Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2011, PMID: 21177249

A new cytosolic pathway from a Parkinson disease-associated kinase, BRPK/PINK1: activation of AKT via mTORC2.

Murata, Hitoshi; Sakaguchi, Masakiyo; Jin, Yu; Sakaguchi, Yoshihiko; Futami, Jun-ichiro; Yamada, Hidenori; Kataoka, Ken; Huh, Nam-ho

Accumulating evidence indicates that dysfunction of mitochondria is a common feature of Parkinson disease. Functional loss of a familial Parkinson disease-linked gene, BRPK/PINK1 (PINK1), results in deterioration of mitochondrial functions and eventual neuronal cell death. A mitochondrial chaperone protein has been shown to be a substrate of PINK1 kinase activity. In this study, we demonstrated that PINK1 has another action point in the cytoplasm. Phosphorylation of Akt at Ser-473 was enhanced by overexpression of PINK1, and the Akt activation was crucial for protection of SH-SY5Y cells from various cytotoxic agents, including oxidative stress. Enhanced Akt phosphorylation was not due to activation of phosphatidylinositol 3-kinase but due to activation of mammalian target of rapamycin complex 2 (mTORC2) by PINK1. Rictor, a specific component of mTORC2, was phosphorylated by overexpression of PINK1. Furthermore, overexpression of PINK1 enhanced cell motility. These results indicate that PINK1 exerts its cytoprotective function not only in mitochondria but also in the cytoplasm through activation of mTORC2.

Diseases/Pathways annotated by Medline MESH: Neuroblastoma, Parkinson Disease, Prostatic Neoplasms
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Text Mining Data

AKT → mTORC2: " A new cytosolic pathway from a Parkinson disease associated kinase, BRPK/PINK1 : activation of AKT via mTORC2 "

Akt ⊣ PINK1: " Phosphorylation of Akt at Ser-473 was enhanced by overexpression of PINK1 , and the Akt activation was crucial for protection of SH-SY5Y cells from various cytotoxic agents, including oxidative stress "

PINK1 → mammalian target of rapamycin: " Enhanced Akt phosphorylation was not due to activation of phosphatidylinositol 3-kinase but due to activation of mammalian target of rapamycin complex 2 ( mTORC2 ) by PINK1 "

Akt → PINK1: " Enhanced Akt phosphorylation was not due to activation of phosphatidylinositol 3-kinase but due to activation of mammalian target of rapamycin complex 2 ( mTORC2 ) by PINK1 "

Akt → mammalian target of rapamycin: " Enhanced Akt phosphorylation was not due to activation of phosphatidylinositol 3-kinase but due to activation of mammalian target of rapamycin complex 2 ( mTORC2 ) by PINK1 "

Manually curated Databases

  • IRef Biogrid Interaction: RICTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AKT1 — RICTOR (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: RICTOR — PINK1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PINK1 — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AKT1 — PINK1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RICTOR — MAPKAP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: AKT1 — MAPKAP1 (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: AKT1 — MTOR (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: MAPKAP1 — MTOR (physical association, affinity chromatography technology)
  • Gene Ontology Complexes TORC2 complex: TORC2 complex complex (PINK1-RICTOR-TTI1-RPL23A)
In total, 15 gene pairs are associated to this article in curated databases