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RELA — WNK1

Text-mined interactions from Literome

Hamanoue et al., Mol Cell Neurosci 1999 : Overexpression of either the p65 or the p50 NF-kappaB subunits resulted in NF-kappaB activation and promoted in vitro survival as effectively as NGF
Mandrekar et al., Int Immunol 1999 : We show here for the first time that alcohol inhibits lipopolysaccharide (LPS) induced NFkappaB activation in human monocytes by decreasing DNA binding of the p65/p50 heterodimer as seen in electrophoretic mobility shift and supershift assays
Vasiliou et al., Biochem Biophys Res Commun 2000 (Fibrosis...) : The differential NF-kappaB binding observed in these two cell lines appears to depend on the phosphorylation of the p65 subunit rather than the expression levels of either p65 or p50
Yan et al., J Biol Chem 2001 (Neoplasm Invasiveness) : KiSS-1 represses 92-kDa type IV collagenase expression by down regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha -induced block of p65/p50 nuclear translocation
Helenius et al., Antioxid Redox Signal 2001 : The up-regulation of constitutive nuclear NF-kappa B binding activity and increased levels of nuclear p52 and p65 proteins might affect the expression of some NF-kappa B target genes in the aging liver
Lee et al., Biochem Biophys Res Commun 2002 : Electromobility shift assays showed that the expression of p65 induced the binding of the p65/p65 homodimer to the NF-kappaB site at -1180
Johannessen et al., J Gen Virol 2003 : Mutation of the TATA box had no effect on TNFalpha- or RelA/p65 mediated induction of NFkappaB-responsive promoters, indicating a specific st-ag effect on hTAF ( II ) 130/135
Gan et al., Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2003 (Colitis, Ulcerative) : The results showed that NF-kappa B p65 antisense oligonucleotides resulted in down-regulation of NF-kappa B p65 expression, blocked the expression of IL-1 beta mRNA and IL-8 mRNA, and strikingly reduced the production of IL-1 beta and IL-8, and these effects were greater than those of dexamethasone in cultured LPMC from patients with UC ( P < 0.05 )
Kim et al., Br J Pharmacol 2003 : In conclusion, Rg3 relaxes vessels as a consequence of NO production, to which iNOS induction contributes, and iNOS induction by Rg3 accompanied NF-kappaB activation, which involves phosphorylation and degradation of I-kappaBalpha and nuclear translocation of p65
Lee et al., Biochem Pharmacol 2003 : Further analysis revealed that manassantins A and B dose-dependently suppressed not only the induced NF-kappaB activation by overexpression of RelA/p65 , but also transactivation activity of RelA/p65 ... Further analysis revealed that manassantins A and B dose-dependently suppressed not only the induced NF-kappaB activation by overexpression of RelA/p65 , but also transactivation activity of RelA/p65
Takada et al., J Biol Chem 2004 : PTD-p65-P1 suppressed NF-kappaB activation induced by lipopolysaccharide, interleukin-1, okadaic acid, phorbol 12-myristate 13-acetate, H ( 2 ) O ( 2 ), and cigarette smoke condensate as well as that induced by TNF
O'Mahony et al., J Biol Chem 2004 (Cell Transformation, Viral) : Human T-cell lymphotropic virus type 1 tax induction of biologically Active NF-kappaB requires IkappaB kinase-1 mediated phosphorylation of RelA/p65
Kim et al., Biochem Biophys Res Commun 2004 (Colorectal Neoplasms) : Furthermore, CDX2 was found to co-immunoprecipitate with the p65 subunit of NF-kappaB and to inhibit p65 induced NF-kappaB minimal promoter activity in colon cancer cells
Monaco et al., Proc Natl Acad Sci U S A 2004 (Arteriosclerosis...) : We found that NF-kappa B is activated in these cells and that this activity involves p65 , p50, and c-Rel but not p52 or RelB
Hu et al., Carcinogenesis 2004 (Cell Transformation, Neoplastic) : These findings provide direct evidence that phosphorylation of p65 at S536 is required for TNF-alpha induced NF-kappaB activation in the JB6 transformation model
Park et al., Biochem Biophys Res Commun 2004 : A competition assay indicated that not only NF-kappaB-p65 but also -p50 is involved in 6-OHDA induced NF-kappaB activity
Jung et al., Int J Oncol 2004 (MAP Kinase Signaling System) : The cIAP1, cIAP2 and XIAP could enhance the NF-kappaB transcription and the chimeric NF-kappaB-p65 transactivation
Tas et al., Eur J Immunol 2005 : NF-kappaB inhibition by the NBD peptide resulted in blockade of IKK mediated IkappaBalpha phosphorylation and subsequent nuclear translocation and DNA binding of NF-kappaB p65 in DC
Kim et al., Cancer Res 2005 (Carcinoma, Hepatocellular...) : Cleavage-resistant p65D97A further increased NF-kappaB activity in TRAIL treated cells, whereas the COOH-terminal p65 fragment acted as a dominant negative inhibitor
Zheng et al., Virchows Arch 2006 (Lupus Nephritis...) : In contrast to non-proliferative glomerulopathy and normal controls, NF-kappaB activation ( both p65 and p50 ) was enhanced in glomerular endothelial, mesangial cells or infiltrating cells in class IV LN, along with upregulation of TNF-alpha, IL-1beta, IL-6 and ICAM-1 expression
Gomez et al., J Immunol 2005 (Arthritis, Rheumatoid...) : In contrast, depletion of endogenous PGE2 by ibuprofen ( 100 microM ) and celecoxib ( 5 microM ) enhanced p65 , but inhibited p50 nuclear translocation as well as binding to NF-kappaB DNA binding sites
Murakami et al., J Nutr 2005 (Neoplasms) : Pretreatment of RAW264.7 cells with LPS led to the activation of mitogen activated protein kinase ( MAPK ) s [ p38, extracellular signal regulated kinase ( ERK ) 1/2, c-Jun NH2-terminal kinase (JNK)1/2 ] and Akt, together with degradation of the inhibitor of nuclear factor-kappaB ( IkappaB)-alpha protein and nuclear translocation of nuclear factor (NF)-kappaB p65 , and the resultant activation of activator protein (AP)-1, NF-kappaB , and cAMP-responsive element binding protein ( CREB ) transcription factors
Grassl et al., Int J Med Microbiol 2005 : By means of electrophoretic mobility shift assays we demonstrate that sesquiterpene lactones inhibit Yersinia triggered activation of NF-kappaB by inhibiting Rel p65 , but not Rel p50
Sanda et al., Leukemia 2006 (Leukemia-Lymphoma, Adult T-Cell) : In this study, we demonstrate that NF-kappaB is constitutively activated in various HTLV-1 infected T-cell lines and ATL derived cell lines irrespectively of Tax expression as evidenced by the phosphorylation of IkappaBalpha and p65 subunit of NF-kappaB, activation of NF-kappaB DNA binding, and upregulation of various target genes including bcl-xL, bcl-2, XIAP, c-IAP1, survivin, cyclinD1, ICAM-1 and VCAM-1
Takada et al., J Immunol 2006 : AKBA did not directly affect the binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase (IKK), IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation
Wang et al., Br J Pharmacol 2006 (Hyperplasia) : 7. Thus, NF-kappaB activation during TMCH involves both IkappaB alpha degradation and p65-Ser536 phosphorylation
Ralstin et al., Mol Cancer Res 2006 (Carcinoma, Hepatocellular) : The response of PLC cells to NS398 was augmented by p65 small interfering RNA to inhibit NF-kappaB p65 protein expression
Moon et al., Free Radic Biol Med 2006 : Results were supported by NF-kappaB activation as judged by reduced IkappaBalpha degradation and increased nuclear translocation of p65/RelA with LPS stimulation, serum deprivation, and PrxII deletion
Mishra et al., Int J Cancer 2006 (Carcinoma, Squamous Cell...) : The NF-kappaB DNA binding is primarily due to homodimerization of p50 but infection of high risk HPV promotes participation of p65 in NF-kappaB complex formation, leading to heterodimerization of p50/p65
Nair et al., J Immunol 2006 (Neoplasm Invasiveness) : Deguelin also suppressed the NF-kappaB reporter activity induced by TNFR1, TNFR associated death domain, TNFR associated factor 2, and IkappaBalpha kinase, but not that induced by p65
Shiah et al., Mol Cancer Ther 2006 (Pancreatic Neoplasms) : The inhibition of NF-kappaB activity is dependent on the down-regulation of nuclear phosphorylated p65 , a component of the active form of the NF-kappaB complex
Sen et al., Biochim Biophys Acta 2007 : NFkappaB p65 and IkappaB kinase (IKK) became phosphorylated and NFkappaB transcriptional activity became augmented during repetitive stress
Holmes et al., J Biol Chem 2007 : Although NFkappaB nuclear translocation is not altered, FoxP3 enhances NFkappaB-p65 binding to HIV-1 LTR
Geisler et al., Gastroenterology 2007 (Drug-Induced Liver Injury) : RelA/p65 is essential for TNF induced NF-kappaB activation in adult hepatocytes
Laga et al., Biochem Pharmacol 2007 : In this study, we show that methylglyoxal inhibits TNF induced NF-kappaB activation and NF-kappaB dependent reporter gene expression by inhibiting the DNA binding capacity of NF-kappaB p65
Nakamura et al., Rheumatol Int 2007 (Sjogren's Syndrome) : Chemical inhibition of PI3K-Akt by LY294002/wortmannin did not affect EGF mediated NF-kappaB p65 nuclear translocation ; and NF-kappaB inhibition by Bay 11-7082 did not suppress Akt phosphorylation
Wan et al., Cell 2007 : RPS3 knockdown impaired NF-kappaB mediated transcription of selected p65 target genes but not nuclear shuttling or global protein translation
Young et al., J Invest Dermatol 2008 (Psoriasis...) : Employing a series of in vitro and in cellulo approaches, we have demonstrated that in primary human keratinocytes ( i ) TNF-alpha rapidly induces ROS generation, IkappaB degradation, NF-kappaB p65 nuclear translocation, and ultimately production of inflammatory cytokines ; ( ii ) TNF-alpha induced cytokine production is mediated both by the mammalian target of rapamycin signaling pathway via NF-kappaB activation and by ROS ; ( iii ) TNF-alpha dependent NF-kappaB activation ( that is, IkappaB degradation and NF-kappaB p65 nuclear translocation ) is not mediated by ROS ; and ( iv ) a cell penetrating derivative of the antioxidant enzyme, catalase, as well as taurine and N-acetyl-cysteine attenuate the TNF-alpha induced production of cytokines
Yun et al., Mol Carcinog 2009 (Colonic Neoplasms) : The immunoblot, ELISA and EMSA analysis demonstrated that the treatment of HCT116 cells with delphinidin resulted in the inhibition of ( i ) IKKalpha, ( ii ) phosphorylation and degradation of IkappaBalpha, ( iii ) phosphorylation of NF-kappaB/p65 at Ser ( 536 ), ( iv ) nuclear translocation of NF-kappaB/p65, ( v ) NF-kappaB/p65 DNA binding activity, and ( vi ) transcriptional activation of NF-kappaB
Kasperczyk et al., FASEB J 2009 (Neoplasms) : Further, NF-kappaB activation by tumor necrosis factor alpha (TNF-alpha) or p65 overexpression stimulates Shh promoter activity and p65 binds to Shh promoter in vivo
Takeshima et al., BMC microbiology 2009 (Helicobacter Infections) : PI3K inhibitor suppressed H. pylori induced p65 phosphorylation and NF-kappaB transactivation , as well as interleukin-8 expression
Gu et al., Nature 2009 (Cystic Fibrosis...) : Neutrophils, but not macrophages, from Ifrd1-deficient mice showed blunted effector function, associated with decreased NF-kappaB p65 transactivation
Voegeli et al., Inflamm Res 2009 : The proinflammatory effects of Ang II on NF-kappaB in vascular smooth muscle cells may be through a non-canonical pathway and be dependent on p65 phosphorylation
Fazal et al., J Biol Chem 2009 : The effect of cofilin-1 depletion on NF-kappaB activity and ICAM-1 expression occurred downstream of IkappaBalpha degradation and was a result of impaired RelA/p65 nuclear translocation and consequently, RelA/p65 binding to DNA
Li et al., J Ethnopharmacol 2009 : The inhibitory effect of TMP on NF-kappaB ( p65 ) activity was mediated by blocking the consequent translocation of p65 into the nucleus
Bai et al., Int J Cancer 2009 (Cell Transformation, Neoplastic) : Our data suggest that the stimulation of NFkappaB by Akt is dependent on the phosphorylation of p65 at S534, mediated by IKK ( IkappaB kinase) alpha and beta
Cristofanon et al., Ann N Y Acad Sci 2009 : ( 1 ) Here we show that melatonin induces a strong canonical activation of NF-kappaB , inducing IkappaBalpha degradation and the consequential nuclear translocation of p50/p65 subunits
Cha et al., Immunol Invest 2009 (Inflammation) : Western blot analysis showed that the essential oil inhibited the phosphorylation of IkappaB-alpha, nuclear translocation of p65 , and subsequent activation of NF-kappaB
Yang et al., J Cell Physiol 2010 (Arthritis, Rheumatoid) : IL-1beta induced ICAM-1 expression, extracellular signal regulated kinase ( ERK ) and c-Jun-N-terminal kinase (JNK) phosphorylation, AP-1 activation, and nuclear factor-kappaB (NF-kappaB) p65 translocation were attenuated by the inhibitors of MEK1/2 ( U0126 ), JNK ( SP600125 ), AP-1 ( tanshinone IIA ), and NF-kappaB ( helenalin ) or transfection with respective short hairpin RNA plasmids
Han et al., Biol Pharm Bull 2010 : Moreover, compounds 1 and 2 attenuated NF-kappaB activation by inhibition of IkappaBalpha proteolysis and p65 nuclear translocation, as well as phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2, c-Jun N-terminal kinase (JNK), and p38 MAP kinases
Wang et al., J Neurosci 2010 : We found that downregulating the expression of RelA/p65 subunit of NF-kappaB or inhibiting NF-kappaB activity by overexpression of mutated form of IkappaB ( inhibitor kappaB ), which is resistant to proteolytic degradation and thus constitutively keeps NF-kappaB inactive in the cytoplasma, impeded the formation of AChR clusters in cultured C2C12 muscle cells stimulated by Agrin
Posadas et al., PloS one 2012 (Neuroblastoma) : AAP also induced NFkB p65 activation by phosphorylation and its translocation to the nucleus, where NFkB p65 increased IL-1ß production
Thanos et al., Mol Cell Biol 1995 (Virus Diseases) : We conclude that the p50/p65 heterodimer is responsible for the NF-kappa B-dependent activation of the IFN-beta gene promoter in response to virus infection
Scheinman et al., Mol Cell Biol 1995 : In transfection experiments, DEX treatment in the presence of cotransfected glucocorticoid receptor ( GR ) inhibits NF-kappa B p65 mediated gene expression and p65 inhibits GR activation of a glucocorticoid response element
Schafer et al., Virology 1996 : Thus it appears that p65 plays a limited role in the NF-kappa B-dependent activation of the HIV-1 LTR following HSV-1 infection and that the stimulation is mediated by the p50/p65 and p65/c-rel heterodimers
Kurokouchi et al., J Bone Miner Res 1998 (Bone Neoplasms...) : These results suggest that the rapid activation of NF-kappaB by TNF-alpha is mainly due to the nuclear translocation of NF-kappaB pre existing in cytosol, and that the subsequent increase in the expression of p50 and p65 may result in the persistent activation of NF-kappaB during TNF-alpha stimulation