI3K-Akt signaling and cell proliferation in HCAEC can be reversed by increase in intracellular Ca(2+). These results suggest an essential role for Ca(2+) in redox-dependent selective activation of c-Src-PI3K-Akt and endothelial cell proliferation.

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Text Mining Data

c-Src-PI3K-Akt → VEGF: " We demonstrate that reduction in Ca ( 2+ ) by chelating compound BAPTA-AM or by IP ( 3 ) -endoplasmic reticulum blocker 2-APB selectively inhibited VEGF induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery endothelial cells ( HCAEC ) "

ERK1/2 → VEGF: " We demonstrate that reduction in Ca ( 2+ ) by chelating compound BAPTA-AM or by IP ( 3 ) -endoplasmic reticulum blocker 2-APB selectively inhibited VEGF induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery endothelial cells ( HCAEC ) "

c-Src-PI3K-Akt → VEGF: " We demonstrate that reduction in Ca ( 2+ ) by chelating compound BAPTA-AM or by IP ( 3 ) -endoplasmic reticulum blocker 2-APB selectively inhibited VEGF induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery endothelial cells ( HCAEC ) "

c-Src-PI3K-Akt → VEGF: " We demonstrate that reduction in Ca ( 2+ ) by chelating compound BAPTA-AM or by IP ( 3 ) -endoplasmic reticulum blocker 2-APB selectively inhibited VEGF induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery endothelial cells ( HCAEC ) "

c-Src-PI3K-Akt → VEGF: " We also show that the selective inhibitory effects of NADPH oxidase knockdown on VEGF mediated activation of c-Src-PI3K-Akt signaling and cell proliferation in HCAEC can be reversed by increase in intracellular Ca ( 2+ ) "

c-Src-PI3K-Akt → VEGF: " We also show that the selective inhibitory effects of NADPH oxidase knockdown on VEGF mediated activation of c-Src-PI3K-Akt signaling and cell proliferation in HCAEC can be reversed by increase in intracellular Ca ( 2+ ) "

c-Src-PI3K-Akt → VEGF: " We also show that the selective inhibitory effects of NADPH oxidase knockdown on VEGF mediated activation of c-Src-PI3K-Akt signaling and cell proliferation in HCAEC can be reversed by increase in intracellular Ca ( 2+ ) "

Manually curated Databases

No curated data.