◀ Back to CA2

CA2 — PLA2G1B

Text-mined interactions from Literome

Domínguez et al., Mol Reprod Dev 1999 : Our results suggest that GTP binding proteins require Ca+2 and PLA2 to accomplish their stimulatory effect, and that Ca+2 is also required when the acrosome reaction -- bypassing the action of PLA2 -- is stimulated by AA
Zheng et al., Immunology 1999 : Studies with selective inhibitors revealed that the FcgammaR mediated activation of PLA2 is dependent on Ca2+ and tyrosine kinase activity
Grotendorst et al., Toxicon 2000 (Hemolysis) : In common with all other known venoms and most secretory PLA2s, the beta PLA2 requires mM Ca2+ for optimal activity and is surface activated by aggregated lipids such as mixed micelles of detergent and phospholipid
Iijima et al., Lipids 2000 : G-1, G-2, and G-3 PLA2 had a pH optimum in an alkaline region at around pH 9-10 and required Ca2+ essentially for enzyme activity, using a mixed-micellar phosphatidylcholine substrate with sodium cholate
Beghini et al., J Protein Chem 2000 : The C. d. cascavella PLA2 required Ca2+ for activity, but was inhibited by Cu2+ and Zn2+ and by Cu2+ and Mg2+ in the presence and absence of Ca2+, respectively
Beghini et al., J Protein Chem 2000 : The PLA2 showed allosteric behavior, with maximal activity at pH 8.3 and 35-40 degrees C. C. d. cascavella PLA2 required Ca2+ for activity but was inhibited by Cu2+ and Zn2+ and by Cu2+ and Mg2+ in the presence and absence of Ca2+, respectively
Mizuno-Kamiya et al., Arch Oral Biol 2001 : The PLA2 in A-PM did not require Ca2+ for its activity and was highly activated by Triton X-100 and ATP
Olivero et al., Biochem Pharmacol 2001 : The effects of Ca2+ and Ca2+/calmodulin on the polychlorinated biphenyl (PCB) induced activation of phospholipase A2 (PLA2) in rat neutrophils were examined
Sauer et al., FASEB J 2001 (MAP Kinase Signaling System...) : ROS generation by ATP was inhibited by the P2 receptor antagonist suramin, by the reduced nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase inhibitors diphenylene iodonium chloride ( DPI ) and 4- ( 2-aminoethyl ) benzenesulfonylfluoride ( AEBSF ), as well as by the Ca2+ dependent phospholipase A2 (PLA2) inhibitors indomethacin and methyl arachidonyl fluorophosphonate ( MAFP )
Shin et al., Toxicol Appl Pharmacol 2002 : In the case of Ca2+, although 2,2',4,6-TeCB increased [Ca2+ ] i in the presence of extracellular Ca2+, PLA2 activation was not inhibited by EGTA and 1,2-bis ( o-aminophenoxy ) ethane-N, N,N ', N'-tetraacetic acid tetra ( acetoxy-methyl ) ester, an extracellular and an intracellular Ca2+ chelator, respectively
Boccellino et al., Lipids 2002 : The role of Ca2+ in PLA2 activation was also investigated
Shibata et al., J Leukoc Biol 1992 : The data suggest that increases in cellular Ca2+ directly activate membrane associated PLA2 and consequently initiate AA metabolism ; PKC activation by PMA requires additional steps to activate PLA2, a mechanism that is apparently deficient in the IL-3 dependent M phi-like cells
Garcia et al., J Lab Clin Med 1992 : Both the increase in [Ca2+ ] i and PKC activation are required for thrombin stimulated PLA2 and PLD activity, PGI2 synthesis, and barrier dysfunction, the latter occurring as the result of Ca2+ and PKC effects on specific cytoskeletal protein elements and other contractile proteins ( Fig. 3 )
Lee et al., Mol Cells 2003 : These data suggest that the purified enzyme is a novel Ca2+ dependent cytosolic PLA2
Gutiérrez et al., Toxicon 2003 : Upon binding, myotoxic PLA2s disrupt the integrity of the plasma membrane by catalytically dependent or independent mechanisms, provoking a pronounced Ca2+ influx which, in turn, initiates a complex series of degenerative events associated with hypercontraction, activation of calpains and cytosolic Ca ( 2+ ) -dependent PLA2s , and mitochondrial Ca2+ overload
Weerasinghe et al., Brain Res Bull 2004 : The involvement of Ca2+ dependent secretory PLA2 ( sPLA2 ) in the mechanism of action of lithium has not been investigated
Gentili et al., J Cell Biochem 2004 : Collectively, these data suggest that PTH activates in duodenal cells, a Ca2+ dependent cytosolic PLA2 and attendant AA release and that this activation requires prior stimulation of intracellular ERK1/2, PKA, and PKC
Damico et al., Biochim Biophys Acta 2005 : LmTX-I and LmTX-II had PLA2 activity in the presence of a synthetic substrate and showed a minimum sigmoidal behaviour ; with maximal activity at pH 8.0 and 35-45 degrees C. Full PLA2 activity required Ca2+ and was respectively inhibited by Cu2+ and Zn2+ in the presence and absence of Ca2+
Mirabet et al., J Mol Cell Cardiol 2005 (Calcium Signaling...) : Thrombin exerts multiple actions on cardiomyocytes leading to increased intracellular Na+ and Ca2+ concentrations, and to activation of a Ca2+ independent PLA2 , and has been proposed to favor the genesis of arrhythmias and ischemic injury in acute coronary syndromes
Rönkkö et al., Int J Biochem 1992 : 3. The purified PLA2 from human seminal plasma showed high affinity to heparin, sensitivity toward p-bromophenacyl bromide, Pb2+, dithioerythritol and EDTA and it was activated by Ca2+ and Mn2+
Hecquet et al., Am J Physiol Heart Circ Physiol 2006 : Because either a nonselective inhibitor ( isotetrandrine ) of phospholipase A2 (PLA2) or a Ca2+ dependent PLA2 inhibitor abolished potentiation of AA release by thrombin, while a Ca2+ independent PLA2 inhibitor did not, we concluded that the mechanism involves Ca2+ dependent PLA2 activation
Fujikawa et al., Lipids 2005 : It was discovered that the purified Nt PLA2 essentially requires Ca2+ , for the enzyme activity when the activity was determined using mixed-micellar phospholipid substrates with sodium cholate
Piomelli et al., Proc Natl Acad Sci U S A 1991 : This effect was accompanied by a marked decrease in the Vmax of the enzyme and little or no change in the Km. Furthermore, Ca2+/CaM with ATP but without exogenous Ca2+/CaM dependent PK II partially inhibited PLA2 activity, and this effect was prevented by treating the lysates with a selective peptide inhibitor of Ca2+/CaM dependent PK II ... Moreover, mutually antagonistic effects of the Ca2+/CaM dependent PK II and PLA2 pathways provide a possible molecular mechanism for bidirectional modulation of neurotransmitter release
Seleznev et al., J Biol Chem 2006 : Finally, we found that STS down-regulated endogenous iPLA2 transcription in both INS-1 and iPLA2 expressing INS-1 cells without affecting the expression of group IV Ca2+ dependent PLA2
Qu et al., Biochem Biophys Res Commun 2008 (Parvoviridae Infections) : The purified VP1 unique protein ( VP1U ) showed a typical Ca2+ dependent secreted PLA2-like ( sPLA2 ) activity, which was inhibited by sPLA2-specific inhibitors in a time dependent manner
Nakamura et al., J Clin Invest 1991 (Ischemia) : Cytosolic PLA2 activity was enhanced when Ca2+ concentration [( Ca2+]) was increased over the range of 10 ( -7 ) to 10 ( -6 ) M. Mitochondrial PLA2 activity required higher [ Ca2+ ] for activation ( greater than 10 ( -6 ) M )
Vargas-Villarreal et al., Toxicon 1991 : Phospholipase A2 from Heloderma horridum horridum and Crotalus adamanteus bind to the immobilized ligand in the presence of Ca2+ and can be easily eluted under acidic conditions or in the presence of a chelating agent, respectively
Diez et al., J Biol Chem 1990 (Leukemia, Myeloid...) : This suggests that membrane translocation and activation of the soluble PLA2 may be regulated by physiological intracellular levels of Ca2+
Tajuddin et al., Alcohol 2013 (Alcoholic Intoxication) : Analysis of HC with an immunoblot technique showed that AQP4, Ca(+2) dependent PLA2 ( cPLA2 IVA ), phosphorylated ( activated ) p-cPLA2, cleaved ( 89 kD ) PARP ( c-PARP ), and caspase-3 levels were significantly elevated over controls, whereas Ca(+2) independent PLA2 ( iPLA2 VIA ) was reduced ~70 % ; however, cleaved caspase-3 was undetectable
Karli et al., Proc Natl Acad Sci U S A 1990 : The final steps of secretion can thus be subdivided into a Ca2(+) dependent and -independent process : first, a Ca2 ( + ) -dependent activation of PLA2 liberating fatty acids from phospholipids and second, a Ca2 ( + ) -independent processing to the terminal fusogen and subsequent Ca2 ( + ) -independent fusion of the CGs with the PM
Stevens et al., Inflammation 1990 (Disease Models, Animal...) : PLA2 activities from both the fluid and cells require Ca2+ and have pH optima of 7
Lister et al., J Biol Chem 1989 : In order to ascertain the role of phospholipase A2 (PLA2) in the release of arachidonic acid for eicosanoid biosynthesis, we have characterized a Ca2+ dependent PLA2 from P388D1 cells, evaluated inhibitors of its activity, and correlated the effects of these inhibitors on prostaglandin ( PG ) E2 production in the intact cell ... The Ca2+ dependent PLA2 has little preference for the polar head group or sn-2 fatty acid of phospholipids, and we have now found that it will hydrolyze 1-alkyl,2-acyl phospholipids, but it does not show a preference for this substrate over other phospholipids ... Inhibitor studies with the Ca2+ dependent PLA2 have shown that arachidonic acid is an effective inhibitor
Hückel et al., Allerg Immunol (Leipz) 1989 : The different systems of signal transmission, in which such enzymes like Ca2+ dependent neutral Protease, Phospholipase C, Adenylatcyclase, Phospholipase A2 and Proteinkinases are involved, can interact
Franson et al., Mol Cell Biochem 1989 : To probe the possible role of lipid peroxidation in the regulation of myocardial lipid catabolism, a neutral-active and Ca2+ dependent PLA2 was extracted from rat heart and was partially purified by sulfopropyl cation exchange chromatography
Bonventre et al., J Clin Invest 1988 : Phospholipase A2 , the enzyme believed to be rate limiting for prostaglandin synthesis, is stimulated by Ca2+ ; however, the levels of Ca2+ necessary to stimulate phospholipase A2 in cell-free systems are higher than levels achieved in intact cells in response to agonists that stimulate prostaglandin synthesis
Fawzy et al., Agents Actions 1988 (Arthritis) : Retinoids, such as retinal, retinol, retinoic acid and retinol acetate, and flavonoids, such as quercetin, rutin, morin and sciadopitysin, inhibit Ca2+ dependent PLA2 activity of human synovial fluid ( HSF ) in vitro in a dose dependent fashion ; ID20S ranged from 2-8 microM ... Retinal inhibited neutral active Ca2+ dependent PLA2S from human platelets, human plasma, human polymorphonuclear leukocytes and Naja mossambica mossambica venom in a dose dependent manner while quercetin inhibits extracellular PLA2 activities of human plasma, HSF and N. m. mossambica venom in a dose dependent manner but not PLA2 activity derived from human platelets and polymorphomonuclear leukocytes ... Inhibition of PLA2 activity by both flavonoid and retinoids were independent of Ca2+ or Na+
Vishwanath et al., Inflammation 1988 (Edema) : A neutral-active, Ca2+ dependent phospholipase A2 (PLA2) purified 11,000-fold from human synovial fluid ( HSF ) induced edema when injected into the mouse foot pad ... These results clearly demonstrate that the neutral-active, Ca2+ dependent PLA2 isolated from human synovial fluid is proinflammatory and that catalytic activity is positively correlated with in vivo proinflammatory effects
Horigome et al., J Biochem 1987 : Phospholipase A2 shows a broad pH optimum ( greater than pH 7.0 ) and absolutely requires Ca2+
Hinkovska et al., Int J Biochem 1987 : Phospholipase A2 was optimally active at pH 7.4 and was Ca2+ dependent
Fawzy et al., Inflammation 1987 (Arthritis) : Cell-free, Ca2+ dependent phospholipase A2 activity (PLA2) was measured in human synovial fluid of patients with various kinds of arthritis using [ 1-14C ] oleate labelled autoclaved Escherichia coli as substrate ... Neutral active, Ca2+ dependent PLA2 activity in acid extracts of human platelets, plasma, polymorphonuclear leukocytes and synovial fluid varied in response to added Na+ ... In presence of 150 mM added Na+ and 5 mM Ca2+ , PLA2 activity in human synovial fluid was inhibited by all multivalent cations tested
Gamache et al., Biochim Biophys Acta 1986 : Phospholipase A2 activity ( 64.3-545.6 nmol phospholipid hydrolyzed per min per mg protein ) was optimal in the neutral to alkaline pH range, was Ca2+ dependent , and was unaffected by the addition of xanthine
Moskowitz et al., Biochem Biophys Res Commun 1983 : Phospholipase A2 in the presence of Ca2+ was stimulated by calmodulin and by prostaglandin F2 alpha
Saxon et al., Basic Res Cardiol 1984 : The data obtained suggested that modulation of Ca2+ dependent PL A2 activity may contribute significantly to membrane destabilization due to Ca2+ overload of cardiac cells
Ballou et al., Proc Natl Acad Sci U S A 1983 : PLA2 activity was Ca2+ dependent
Hirasawa et al., J Immunol 1995 : This and previous findings suggested that release of arachidonic acid was attributable to the regulation of cytosolic PLA2 by MAP kinase ( for activation of PLA2 ) and Ca2+ ( for association of PLA2 with the membrane ), whereas release of hexosaminidase was regulated primarily by Ca2+ and protein kinase C
Shimizu et al., Pancreas 1994 : This promoting effect of pancreatic and venom PLA2 required Ca2+ in the incubation medium and was inhibited by mepacrine ( 10 ( -5 ) M )
Nishida et al., Res Commun Chem Pathol Pharmacol 1993 : The effect of gamma-GCE on cell injury, as estimated by release of transaminases from cells, and changes in phospholipase A2 (PLA2) activity and of GSH, Ca2+ , and lipid peroxide (LPO) contents were examined in isolated rat hepatocytes treated with anti-rat liver plasma membrane rabbit antiserum ( ALPMA ) and compared with that of GSH
Sharma et al., Oncogene 1993 (Cell Transformation, Viral) : Both melittin- and A23187 mediated PLA2 hyperactivation require Ca2+
Reynolds et al., Biochim Biophys Acta 1993 : The PLA2 activity of this enzyme is stimulated by microM levels of Ca2+ ... In the absence of Ca2+, high salt concentrations overcame the requirement for divalent metals, indicating that Ca2+ is not required for PLA2 catalytic activity
Choi et al., Am J Physiol 1995 (Anoxia) : All PLA2 activity detected during normoxia was Ca2+ dependent
Tsunoda et al., Am J Physiol 1996 (Second Messenger Systems) : In contrast, JMV-180- and OPE stimulated Ca2+ oscillations were inhibited by the PLA2 , but not by the PLC, inhibitor
Huang et al., Biochemistry 1996 : This is in contrast to the WT PLA2 for which Ca2+ binding enhances binding of the substrate to the active site
McHowat et al., Am J Physiol 1997 : In the presence of Ca2+ with phosphatidylcholine, IL-1beta had no effect on membrane associated PLA2 but decreased cytosolic PLA2 activity
Sonnino et al., Dig Dis Sci 1997 (Reperfusion Injury) : In controls, Ca2+ dependent , secretory PLA2 activity accumulated rapidly during the first 6 hr of ischemia, followed by a linear increase for up to 48 hr. LDH levels, by contrast, increased linearly throughout the 48 hr of ischemia
Lamura et al., Biochem Pharmacol 1997 : The PLA2 is Ca2+ dependent and using EGTA regulated buffers cytosolic or particulate fraction activity was similar at both 10 microm or 10 mm Ca2+ concentrations
Murakami et al., FEBS Lett 1997 : Lysates of C57BL/6J derived BMMC contained a Ca2+ dependent PLA2 that was absorbed to a column conjugated with anti-type IIA sPLA2 antibody and had a similar molecular mass of 14 kDa, as assessed by immunoblotting
De Windt et al., J Mol Cell Cardiol 1997 : We report the cloning of a cDNA encoding a member of the Ca2+ dependent , low molecular mass phospholipase A2 (PLA2) present in rat heart
Bowman et al., Exp Parasitol 1997 : Though extracellular PLA2s are reported to be activated by millimolar Ca2+, ts-PLA2 was sensitive to submicromolar Ca2+ and was half-maximally activated by 3.5 microM Ca2+
Takuma et al., J Biochem 1997 : AACOCF3, a selective inhibitor of Ca2+ dependent cytosolic PLA2 , did not inhibit the exocytosis at least up to 30 microM
Adeagbo et al., J Vasc Res 1998 : We conclude that Ca2+ dependent PLA2 , rather than DAG lipase, generates the AA for the production of EDHF in the perfused rat mesenteric bed
Ma et al., Biochim Biophys Acta 1998 : Transfection of COS-7 cells with cPLA2 cDNA in an expression vector induced expression of Ca2+ dependent PLA2 activity and of a protein recognized by anti-cPLA2 antibody
Shiomi et al., Toxicon 1998 : The enzyme activity of both PLA2s was enhanced by Ca2+ but reduced by Cu2+ and Zn2+
Catalioto et al., Naunyn Schmiedebergs Arch Pharmacol 1998 : Activation of the PLA2 pathway may be linked to the opening of a voltage independent cation channel which activates a Ca2+ dependent PLA2
Eintracht et al., Biochem J 1998 : Here we report that Ca2+ influx in pathogenic Trypanosoma brucei can be regulated by phospholipase A2 (PLA2) and the subsequent release of arachidonic acid ( AA ) ... We identified in live trypanosomes PLA2 activity that was sensitive to OBAA and could be stimulated by Ca2+ , suggesting the presence of positive feedback control
Murthy et al., J Biol Chem 1998 : Differential regulation of phospholipase A2 (PLA2) dependent Ca2+ signaling in smooth muscle by cAMP- and cGMP dependent protein kinases