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Herman et al., Inflamm Res 2005 (Arthritis, Rheumatoid) : Apoptosis was measured by Annexin V test and FDA polarization ; and mitochondrial ROS generation by DHR123 probe and by NAC inhibition
Hossain et al., J Cell Biochem 2009 : We also found that a reducing agent dithiothreitol ( DTT ) and an anti-oxidant N-acetyl cysteine (NAC) inhibited BMTS mediated caspase-9 and -3 activation, ROS production and induction of Annexin V/propidium iodide double positive cells, suggesting the involvement of ROS in the apoptosis process
Gao et al., Anticancer Res 2013 (Ovarian Neoplasms) : NAC also blocked the inhibition of cell proliferation by CDDO-Me. Likewise, NAC prevented the CDDO-Me caused binding of fluorescein isothiocyanate ( FITC ) -tagged annexin V , cleavage of poly ADP-ribose polymerase-1 (PARP-1), procaspases-3, -8 and -9 and loss of mitochondrial membrane potential