◀ Back to RHOA

DAAM1 — RHOA

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: RHOA → DAAM1 (increases, DAAM1 Activity) Habas et al., Cell 2001
  Evidence: Rho, Rac, and Cdc42 elicit distinct morphological changes in cultured mammalian cells (Hall, 1998). Specifically, Rho activation induces stress fiber formation. We found that N-Daam1 disrupts, whereas C-Daam1 enhances, the formation of actin stress fibers (Figure 4A). Thus, the cell biological assay is consistent with the biochemical evidence that Daam1 regulates RhoA activity.
• OpenBEL Selventa BEL large corpus: RHOA → DAAM1 (increases, DAAM1 Activity) Habas et al., Cell 2001
  Evidence: We also employed double-strand (ds) RNA mediated interference (RNAi) (Elbashir et al., 2001) to deplete endogenous Daam1 protein. The ds RNAi oligo for Daam1 reduces the protein level of Daam1, but not of RhoA, ?-catenin (Figure 3C) or Dvl2 (not shown). Importantly, the RNAi oligo inhibits RhoA activation by Wnt-1, Fz, or Dvl, but not by Ephexin (Figure 3C). A control ds RNAi oligo has no effect on Daam1 or RhoA protein level or RhoA activation (Figure 3C). Thus, Daam1 is essential for Wnt/Fz/Dv...
• OpenBEL Selventa BEL large corpus: RHOA → DAAM1 (increases, DAAM1 Activity, RHOA Activity) Katoh et al., Oncol Rep 2005*
  Evidence: Dishevelled-dependent WNT/PCP signals are transduced to the RHOA signaling cascade through Formin homology proteins DAAM1 and DAAM2, and to the JNK signaling cascade through MAPKKKs and MAPKK4/7
• KEGG Wnt signaling pathway: RHOA → DAAM1/DAAM2 (protein-protein, dissociation)
• NCI Pathway Database Noncanonical Wnt signaling pathway: DVL/DAAM1 complex (DVL3_DVL1_DVL2-DAAM1) → RHOA/GDP complex (RHOA) (modification, activates) Habas et al., Cell 2001, Endo et al., J Biol Chem 2005, Liu et al., Proc Natl Acad Sci U S A 2008, Yamamoto et al., Dev Cell 2008
  Evidence: mutant phenotype
• Reactome Reaction: RHOA → DAAM1 (indirect_complex) Habas et al., Cell 2001, Sagot et al., Nat Cell Biol 2002*, Watanabe et al., Exp Cell Res 2004*, Liu et al., Proc Natl Acad Sci U S A 2008, Tanegashima et al., EMBO J 2008, Lai et al., Cell Res 2009*, Amano et al., Cytoskeleton (Hoboken, N.J.) 2010*
• Reactome Reaction: RHOA → DAAM1 (reaction) Habas et al., Cell 2001, Sagot et al., Nat Cell Biol 2002*, Watanabe et al., Exp Cell Res 2004*, Liu et al., Proc Natl Acad Sci U S A 2008, Tanegashima et al., EMBO J 2008, Lai et al., Cell Res 2009*, Amano et al., Cytoskeleton (Hoboken, N.J.) 2010*
• WikiPathways Association Between Physico-Chemical Features and Toxicity Associated Pathways: RHOA → DAAM1 (unknown)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Dip Interaction: Complex of RHOA-DAAM1 (pull down) Liu et al., Proc Natl Acad Sci U S A 2008
• IRef Hprd Interaction: RHOA — DAAM1 (in vitro) Habas et al., Cell 2001
• IRef Hprd Interaction: RHOA — DAAM1 (in vivo) Habas et al., Cell 2001
• IRef Intact Interaction: Complex of RHOA-DIAPH1-DAAM1 (association, pull down) Higashi et al., J Biol Chem 2008
• IRef Intact Interaction: RHOA — DAAM1 (direct interaction, surface plasmon resonance) Higashi et al., J Biol Chem 2008
• IRef Intact Interaction: RHOA — DAAM1 (direct interaction, pull down) Higashi et al., J Biol Chem 2008
• IRef Intact Interaction: RHOA — DAAM1 (physical association, anti bait coimmunoprecipitation) Higashi et al., J Biol Chem 2008
• IRef Ophid Interaction: RHOA — DAAM1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Matusek et al., Development 2006 : Our results indicate that although DAAM is regulated by RhoA , it functions upstream or parallel to the non-receptor tyrosine kinases Src42A and Tec29 to organize the actin cytoskeleton and to determine the cuticle pattern of the Drosophila respiratory system
Zhu et al., PloS one 2012 (Breast Neoplasms) : Ectopic expression of N-Daam1 , a dominant negative mutant, or Daam1 siRNA remarkably inhibited Wnt5a induced RhoA activation, stress fiber formation and cell migration