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IGF1 — RUNX2
Pathways - manually collected, often from reviews:
Text-mined interactions from Literome
Pei et al., Acta Pharmacol Sin 2003
(MAP Kinase Signaling System) :
IGF-I , GM-CSF, and EGF could
increase the promoter activity and the mRNA expression of murine
Cbfalpha1 gene in MC3T3-E1 and C2C12 cells
Qiao et al., J Biol Chem 2004
:
Insulin-like growth factor-1 regulates endogenous
RUNX2 activity in endothelial cells through a phosphatidylinositol 3-kinase/ERK dependent and Akt independent signaling pathway ... Here we show that
IGF-1 and its receptor
regulate post-translational changes in
RUNX2 to activate DNA binding in proliferating EC ... The phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002, reduced both basal and
IGF-1 stimulated
RUNX2 DNA binding activity in the absence of changes in RUNX2 protein as did the overexpression of the phosphatidylinositol 3-phosphate phosphatase, confirming that PI3K signaling mediates RUNX2 activation ... Treatment with U0126, which inhibits ERK1/2 activation, reduced
IGF-1 stimulated
RUNX2 DNA binding without affecting RUNX2 protein levels ... Surprisingly, these
IGF-1 mediated effects on
RUNX2 were not regulated by Akt phosphorylation, a common downstream target of PI3K, as determined by pharmacological or genetic inhibition ... However, an inhibitor of the p21 activated protein kinase-1, glutathione S-transferase-Pak1- ( 83-149 ), inhibited both basal and IGF-1 stimulated RUNX2 DNA binding, suggesting that Pak1 mediates
IGF-1 signaling to
increase RUNX2 activity ... These results indicate that the angiogenic growth factor,
IGF-1 , can
regulate RUNX2 DNA binding through sequential activation of the PI3K/Pak1 and ERK1/2 signaling cascade
Ochiai et al., J Biol Chem 2012
(Bone Diseases) :
Inhibition of
IGF-1 signaling using small interfering RNA ( siRNA ) against insulin receptor substrate-1 (IRS-1)
suppressed mRNA expression of
RUNX2 , ALP, BSP, and IGF-1 even with single TGF-ß1 administration