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UCSC Genome Browser Gene Interaction Graph
Gene interactions and pathways from curated databases and text-mining
EMBO J 2010, PMID: 21045808

mTORC2 can associate with ribosomes to promote cotranslational phosphorylation and stability of nascent Akt polypeptide.

Oh, Won Jun; Wu, Chang-chih; Kim, Sung Jin; Facchinetti, Valeria; Julien, Louis-André; Finlan, Monica; Roux, Philippe P; Su, Bing; Jacinto, Estela

The mechanisms that couple translation and protein processing are poorly understood in higher eukaryotes. Although mammalian target of rapamycin (mTOR) complex 1 (mTORC1) controls translation initiation, the function of mTORC2 in protein synthesis remains to be defined. In this study, we find that mTORC2 can colocalize with actively translating ribosomes and can stably interact with rpL23a, a large ribosomal subunit protein present at the tunnel exit. Exclusively during translation of Akt, mTORC2 mediates phosphorylation of the nascent polypeptide at the turn motif (TM) site, Thr450, to avoid cotranslational Akt ubiquitination. Constitutive TM phosphorylation occurs because the TM site is accessible, whereas the hydrophobic motif (Ser473) site is concealed in the ribosomal tunnel. Thus, mTORC2 can function cotranslationally by phosphorylating residues in nascent chains that are critical to attain proper conformation. Our findings reveal that mTOR links protein production with quality control.

Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: RICTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AKT1 — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3B — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3B — RPTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RPL23 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS6 — RPTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL26 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RPL23A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RPS6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS6 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL5 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL23 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — RPL26 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL23 — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS6 — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL23A — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRKCA — MTOR (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: RICTOR — RPS5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRKCA — UBC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AKT1 — MTOR (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: MAPKAP1 — MTOR (physical association, affinity chromatography technology)
  • IRef Intact Interaction: Complex of RPL26-MAPKAP1-RICTOR-RPS6-MTOR-RPL23A (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of MAPKAP1-RPS6-MTOR-RICTOR-RPL23A (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of MAPKAP1-MTOR-RPTOR-RICTOR (colocalization, cosedimentation through density gradient)
  • IRef Intact Interaction: Complex of RPTOR-RPS6-MTOR (association, anti bait coimmunoprecipitation)
In total, 30 gene pairs are associated to this article in curated databases