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E2F1 — TOPBP1

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: Complex of E2F1-TOPBP1 → AKT1 (increases, E2F1/TOPBP1 Activity) Liu et al., EMBO J 2006*
  Evidence: We now show that the regulation of E2F1 by TopBP1 involves the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway, and is independent of pocket proteins. Akt phosphorylates TopBP1 in vitro and in vivo.... The Akt-dependent oligomerization is crucial for TopBP1 to interact with and repress E2F1.
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → TopBP1 (TOPBP1) (modification, collaborate) Liu et al., Mol Cell Biol 2003
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → E2F1/DP/TopBP1 complex (E2F1-TFDP2_TFDP1-TOPBP1) (modification, collaborate)
  Liu et al., Mol Cell Biol 2003
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database E2F transcription factor network: TopBP1 (TOPBP1) → E2F1/DP/TopBP1 complex (E2F1-TFDP2_TFDP1-TOPBP1) (modification, collaborate)
  Liu et al., Mol Cell Biol 2003
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database E2F transcription factor network: E2F1/DP complex (E2F1-TFDP2_TFDP1) → E2F1/DP/TopBP1 complex (E2F1-TFDP2_TFDP1-TOPBP1) (transcription, activates)
  Liu et al., Mol Cell Biol 2003, Komori et al., EMBO J 2005
  Evidence: mutant phenotype, reporter gene, physical interaction

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: TOPBP1 — E2F1 (physical association, affinity chromatography technology) Liu et al., EMBO J 2006*
• IRef Biogrid Interaction: TOPBP1 — E2F1 (physical association, affinity chromatography technology) Liu et al., Mol Cell Biol 2003
• IRef Biogrid Interaction: TOPBP1 — E2F1 (direct interaction, two hybrid) Liu et al., Mol Cell Biol 2003
• IRef Biogrid Interaction: TOPBP1 — E2F1 (direct interaction, pull down) Yu et al., Science 2003*
• IRef Biogrid Interaction: TOPBP1 — E2F1 (direct interaction, pull down) Liu et al., Mol Cell Biol 2003
• IRef Biogrid Interaction: TOPBP1 — E2F1 (physical association, affinity chromatography technology) Guo et al., J Biol Chem 2010*
• IRef Dip Interaction: TOPBP1 — E2F1 (physical association, two hybrid) Liu et al., Mol Cell Biol 2003
• IRef Dip Interaction: TOPBP1 — E2F1 (physical association, affinity chromatography technology) Liu et al., Mol Cell Biol 2003
• IRef Dip Interaction: TOPBP1 — E2F1 (physical association, fluorescence microscopy) Liu et al., Mol Cell Biol 2003
• IRef Dip Interaction: TOPBP1 — E2F1 (physical association, coimmunoprecipitation) Liu et al., Mol Cell Biol 2003
• IRef Hprd Interaction: TOPBP1 — E2F1 (in vitro) Liu et al., Mol Cell Biol 2003, Manke et al., Science 2003*, Liu et al., EMBO J 2006*
• IRef Hprd Interaction: TOPBP1 — E2F1 (in vivo) Liu et al., Mol Cell Biol 2003, Manke et al., Science 2003*, Liu et al., EMBO J 2006*
• IRef Intact Interaction: TOPBP1 — E2F1 (physical association, pull down) Manke et al., Science 2003*
• IRef Intact Interaction: TOPBP1 — E2F1 (physical association, anti tag coimmunoprecipitation) Liu et al., EMBO J 2006*
• IRef Intact Interaction: TOPBP1 — E2F1 (direct interaction, pull down) Liu et al., EMBO J 2006*
• IRef Intact Interaction: TOPBP1 — E2F1 (physical association, anti bait coimmunoprecipitation) Liu et al., EMBO J 2006*
• IRef Ophid Interaction: TOPBP1 — E2F1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Liu et al., Mol Cell Biol 2003 : Regulation of E2F1 by BRCT domain containing protein TopBP1 ... Through this interaction, TopBP1 repressed multiple activities of E2F1 , including transcriptional activity, induction of S-phase entry, and apoptosis ... Thus, the specific interaction between TopBP1 and E2F1 during DNA damage inhibits the known E2F1 activities but recruits E2F1 to a BRCA1 containing repair complex, suggesting a direct role of E2F1 in DNA damage checkpoint/repair at stalled replication forks
Liu et al., Genes Dev 2004 : Through its BRCT domain, TopBP1 interacts with and represses exclusively E2F1 but not other E2F factors ... TopBP1 recruits Brg1/Brm, a central component of the SWI/SNF chromatin remodeling complex, to E2F1-responsive promoters and represses the activities of E2F1 , but not E2F2 or E2F3 ... Interestingly, TopBP1 is induced by E2F and interacts with E2F1 during G1/S transition
Yoshida et al., Oncogene 2004 : In this study, we showed that the transcription of human MCM10 and TopBP1 is activated by transcription factors E2F1-3 , but not by factors E2F4-7 ... Analysis of various MCM10 and TopBP1 promoter constructs showed that an E2F-responsive sequence in the vicinity of the transcription initiation site is necessary for the E2F1 induced activation of MCM10 and TopBP1 gene transcription, which is further suppressed by pRb
Usskilat et al., Gene 2006 : These data indicate a cooperative regulation of the TopBP1 promoter by Egr-1 and E2F
Liu et al., EMBO J 2006 : We now show that the regulation of E2F1 by TopBP1 involves the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway, and is independent of pocket proteins