◀ Back to BRAF
BRAF — RAF1
Pathways - manually collected, often from reviews:
-
NCI Pathway Database PDGFR-beta signaling pathway:
RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
→
RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, collaborate)
Garnett et al., Mol Cell 2005, Rushworth et al., Mol Cell Biol 2006, Ritt et al., Mol Cell Biol 2010
Evidence: physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
→
BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, collaborate)
Garnett et al., Mol Cell 2005, Rushworth et al., Mol Cell Biol 2006, Ritt et al., Mol Cell Biol 2010
Evidence: physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
→
BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, collaborate)
Garnett et al., Mol Cell 2005, Rushworth et al., Mol Cell Biol 2006, Ritt et al., Mol Cell Biol 2010
Evidence: physical interaction
-
NCI Pathway Database ErbB1 downstream signaling:
RAF1 (RAF1)
→
BRAF (BRAF)
(modification, collaborate)
Rushworth et al., Mol Cell Biol 2006, Yasuda et al., J Biol Chem 2009*
Evidence: assay, physical interaction
-
NCI Pathway Database ErbB1 downstream signaling:
RAF1 (RAF1)
→
RAF1/BRAF complex (RAF1-BRAF)
(modification, collaborate)
Rushworth et al., Mol Cell Biol 2006, Yasuda et al., J Biol Chem 2009*
Evidence: assay, physical interaction
-
NCI Pathway Database ErbB1 downstream signaling:
BRAF (BRAF)
→
RAF1/BRAF complex (RAF1-BRAF)
(modification, collaborate)
Rushworth et al., Mol Cell Biol 2006, Yasuda et al., J Biol Chem 2009*
Evidence: assay, physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
KSR/14-3-3 /MEK1-2 complex (KSR1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB-MAP2K1_MAP2K2)
→
RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF)
(modification, collaborate)
Ory et al., Curr Biol 2003, McKay et al., Proc Natl Acad Sci U S A 2009, Rajakulendran et al., Nature 2009, Ritt et al., Mol Cell Biol 2010, Reuter et al., J Biol Chem 1995
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
Erk1-2-active (MAPK3/MAPK1)
→
RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, activates)
Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
Evidence: assay, physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
→
RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, collaborate)
Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
Evidence: assay, physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
RAF1/14-3-3 complex (RAF1-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
→
BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, collaborate)
Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
Evidence: assay, physical interaction
-
NCI Pathway Database PDGFR-beta signaling pathway:
RAF1/BRAF/14-3-3/14-3-3 complex (RAF1-BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
→
BRAF/14-3-3 complex (BRAF-YWHAH_YWHAZ_YWHAQ_SFN_YWHAE_YWHAG_YWHAB)
(modification, collaborate)
Brummer et al., Oncogene 2003, Dougherty et al., Mol Cell 2005, McKay et al., Proc Natl Acad Sci U S A 2009, Ritt et al., Mol Cell Biol 2010
Evidence: assay, physical interaction
-
NCI Pathway Database ErbB1 downstream signaling:
Erk1-2-active (MAPK3/MAPK1)
→
RAF1/BRAF complex (RAF1-BRAF)
(modification, activates)
Rushworth et al., Mol Cell Biol 2006, Wu et al., J Biol Chem 1996*
Evidence: assay
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Bind Interaction:
RAF1
—
BRAF
Wan et al., Cell 2004*
-
IRef Bind_translation Interaction:
RAF1
—
BRAF
(coimmunoprecipitation)
Wan et al., Cell 2004*
-
IRef Biogrid Interaction:
RAF1
—
BRAF
(physical association, affinity chromatography technology)
Kilili et al., J Biol Chem 2010*
-
IRef Biogrid Interaction:
RAF1
—
BRAF
(physical association, affinity chromatography technology)
Chen et al., J Biol Chem 2008
-
IRef Biogrid Interaction:
RAF1
—
BRAF
(physical association, affinity chromatography technology)
Lito et al., Cancer Cell 2012*
-
IRef Biogrid Interaction:
RAF1
—
BRAF
(physical association, affinity chromatography technology)
Weber et al., Cancer Res 2001*
-
MIPS CORUM MAP2K1-BRAF-RAF1-YWHAE-KSR1 complex:
MAP2K1-BRAF-RAF1-YWHAE-KSR1 complex complex (BRAF-KSR1-MAP2K1-RAF1-YWHAE)
Gloeckner et al., Proteomics 2007
-
MIPS CORUM BRAF-RAF1-14-3-3 complex:
BRAF-RAF1-14-3-3 complex complex (BRAF-RAF1-SFN-YWHAB-YWHAE-YWHAG-YWHAH-YWHAQ-YWHAZ)
Chen et al., J Biol Chem 2008
-
MIPS CORUM RAF1-BRAF complex, RAS stimulated:
RAF1-BRAF complex, RAS stimulated complex (BRAF-RAF1)
Garnett et al., Mol Cell 2005
-
IRef Corum Interaction:
RAF1
—
BRAF
(association, anti tag coimmunoprecipitation)
Garnett et al., Mol Cell 2005
-
IRef Corum Interaction:
Complex of BRAF-RAF1-KSR1-YWHAE-MAP2K1
(association, tandem affinity purification)
Gloeckner et al., Proteomics 2007
-
IRef Corum Interaction:
Complex of RAF1-BRAF-YWHAQ-YWHAB-SFN-YWHAG-YWHAE-YWHAZ-YWHAH
(association, anti tag coimmunoprecipitation)
Chen et al., J Biol Chem 2008
-
IRef Dip Interaction:
RAF1
—
BRAF
(direct interaction, anti bait coimmunoprecipitation)
Hatzivassiliou et al., Nature 2010*
-
IRef Dip Interaction:
RAF1
—
BRAF
(direct interaction, anti bait coimmunoprecipitation)
Hatzivassiliou et al., Nature 2013*
-
IRef Dip Interaction:
RAF1
—
BRAF
(direct interaction, anti tag coimmunoprecipitation)
Hatzivassiliou et al., Nature 2010*
-
IRef Dip Interaction:
Complex of BRAF-ARAF-RAF1
(anti bait coimmunoprecipitation)
Hatzivassiliou et al., Nature 2010*
-
IRef Dip Interaction:
Complex of BRAF-MAP2K1-RAF1
(anti bait coimmunoprecipitation)
Hatzivassiliou et al., Nature 2013*
-
IRef Hprd Interaction:
RAF1
—
BRAF
(in vivo)
Weber et al., Cancer Res 2001*, Karbowniczek et al., J Biol Chem 2006*
-
IRef Intact Interaction:
RAF1
—
BRAF
(physical association, anti bait coimmunoprecipitation)
Heidorn et al., Cell 2010*
-
IRef Intact Interaction:
RAF1
—
BRAF
(physical association, anti tag coimmunoprecipitation)
Heidorn et al., Cell 2010*
-
IRef Intact Interaction:
RAF1
—
BRAF
(physical association, anti bait coimmunoprecipitation)
Packer et al., Cancer Cell 2011*
-
IRef Intact Interaction:
RAF1
—
BRAF
(physical association, anti tag coimmunoprecipitation)
Packer et al., Cancer Cell 2011*
-
IRef Intact Interaction:
RAF1
—
BRAF
(physical association, anti tag coimmunoprecipitation)
Röring et al., EMBO J 2012*
-
IRef Intact Interaction:
RAF1
—
BRAF
(physical association, anti bait coimmunoprecipitation)
Holderfield et al., Cancer Cell 2013*
-
IRef Intact Interaction:
Complex of HRAS-RAF1-BRAF
(physical association, anti bait coimmunoprecipitation)
Packer et al., Cancer Cell 2011*
-
IRef Intact Interaction:
Complex of 143 proteins
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of 78 proteins
(association, tandem affinity purification)
Gloeckner et al., Proteomics 2007
-
IRef Intact Interaction:
Complex of 26 proteins
(association, anti tag coimmunoprecipitation)
-
IRef Intact Interaction:
Complex of 11 proteins
(association, tandem affinity purification)
Gloeckner et al., Proteomics 2007
-
IRef Ophid Interaction:
RAF1
—
BRAF
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
Mizutani et al., FEBS Lett 2001
:
Involvement of
B-Raf in Ras induced
Raf-1 activation
Banihashemi et al., Mol Endocrinol 2002
:
TRH induced
c-Raf but not B-Raf activation, and the D2S receptor
inhibited both TRH induced c-Raf and basal
B-Raf kinase activity ... TRH induced c-Raf but not
B-Raf activation, and the D2S receptor
inhibited both TRH induced
c-Raf and basal B-Raf kinase activity
Röring et al., EMBO J 2012
(MAP Kinase Signaling System) :
In contrast, B-Raf ( wt ) and Raf-1 ( wt ) mediated signalling triggered by oncogenic Ras as well as the paradoxical
activation of
Raf-1 by kinase inactivated
B-Raf require an intact DIF. Surprisingly, the B-Raf DIF is not required for dimerisation between Raf-1 and B-Raf, which was inactivated by the D594A mutation, sorafenib or PLX4720
Tombes et al., Biochem J 1998
:
In contrast to hepatocytes, NGF treatment of PC12 pheochromocytoma cells caused large sustained activations of
B-Raf and p42 ( MAPkinase ), and a lower phasic
activation of
Raf-1