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EGF — KRT16
Text-mined interactions from Literome
Wang et al., J Biol Chem 2003
:
Induction of disease associated
keratin 16 gene expression by
epidermal growth factor is regulated through cooperation of transcription factors Sp1 and c-Jun ... Reporter assays revealed that the EGF response region was in the range of -162 to -114 bp. Disruption of the Sp1 site ( -127 to -122 bp ) and the AP1 site ( -148 to -142 bp ) of the keratin 16 promoter by site directed mutagenesis significantly inhibited
keratin 16 promoter activity
induced by
EGF ... Taken together, these results suggest that Sp1 and AP1 sites in the essential promoter region are critical for EGF response, and Sp1 showed a functional cooperation with c-Jun and coactivators p300/CBP in driving the transcriptional regulation of
EGF induced
keratin 16 gene expression
Wang et al., Mol Pharmacol 2006
:
In studies of gene regulation of keratin 16, we reported previously that simian virus 40 promoter factor 1 shows a functional cooperation with c-Jun and coactivators p300/CBP in driving the transcriptional regulation of
epidermal growth factor (EGF) induced
keratin 16 gene expression ... Ser63 and Ser73 on the c-Jun NH ( 2 ) -terminal transactivation domain could be phosphorylated in cells treated with EGF ; nevertheless, we found that the c-Jun COOH terminus played a pivotal role in
EGF induced expression of
keratin 16 ... p300 mediated and regulated
EGF induced
keratin 16 gene expression, at least in part, through multiple mechanisms, including a selective acetylation of c-Jun and histone H3
Chen et al., J Biol Chem 2007
:
ERK2 mediated C-terminal serine phosphorylation of p300 is vital to the regulation of
epidermal growth factor induced
keratin 16 gene expression ... We previously reported that the
epidermal growth factor (EGF) regulates the gene expression of
keratin 16 by activating the extracellular signal regulated kinase 1 and 2 ( ERK1/2 ) signaling which in turn enhances the recruitment of p300 to the keratin 16 promoter ... When all three serine residues on p300 were replaced by alanine,
EGF could no longer
induce the gene expression of
keratin 16 ... Taken together, these results strongly suggested that the ERK2 mediated C-terminal serine phosphorylation of p300 was a key event in the regulation of
EGF induced
keratin 16 expression
Obinata et al., Dev Biol 1987
:
EGF stimulated degradation of glucocorticoid induced
alpha-keratin
Aoyagi et al., J Invest Dermatol 1985
:
The analysis of phosphorylated phosphoamino acids revealed that
EGF stimulated tyrosine phosphorylation of pig epidermal fibrous
keratin
Magnaldo et al., DNA Cell Biol 1993
(Skin Diseases) :
Our previous studies have shown that the transcription of
K#16 is strongly and specifically
induced in epidermal keratinocytes by
epidermal growth factor (EGF) , through the EGF-responsive element ( RE )
Jiang et al., Proc Natl Acad Sci U S A 1993
:
Thus,
EGF specifically
induces K6 and
K16 , markers of activated keratinocytes, via nuclear proteins that bind to EGF-responsive elements in the promoters of these keratin genes
Tomić-Canić et al., Exp Cell Res 1996
:
Specifically, while
EGF induces expression of K6 and
K16 keratin genes, retinoic acid suppresses their expression, and when both mediators are present simultaneously, the level of expression is intermediate, a product of both signals