◀ Back to IRF6
IRF6 — JUN
Pathways - manually collected, often from reviews:
-
BioCarta the information processing pathway at the ifn beta enhancer:
IFN-beta nucleosome complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP-SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP/hSWI/SNF complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP-SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
IFN-beta nucleosome complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP-SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
→
TAFs/TF2A/TBP/TF2F/TF2B/TF2H/TF2E/RNA POL II complex (GTF2A1-NR3C1-GTF2F1-GTF2B-GTF3A-GTF2E1-POLR2A)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP/hSWI/SNF complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP-SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
→
TAFs/TF2A/TBP/TF2F/TF2B/TF2H/TF2E/RNA POL II complex (GTF2A1-NR3C1-GTF2F1-GTF2B-GTF3A-GTF2E1-POLR2A)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
ATF-2 (ATF2)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
HMG1 (HMGB1)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50 complex (RELA)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
→
IRF (IRF5/IRF1/IRF3/IRF6/IRF7/IRF4/IRF2)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
→
c-JUN (JUN)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
IRF (IRF5/IRF1/IRF3/IRF6/IRF7/IRF4/IRF2)
→
c-JUN (JUN)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
PCAF
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
PCAF
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF complex (ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1 complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF complex (ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP)
→
histone transacetylase (CREBBP)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF complex (ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
histone transacetylase (CREBBP)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF complex (ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
hSWI/SNF complex (SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
hSWI/SNF complex (SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP/hSWI/SNF complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP-SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
(modification, collaborate)
-
BioCarta the information processing pathway at the ifn beta enhancer:
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP)
→
RELA/p50/ATF-2/IRF/c-JUN/HMG1/PCAF/CBP/hSWI/SNF complex (RELA-ATF2-IRF5_IRF1_IRF3_IRF6_IRF7_IRF4_IRF2-JUN-HMGB1-PCAF-CREBBP-SMARCA4-SMARCD1-ARID1A-SMARCC1-SMARCC2-SMARCB1-SMARCE1-ACTB)
(modification, collaborate)
Text-mined interactions from Literome
Chen et al., Immunology 1999
:
LPS activation of nuclear factor-kappa B (NF-kappaB),
activator protein-1 (AP-1) and p38 MAPK was also inhibited by SB 203580
Imai et al., FEBS Lett 1999
:
Since recent studies showed that
c-Jun N-terminal kinase (JNK) , one of the mitogen activated protein kinases,
plays an important role in
LPS signalling, we focused here on the inhibitory action of TGF-beta1 on LPS stimulated JNK activity in mouse macrophages
Kim et al., Cancer Res 2000
(MAP Kinase Signaling System) :
Furthermore,
IRF7 was
activated by the
c-Jun NH2-terminal kinase (JNK) in response to DNA damaging agents
Steer et al., J Biol Chem 2000
:
LPS increased nuclear
c-Jun/ATF-2 , NF-kappaB ( 1 ) /Rel-A, and Rel-A/C-Rel transcription factor complexes, which bound specifically to oligonucleotide sequences from the -106 to -88 base pair ( bp ) region of the promoter
Tengku-Muhammad et al., Cytokine 2000
:
The
regulation of macrophage
activator protein-1 (AP-1) gene expression by
LPS and cytokines is of potentially crucial importance in the pathogenesis of several diseases
Simoncini et al., Circ Res 2000
:
Consistently, gel-shift assays showed that E ( 2 ) and Dex comparably inhibit LPS induced activation of NF-kappaB, whereas E ( 2 ) inhibited
LPS induced
activation of
AP-1 and GATA to a greater extent than Dex
Lakics et al., J Immunol 2000
:
Although the composition of NF-kappaB complexes detected by EMSA and supershift analysis in nuclear lysates derived from Bcl-xL transfectants and control cells was indistinguishable,
LPS induced inhibitory kappaBalpha degradation, as well as NF-kappaB binding and
AP-1 activation, were slightly decreased by ectopic expression of Bcl-xL
Jeon et al., Immunopharmacology 2000
:
Third, angelan induced strong NF-kappaB/Rel and slight AP-1 DNA binding, whereas
LPS potently
activated both NF-kappaB/Rel and
AP-1
Procyk et al., Blood 2000
:
The
activation of
Jun N-terminal kinases (JNK) by
LPS is herbimycin sensitive
Shen et al., J Immunol 2001
:
We demonstrate that
AP-1 ( Fos and Jun ),
induced transiently by CD40 ligand or
LPS , binds a DNA element in the mouse GL epsilon promoter
Hsu et al., J Immunol 2001
:
The nuclear translocation and DNA binding study revealed that ceramide can inhibit
LPS induced NF-kappaB and
AP-1 activation
Møller et al., Thromb Res 2001
:
Finally, both
LPS and AraLAM activated the `` early immediate genes '' through translocation of the transcription factor proteins NF-kappaB/Rel and
increasing the binding activity of
AP-1
Jones et al., J Leukoc Biol 2001
(MAP Kinase Signaling System) :
Stimulation of RAW 264.7 macrophages by
LPS , LAM, STF, and PIM rapidly
activated nuclear factor (NF)-kappaB,
activator protein-1 (AP-1) , and mitogen activated protein ( MAP ) kinases
García-Lora et al., Cancer Immunol Immunother 2001
:
Here we report that PSK
enhanced AP-1 and CRE binding activities, whereas IL-2 increased AP-1 and SP-1 and modified GAS/ISRE,
IRF-1 and STAT5
Supajatura et al., J Immunol 2001
(Acute Disease...) :
TLR4 mediated stimulation of mast cells by
LPS was
followed by activation of NF-kappaB but not by stress activated protein
kinase/c-Jun NH2-terminal kinase signaling
Matsumura et al., Life Sci 2001
:
However, activation of
activator protein-1 (AP-1) , which is also contained in the iNOS promoter, was not
enhanced by
LPS/IFN or inhibited by DEX
Yi et al., Int Immunol 2001
:
In addition, CpG DNA showed little or no enhancement of
LPS mediated
AP-1 activation
Simonin et al., Am J Physiol Cell Physiol 2002
:
Troglitazone had no effect on NF-kappa B activation and was shown to increase
LPS induced
AP-1 activation
Galdiero et al., Infect Immun 2002
(MAP Kinase Signaling System) :
PD-098059 pretreatment of cells decreases AP-1 and NF-kappaB activation by lipopolysaccharide (LPS) but not by porins, and SB203580 pretreatment of cells decreases mainly AP-1 and NF-kappaB activation by porins ; in contrast, forskolin pretreatment of cells does not affect
AP-1 and NF-kappaB
activation following either porin or
LPS stimulation
Suh et al., Toxicol Appl Pharmacol 2002
(Lymphoma) :
Electrophoretic mobility shift assays and chloramphenicol acetyl transferase reporter gene experiments demonstrated that
lipopolysaccharide (LPS) induced DNA binding and transcriptional activity of
AP-1 was markedly inhibited by TCDD at 24, 48, and 72 h after cellular activation of CH12.LX cells ... Concordant with this result, TCDD did not inhibit
LPS induced
AP-1 activity in BCL-1 B cells
Tsi et al., Mol Pharmacol 2002
:
ATA not only inhibited nuclear factor-kappaB (NF-kappaB) activation through impairment of the targeting and degradation of IkappaBs but also reduced
LPS induced
activator protein-1 (AP-1) activation
Hidding et al., Biochem Pharmacol 2002
:
Following LPS and other stimuli such as thrombin ( 10-50 unit/mL ), the activation of JNKs went along with the N-terminal phosphorylation of c-Jun which persisted for at least 8 hr. Indirect inhibition of JNK by CEP-11004 ( 0.5-2 microM ), an inhibitor of mixed-lineage kinases (MLK), reduced the
LPS induced phosphorylation of both, JNK and
c-Jun , by around 50 %, and attentuated the LPS induced the alterations in microglial morphology
Chano et al., Eur J Immunol 2002
:
Electrophoretic mobility shift assays and luciferase reporter constructs revealed that
LPS induced
AP-1 transcriptional activity was normal in DN PKC-alpha overexpressing RAW 264.7 cells
Pinteaux et al., J Neurochem 2002
:
Bacterial
lipopolysaccharide (LPS) caused increased expression of IL-1RI, IL-1RII and IL-1RAcP mRNAs, induced the release of IL-1beta, IL-6 and prostaglandin-E2 ( PGE2 ), and
activated nuclear factor kappaB (NF-kappaB) and the mitogen activated protein kinases ( MAPKs ) p38, and extracellular signal regulated protein kinase ( ERK1/2 ), but not
c-Jun N-terminal kinase (JNK) in microglial cultures
Ferlito et al., J Endotoxin Res 2002
:
Pretreatment of THP-1 cells with PTx attenuated
LPS induced
activation of
c-Jun-N-terminal kinase (JNK) and p38 kinase, and production of tumor necrosis factor-alpha (TN-alpha) and thromboxane B2 ( TXB2 )
Paik et al., Hepatology 2003
(Liver Cirrhosis) :
LPS also
activates c-Jun N-terminal kinase (JNK) , as assessed by in vitro kinase assays
MacKenzie et al., Br J Pharmacol 2003
(MAP Kinase Signaling System) :
2. In addition, PDTC potentiated
LPS stimulated
c-Jun N-terminal kinase (JNK) , p38 mitogen activated protein kinase ( MAP kinase ) and MAP kinase activated protein kinase-2 activity ( the downstream target of p38 MAP kinase )
Cuschieri et al., Shock 2003
(MAP Kinase Signaling System...) :
LPS stimulation
led to mitogen activated protein kinase activation and translocation of
activator protein-1 (AP-1) and nuclear factor (NF)-kappaB
Lenert et al., Antisense Nucleic Acid Drug Dev 2003
:
In a cell line transfected with an AP-1-beta-galactosidase reporter construct, CpG ODN induced AP-1 transcriptional activity was prevented by inhibitory ODN, but
lipopolysaccharide (LPS) induced
AP-1 activity was not
Napolitani et al., Eur J Immunol 2003
:
Activation of src-family tyrosine kinases by
LPS regulates cytokine production in dendritic cells by controlling
AP-1 formation
Kang et al., Biochem Biophys Res Commun 2004
:
Additionally, specific inhibitors of SAPK/JNK and p38 MAP kinase, SP600125 and SB203580, also suppressed
LPS induced increase in IL-1beta gene expression and
AP-1 DNA binding
Kang et al., J Pharmacol Sci 2004
:
Furthermore, curcumin markedly inhibited
LPS induced nuclear factor kappaB (NF-kappaB) and
activator protein 1 (AP-1) DNA bindings
Cuschieri et al., Cell Immunol 2004
:
Subsequently,
LPS induced the degradation of IkappaB, and the nuclear activation of NF-kappaB and
AP-1 ... Proteasome inhibition, also, led to increased
LPS induced
AP-1 activation, and attenuated LPS induced IkappaB degradation resulting in abolished NF-kappaB activation
Krappmann et al., Mol Cell Biol 2004
:
Intriguingly, the
induction of
AP-1 activity by
LPS in precursor B cells and primary dendritic cells fully depends on the IKK/NF-kappaB pathway, which promotes expression of several AP-1 family members, including JunB, JunD, and B-ATF ... Thus, our data illustrate that NF-kappaB orchestrates immediate-early effects of
LPS signaling and
controls secondary
AP-1 activation to mount an appropriate biological response
Chambers et al., Clin Exp Immunol 2004
(HIV Infections) :
HIV infection of THP-1 myeloid cells resulted in decreased
LPS induced nuclear factor binding to the NF-kappaB,
AP-1 , and Sp1 sites of the IL-12 p40 promoter
Li et al., Zhonghua Shao Shang Za Zhi 2004
(Acute Lung Injury) :
To investigate the changes in plasma level of interleukin-13 (IL-13) and the changes in the pulmonary IL-13 mRNA content and the pulmonary
activator protein-1 (AP-1) activity of the rats inflicted with acute lung injury ( ALI )
induced by
lipopolysaccharide (LPS) , so as to explore the relationship between IL-13 expression and AP-1 activity
Cuschieri et al., J Surg Res 2004
:
Subsequently,
LPS induced the activation of NF-kappaB and
AP-1
Wu et al., Mol Cell Biol 2004
:
Inhibition of SPK by the pharmacological inhibitor N, N-dimethylsphingosine ( DMS ) or SPK1-specific siRNA blocked LPS stimulation of extracellular signal regulated kinase 1/2 and p38 but enhanced
LPS induced
c-Jun N-terminal kinase activation
Laan et al., J Immunol 2004
:
In addition, the activity of
LPS induced transcription factor
AP-1 , but not NF-kappaB, was down-regulated by CSE
Zhao et al., J Nutr Biochem 2005
:
In addition, EPA pretreatment decreased
LPS induced
c-Jun phosphorylation and c-Jun N-terminal kinase (JNK) activation
Pandey et al., Int Immunopharmacol 2005
:
B. diffusa hexane, chloroform and ethanol extracts, and two pure compounds Bd-I ( eupalitin-3-O-beta-D-galactopyranoside ) and Bd-II ( eupalitin ) were evaluated in vitro for their effect on T cell mitogen ( phytohemagglutinin ; PHA ) stimulated proliferation of human peripheral blood mononuclear cell ( PBMC ), mixed lymphocyte culture, lipopolysaccharide (LPS) stimulated nitric oxide production by RAW 264.7, PHA and
LPS induced IL-2 and TNF-alpha production, in human PBMCs, superoxide production in neutrophils, human natural killer ( NK ) cell cytotoxicity and nuclear translocation of nuclear factor- ( kappa ) B and
AP-1 in PHA stimulated PBMCs
Waetzig et al., Glia 2005
(Encephalitis...) :
Stimulation with
LPS increased the total amount of nuclear JNKs and the phosphorylation of the transcription factor
c-Jun ... The function of JNKs in LPS triggered cellular reactions was investigated using SP600125 ( 0.5-5 microM ), a direct inhibitor of JNKs. Inhibition of JNKs reduced the LPS induced metabolic activity and induction of the
AP-1 target genes cyclooxygenase-2 (Cox-2), TNF-alpha, monocyte chemoattractant protein-1 ( MCP-1 ), and interleukin-6 (IL-6) in
response to
LPS , while ERK1/2 and p38 alpha had a more pronounced effect on LPS induced cellular enlargement than JNKs
Stollenwerk et al., Atherosclerosis 2005
(Arteriosclerosis...) :
VLDL did not activate transcription factors NF-kappaB and PPAR-gamma, but it activated AP-1 at least as potently as LPS, and potentiated
LPS induced activation of
AP-1
Mishra et al., J Biol Chem 2005
(Calcium Signaling) :
We previously demonstrated that the
c-Jun N-terminal kinase (JNK) , a mitogen activated protein kinase ( MAPK ), differentially
regulated LPS- but not TNF-alpha induced CD44 expression in monocytic cells
Suuronen et al., Inflamm Res 2005
(Inflammation) :
We also observed that the raloxifene induced protection in N9 microglia was connected to a decline of
LPS induced DNA binding activity of
AP-1 but not that of NF-kappaB transcription factors
Rak Min et al., Life Sci 2005
:
As a mechanism of the anti-inflammatory action shown by CP compound, suppression of
LPS induced activation of both nuclear factor-kappaB (NF-kappaB) and
activator protein-1 (AP-1) has been documented ... Finally, CP compound could provide an invaluable tool to investigate
LPS induced NF-kappaB and
AP-1 activation, in addition to its therapeutic potential in NO- and IL-6 associated inflammatory diseases
Sasaki et al., Am J Physiol Regul Integr Comp Physiol 2005
(Dehydration...) :
Both hepatic and splenic
AP-1 expressions were
enhanced by
LPS
Yamada et al., J Urol 2005
(Urinary Bladder Neoplasms) :
Although LPS and IFN-gamma alone marginally activated nuclear factor (NF)-kappaB but not AP-1,
LPS plus IFN-gamma
augmented NF-kappaB and
AP-1
Uto et al., Biochem Pharmacol 2005
:
6-MITC suppressed
LPS induced
c-Jun phosphorylation, but did not inhibit IkappaB-alpha degradation
Chen et al., Eur J Pharmacol 2005
:
LPS induced IkappaB kinase (IKK), nuclear factor-kappaB (NF-kappaB) and
activating protein-1 (AP-1) activation , and IFN-gamma induced NF-kappaB, signal transducer and activator of transcription-1 ( STAT1 ) and interferon regulatory factor-1 (IRF-1) activation were reduced by quercetin
Sheu et al., Food Chem Toxicol 2006
(Glomerulonephritis) :
Electrophoretic mobility shift assay experiments demonstrated that CLA ( 100 microM ) dramatically reduced activation of nuclear factor-kappaB (NF-kappaB),
activator protein-1 (AP-1) and cAMP response element binding protein ( CREB )
induced by
LPS/IFN-gamma
Wang et al., Am J Respir Cell Mol Biol 2006
(Inflammation) :
LPS induced
activation of NF-kappaB and
AP-1 determined by electrophoretic mobility shift assay were significantly reduced by overexpressing caveolin-1 in RAW264.7 cells
Lendemans et al., J Endotoxin Res 2006
:
LPS induced
AP-1 binding to DNA was also enhanced in GM-CSF-pre incubated cells ... It is concluded that a tyrosine kinase of the GM-CSF triggered ERK1/2 pathway augments the
LPS induced NF-kappaB and
AP-1 activation
Lahti et al., BMC pharmacology 2006
:
Treatment with SB220025 increased also
LPS induced
c-Jun N-terminal kinase (JNK) activity
Wang et al., J Biol Chem 2006
:
Ablation of jnk2 did not inhibit
GalN/LPS induced
c-Jun kinase activity, although activity was completely blocked in jnk1-/- mice
Bae et al., Planta Med 2006
:
Electrophoretic mobility shift assays revealed that ginsenoside Rh2 significantly inhibited the
LPS/IFN-gamma induced
AP-1 DNA binding activity, while it enhanced the protein binding to CRE sequences
Jin et al., Planta Med 2006
:
Polyozellin suppressed the activation of both
LPS induced NF-kappaB and the stress activated protein kinase ( SAPK )
/c-Jun N-terminal kinase (JNK) , but had no effect on the extracellular signal regulated kinase ( ERK ) or p38
Patel et al., Biochem Biophys Res Commun 2006
:
LPS stimulated both
AP-1 ( c-Fos, c-Jun ) and NF-kappaB ( p50 and p65 ) activation, but only inhibition of AP-1 attenuated LPS induced CXCR6 expression ... Using dominant negative expression vectors and siRNA interference, we demonstrate that
LPS induces
AP-1 activation via MyD88, TRAF6, ERK1/2, and JNK signaling pathways
Hirasawa et al., Life Sci 2006
:
The JNK inhibitor SP600125 ( 3-30 microM ) suppressed the
LPS induced phosphorylation of
c-Jun , and inhibited the LPS induced production of histamine and expression of the HDC mRNA and 74-kDa protein in a concentration dependent manner
Liedtke et al., J Hepatol 2006
:
Ablation of jnk2 did not inhibit
GalN/LPS induced
c-Jun kinase activity, although activity was completely blocked in jnk1-/- mice
Uto et al., Oncol Rep 2007
:
LPS induced activation of all of these factors and 6-MITC
suppressed LPS induced activation of
AP-1 , C/EBPdelta, CREB, but not NF-kappaB
Ramana et al., Cytokine 2006
:
The
LPS induced DNA binding activity of NF-kappaB and
AP1 were significantly inhibited by AR inhibitors, and this effect was mediated through the inhibition of phosphorylation of IkappaB-alpha, IKK alpha/beta and PKC
Lin et al., Biochem Pharmacol 2007
:
LPS induced
activator protein-1 (AP-1) DNA binding, AP-1 dependent reporter gene activity and c-Jun nuclear translocation were all markedly inhibited by CYL-4d with similar efficacy, whereas CYL-4d produced a weak inhibition of nuclear factor-kappaB (NF-kappaB) DNA binding, NF-kappaB dependent reporter gene activity and p65 nuclear translocation without affecting inhibitory factor-kappa B alpha ( I kappa B alpha ) degradation
Pokharel et al., Biol Pharm Bull 2007
:
The lignan suppressed the reporter gene activity and the
LPS induced reporter activations of nuclear factor-kappaB (NF-kappaB) and
activator protein-1 (AP-1) were also significantly blocked by 4-hydroxykobusin
Gafencu et al., J Biol Chem 2007
(Inflammation) :
In addition to NF-kappaB activation,
LPS also
activated c-Jun via its phosphorylation by JNK
Luyendyk et al., Arterioscler Thromb Vasc Biol 2007
:
These compounds also reduced
LPS induction of nuclear
AP-1 and expression of Egr-1 without affecting nuclear translocation of NF-kappaB
Huang et al., Int Immunopharmacol 2007
:
Activation of JNKs ( not ERKs ) via phosphorylation induction, and an increase in
c-Jun ( not c-Fos ) protein expression were
involved in
LPS- and LTA treated RAW264.7 cells, and those events were blocked by Wog, but not N-Wog, addition
Liu et al., J Ethnopharmacol 2007
(Edema...) :
To clarify the mechanisms involved, methanol extracts from fruiting body were found to inhibit the phosphorylation of extracellular signal regulated protein kinases ( ERK ),
c-Jun NH2-terminal protein kinases (JNK) and signal transducer and activator of transcription-1 ( STAT-1 )
induced by
LPS/IFN-gamma
Muroi et al., Glycobiology 2007
:
Among the LPS induced signaling pathways that induce production of TNF-alpha, the activation of
c-Jun N-terminal kinase (JNK) was greatly
enhanced by
LPS and C-Man-WSPW, and the production of TNF-alpha was suppressed by an inhibitor for JNK
El Gazzar et al., Inflamm Res 2007
:
Chromatin immunoprecipitation revealed that GATA,
AP-1 and NF-AT binding to IL-5 promoter was
induced by
LPS stimulation and that TQ inhibited GATA binding at the IL-5 promoter but did not affect AP-1 and NF-AT binding
Smolinska et al., Mol Immunol 2008
:
In contrast, PP2 did inhibit
AP-1 nuclear accumulation in
response to
LPS
Chen et al., Life Sci 2007
(Inflammation) :
In addition, luteolin significantly inhibited the
LPS induced DNA binding activity of
activating protein-1 (AP-1)
Cho et al., J Nutr 2008
(Inflammation) :
Furthermore, DIM decreased
LPS induced transcriptional activity of activator protein (AP)-1,
AP-1 DNA binding activity, and phosphorylation of stress activated protein kinase/Jun-N-terminal kinase and c-Jun
Tanaka et al., Neurosci Lett 2008
:
In primary glial cultures,
LPS increased the phosphorylation levels of
c-Jun amino-terminal kinase 1 (JNK1) and p38 mitogen activated protein kinase ( MAPK ) ; in primary microglial cultures, it enhanced phosphorylation of extracellular signal regulated kinase ( Erk )
Miyoshi et al., Eur J Neurosci 2008
:
ANG II application enhanced the
LPS induced increases in IL-1 and nitrite concentrations, as well as the LPS induced morphological changes and
AP-1 activation, and these enhancements were inhibited by losartan ( 10 ( -5 ) M )
Wu et al., Toxicol Appl Pharmacol 2008
(Translocation, Genetic) :
LPS stimulated phosphorylation of c-Jun N-terminal kinase and translocation of
c-Jun and c-Fos from the cytoplasm to nuclei ... However, administration of ketamine significantly decreased
LPS induced activation of c-Jun N-terminal kinase and translocation of
c-Jun and c-Fos ... Administration of ketamine significantly ameliorated
LPS induced DNA binding activity of
activator protein-1
Ortiz-Lazareno et al., Immunology 2008
:
Proteasome inhibition also led to an increase in
LPS+PMA induced
AP-1 activation and the attenuation of LPS+PMA induced IkappaB degradation, resulting in the abolition of NF-kappaB activation
Iwasaki et al., Endocr J 2008
:
LPS also stimulated the expression of c-Fos gene and protein, and
AP1- , but not NF-kappaB-,
mediated transcription
Kang et al., Phytother Res 2008
(Edema) :
Further study demonstrated that
LPS induced DNA binding of
AP-1 and phosphorylation of c-jun N-terminal kinase ( JNK ) were inhibited by MK treatment in RAW 264.7 cells, whereas phosphorylation of p38 mitogen activated protein kinase was unaffected
Chen et al., Eur J Pharmacol 2008
(MAP Kinase Signaling System) :
Further investigation of the mechanisms revealed that 8-prenylkaempferol inhibited
LPS induced
c-Jun phosphorylation ( a major component of activator protein-1, AP-1 ), but did not attenuate IkB-alpha degradation nor NF-kappaB nuclear translocation ... On the other hand,
LPS induced
c-Jun phosphorylation was attenuated in the presence of SP600125
Sundararaj et al., J Leukoc Biol 2008
:
Moreover, we showed that simvastatin inhibited
LPS stimulated nuclear
AP-1 , but not NF-kappaB activity, and the inhibition was reversed by addition of GGPP
Yoshizawa et al., J Immunol 2008
(Arthritis, Rheumatoid) :
siRNA knockdown studies showed that TNF, PGN, and
LPS induced JNK and
c-Jun phosphorylation are MKK7 dependent, while poly ( I-C ) responses require both MKK4 and MKK7
Cho et al., Am J Physiol Endocrinol Metab 2008
(Body Weight...) :
In C57BL/6 mice, compound A reduced
LPS mediated increases in both plasma cytokine levels and phosphorylated
c-Jun in adipose tissue
Cho et al., Mol Endocrinol 2009
:
Activated GR inhibited
LPS induced
activator protein 1 activity, which in turn decreased activating transcription factor 2/c-Jun phosphorylation
Pang et al., Acta Pharmacol Sin 2009
(Inflammation...) :
The
LPS induced increase in the DNA binding activity of NF-kappaB and
activator protein 1 (AP-1) , the nuclear translocation of NF-kappaB p65, the degradation of the inhibitory kappaBalpha protein ( IkappaBalpha ) and the phosphorylation of IkappaBalpha, IkappaB kinase (IKK) alpha/beta, c-Jun NH ( 2 ) -terminal kinase ( JNK ) and p38 MAPKs were all suppressed by FLZ
Bumrungpert et al., J Nutr 2009
(Inflammation...) :
alpha- and gamma-MG also attenuated
LPS activation of c-Jun and
activator protein (AP)-1 activity
Park et al., Phytother Res 2010
:
Further study demonstrated that glabridin inhibited
LPS induced DNA binding activity of NF-kappaB and
AP-1 in BV-2 cells
Wu et al., Br J Pharmacol 2009
:
Effects of tripterine were investigated on endothelial barrier function, inducible nitric oxide synthase (iNOS) expression, nicotinamide adenine dinucleotide phasphate ( NADPH ) oxidase activity, 3-nitrotyrosine formation, protein phosphatase type 2A (PP2A) activity,
activation of extracellular regulated kinase (ERK),
c-Jun terminal kinase (JNK) and Janus kinase ( Jak2 ), and degradation of IkappaB in microvascular endothelial cells exposed to pro-inflammatory stimulus [
lipopolysaccharide (LPS) + interferon gamma (IFNgamma) ] and on vascular permeability in air pouches of mice injected with LPS + IFNgamma
Murakami et al., Anticancer Res 2009
:
We also investigated tThe inhibitory effects of these compounds on
lipopolysaccharide (LPS) stimulated cyclooxygenase-2 (COX-2) mRNA and protein expression and on binding of
activator-protein-1 (AP-1) and nuclear factor kappa-B (NF-kappaB) to their respective consensus sequences were also investigated in RAW 264.7 cells ... 2,2'-Biphenol, but not 4,4'-biphenol, showed inhibitory effects on
LPS stimulated COX-2 expression and on
AP-1 and NF-kappaB binding to their consensus sequences at 1-10 muM
Folco et al., J Biol Chem 2009
(Inflammation...) :
We demonstrate that pretreatment of these cells with adiponectin inhibits phosphorylation of nuclear factor kappaB inhibitor ( IkappaB ),
c-Jun N-terminal kinase (JNK) , and p38 mitogen activated protein kinase ( MAPK ),
induced by either
lipopolysaccharide (LPS) or tumor necrosis factor (TNF) alpha, as well as STAT3 phosphorylation induced by interleukin-6 (IL6)
Chuang et al., Toxicol Lett 2009
:
LPS selectively
enhanced translocation of transcription factor
c-Jun from the cytoplasm to nuclei, but not nuclear factor kappa-B ... In parallel, the DNA binding activity of
AP-1 was
increased by
LPS ... Taken together, this study has shown that
LPS selectively
induces SP-A gene expression possibly through TLR2 mediated activation of
c-Jun in human alveolar epithelial A549 cells
Weng et al., J Biomed Sci 2009
(Liver Cirrhosis, Biliary...) :
In vitro, Arm ( 1-10 microM ) concentration-dependently attenuated TNF-alpha- and
LPS stimulated alpha-SMA protein expression and
AP-1 activation by HSC-T6 cells without adverse cytotoxicity
Yadav et al., J Immunol 2009
(Asthma) :
We observed that pharmacological inhibition or genetic ablation of AR by small interfering RNA prevented TNF-alpha- as well as
LPS induced apoptosis ; reactive oxygen species generation ; synthesis of inflammatory markers IL-6, IL-8, and PGE ( 2 ) ; and
activation of NF-kappaB and
AP-1 in small airway epithelial cells
Cha et al., Immunol Invest 2009
(Inflammation) :
In addition, the essential oil suppressed the
LPS stimulated activation of mitogen activated protein kinases ( MAPKs ) as well as the
AP-1 DNA binding activity
Chang et al., Inflammation 2010
(Inflammation) :
Moreover,
LPS induced nuclear factor-kappaB (NF-kappaB) and
activator protein-1 (AP-1) nuclear protein-DNA binding affinity and reporter gene activity were significantly decreased by LA and GLA
Brandenburg et al., Inflamm Res 2010
(Inflammation) :
SF inhibits LPS stimulated ERK1/2 and JNK phosphorylation and thereby inhibits the
LPS induced activation of NF-kappaB- and
activator protein-1 (AP-1)
Overman et al., Int J Obes Relat Metab Disord 2010
(Inflammation...) :
We investigated the effect of GPE pretreatment on
LPS mediated
activation of mitogen activated protein kinases ( MAPKs ), nuclear factor kappa B (NF-kappaB) and
activator protein-1 (AP-1) , and induction of inflammatory genes in human MPhis ( that is, differentiated U937 cells ) ... Grape powder extract also attenuated
LPS activation of MAPKs, NF-kappaB and
AP-1 ( c-Jun ), as evidenced by decreased ( 1 ) phosphorylation of c-Jun NH ( 2 ) -terminal kinase ( JNK ) and p38 ; ( 2 ) degradation of IkappaBalpha and activation of an NF-kappaB reporter construct ; and ( 3 ) phosphorylation of c-Jun and Elk-1
Hwang et al., Int Immunopharmacol 2010
:
DSHP-U suppressed
LPS induced NO production and iNOS expression at a concentration of 50 microM and inhibited LPS induced phosphorylation of extracellular signal regulated kinase ( ERK ),
c-Jun N-terminal kinase (JNK) , and p38 kinase
Lee et al., Eur J Pharmacol 2010
(MAP Kinase Signaling System) :
Moreover, eupatolide significantly suppressed the
LPS induced expression of nuclear factor-kappa B (NF-kappaB) and
activator protein-1 (AP-1) reporter genes
Yoshioka et al., J Pharmacol Sci 2010
:
Furthermore, the effects of
LPS on iNOS and NO production were inhibited by the c-Jun N-terminal kinase (JNK) inhibitor SP600125, and LPS induced phosphorylation of JNK and
c-Jun was
inhibited by NOC18 and DBcGMP
Wiesner et al., Circ Res 2010
(Atherosclerosis...) :
In a nuclear factor-DNA binding assay, the cooperative
effect of mmLDL and
LPS costimulation on
c-Jun and c-Fos DNA binding, but not on p65 or p50, was dependent on mmLDL induced activation of extracellular signal regulated kinase ( ERK ) 1/2
Jung et al., Int Immunopharmacol 2010
:
Inhibition of these inflammatory mediators appears to be at the transcriptional level, since diosgenin decreased
LPS/IFN-gamma induced NF-kappaB and
AP-1 activity
Lu et al., Journal of neuroinflammation 2010
(Inflammation) :
Resveratrol inhibited
LPS induced NF-kappaB activation in both cell types, but
inhibited AP-1 activation only in microglia
Li et al., Phytochemistry 2010
:
Compounds 1, 5-7, 9-10, and 13 showed anti-inflammatory potency to suppress expression of NF-?B and
AP-1 targeted genes including TNF-a and IL-6
induced by
lipopolysaccharide (LPS) in mouse macrophages Raw 264.7 cells
Chiou et al., Biochem Pharmacol 2011
:
In conclusion, TH-4-PX inhibited
LPS induced NF-?B and
AP-1 activations by interfering with the posttranslational modification ( phosphorylation and/or ubiquitinylation ) of IRAK-1 in the cell membrane to impede TAK1 mediated activation of IKK and MAPKs signal transduction
Jedinak et al., Nutrition journal 2011
(Inflammation) :
OMC also inhibited
LPS dependent DNA binding activity of
AP-1 and NF-?B in RAW264.7 cells
Zhang et al., Inflamm Res 2011
(Inflammation) :
Meanwhile, the activation of p38 MAPK and
c-Jun N-terminal kinases (JNK) induced by
LPS were decreased by MHNA
Wu et al., Biochem Biophys Res Commun 2011
(Inflammation) :
Further, we analyzed the
effect of
LPS on the DNA binding activity of NF-?B, C/EBP and
AP-1 transcription factors in liver and lung from WT and AhR ( -/- ) mice
Vaz et al., Am J Respir Cell Mol Biol 2012
(Acute Lung Injury...) :
LPS induced mortality, lung injury, inflammation, cytokine measurements, and
AP-1 and NF-?B activities were then assessed in these mice
Tsai et al., J Investig Med 2011
(Inflammation...) :
Its suppressive effect on TNF-a, I-309, and IP-10 may, at least in part, involve the down-regulation of
LPS induced
MKK4-JNK-c-Jun expression
Küper et al., American journal of physiology. Renal physiology 2012
:
Inhibition of p38 or ERK1/2 had no significant effect on
LPS induced NF-?B activation, nor on
activator protein 1- , cAMP response element-, or serum response element-driven reporter constructs
Albanito et al., Glia 2011
:
Bergmann glia derived cells expressed toll-like receptor 4 and treatment with bacterial
lipopolysaccharide (LPS) induced
c-Jun phosphorylation at serine 63, a hallmark of c-Jun transactivation, exclusively in BG cells ... Moreover,
LPS induced IL-1ß expression and inhibition of
c-Jun N-terminal kinase (JNK) activity abolished both c-Jun phosphorylation and the increase of IL-1ß mRNA
Krifka et al., Biomaterials 2012
:
LPS induced expression of activated transcription factors
c-Jun , ATF-2, ATF-3 and phospho-Elk1 was decreased in cells co-treated with TEGDMA
Finney et al., Intensive Care Med 2012
:
LPS and LTA
activated NF-?B and
AP-1 in J774 cells
Jin et al., Clin Immunol 2012
:
Furthermore, our studies showed that while
LPS activated
AP-1 and NF?B in U937 cells, it only activated NF?B in fibroblasts
Liu et al., Chinese medicine 2012
:
This study demonstrates that a-MG attenuates
LPS mediated
activation of MAPK, STAT1, c-Fos,
c-Jun and EIK-1, inhibiting TNF-a and IL-4 production in U937 cells
Byun et al., Biochem Biophys Res Commun 2012
(Inflammation) :
In addition, EGCG treated DCs inhibited
lipopolysaccharide (LPS) induced production of pro-inflammatory cytokines ( tumor necrosis factor [TNF ] -a, interleukin [ IL]-1ß, and IL-6 ) and
activation of mitogen activated protein kinases ( MAPKs ), e.g., extracellular signal regulated kinase 1/2 ( ERK1/2 ), p38,
c-Jun N-terminal kinase (JNK) , and nuclear factor ?B ( NF-?B ) p65 translocation through 67LR
Bang et al., J Ethnopharmacol 2012
(MAP Kinase Signaling System) :
Furthermore, AJ suppressed
LPS induced NF-?B
activation , degradation of I?B-a, phosphorylation of extracellular signal regulated kinase ( ERK ),
c-Jun N-terminal kinase (JNK) and p38
Kwon et al., Eur J Pharmacol 2012
(Inflammation) :
LPS induced expression levels of JunB,
c-Jun and c-Fos were also decreased by BCA treatment ... Moreover, the
LPS induced DNA binding activity of
AP-1 was markedly inhibited by BCA
Khan et al., J Ethnopharmacol 2013
(Edema...) :
Capillarisin also exhibited a promising inhibitory effect on the
LPS induced NF-?B and
AP-1 DNA binding activity based on an electrophoretic mobility shift assay
Wang et al., Experimental biology and medicine (Maywood, N.J.) 2012
(Inflammation) :
LPS also
activated mitogen acticated protein kinase ( MAPKs ),
c-Jun and NF-?B, and pro-inflammatory genes
Frei et al., J Allergy Clin Immunol 2013
(Inflammation) :
Histamine suppressed MDDC chemokine and proinflammatory cytokine secretion, nuclear factor ?B and
activator protein 1 activation, mitogen activated protein kinase phosphorylation, and TH1 polarization of naive lymphocytes, whereas IL-10 secretion was enhanced in
response to
LPS and Pam3Cys
Lee et al., J Ethnopharmacol 2013
:
HP decreased LPS induced microglial cell death by 24 % and inhibited
LPS induced activation of
c-Jun N-terminal kinase (JNK) by 23 % and p42/44MAP kinase ( ERK ) by 34 % treatment of LPS
Xu et al., CNS Neurosci Ther 2013
:
In addition, according to the study of related signaling pathways, RvD1 attenuated
LPS induced microglia NF-?B activation, MAPK phosphorylation, and
activator protein-1 transcriptional activity
Zhu et al., Journal of biomedical research 2010
:
LPS stimulated
JNK/AP-1 activation, which was inhibited by IL-13 in a dose dependent manner
Xue et al., Placenta 2013
:
Our study further proved that miR-155* targeted PTEN 3'-untranslated region ( 3'UTR ) increased IRAKM and NKIRAS1 expression by competing for miR-155* binding, thereby suppressing
AP-1/NF-?B activation
induced by
LPS
Kwon et al., Food Chem Toxicol 2013
:
Flavokawain A significantly inhibited
LPS induced activation of NF-?B and
AP-1 signaling pathways
Oh et al., Molecules (Basel, Switzerland) 2013
(Inflammation...) :
The extract also had no effect on
LPS stimulated p38 MAPK, JNK, and
c-Jun phosphorylation, whereas ERK1/2 phosphorylation was strongly inhibited by BOWE
Tran-Thi et al., Hepatology 1995
:
The results indicate a direct participation of NF-kappa B in the regulation of TNF-alpha synthesis and a differential
effect of
LPS on NF-kappa B and
AP-1 , respectively
Dokter et al., Blood 1993
:
Finally, using electrophoretic mobility shift assays, evidence was obtained that IL-4 inhibits
LPS induced expression of
AP-1 protein
Pierce et al., J Clin Invest 1996
:
These data indicate that, in contrast to most LPS effects,
AP-1 , but not nuclear factor-kappaB,
mediates LPS induction of collagenase transcription in macrophagelike cells
de Wit et al., Exp Hematol 1996
:
Not only was this effect observed at the mRNA level, but activator protein-1 (AP-1) DNA binding capacity was affected as well, A strong reduction was observed in the
LPS induced DNA binding activity of
AP-1 in the presence of IFN-gamma
Groupp et al., J Biol Chem 1996
:
These data suggest that
LPS stimulation of THP-1 cells
activates binding of
c-Jun , Ets, and Egr-1 to the TF promoter and implicates these factors in the transcriptional activation of TF mRNA synthesis
Sanghera et al., J Immunol 1996
:
Bacterial
LPS stimulation of murine macrophages
leads to increased tyrosine phosphorylation and activation of the 42- and 44-kDa mitogen activated protein kinases ( MAPK ) and the activation of stress activated protein kinases ( SAPK )
/c-Jun N-terminal kinase (JNK) and p38, related to the high osmolarity glycerol protein kinase in Saccharomyces cerevisiae ( HOG1 )
Dokter et al., Leukemia 1996
:
In electrophoretic mobility shift assays ( EMSAs ) we showed that IL-10 and IL-4 inhibited
LPS induced
AP-1 binding activity ... Downregulation of
LPS induced
AP-1 was accompanied, and thus possibly explained, by a reduced expression at mRNA level of the two major components of the AP-1 complex, namely c-fos and c-jun as determined by Northern experiments
Tanaka et al., Biochem Biophys Res Commun 1997
(Astrocytoma) :
Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise
LPS induced activation of both NF kappa B and
AP-1 was enhanced by BSO and inhibited by NAC
Yao et al., J Biol Chem 1997
:
LPS stimulation
increased the binding of
c-Jun containing complexes
Camhi et al., Am J Respir Cell Mol Biol 1998
:
Mutational analysis of the AB1 enhancer and electrophoretic-mobility-shift assays of nuclear extracts from LPS treated cells further demonstrated that the transcription factor
activator protein-1 (AP-1) is
critical for AB1 mediated HO-1 gene activation by
LPS
Sugita et al., Inflammation 1998
:
These results indicate that P. gingivalis
LPS activates NF-kappa B and
AP-1 in both serum dependent and -independent manners, followed by increased IL-8 transcription in neutrophils, and suggested a role for P. gingivalis LPS in IL-8 synthesis by neutrophils in inflamed gingiva and GCF
Greenberg et al., Alcohol 1998
:
ETOH does not directly inhibit NFkappaB or
AP-1 , in vivo, but rather
inhibits LPS induced activation of the MEKK/MAP kinase system and inhibition of inhibitory protein IkappaBalpha required for formation of AP-1 and NFkappaB, respectively
Chen et al., J Biol Chem 1998
:
Taken together, these data demonstrate that nucleotides, especially UTP, can potentiate the
LPS induced activation of NF-kappaB and
AP-1 and of iNOS induction via a CaMK -dependent pathway and suggest that the UTP dependent up-regulation of iNOS may constitute a novel element in the inflammatory process
Hall et al., J Biol Chem 1999
:
These data indicate that
LPS induction of TF gene expression in monocytic THP-1 cells is
regulated by both the transient phosphorylation of
Jun-family proteins and the nuclear translocation and transient binding of NFkappaB/Rel proteins
MacKichan et al., J Biol Chem 1999
:
We found that while both C2-ceramide and
LPS activated
c-Jun N-terminal kinase (JNK) , only LPS also activated extracellular signal regulated kinases ( ERKs )