◀ Back to RPTOR
RPTOR — ULK1
Pathways - manually collected, often from reviews:
-
NCI Pathway Database mTOR signaling pathway:
mTORC1 complex (MTOR-MLST8-RPTOR)
→
mTORC1/ULK1-2/ATG13/FIP200 complex (MTOR-MLST8-RPTOR-ULK1_ULK2-ATG13-RB1CC1)
(modification, collaborate)
Hosokawa et al., Mol Biol Cell 2009, Jung et al., Mol Biol Cell 2009, Ganley et al., J Biol Chem 2009
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database mTOR signaling pathway:
mTORC1 complex (MTOR-MLST8-RPTOR)
→
ULK1-2/ATG13/FIP200 complex (ULK1_ULK2-ATG13-RB1CC1)
(modification, collaborate)
Hosokawa et al., Mol Biol Cell 2009, Jung et al., Mol Biol Cell 2009, Ganley et al., J Biol Chem 2009
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database mTOR signaling pathway:
mTORC1/ULK1-2/ATG13/FIP200 complex (MTOR-MLST8-RPTOR-ULK1_ULK2-ATG13-RB1CC1)
→
ULK1-2/ATG13/FIP200 complex (ULK1_ULK2-ATG13-RB1CC1)
(modification, collaborate)
Hosokawa et al., Mol Biol Cell 2009, Jung et al., Mol Biol Cell 2009, Ganley et al., J Biol Chem 2009
Evidence: mutant phenotype, assay, physical interaction
-
WikiPathways Focal Adhesion-PI3K-Akt-mTOR-signaling pathway:
MLST8/MTOR/RPTOR
→
ULK1
(inhibition)
-
WikiPathways PI3K-AKT-mTOR signaling pathway and therapeutic opportunities:
Complex of RPTOR-MTOR
→
Complex of RB1CC1-ULK1-ATG13
(activation)
-
WikiPathways Target Of Rapamycin (TOR) Signaling:
Complex of RPTOR-MLST8-MTOR
→
ULK1
(inhibition)
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
Text-mined interactions from Literome
Jung et al., Mol Biol Cell 2009
:
ULK-Atg13-FIP200 complexes
mediate mTOR signaling to the autophagy machinery ... These findings demonstrate that the
ULK-Atg13-FIP200 complexes are direct targets of mTOR and important regulators of autophagy in
response to
mTOR signaling
Dunlop et al., Autophagy 2011
:
ULK1 inhibits
mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding ... Here, we demonstrate that
ULK1 induces multisite phosphorylation of
Raptor in vivo and in vitro ... Interestingly,
ULK1 overexpression also increases phosphorylation of Raptor Ser863 and the mTOR autophosphorylation site, Ser2481 in a
mTORC1 dependent manner ... Interestingly,
ULK1 overexpression also increases phosphorylation of Raptor Ser863 and the mTOR autophosphorylation site, Ser2481 in a
mTORC1 dependent manner ... We propose a new mechanism whereby
ULK1 contributes to
mTORC1 inhibition through hindrance of substrate docking to Raptor
Jung et al., Autophagy 2011
:
ULK1 inhibits the kinase activity of
mTORC1 and cell proliferation ... ( 1-3 ) Here, we investigated how ULK1 regulates mTORC1 signaling, and found that
ULK1 inhibits the kinase activity of
mTORC1 and cell proliferation ... Deficiency or knockdown of ULK1 or its homolog ULK2
enhanced mTORC1 signaling, cell proliferation rates and accumulation of cell mass, whereas overexpression of
ULK1 had the opposite effect ... In addition,
ULK1 was found to bind raptor, induce its phosphorylation, and
inhibit the kinase activity of
mTORC1 ... In addition,
ULK1 was found to bind raptor, induce its phosphorylation, and
inhibit the kinase activity of
mTORC1 ... These results demonstrate that
ULK1 negatively
regulates the kinase activity of
mTORC1 and cell proliferation in a manner independent of Atg5 and TSC2
Huang et al., J Biol Chem 2011
:
Knockdown of mTOR, but not
Raptor or Rictor,
reduced p-ULK1 at Ser ( 757 ) and enhanced chemotherapy induced autophagy that resulted in a similar cytoprotective effect as shown for AZD8055
Castets et al., Cell Metab 2013
:
In young TSCmKO mice, constitutive and starvation induced autophagy is blocked at the induction steps via
mTORC1 mediated inhibition of
Ulk1 , despite FoxO3 activation