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Multiz Alignments of 7 Yeasts
 

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Alignment block 1 of 31 in window, 765966 - 767003, 1038 bps 
B D  S. cerevisiae  agaagataacgaggaaga---------------------tgaagacgaggttgaatatgacgaccatggt
            sacPar  agaagatgacgaggaagag---------------ggagatgaagacgaggttgaatatgatgaccatggt
            sacMik  tgaagatggcgatgaaga---------------------tgaagacgaggttgaatatgatgaccatggt
            sacKud  agaagacgaaggcgggga---------------------tgaagaagagattgagtacgacgataatggc
            sacBay  tgaagatgaagacgaagaggaggatgaaaacgaagaggaagaagaggaggatgagtatgatgacaacgga
            sacCas  cgaggatgcagatgaagaagttgatgaaacgtatgg---tgaggatgcacctgaatttgatgaccatggg
            sacKlu  agaagacggtgatgataataa------------------cgaagacgagcaagaatatgatgaccacggt

     S. cerevisiae  aataaagtactgaaaaagtttgaaaacctgccagatttaataagctttaaggggtacttgcctgaattaa
            sacPar  aataaagtactgaaaaagtttgaaaacctgcctgatctcataagtttcaaggtatacctgcccgaactga
            sacMik  aatagagtgctgaagaagttggaaaacctgccagatttaattagcttcaaaggatacttgcctgaattaa
            sacKud  aatagaatactgaagaagtttgaaaacctgccagatatggttagcttcaagggatatttgcctgaattaa
            sacBay  aataagatactgaagaagttccaaaacctgcccgatacggttagttttaagggatacctacccgaattaa
            sacCas  aatagaatactgaataaattagaaaacttaccagataagataagtttccaacaatatattcctgatttac
            sacKlu  aatccaattgtctcgacattggagaatttgcccgacaaaatccattttgatgatttcattcctactctat

     S. cerevisiae  ccgtacaattagaatcgcaaatcgaaaccgatatgttattgggtatgcaaatgtgttttgcattgatttc
            sacPar  ccgtacaattggaatcgcaaattgaaaccgatatgctattaggtatgcaaatgtgttttgcgttaatttc
            sacMik  ctgtacagttagaaacccaaatcgaaaccgacatgttactgggtatgcaaatgtgttttgcattaatttc
            sacKud  ccgtacagttggaaacgcaaatcgaaaccgacatgttactgggcatgcagatgtgtttcgcgttgatttc
            sacBay  ccgtaaagttggaaacgcaaatcgaaacggacatgttactgggcatgcaaatgtgttttgcgctgatttc
            sacCas  aggtaaaattagaagaacaaattgaagatgatatgttaattggaatgcaaatgtgttttgctctgatttc
            sacKlu  tatcaaaagttgatggtcaggttgaagacgatatgcttttaggtatgcaaatgtgctttgcgataatttc

     S. cerevisiae  atccataaaagaaagatgtgagcaatggtatagtgaacaattgaataagctcgagaagcagtacgagctg
            sacPar  atctataaaagaaagatgcgagcagtggtatagtgagcaattgagcaagctcgagaaacagtgcgaaatg
            sacMik  atcaataaaagagagatgtgagcagtggtacagcgagcaattgagcaagctcgaagaacagcacgacctg
            sacKud  atcaatcaaggagaaatgtgaacagtggtatagcgagcaattgaacaagctcgagaaacaacacgatttg
            sacBay  gtcaataaaggagaactgtgaacagtggtacagcgaggaattgggcaaactggaaaaacaacacgattgg
            sacCas  ctccataaaagaacattgtgaaacgtggttttctgaacaattacatgaactagacagacaacatgaattg
            sacKlu  gaatattaaggaaacttcagaggcctggttccaggatacgttgacagaacttgaaaaagaacacgaacgt

     S. cerevisiae  gaagcacaagaacgtgaaaagaaggaacaggccaagttccatggtactaaagtcacaagagaatcgtact
            sacPar  gaggcacaagaacgtgaaaagaaggaacaggccaagttccacggtacaaaagttacaagagaatcgtatc
            sacMik  gaagcacaagaacgtgagaagaaggaacaagcgaagttccacggtacaaaagtgacaagagaatcatatt
            sacKud  gaggcacaagagcgagagaagaaggaacaggccaagttccacggtacaaaagtcacaagagaatcgtatt
            sacBay  gagactcaagagcgtgaaaagaaggaacaagccaagttccacggtacgaaggtaacaagagaaacgtatt
            sacCas  gagattcaaaaacgtgagaaggaagaacaagccaaatttaatggtaccaaagttactaaagattcattca
            sacKlu  aagcttctagatcgtgaaagagaagagcaaaagaaatttcggggaactaaagttacagccgaatcgtatt

     S. cerevisiae  tggaatggaggtctaaatttcgccaagaactgaaactggatgaaagagaccaggtgagaaggatgaaggc
            sacPar  tggaatggaggtccaagttccgtcaagaactgaaactggatgaaagagatcaggtgagaaggttgaaggc
            sacMik  tagaatggaggtctaagttccgcaaggaattgaaactggatgaaagagatcaggtgagaaggatgaaggc
            sacKud  tggaatggaggtctgagttccgtaaagaactgaaactggacgaaagagaccaagtgagaagaatgaaggc
            sacBay  tggaatggaggtcttccttccgcaaagagctaaaactggacgaaagagaccaagtaagaagaatgaaggc
            sacCas  ttgaatggagattaaaattcaggaaggaattgcaattagatactagggatgagatgagaaggttgcaagc
            sacKlu  tggcatggagaaccaaattcagaaaggatatgggactggatgaaagagacaatcaaaggaggttggatgc

     S. cerevisiae  tcatcatgggaagctaaccggtaagcaaatgtttgaacaaggcgtggtgggcacaggcgacg--------
            sacPar  ccaccatgggaagctaactggtaagcaaatgtttgaacaaggtgtggtgggcacgggcgacg--------
            sacMik  tcatcacgggaagctaaccggtaagcaaatgtttgaacaaggagtggtaggcacgaacgacg--------
            sacKud  ccatcacgggaaactgaccgggaagcaaatgttcgaacaaggtgtggtgggcacgggcgacg--------
            sacBay  gcaccacgggaagctgacggggaaacaaatgttcgaacaaggtgtggtgggcacaggcgacg--------
            sacCas  tcatcatggtagattaactggtaaacaaatgttcgaacaaggtgtagacggtaccattgacgatttagat
            sacKlu  acataacggaaggttgtccggtaaacaaatcttcgagcaaggtttggacggtggggacgacg--------

     S. cerevisiae  ----agtatatggaagaggatgacgcgagtgtagatgac---gtagccaagggacttgccaagaccgaaa
            sacPar  ----agtatatggaagaggatgacgcgagcgtggatgac---gtagccaagggacttgccaagactgaaa
            sacMik  ----aatatatggaagaagatggcgcgacagtggatgac---gtagccaaggaacttgccaagactgaaa
            sacKud  ----agtacatggaagaggatggcgcgagcgtggatgac---gtagccaagggacttgccaagactgaaa
            sacBay  ----atttcatagaggaggatggcacgagcgtggacgat---gtgtccaaggccctcgccggtactgaac
            sacCas  gagaattttgaagatgagagtagcattagcattaatgatcaagtagccaaggttactgacgat-ttgaaa
            sacKlu  ----ccgaggttgaagaa------------------gac---ctggccgaag------------------

     S. cerevisiae  tagc--------aaatcaataaa-tacttgtgtacttaagtatttaagtattttcttctgcatgg-at-a
            sacPar  tagc--------aaattaataaaatatttatatacttgagaatttaagtgttttcttctgcatgg-at-a
            sacMik  tagc--------taaacaataaa---------caattaagtatctaaatactttcttctgcatggaat-a
            sacKud  tagt--------aaatcaatgaa---------tatttaagtatttaagtattttcttctgcatgg-at-a
            sacBay  tagt--------aaatcaatgaa---------tacttaagtacttacgtatttt-ttttgcatag-ataa
            sacCas  gaactatagacaaaagtagcaaa--------------aattatctaagaa-----------gggg-tt-a
            sacKlu  ------------gtatcaagaag------------------ctataattacctcc---------g-at-a

     S. cerevisiae  atgacatatacaaatgtatctatataatatttttttgatattcaataaaaagaaaactgacatttgaaga
            sacPar  atgacatatc------tatatatacaattctttcttattgttcaattaaaagaaaagtgatatttgaggg
            sacMik  atgacataag------------tataat--ttttctgttgttaaataaaaaaaaa---tagattcgaaga
            sacKud  atgacataca------------tacatctttctcattttgttttcgtaaaaacta-gtagtaccaaagga
            sacBay  atgacatatatatatatatatatagatatatatttggttgtttcaaataatagta--------ttgaaga
            sacCas  ataaaatgag------------gatgac----------tatttaatagaaataaa--gtacatgtgtatg
            sacKlu  atgcaacaca------------actgtgtattttc-----------------------------------

     S. cerevisiae  aggaaatagacaagtttttagtaagatattatt-----agtgtat-----atatggatacatatatctga
            sacPar  gagaa-taaacaatttctta-taagatattatt-----agtgtat-----atatggatacatatatctga
            sacMik  aggaa-taaacaa--ttttaataaggtattatt-----agtgtat-----gtatgtgtacatatatttga
            sacKud  aggaa-tgaacaa--ttccaataaaatattatta---aaatgtgt-----atatgtgtacatatatctaa
            sacBay  aagga-aggacag---tttaataagacaatattaaagaaatgtatataa-atatgtatacatatatcgga
            sacCas  gtgta-ttaacaaactaggaagaaaaaagtatt-----agt-tatttaacaaatcggcttagacatcaaa
            sacKlu  ------tctacatattttt---------ttttt-----accctgt-----aca---------atacttaa

     S. cerevisiae  -gaatgaaaatttgt-tgactgtattgaa---tgtattgg-gagatgaa---ggggtatttggtg-----
            sacPar  -gaatgaaaagttgtgctactgtattgaa---tgtattgaagagatgga---tgtgtggttggtg-----
            sacMik  -gaatgaaaagtggtggtgctgtattgaa---gttattgtggacgtgga---ggtgtgcttgttg-----
            sacKud  -----------ttgtgctgccgtacagaattttttattgcggggggggaaggggcgaaattggaggttca
            sacBay  -gaatgaataatcgtgctgctgtattgaa---aatttcgg------------ggtgtcgtt---------
            sacCas  taaatgcaaatccttgt---------------tgtttcgg------------gtagaagtt---------
            sacKlu  ----------------------------c---tatctcac------------------gtt---------

     S. cerevisiae  ----attagaccaacgacttggagtaagagaataagtcggaaaatgcatcatttcccttc------gact
            sacPar  ---cactagaccaacgactttgaataagaaaataggtccgaaaatgcgtcattccccttt------gact
            sacMik  ---cactagaccaacgacttggaataagagaataagtcagagaaggcatcgttccccttt------gact
            sacKud  aaacgctaaaccaatgacttggagtaggagaataagttagaaaaggcgtcgttgcccttt------gact
            sacBay  ------tagaccaatgacttggagtaagagaaaaggtcggaaaaggcatcgttgcctttg------gtct
            sacCas  ------tatatcaatgatttagaattagtgaaaagatcggcaaatggatcttttttcgttgcagaagagg
            sacKlu  ------tatacgtgtgacttcgaatatgaaaacaaatcggcaaaagcatcctttttgtct------ga--

     S. cerevisiae  tgtcctttgtag-----cctgagga--cgggctttttgctgtttt----ttctccgcaaagggcaatgat
            sacPar  tgtcctttgtag-----cttgggga--cgggctttttgttggttt----ttctccgcaaagggtaatggt
            sacMik  tgtctttggtag-----cttgagga--cgagccttttgttgcttc----ttctccgcaaaaggtagcgat
            sacKud  tatcctttgtgg-----cctgaggg--cgagatttttgctgcttc----gtttcggaaaaggataatgag
            sacBay  tgtcctttgtag-----cttgagga--cgggcttttgattgcgta----ttctcggcaagaggcagtgac
            sacCas  tattcgtggtggtattatttgtggt--cgtagcatcagaagagttaccatttcctccaaagtctaataaa
            sacKlu  -------ggtag--------aaggatttgaatcattcgctgctgg----tgtggtgggaactgtg--gac

     S. cerevisiae  gtttgcgcagcagcggtggtatctgtgtctgactttgggccatcccagtcaagcaaatcatctattt---
            sacPar  gcttctgcggcagtagtggtacctgcgcttgattttggggcgtcccagtcaagcaaatcatctattt---
            sacMik  gcttctgtggcagcggtggtatccgcgcttgattttggaccatcccagtcaagcaaatcatctattt---
            sacKud  gcatctgtagcggcggtggaggtggaacttggttttgggccatcccagtcaagcaagtcatctattt---
            sacBay  gcttcggtagcggcggtggtgtctgcactcgattttggaccatcccagtctagcaggtcatcgattc---
            sacCas  tcatccaagttagcgcttgta-------ttggttgtgga-------agccgaagtagtggtagattgtgg
            sacKlu  gtattattgctggtgttggca---------------agtccatcccagtccaagagatcgttcatattag

     S. cerevisiae  ----tactat--tggaag---ttttaggtgc---tgttgtgtcgggactcgtttcgctttggaagtcgcc
            sacPar  ----gactgt--tggaagaagttttgggtgc---tgtggtgtcaggacttgtttcgctttggaaatcacc
            sacMik  ----ggctat--tagatg---gttttggcgc---cgttgtgttcggactcgcttcgctttgaaaatctcc
            sacKud  ----ggccat--ctgatg---ttttgggcac---tgcggtgtcgggacttgtctcgctttggaaatcccc
            sacBay  ----gtccgc--cggttg---ttttaggggc---tgttgtgtcggggcttgcttcgctttggaaatcgcc
            sacCas  --tagggcat--tattag---ttttgattgc---tgttggtgcagggcttgcatcactctggaaatctcc
            sacKlu  aagaggctatagcaggag---gagcagatgcggttgttggttgaggactactttcactttgaaagtcacc

     S. cerevisiae  aaactcatcatcatcatcatcacta
            sacPar  aaactcatcatcatcatcatcatca
            sacMik  aaactcgtcatcatcatcatcatca
            sacKud  gaattcgtcctcatcatcattgctg
            sacBay  aaactcgtcgtcgtcgtcgtcgtcg
            sacCas  aaattcatcatcttcatcatcatcg
            sacKlu  gaattcatcttcatcttcgtcatca

Alignment block 2 of 31 in window, 767004 - 767108, 105 bps 
B D  S. cerevisiae  tt------------gctgttgatattattgtttttg---ttgtcattattaccattgtctattgttatat
            sacPar  tc---------------------atcattgttgctg---ttcacattattaccattgtctattgttatgt
            sacMik  tcatcatcatcgttatttttatcatcattgtttttgttattgtcattactaccatcatttatggttatgt
            sacKud  tc---------------------attaccgccactg---tttgtgttcttgtcgtcgctt---------t
            sacBay  tc---------------------atcgttgttgttg---ttgttgttgttattgttgtcattag---tgt
            sacKlu  ----------------------gctttttgagcttg---tag-----attcccact--------------

     S. cerevisiae  tattcg---tggtgctagtac--tatcgtcaagatcgattaagtc--agggatgcta
            sacPar  tgttcg---tagtgctagtac--tatcgtcaaaatcgattaaatc--agggatgtta
            sacMik  cgttcgtgatggtgtgagtat--tgtcgtcaaaatcgattaggtc--agggatacta
            sacKud  cgtgtgtgatggcggtgttag--catcatcgaaatcgattaggtc--agggatactg
            sacBay  tgttcg---tggtggtggaa-----tcatcgaagtcgattagatc--aggtatgttt
            sacKlu  ---------tggtgcggatgggacatcatcgaaatcgagtaagtctacagtaggctg

Alignment block 3 of 31 in window, 767109 - 767367, 259 bps 
B D  S. cerevisiae  tcttgttgttgctt----tcttttcctctgctgttctttatttttgatctgctctcttaatatctccctt
            sacPar  tcttgttcctgctt----tcttttcctctgttgttctttattcttgatctgctcccttaatatctctctc
            sacMik  tcttgctgttgttt----tctttttctttgttgttctttatttttgatctgctctcttaagatttctctt
            sacKud  tcttgctgttgttt----tctcttcctttgttgttctttgcttttgatctgttctcttaaaatctctctt
            sacBay  tcttcttgttgttt----tcttttcctttgctgttctttgtttttgatctgctctctcaggatttctctt
            sacCas  ttttgcaattgtt-------tcttcctttgttcttctgtgttcttaattctttccctgagtatttctctt
            sacKlu  ttctgttttggattgaggtgccttcctttgctgctcactggatctaatctgctctctaagcatctctctc

     S. cerevisiae  ctttgtcttctttgttcttccatatgagaacgcctgcgagtattcg------------------aatttg
            sacPar  ctttgtctcctttgttcttccatatgagaacgcctgcgaggattcg------------------aatttg
            sacMik  ctttgttttctttgttcttccatatgagaacgcctacgagtattgg------------------gaattg
            sacKud  ctttgtctcctctgttcctccatgtgggaaagcctgcgagtactgg------------------aatttg
            sacBay  ctttgtcttctttgttcgtccatgtgagaacgcctacgagtgcttg------------------aattcg
            sacCas  ctttgtcttctttgttctgtcaaattagataatctacgaggacgagttgatgatggaggtgctaaattag
            sacKlu  ctctgtcttctttgctgttctaaggcagaagatctgcgttggc--g------------------atgttc

     S. cerevisiae  cttctgtttccacaggaacattgaagttggtcacattattagcatccatttcactgtcaaagccatctgc
            sacPar  cttccgtttctacagggacattgaagttggtcacattatttgcgtccatttcactatcaaacccatctgc
            sacMik  cttctgtatctacgggcacattgaagttggtcacgttgttggcatccatttcatcatcaaccccatctgc
            sacKud  cctctgtttccatggggacattaaagttggtgacattgttcgtatccatgtcacccccaaccccatctgc
            sacBay  gttccgtttccacggggacgttgaagttggtgacgttgttggcgtctatttcatcgtcggaaccatcagc
            sacCas  cctctgtttcta-aatcaaattctaaatctttacctgatt--------cttgatcttcttcttcatcgtc
            sacKlu  ctgttgtttct---------------------------tttgtg-------------------caacttc

     S. cerevisiae  atctgcatcaaattcat------cgtcgtcgatgttatcgtaacttgcagacgtt---------------
            sacPar  atctgtgtcaaattcat------cgtcgtccacgttgtcgtaactggcagacgat---------------
            sacMik  atctgtatcaaattcat------catcatccacgttgtcgtagctagcagatgat---------------
            sacKud  atttgtaccaaaatcat------catcatcgtcgtcgtcgtagctggcggaagat---------------
            sacBay  gtccggcccaaaccctt------cgtcgtcgacactatcgtagttggcggaagag---------------
            sacCas  atc---accaaacccttgatctatatcgtttgcattatatgaggtacctgatggg---------------
            sacKlu  gtattcttcaaagtcat------cgaaatcgtcgtcgtaagagttggtggaagaggtaccgtatgaatcg

     S. cerevisiae  ---gaaatc-tggttg-----gcattataaa
            sacPar  ---gaaatc-tggttg-----gcattataaa
            sacMik  ---gagatt-tggttg-----gcgttataaa
            sacKud  ---gagatt-tggttg-----gcgttataaa
            sacBay  ---gagatt-tggttg-----gcgttgtaaa
            sacCas  ---gccatcattgttggagtagcattatata
            sacKlu  gtagtgatt-tggtta-----tcgttgta--

Alignment block 4 of 31 in window, 767368 - 767694, 327 bps 
B D  S. cerevisiae  tggaggattctcc-------ttg--------------ctgtcgaattttcaaggcattcttcttattctt
            sacPar  tggaggactcccc-------ttg--------------ctgtcgaatcttcaaggcgttcttcttattctt
            sacMik  tggaggattcgcc-------ttg--------------ctgtcggatcttcaaagcgtttttcttattttt
            sacBay  tggaggattcccc-------ttg--------------ctgtcggatcttcaaggcgttcttcttattctt
            sacCas  tagatgtctcagt-------ttg--------------ctgtcgaatctttgcagcattctttctgttctt
            sacKlu  -ggaggctccagcagcgacattggtagcagtgccggcttggtggatcttagcagtgttcttcttttgctt

     S. cerevisiae  ggctctttcctttttcaaaaattgtgagtcttccaagtattgcaagataagttctccctttttcctaata
            sacPar  ggctctttcctttttcagaaattgggggtcttccaagtattgcaagatcagttcgccctttttcctaata
            sacMik  ggctctttcctttttcaaaaactgagcgtcttccaagtactgtagaatcagttcaccctttttcctaata
            sacBay  ggctctttcctttttcaaaaactgagggtcttccaagtattgcaagatcaattcgccctttttcctgatt
            sacCas  ggatctttccttctttaagaaatgagggtcttccatatattgaagaatatcttcacccttcttcctgatc
            sacKlu  gactctttcacgtttcaggtagttgagatcttcgacgtattgaagaatctgttcacacttcttcttaata

     S. cerevisiae  cctctctcatgaatctccattttaccatcattggagaattttaccttgaattgggcaatctccctggtta
            sacPar  cctctctcatgaacctccattttgccatcatttgagaactttaccttgaattgggcaatctccctggtta
            sacMik  cctctctcgtgaatttccattttgccatcattggagaactttactttgaattgggcaatctctctggtta
            sacBay  cctctttcgtgaacctccatcttaccatcgtttgagaacttcaacttaaattgggcaatttctctagtca
            sacCas  ccacgttcatgaatttccattttcccatcatttgagaatttcactttaaaattggggatctccctcgtca
            sacKlu  ccgcgctcatggacttccatcttaccgtcgttactgaaatcgacgtggtattgcataatatcgcgggtca

     S. cerevisiae  aaatatgcttatgagtgagcaaacatgatctaatctgattcagttcgtcacctgtgtcgacgttcaataa
            sacPar  agatatgtttatgggtgagcaaacatgacctgatctgatttaattcgtcgcctgtatcgacattcaataa
            sacMik  aaatatgcttatgggtgagcaaacatgatctgatctggtttagttcatcgcctgtatcgacgttcagtaa
            sacBay  atatgtgtttgtgggtgagtaaacaagatctgatctgatttagttcgtcgcctgtgtcgacgttcagtaa
            sacCas  agatatgcttatgagtagtcaaacaactcaagacttgattcagttcatctccctcagagacattcaaaat
            sacKlu  agatgtgcttatgggtcaggatacagttcaccacctgatctaattcgttgcctacatcgacattcaacag

     S. cerevisiae  taggaattcaatgactactagacattttaagaccaccctccattcggacccataattaacgtagtcct
            sacPar  caagaattcaatgactactagacattttaagactactctccattcggacccgtagttaacgtagtcct
            sacMik  caagaattcaatcactgctagacattttaagaccactctccattcggacccgtaattaacgtaatctt
            sacBay  caagaactcgacgaccaccaggcacttcaagaccactctccattcggatccgtagttgacgtagtcct
            sacCas  caaaaaatcaatgacgactaaacatttcaaaacgactctccattcagccccataattaacatattctt
            sacKlu  catgtgctccaacaacaccaaacacttcagtactacacgccactcattcccgtaattgacatactgtt

Alignment block 5 of 31 in window, 767695 - 768024, 330 bps 
B D  S. cerevisiae  ttcttgccttttcgtataggttcttgggcctggtagcgatatggtcgactaatcttttcaacgtgtattc
            sacPar  ttcttgctttttcgtacaggttcttgggcctagtagcgatatggtcgaccaatcttttcaacgtatattc
            sacMik  ttcttgccttttcgtaaaaattttttggcctggtggcaatgtgatcgaccaatcttttcaaagtgtattc
            sacKud  tttctgccgtttcgtatagcttctt-ggtcttgaagcgatgtggtcgaccaatcttttcaaagtgtattc
            sacBay  ttcttgccttttcgtacaggttcttgggtctcgtggcgatgtggtcgaccagtctcttcagagtgtattc
            sacCas  ttctagccttctcataaaaattctttggtctagaggcaatatgatccactaatctcttcaaggtgtattc
            sacKlu  tgcgggccttttcatataaatttttgggccttgtagcgatgtgatcaacaagtcttttcaaaatatactc

     S. cerevisiae  tgtcatcaagtagagcggcacttggtatctgttccttaaaaccttggcgaggtgcttcggtgtggggccc
            sacPar  tgtcatcaagtatagcggtacttggtatctgtttcttaaaactttggccaggtgcttcggtgtggggccc
            sacMik  ggtcatcaagtatagtggcacttggtatctgtttcttaatactttggccagatgcttcggagtggggccc
            sacKud  ggtcatcaagtatagcggtacttggtatctgtttcttagaactttgcccagatgctttggcgtggggccg
            sacBay  ggtcatcaagtatagcggtacctggtacctgtttcttaagactttggccaaatgctttggcgtggggccc
            sacCas  caccatcaaatatagagggacttggtatctatttcttagcactttacttagatgttttggtgtggggccc
            sacKlu  ggtcattaggtaaaggggaacttggaatctgttgcgcatcactttgctcaagtgttttggcgtaggaccc

     S. cerevisiae  caagcatcggtatttgtagcacgacggatatctatctgataaggctcatattggactatcacgttttggg
            sacPar  caagcatcggtatttgtagcacgacggatatctatctgataaggttcgtattgaactatcacgttttggg
            sacMik  caagcatcggtgtttgtagcgcgacggatgtctatctgataaggttcgtattgaacaatcacgttctgag
            sacKud  caggcatcggtatttgtagcgcgacggatgtctacctgatagggctcatattggacgatcacgttctgag
            sacBay  caggcatcggtgtttgttgcacgacggatgtctacctggtagggttcgtattgtacaatcacgttttggg
            sacCas  caagcatctgtattggtggctctacggatgtccacctgatacggttcatattggacaatgacgttctgcg
            sacKlu  caagaatctgtgttagttgcacgacggatatccacttgataaggctcatattgcacgatcatgttctggg

     S. cerevisiae  caaaccttgcggcatttctaatatcatggatacctaaattttgaatcttttttgataatgagtccatttt
            sacPar  caaaccttgcggcatttctaatgtcatggatacctaagttttgaatcttcttcgataatgagtccatttc
            sacMik  caaacctggcggcatttcttatgtcatgcatgcctaagttttgaatcttcttcgataacgagtccattgg
            sacKud  caaatcttgcggcgttcctgatgtcgtgaatacccaggttttgaatcttcttcgataacgagtccatctc
            sacBay  cgaaccttgcggcgtttctaatgtcatgcatacccaggctctgaatcttcttcgataatgagtccatttt
            sacCas  caaaacgggctgcattacggatatcgtgaattcccaggttctggatcttcttggatagggagtccattgt
            sacKlu  cgaaacgcgccgcattacgaatatcatgaatccccaggttaccaatcttctttgatagcgagtccatttt

     S. cerevisiae  gtatggtgttggtatttatgcaattggaacgtttaattt-gtgtatatgaa
            sacPar  aggtggtattggtgtttgtgcttttgagatatttgattt-atgtagatgag
            sacMik  gggtgctgataatagtagtgatcctggg-tgcttggctt-atgtacttgcg
            sacKud  gt-tgttacttgtgttggagctatacggatgcttggtat-gagtgggcg--
            sacBay  ga-----ggtggtatttgtgtgggggaggtgctttgtgt-gtgtgtgtgtg
            sacCas  cggttgcgttggtggttaactaatgttggtccttgatccagtggatatgga
            sacKlu  tgtttttcctggttttc----------------------------------

Alignment block 6 of 31 in window, 768025 - 768033, 9 bps 
B D  S. cerevisiae  tttcccttc
            sacPar  tcctgcttc
            sacMik  tttcctttc
            sacBay  tgtgtgttg
            sacKlu  tttcccttc

Alignment block 7 of 31 in window, 768034 - 768451, 418 bps 
B D  S. cerevisiae  cgatttgtatattggatagaaatataattttttaaaaa---aagtaaactataagcagcctggaaagata
            sacPar  cggcttgtatactgagtaaaaatatggctttt--gaaa---aagcaaagtatgtgcagcttgaaaattta
            sacMik  tgttccttataccaggtagagacgccgtcctcaaagaatggaaagaaacactatacgatctgagtacaga
            sacKud  -----------------agagatgcagtcctc--aaaa---ggggaaaatgtatgtgatacgaaactgta
            sacBay  ca---------------ggcaagatgggctttcagtat---caaaaaggtatgg------tgaaagtata

     S. cerevisiae  agctagaccaattcttattgataaaaaa-ctaaagataaattcaaccga-------aaactaaaatctag
            sacPar  agctagaccaaatccaattaaaaaaaaaacctaggataaatttaactca-------aaactaagatcgaa
            sacMik  agatgaattcaattc--ttaaggaaaag-tttgagataaattcaactct-------agattgtaaatgag
            sacKud  attcaaattggaaaa--aaaaaaagaaa-gctgagacaattcccacttaaaaatccaaatcgagattaaa
            sacBay  aataa------------ataaaaacaaa-cctgaggtaaattcaacttaaaatccgaatttcaaactcaa

     S. cerevisiae  attgaaattaaaacaagaatgaatgaatggatcaaaggacctgaat-atgaaaaaaa-gtgcaggagaaa
            sacPar  gttgaaattaaaaaaag---gaatgaatggatcaaaggatctggat-atgaaaaaaaagtgtaggaggaa
            sacMik  attgaaaaaaaaaaaaa----------------------------t-ttcaagaaaa-------------
            sacKud  cttgaaattaaaaaaga--------aaccgatcgaaggac--gaatgatggagagaa-------acgaaa
            sacBay  actgaaaaatgaaaaag-------aaatggagggaaagat-----g-ataaaaaaaa------------c

     S. cerevisiae  gaaaattttctcgctgt-gg---------aaaataagccgaaaatccgttacct-ag--aggaaagaaag
            sacPar  gaaaattttctcgctgtagg---------aaaataagccgaaaatccgttagttgag--aagaaaaaaaa
            sacMik  ------tttctcgctat-ggccatgaggccaaataagccgaaaacccgctagttgaggaaagaaagaaaa
            sacKud  atattttttcttgcagagtg---------aaaataagccgaaaacccgctacgctag--ttgagaaaaga
            sacBay  gaaaattttcttgctacgtg---------aaatgaagccgaaaacctgttagttgag--------gaaaa

     S. cerevisiae  aaacgtgggaaaaaca-gggggcggtacccggc-ggcgtcaacttaacaggcataacctaggcgg--tag
            sacPar  gaacgtgtgaaaaaca-ggggtcggtacccggc-ggcgcca-ctccattggcataacctagacgg--tag
            sacMik  tgacatgctaaaaacaggggggcagtacccggcaagtgccaacaagaagtaggtaaactaagcaaaccaa
            sacKud  aaaaggtgagaaaacaggggggcggtacccgggaggtgtcacca--------ataagcaagtggg--cag
            sacBay  atacg-gcaaaaaata--ggggtggtacccgggaggtgtcacca--------ctaaaataagcac--caa

     S. cerevisiae  ttggcta------------------gttgg-caagcctggcaacatactccgatataggtaggaag----
            sacPar  ttgat---------------------------aggcctagcaacatactgcgacgtaaaacaacag----
            sacMik  tcaccag------------------acaggactagactagcagcaacctgcagcatataccaacg-----
            sacKud  agaacaa------------------g-----aaagggtagtagtaatttgagtcgcaacagagtaa--ac
            sacBay  gtagttgcagaagaagggcagtcttgtagaataagaatagcgtagccttgccgtgctagttgtaaggtaa

     S. cerevisiae  -agttagttataagaattgattgtcatacatacacatac--cttatacatatac
            sacPar  -tgttagatataagaattgattgtcatacatacacatacg-cttatacatatac
            sacMik  --gttagttataagacttaaatgtcatacatacacatacattctttacatatac
            sacKud  tagctagttataagaattgattgtcatacatatacatacg-tttatacatatat
            sacBay  aagctagctataagaattgattgtcatacatatatatacg-tatatacatatat

Alignment block 8 of 31 in window, 768452 - 768511, 60 bps 
B D  S. cerevisiae  ata--------taatttaagagaga--------------atagttcgtttcaatttttgctgtattgttc
            sacPar  ata--------taataaaagagaga----------gaaaatagttcgtttcagtttttgctatattgttc
            sacMik  ata--------tagttagagaggaaagagagaaaaaaaaatagttcgtttcag--cttgctgcactattc
            sacKud  atatctttttttaattagaggaaga-------gagaaaaagggttcgtttcggttttggctgtgttgttc
            sacBay  att---ttttttaggtagaggtggg----ggaaagagaaagagttcgtttcag---------catagttc
            sacKlu  ata-------aaagtcatagagagt--------------ggagtcc-tagcag--------agatt----

     S. cerevisiae  caggcagagc-gg
            sacPar  cggaaagagc-gc
            sacMik  cacgtagagc-gt
            sacKud  cagatgggacggc
            sacBay  c-------gt-gt
            sacKlu  -------------

Alignment block 9 of 31 in window, 768512 - 769023, 512 bps 
B D  S. cerevisiae  ttataattcaccggacttggcaacaacaattctagccttggtggcaccggaaggagaacccaaggactca
            sacPar  ttataattcgccggacttggcaacaacgattctagctttagtggcaccggaaggagaacccaaggattca
            sacMik  ttataattcaccggacttggcaacagaaattctggccttggtggcaccagaaggagaacccaaggactca
            sacKud  ttataattcaccggacttggcaacaacgattctagccttagtggaaccggaaggagaacccatggattca
            sacBay  ttataattcaccggacttggcaacgacgagtctagccttggtggcaccggaaggagaacccatagattcg
            sacCas  ttataattgaccagacttggcgatgacaattctagctctagtggcaccggatggggaacccatagtttca
            sacKlu  ttacaattcgccagacttggcaaccatcagtctagctctggtagcaccagaggaagaacccatggtttca

     S. cerevisiae  accttcttaacgatgtcgtaaccgtcaacaacttcaccaaagacaacatgcttaccgtccaaccatgggc
            sacPar  accttcttaacgatatcgtatccgtcaacaacttcaccaaaaacaacgtgcttgccgtccaaccatgggc
            sacMik  accttcttgacaatatcgtaaccgtcaactacttcaccaaagacaacgtgcttgccgtccaaccatgggc
            sacKud  accttcttgacaatgtcgtaaccgtcaacaacttcaccgaagacgacgtgcttgccgtccaaccatgggc
            sacBay  accttcttgacgatgtcgtaaccgtcgacgacttcaccgaagacgacgtgcttaccgtccaaccatgggc
            sacCas  atcttcttaacaatgtcgtaaccgtcagtgacttcaccgaagacgacatgcttaccatctaaccatggac
            sacKlu  atcttcttgacaatgtcgaaaccttcggaaacttcaccaaagacaacatgcttaccatctaaccatggac

     S. cerevisiae  atggaacggtggtgatgaagaattgagaaccgttggtgtttggaccggcgttggccatggacaacaaacc
            sacPar  atggaacggtggtgatgaaaaattgagaaccgttggtgtttggaccagcattggccatggacaacaaacc
            sacMik  atggaacagtggtgataaagaattgagatccgttagtgtttggaccagcattggccatggacaacaaacc
            sacKud  atggaacagtggtgatgaagaattgagaaccgttagtgtttgggccggcgttagccatggacaacaaacc
            sacBay  aaggaacggtggtgatgaagaattgggaaccgttggtgtttggaccggcgttggccatggacaacaaacc
            sacCas  atgggacggtagtgacgaagaattgagaaccgttagtgtttggaccagcgttagccatggacaacaaacc
            sacKlu  atggaacggtggtaatgaagaattgagaaccgttggtgtttggaccagcgttggccatggacaacaagcc

     S. cerevisiae  tggtctgtcgtggtgcttcttgaagttttcatctgggaatttgccaccgtagatagacttaccgccggta
            sacPar  tggtctgtcgtggtgcttcttgaagttttcgtctgggaacttgccaccgtaaatagacttaccgccggta
            sacMik  tggtctgtcatggtgcttcttaaagttttcgtctgggaacttgccaccgtaaatggacttaccgccggta
            sacKud  tggtctgtcgtggtgcctcttgaagttctcgtctgggaacttcccaccgtaaatagacttaccgccggta
            sacBay  tggtctgtcgtggtgcttcttgaagttttcatctgggaacttgccaccgtagatggacttaccaccggta
            sacCas  aggtctgtcgtgcttcttgacgaaattttcatcagggaacttgccaccgtagatggacttaccaccggta
            sacKlu  tggtctgtcgtgcttcttgacaaagttttcatctgggaacttgccaccgtagatggacttaccaccgaca

     S. cerevisiae  ccgttaccagcagtgaagtcaccaccttgcaacatgaagtctggaataactctgtggaatggagagccag
            sacPar  ccgttaccagcagtgaagtcaccaccttgcaacatgaagtctggaatgactctgtggaatggagagccag
            sacMik  ccgttaccagcagtaaagtcaccaccttgcaacataaagtctgggatgactctgtggaatggagagccag
            sacKud  ccgttaccagcggtgaagtcaccaccttgcaacatgaagtctgggatgactctgtggaatggagatccgg
            sacBay  ccgttaccggcggtgaagtcaccaccttgtagcatgaagtctggaatgactctgtggaatggggagccgg
            sacCas  ccattaccggcggtgaaatcaccaccttgcaacatgaaatctgggataactctgtggaatggagatccag
            sacKlu  ccggtaccatgggtgaagtcaccaccttgcaacatgaagtctgggataactctgtggaatggagagccgg

     S. cerevisiae  cgtagccgaatcccttttcaccggtacatagagctctgaagttttctgcagtctttgggactatgtcgtt
            sacPar  cgtagccgaatcccttttcaccagtacatagtgctctgaagttttcggcagtctttgggactatgtcgtt
            sacMik  catagccgaaacccttttcaccagtacatagcgctctgaagttttcagcagtctttgggactatgtcgtt
            sacKud  cgtagccgaagcccttttcaccagtacatagcgctctgaagttttcggcggtctttggaactacgtcgtt
            sacBay  cgaagccgaaccccttttcaccggtacatagcgctctgaagttctcggcggtctttgggactacgtcgtt
            sacCas  cgtaaccgaaccccttttcaccggtacataaggctctgaaattttcagcagtctttggtacaacgtcatt
            sacKlu  cgtaaccgaagcccttttcaccagtagccaaagctctgaagttttcggcggtctttggggtaacatcgtt

     S. cerevisiae  gtacaacttgaaaacgacacggccaattggttggccatcagcttcgacatcaaaatagacttgg---gac
            sacPar  gtacaacttgaaaacgacacggccaattggttgaccatcagcttcgacatcaaaatagacttgg---gac
            sacMik  gtacaacttgaaaacgacacggccaattggttggccatcagcttcgacatcaaaatagacttgg---gac
            sacKud  gtacaacttgaaaacgacacggccaactggttgaccatcagcttcgacatcaaaatagacttgg---gac
            sacBay  gtatagcttgaaaacgacacggccaattggttggccatcagcttcgacatcaaaataaacttgg---gac
            sacCas  gtacaattggaaagtgatacgacctaaagcttggccgtcggcttcgacgtcaaagtataccttgttagac
            sacKlu  gtacaatttgaaaacgatacggcccaatggttggccatcagcttcaacgtcgaagaaaacttta---gac

     S. cerevisiae  atgg---ta-gtattagcggttgagtttg
            sacPar  atgg---ta-atatttgcggttgagtt-g
            sacMik  atgg---ta-gtatttgcgattgagtt-a
            sacKud  atgt---ta-aaatttgcgattgggtg-a
            sacBay  atgg---taggtgttttcggttgagtt-g
            sacCas  attgatata-tgatttttgtttgg-----
            sacKlu  atga---tt-gattatttagtttg-----

Alignment block 10 of 31 in window, 769024 - 769396, 373 bps 
B D  S. cerevisiae  gattgagttgagatattaaattcaagacaaatc----ttaatataacctatctagcgaaaaaggaggaac
            sacPar  aattgagttgagatactaa-ttcaagacagatagataccaatataacctaattaacaaaaaaggagggtg
            sacMik  ggttaagttgagatgctactttcaagtcagacc--taagaacacagtgtaacaagggggaaaagaacctc
            sacKud  gattgagttgacgatttag-accaaaagagaga--gaccgaaaaaagagaattaactgatgaagagcaaa
            sacBay  ggttgatttgaggaattga-gtcaaa---------tatgaaaagagcaagacggacaggagggaggaaaa

     S. cerevisiae  aaatgatgacgacagtggcaaaaggatagaaggctcaagagaatacgtttatatttatacagggaatcga
            sacPar  aa----tgtcaacagtggcaaaaggataagaggctcaagagaatacgtttatatttatacagggaatcga
            sacMik  ta---acatttatagtggcaaaagaataaaacgctcaagagaatacgcttatatttatacagggaatcga
            sacKud  aatgaaggccaacagtggcaagagaataagacgctcaagagaatccgtttatatttatacagcgaattga
            sacBay  ga---agctctatagcaataaaagaacgaacgaacaaagagaatgcacttatatttatacagacagacag

     S. cerevisiae  aca-gacactt-gt----------cagcgtaagcccaaccaaagtgag--------aagggccacggcca
            sacPar  acaagacactttgt----------cagcgtaagtccaaccgaagtgag--------aagggccaccgcca
            sacMik  aca-gatacttcgt----------cagcataagcccaaccaaatggaa--------aagggccacggtca
            sacKud  aca-gacactttgtcagaagaaggcagcggaagtccaaccaaataga---------aaggcccacggcca
            sacBay  aca-g-------ga----------cagcgtaagcccaacagaacctagggccgttccgtggccgtggccg

     S. cerevisiae  ttaccagagacagaaatgagtggaagcgcgtggaagcccgtctagcaga--ggaagaa----attcga-g
            sacPar  ttaccagagacggaaatgagtggaagcgcgtggaaggccgtctaacagaaaggaagaa----attcga-g
            sacMik  ttaccagagacggaaatgtgtggaacagcgtagaagcccgtctaacaaa-aagaagaa----atctga-g
            sacKud  ttactgggaacgggaacgtgtggaagcctgtggaagtctgcctagcagaaaggaagaatccgaggcga-g
            sacBay  tggccagggacggaagcgcgtggaagcacgtggaagtccgcctggcggg-------------atcctacg

     S. cerevisiae  acgaggcgggac----------gtggcgggctgg--gcgcccc-agaaaataccgtagaggacgaggggg
            sacPar  acgaggcgggac----------gtggcgggctag--gcgcccccagagaaggcagtagagagggaggggg
            sacMik  acgaggcgggag----------gctacgggctgg----acccc-agaaaacgtcgtaggggaggaggggg
            sacKud  gcgaggcgggac----------taggcgagctagagggggccc-agaggaggccgtagaggacgagggg-
            sacBay  cccaggcgaggcttccgcctgggcgatgggcgag----agcct-ag----------ggggaacgaggggg

     S. cerevisiae  gcggtg-gaaacccctaaggggcgaaag--gcgggtaacatcta-acagcgacgggtaagcatattgcg-
            sacPar  -cagta-ggaacccctaaggggcgcagg--gcgggtaacagtaatattggggcgggtaagagtattgcg-
            sacMik  aaagcg-ggtacccttaaggggcgcaag-agcgggtaatagct--atccgggcgggtaagaggcttgcg-
            sacKud  -cggcgagaaacccctaaggggcgcaag-aacgggtaacgagc--tcaagggcgggtaaggatttccag-
            sacBay  -cggcg-g--acccctaaggggcgcgggcggcgggtaagcgc---gcgcgggcgggtaag---cttccgt

     S. cerevisiae  gg
            sacPar  gg
            sacMik  g-
            sacKud  ga
            sacBay  ga

Alignment block 11 of 31 in window, 769397 - 769511, 115 bps 
B D  S. cerevisiae  tgtaaaa----tttttcattt-----cgagctta--------cttcaa---gatgatgcatatgtttaaa
            sacPar  tgtaaaa----attttcattt-----cgagctta--------ttacaa---gatgatgcatatgtttaaa
            sacMik  ---aaaatttttttttcattt-----caagctcgcttattctctacaagatgatgatgtatatgcttaac
            sacKud  cgtaaaa----tttttcatttacgaaccaactgg--------gtacaagatgatgatgcatgtggtgaag
            sacBay  tgtaaaa----tttttcatc------cgagcttg--------gtacaa---gatgaggtatgtatgcaag
            sacCas  tggaaaa----tttttcatgt-----tgaaagca--------tctcat-----------ctctattgaag

     S. cerevisiae  tcacattgattatagaggtctcatcggtaaggcagagcagaactgg----agacgtctgaggtaagatt
            sacPar  gcacaacga----aaacatctcatcggtaaggtggagcggagctagctacagagaccaagagtataata
            sacMik  gcaaaacaa-aaaacagatgtcagcggttcagtagagcagtactaa----ataagtcaggaaatta---
            sacKud  caaaaacag--acacacatctcatcggtaggatacaccagggctgaaaagaggactcaaaa---taata
            sacBay  acagaacat----------ctcatcgg-gcggtacagcacagcaca----gcacagcacag--------
            sacCas  aacaatcca---------tatcagc-------tacggcagcaccag----------caaca--------

Alignment block 12 of 31 in window, 769512 - 769829, 318 bps 
B D  S. cerevisiae  tcgataatacaagatgatgaaaggttctagaagaacaggtaataacactgctaccacgctaaacacgcct
            sacPar  tcaataatatatgatgatgaaaggttctagaagaacaggcaataacgcggctactacgctaaacactcct
            sacMik  -----aacataccatgatgaaaggttccaggagaacgggcaacaacgcgtctactacgctaaacacacct
            sacKud  aaaaaagtgcaatatgatgaaaggttcaagaagaacgggcaacaacatggccaccacgctcaacaccccc
            sacBay  -----cacacgatatgatgaaaggttccagaagagcaggcaataactcggtcaccacactcaacaccccc
            sacCas  -------------atgttcaagggcactagaagagcagctttcaaccaatccacacctctgaatacccca
            sacKlu  tcgatagaaca--atgatcagaggctctagaagaccagctaa---------cactacattaaatactcct

     S. cerevisiae  gtggtcattcatgccacgcagctgccccaacatgtctctactgacgaggttcttcaatttctggagagct
            sacPar  gtggtcattcatgccacgcagctgccccaacatgtttctactgacgaggtccttcaatttctggaggggt
            sacMik  gtggttattcatgcgacgcagttgccccaacatgtctctactgatgaggtcctacaatttcttgagggct
            sacKud  gtggtcatccatgccacgcagctgccccaacacgtctctactgacgaggtcctgcaattcctggagggct
            sacBay  gtggtcatccacactacacaactgccccagcacgtctccacggacgaggtcctgcagttcctggagggct
            sacCas  gtggttattcacaccaccgcgcaaccacaacatgtcaacaaggatgacgttctgaaattcctcgataaat
            sacKlu  gtgacactacatattgtagataaggcacatcatgtttccaaggacgaagctttgcaattcctggccaagt

     S. cerevisiae  ttatcgatgagaaggagaacattattgatagtactaccatgaatacca--------tcagc----ggcaa
            sacPar  ttattaatgagaaggaaaatactatcgatagcaccacgatgaacacca--------tcagc----ggcag
            sacMik  tcatcaacgagaaggaaaacgttatcgatagcactacggtgaatacca--------tcagc----ggcag
            sacKud  ttattaacgagaaggagaacatcattgatagcaccacgatgaacgtcg--------tcagc----gacaa
            sacBay  tcatcaacgagaaggagagcatcatcgacagcaccacgatgaacacca--------tcaac----gtcaa
            sacCas  tcatcacagagaaggaaaatctaatacagaacagtacagtggccctgggc------tcggcagaaggtac
            sacKlu  tcattaacgagaaaga-------atccatgtcagaa--atagacaccagcggtttgtcctc----atcca

     S. cerevisiae  cgccgctgacgcggacgct---gccgctgtggctaacaccagcctcaacatcgacaccaacctttccagc
            sacPar  cgccgctgacgcagacgct---accgctgtggctaaccccggtctcaacatggatactaacctttccagc
            sacMik  cgccgctgatggcgacgtg---acagctgtagtaaaccctagccttaacatggatactaaccttttcagc
            sacKud  cgccgctggcgcggacgtt---gtccctgcggtcaacgccagcctaagcatggatactaacctgtccagc
            sacBay  cgccgcggacgcggacgttggcgccgctgccgccaaccccggtctgaacatggacaccaacctgtccagc
            sacCas  agccgctgtcgggggtgat---------gctgccaccgcattgctcagtatagacaccactttatccagc
            sacKlu  catcacaagaagtaacatc-----aggtggagtctccagcgatacaaacgtagataccagtttatccagc

     S. cerevisiae  tccatatcgcaattaaagaggattcagagagacttcaagggtctgccacccgc
            sacPar  tccatatcgcaattgaagagaattcagagagacttcaagggtctgccacccgc
            sacMik  tccatatcacaactaaagagaatccagagagatttcaagggtctaccaccagc
            sacKud  tccatatcacagcttaagaggatccagagagacttcaagggtctgccccccgc
            sacBay  tccatatcacaactgaagcgaatccagagagatttcaagggcctgcctcccgc
            sacCas  tcattatcgcaattgaagagattacagagagatttcaaaggtttgcctcctgc
            sacKlu  tcactggcacaattaaagagggttgaaagagatttcaaaggtttgcctccagc

Alignment block 13 of 31 in window, 769830 - 769951, 122 bps 
B D  S. cerevisiae  ccaagacttttccg---ccgcgcccattcaggtctccacgacggagaagaaggagacaagca-----tag
            sacPar  ccaagacttctctg---ccgcgcccattcaggtctccacggcggagaagaaggaaactagca-----tag
            sacMik  acaagacttctctt---ctgcgcccatccaggtctccacagcggacaaaaacgagaccaacg-----tcg
            sacKud  ccaagacttctccg---ccgcaccgattcaggtcaccacggccggtaagaacgacgccagtg-----tcg
            sacBay  ccaggacttctctg---ccgcccccatccacgtctccaccgccgctaaagacgagactagcg-----tcg
            sacCas  ttcaatgatcgccgaatctgtatccagtga---------aacaaacaaggaaagtactggcgaaagttca

     S. cerevisiae  gtgtt----agtgccaccggcggcaagaaaactacattcgccgatgagtgagacctgtagcagc
            sacPar  gtgtc----agtgccaccggcggcaagaaaaccacattcgccgatgaataagggttgtggcaac
            sacMik  gtgtc----agtgccaccggtggcaagaaaaccactttcgctgatgaatgagcgctgtc-cgtc
            sacKud  gcgtc----agtgccactggtggcaagaaaaccacattcgccgacgaatgagccctgtg-aaac
            sacBay  gcgtc----agtgccaccggcggcaagaagactacgttcgctgacgagtaag--------gcgc
            sacCas  gtgacaaagagtgtcactggaggaacaaagaaaacattcagcgatgaggaataaattttgaaat

Alignment block 14 of 31 in window, 769952 - 770322, 371 bps 
B D  S. cerevisiae  agccttgcccttt-tacacaaaatcctacatatccattcacatacgccttct--gtatactacaatatat
            sacPar  aatcttgctcttt--acacaaaatcctacgtatccattcacgtacgccttctttgtatactataatatat
            sacMik  tgtcttcctgctc--atacaagatcttacatatccattcacatacgcctttt--gtatactacaatatat
            sacKud  gcgcttgtcct----acttaaaatcttacatatccatccacatacgccttct--gtatactacaatatat
            sacBay  gctctagccccttttacatagcaccctacatatccgcccacatacacattct--gtatactacaatatat

     S. cerevisiae  g---------------catatcagttagaattctgacatc-tg--------aacgtcttcagtgcttttt
            sacPar  g---------------catatcagttaaaatcccaataccatg--------aacgtatcca----ctatt
            sacMik  gcatatcataacatatcatatcatctcgaatcctgatgccgtagtagccgcagtgtgctca----ctatt
            sacKud  g---------------catgccagttagaattctgacactgta--------agc-----------ttctt
            sacBay  g---------------cataccagttccagtcctgacact-ca--------aggagcctct----ctctt

     S. cerevisiae  tt-tctctt----------tctttccagatccaaatatcaccggcagcggcctcaaag---tacccggtt
            sacPar  tt-tctctt----------ccttttcatattcattt-tcaccggcagcggcctcaaag---tacccgctt
            sacMik  ttctctctt----------tctcttcagattcaata-tcaccggcagcggcctcagaa---tagtcgctt
            sacKud  tt-tctctt----------tcttttcttgttcaaat-tcaccggcagcggcctcgaagaactgcccgctt
            sacBay  tc-tctcttttaccgaccgtcttctgattttctgat-tcaccggcagcggcctcgaaa---ctgccgttt

     S. cerevisiae  ttctttttttt-cacactttttttt----------tttccaaatcatgttgtcaagca-ttgattgactc
            sacPar  ttcttttttt--cacactttttttt--------------cacactacgttgtcaggca-ttgattgactc
            sacMik  tttcttttttt-cacactttttttt-------cttttttcacagta---tgtcaaaca-catattgactc
            sacKud  tttctttttc--cacatttttttct---------tcttccacacta---cgtcaagca-ttgattgactc
            sacBay  ttttttttttcacacactttttttttttttctctttcttctaccta---cgtcaagcaggtgattgactc

     S. cerevisiae  attttcctctgac-t---actaccagttcaaaatgttagagaaaaatagaaaagcagaaaaaataaataa
            sacPar  attttcgtgtgat-t---actttcagttcaaaatatcaaagaaaaatcgaaaagcagaaaaaataaataa
            sacMik  a-tttcctctgatgt---aataccagctcgaaatgtcaaagaaaaatagaaaagcagagaaa--aaataa
            sacKud  attttcctctgat-tacaactaccaactcgaaatgccaaaaaaaattagaaaagcaggaaaa--aaataa
            sacBay  a-ttttctttgat-t---accaccaattcgaattgccaaaaaataaga-aaaagtag-------aaataa

     S. cerevisiae  aagaa--ccatatttatttgttcat--tcactttcgagtataaaattaaatcttgttt-ttcttg-tttt
            sacPar  aagaa--cagtacttatttattcat--ttacttttgagcacataattaaatcttgctc-ttcttg-tttt
            sacMik  aagag--ctgtaagtatttagtcat--tgcgttttgaacacaggattaaatcttgtcc-ttcttgctttt
            sacKud  aagtggcccattttgacttagtgattatggctttcggacactagctttaatcttccccgttcttg-tttt
            sacBay  aaact--tgctatttatctagtcattttatcttttcggcagaggcttaattcttacct-ttttta-atgt

     S. cerevisiae  cacttaa--gtacaca
            sacPar  catttaa--atacaca
            sacMik  ctctcaa--acatata
            sacKud  cacataggtgtatata
            sacBay  aacgtat--ataggtg

Alignment block 15 of 31 in window, 770323 - 770356, 34 bps 
B D  S. cerevisiae  tacaaattaaat-tgtag---a--aataaagcgttctaaa
            sacPar  cgcaaactaaat-aataacaaa--agaaaagctttctaat
            sacMik  cgaaagttaaat-aata----a--gtaaaagattttaaag
            sacKud  cgcaaaataaa--aacaaccaa--aaaagtacacttaaaa
            sacBay  aagaaaaaaaaa-aataatcaattaacacatctttttgta
            sacKlu  tacaaaacaaatcaataaacc-------------------

Alignment block 16 of 31 in window, 770357 - 770502, 146 bps 
B D  S. cerevisiae  atggctggaaagaaaattgctggtgttttgggtgctactggttccgttggtcaacgtttcattctgttgt
            sacPar  atggcaggaaagaaggttgctggtgttttaggtgctactggttccgttggtcaacgtttcattcttttgt
            sacMik  atggctggtaagaagattgctggtgtgttaggtgctactggttctgtgggtcaacgtttcatcctcttgt
            sacKud  atggctggaaagaagattgctggtgtcttgggcgccactggttccgtgggccaacgtttcatccttctgt
            sacBay  atggctggaaagaaaattgctggtgtcttgggtgccacaggttccgtgggtcaacgtttcatccttctgt
            sacCas  gtggctcaaaagaaaattgctggtgtcctaggtgcaaccgggtccgttggtcaacgtttcatcttattat
            sacKlu  atgtctcaaaagaaaatcgctggtgttctaggtgccacgggctctgttggccaacgtttcattttactac

     S. cerevisiae  tggcaaatcaccctcatttcgaactgaaagttcttggtgcctcttctagatcagctggcaagaaatacgt
            sacPar  tggccgatcaccctcatttcgaactgaaagttcttggggcctcttctagatcagcaggtaagaaatacgt
            sacMik  tggctgatcaccctcatttcgaactaaaagttcttggagcttcttctagatctgccggtaaaaaatacgt
            sacKud  tggctgaccatcctcacttcgagttgaaggttcttggggcctcttccagatctgccggtaagaaatacgt
            sacBay  tggctgaccatcctcacttcgaattgaaagtccttggggcctcctccagatctgccggtaagaagtacat
            sacCas  tggccgaacatccaagtttcgaacttagagtcttgggtgcatctccaagatccgcaggtaagaaatatat
            sacKlu  tggccaaccacccagactttgaactaaaggtcttgggtgcttccccaagatctgctggtaagaagtacat

     S. cerevisiae  tgacgc
            sacPar  tgatgc
            sacMik  cgatgc
            sacKud  cgacgc
            sacBay  cgacgc
            sacCas  tgatgc
            sacKlu  cgatgc

Alignment block 17 of 31 in window, 770503 - 770745, 243 bps 
B D  S. cerevisiae  tgtgaactggaagcaaaccgatttgctaccggaatctgctaccgatattattgtttccgaatgtaaatct
            sacPar  tgtgaactggaagcaaaccgatttgcttccagaatccgctaccgacatcgttgtttccgaatgtaaatct
            sacMik  agtgaactggaagcaaactgatttgctgccagagtccgctaccgatatcgttgtttctgaatgtaaatcc
            sacBay  cgtcaactggaaacagaccgacttgctaccagagtccgccaacgatatcgtcgtttccgaatgtaaatcc
            sacCas  agtcaactggaagcaaactgatttattgccaactatggctgaagatatcattgtcactgaatgtaaatca
            sacKlu  tgttaactggaagcaaaccgatctactaccggaatttgctaaggacattattgttaccgagtgcacatcc

     S. cerevisiae  gaattctttaaagagtgtgacatcgtcttttccggattggatgctgactatgctggcgctatcgaaaagg
            sacPar  gaattcttcaaagaatgtgacatagtcttttcgggattggacgctgactatgctggtgcgattgaaaagg
            sacMik  gaatacttcaaagagtgtgacattgtattctctggattggatgctgactacgctggtgccatcgagaagg
            sacBay  gaattcttcaaagactgtgacatcgtcttttctgggttggacgccgactacgctggttccatcgaaaagg
            sacCas  gaatttttcaaagattgtgatattgtcttctctgggttagatgctgattacgctggtgccattgaaaagg
            sacKlu  gatgcttttaagcaatgtgatgttgttttctccggtttagatgcggattatgccggtccaattgagaagg

     S. cerevisiae  aattcatggaagctggtatcgccattgtttccaatgccaagaattatagaagagaacaagatgtgccatt
            sacPar  aattcatggaagccggcatcgccattgtctccaatgccaaaaattacagaagggaacaagatgtaccatt
            sacMik  aattcatggaggccggtatcgctatcgtatccaatgctaagaattacagaagggaacaagatgtgccatt
            sacBay  aattcatggaagccggcatccccatcgtttccaacgccaagaactacagaagagaacaagacgtgccatt
            sacCas  aattcgtcgaagctggtcttgccgtcgtctctaatgccaagaactacagaagagaagaagacgtcccatt
            sacKlu  aatttgtcgaagccggtctagctgttatttccaacgccaagaactacagaagagaggcagacgtaccatt

     S. cerevisiae  gattgttcctgttgtcaatcctgagcatttgga
            sacPar  gatcgttcctgtagtcaaccctgagcatttaga
            sacMik  gattgttcctgttgtcaaccctgaacatttgga
            sacBay  gatcgtccccgtcgtcaaccctgaacatttgga
            sacCas  attggtccctatcgttaacccagaacatttgga
            sacKlu  ggtcgttccaatcgttaatcctgaacacatgga

Alignment block 18 of 31 in window, 770746 - 771454, 709 bps 
B D  S. cerevisiae  tattgtagctcaaaagcttgacaccgccaaggctcaaggtaagccaagaccagggttcattatctgtatt
            sacPar  tattgtggctcagaagctagaaaccgccaaggttcaaggcaagccaagaccaggtttcatcatctgtatt
            sacMik  tattgttgctcaaaagctagagactgccaaggctcaaggaaagtcaagaccagggtttatcatctgtatt
            sacKud  tattgtttctca-aagctggaaactgccaaggcgctgggcaagccaagaccggggttcatcatttgtatt
            sacBay  cattgttgcccaaaagctggagaccgccaaggcccaaggtaagccaagaccaggtttcatcatctgtatc
            sacCas  tatcgtttctaacaagttggaaaaggcaaagagtgaaggtaagtctaaaccagggttcattgtctgtatc
            sacKlu  tatgatcgctactaagttggaaaatgcaaaggccgctggtgtgtctaagccgggttttatcgtttgtatc

     S. cerevisiae  tccaattgttccactgcaggtttggttgcaccattgaagcctttgattgaaaaattcggtcctattgatg
            sacPar  tccaattgttccactgcaggcttagttgcgccattgaagcctttaattgaaaaattcggtcctattgacg
            sacMik  tccaactgttctaccgcaggcttggtcgcgccattgaaacctttggtcgaaaaattcggtcctattgatg
            sacKud  tccaactgctccactgctggcttagtcgccccattgaaacctttgattgaaaaattcggccctattgacg
            sacBay  tccaactgttccaccgccggcctcgtcgccccattgaagcctttgatcgagaagttcggtcctatcgacg
            sacCas  tccaattgttccaccgcaggtttggttgctccattgaaaccattagtggaaaaattcggtccaattgatg
            sacKlu  tccaactgttctaccgctgggctggtggctccattgaaacctttggttgaaaagttcggtcctattgacg

     S. cerevisiae  ctttgaccactactactttgcaagcaatctcaggtgctggtttctccccaggtgtaccaggtattgatat
            sacPar  ccttgactaccactactttgcaagcaatctcaggtgcaggtttctctccaggtgtgccaggtattgatat
            sacMik  ccttgaccaccgctactttgcaagcaatttcaggtgcgggtttctctccaggtgtgccaggtattgatat
            sacKud  ccttgactaccaccactttgcaagccatctcaggtgcaggtttctcgccaggtgtgccgggcatcgacat
            sacBay  ccttgaccaccaccactttgcaagccatctccggtgccggtttctcccccggtgtcccaggtatcgacat
            sacCas  ctttgactactactactctacaagccatctctggtgccgggttttcaccaggtgtcccaggtatcgatat
            sacKlu  cattgacaactactactttgcaagctatttccggtgccgggttctctccaggtgtcccaggtattgatat

     S. cerevisiae  tctagacaatattattccatacattggtggtgaagaagacaagatggaatgggagaccaagaaaatcttg
            sacPar  cctagacaatatcattccatacattggtggtgaagaagacaagatggaatgggaaaccaagaaaatcttg
            sacMik  cctagacaatatcatcccatacattggtggtgaagaagacaagatggaatgggaaaccaagaaaattttg
            sacKud  tctggacaacatcatcccatacattggtggcgaagaagacaagatggaatgggaaacgaagaaaattttg
            sacBay  cttagacaacatcatcccatacatcggtggtgaagaagacaagatggaatgggaaaccaagaaaatcttg
            sacCas  cttagataacattattccatacattggtggtgaagaagacaagatggaatgggaaaccaagaagatcttg
            sacKlu  tttggacaacattatcccatacattggtggcgaagaagataagatggaatgggaaaccaagaagattttg

     S. cerevisiae  gctccattagcagaagacaagacacacgtcaaactattgactccagaagaaatcaaagtctctgctcaat
            sacPar  gccccattggcagaagacaaaactcacgtcaaattattgactccagaagaaatcaaagtctctgctcaat
            sacMik  gctccattgtcagaagacaggactcatgtcaaactcttgacttcagaagaaattaaagtctctgctcaat
            sacKud  gccccattggccgaagacaagactcacgtcaaactgttgaccccagaggaaatcaaggtctctgctcaat
            sacBay  gccccattgtccgaagacaagacccaagtcaaactattgacccctgaagaaatcaaggtctctgctcaat
            sacCas  ggttccattgcagaggataagactcacattaaattgttaactccagaagaaattaaggtttctgcccaat
            sacKlu  ggttctctaaatcaagacaactcaagtgtccaactattgtcagatgatgagattaaggtttctgctcaat

     S. cerevisiae  gtaacagagtcgctgtttccgatgggcacaccgaatgtatctctttgaggttcaagaacagacctgctcc
            sacPar  gtaacagagtcgctgtctctgacggccacaccgaatgtatctctttgagattcaagaatagacctgctcc
            sacMik  gtaatagagttgccgtctctgacggtcacaccgaatgcatctctttgagattcaagaatagacctgctcc
            sacKud  gtaacagagtggccgtctctgacggtcacacagaatgtatctctttgagattcaagaacagacctgctcc
            sacBay  gtaacagagtggctgtttctgacggtcacacagaatgtatctctttgagattcaaaaacagacctgctcc
            sacCas  gtaacagagtggctgtctctgatggtcacaccgaatgcatctctcttagatttaaaaatagaccagctcc
            sacKlu  gtaacagagttgccgtttctgatggtcacactgaatgtatctctttgagattcaagaaccaacctgcccc

     S. cerevisiae  atccgtcgagcaagtcaagacatgcctaaaagaatacgtctgcgatgcctacaaattaggctgtcattct
            sacPar  atcagtcgaacaagtcaagacatgtctgaaagaatacgtctgtgacgcttacaaattgggctgccattct
            sacMik  atctgtcgaacaagtcaagacatgtctaaaagaatacgtttgtgacgcttacaaattaggttgtcattct
            sacKud  atccgtcgaagaactcaagacatgtctaaaagaatacgtctgtgacgcgtacaaattgggttgccattcc
            sacBay  atccgtcgagcaagtcaagacatgtctaagagaatacgtttgtgacgcttacaagttgggctgccattcc
            sacCas  atctgtcgatgcagttaagcaatgtttaagagattacgtttgtgatgccttcaagttgggttgtcattct
            sacKlu  atctgtcgaagaggtcaagcaatgcctaagagattacgtctgtgatgcaaccaaactaggttgtcattcc

     S. cerevisiae  gctccaaagcaaactattcatgttttggaacaaccagacagacctcaaccaaggttggacaggaacagag
            sacPar  gctccaaaacaaactattcatgttttggaacagcctgatagacctcaaccaagattggacagaaacagag
            sacMik  gctccaaaacaaacaatccatgttttagaacaacctgatagaccacaaccaaggttggacagaaacaggg
            sacKud  gctccaaagcaaaccatccatgtcttggaacagcctgatagaccacaaccaagattggacaggaacagag
            sacBay  gctccaaagcaaaccatccacgtcttggaccaagccgatagaccacaaccaagattagacagaaacagag
            sacCas  gctccaaagcaaactattcacgtcttggaacaaaatgacagaccacaaccaagattggacagaaatagag
            sacKlu  gctccagaacagactatccacgtcttggaacagagcgacagaccacaaccaagattggacaggaacaggg

     S. cerevisiae  acagcggttacggtgtttccgttggtagaatcagagaagacccattgttagatttcaaaatggttgtcct
            sacPar  acagcggttacggtgtctctgttggtagaatcagagaagacccattgttggacttcaaaatggttgtact
            sacMik  acagtggttacggtgtctctgtcggtagaatcagagaggatccattgttggacttcaaaatggttgttct
            sacKud  acaatggttacggtgtctccgtcggtagaatcagagaagacccattgttggatttcaaaatggtcgtcct
            sacBay  acaacggttacggtgtctccgtcggtagaatcagagaagacccattgttggacttcaagatggtcgttct
            sacCas  acgccggttacggtgtttctgttggtagaattagagaagaccctgttttagatttcaagatggttgtttt
            sacKlu  acaacggttacggtgttagtgttggtagaatcagagaagatcctgttctagacttcaagatggttgtgct

     S. cerevisiae  ttcccacaacaccattattggtgccgctggttctggtgtcttgattgccgaaatcttactagcaagaaac
            sacPar  ttcccacaacaccattattggtgccgctggttctggtgtcttgattgccgaaattttgctagcaagaaac
            sacMik  atcccacaacaccattatcggtgccgccggttctggtgttttgattgccgaaatcttacttgccagaaac
            sacKud  ttcacataacaccatcatcggtgccgctggttctggtgttttgattgccgaaattttactagcaagaaac
            sacBay  atcccacaacactatcatcggtgccgctgggtctggtgtcttgattgccgaaatcttattggcaagaaac
            sacCas  atctcataacaccatcattggtgctgctggtgcaggtgttttaattgctgaaatcttgttggctagaaat
            sacKlu  atctcacaacaccgttatcggtgctgccggtgctggtatcttgattgctgaaatcttgttggccaggaac

     S. cerevisiae  ttgatttaa
            sacPar  ttgatttaa
            sacMik  ttgatctaa
            sacKud  ttgatctaa
            sacBay  ttgatctaa
            sacCas  atgatctaa
            sacKlu  ttgatctaa

Alignment block 19 of 31 in window, 771455 - 772191, 737 bps 
B D  S. cerevisiae  ggaaaa---------attgcaaag-ttatg------tcttcatcttactttttt--ttcgaacga-----
            sacPar  gaagaa---------attgcgaag-ttatg------tcctcatcttgctttatt--ttcgaacga-----
            sacMik  gaaaaa--------gactgcaaag-ttataagtccttcctaatctaacttttct--ctccaacga-----
            sacKud  gaggaa---------gctgcaaagtttgtg------tctttgtcttcttttttt--ct----caa-----
            sacBay  ggaatg---------acctgcaag-ttacc------tccctctctt-ttttctc--cccgaatca-----
            sacCas  tgataatagaaatgtggcgaacaa-ttgta------tcataccctcttttctttaattttaatgatgact

     S. cerevisiae  ---acaaa--aaatacaaaacaagtaa-ataagtata----tgtgtatgaatatacg-tat-------aa
            sacPar  ---acaaa--aaatacaaaacaagtaa-ataaatata----tatgtatgaatatacg-tat-------aa
            sacMik  ---acaa---------aaaacaagtaa-ataatt----------------atgtacg-tat-------aa
            sacKud  ---acaaa--ataaacgaaacaagtaa-ataaa-aaa----tatatatgaatatacg-tac-------ag
            sacBay  ---acgaa--------caaacaagtaa-atata---a----tatatatgaatatacg-tatgcgtataga
            sacCas  ttgataaattttatatagtatacgtaacataataatagcgttaagtaccattatgttctaa-------aa

     S. cerevisiae  taat--ttgaaacgtagatct---------ttattattagtt--------tcttgagtttcgagaattcg
            sacPar  taat--ttgaaacgtagatct---------ttattatcaattatgaat-tttttgaatttcgagaatccg
            sacMik  tagc--tcgaaacttaggttt---------ctattattaattatttga-gttttgggttttgggcatcag
            sacKud  taat--ttgaagcctagtttt--------attattacaaatc--------ctttgagcttcgagcattct
            sacBay  taat--ttgaaac-tagattt--------attattaccaaat--------cctctagctctcgccctctt
            sacCas  gaatacacaaaacgaaaaacttttctggaactgctgctaatggaggatcgtgttagggttcaaac-----

     S. cerevisiae  ----tgattcaatgttcattattttt---tcttt-tcttttcttttcaatagtataa----tac-caagc
            sacPar  ----taattcaatgtttattaatttc---tcttcctctttttctttcagtataatag----tacacaaat
            sacMik  ----tgattccatgcttgttagcttt---tc----tcttttttcttcattttcaaagagcatac-ccagc
            sacKud  cgcccgattcgat-----ttgttttc---ttttg-tttttttcgctta-------ag----tac------
            sacBay  -------tccggag---attttttac---tcatg-tctgcttt------------------tac-cacat
            sacCas  ----taattcaaactttgtagtatttaaagtatt-cattttccctctgattatggaa----tac-taaag

     S. cerevisiae  ctt----tattatttttcttccacttc---------aagaaaaggtaattctctcgctgacccaagaaaa
            sacPar  cttgctttattaattttcttccacttc---------aaaaaaaggtaattc----gctgacccaagaaaa
            sacMik  ctaactttattgattttcttactctac---------aaagaaaggtaattt----gctgacccaagaaaa
            sacKud  --------------tttctttcatttc---------aaaaaaacgta-----------------------
            sacBay  ctt--cttacttgtttttctgcatttttaagagaaaaaaaaaaggtaattt--tagcttcgtgaaagaag
            sacCas  tgt-----------ttttttgccgcca---------tagtaaatttcattg------ttttcaaaaaaaa

     S. cerevisiae  aa--gctaaacagagagcgtagtgatgcatacaaacaactcatttacatgggaaaaaaaaggagaga---
            sacPar  aaaagctaaacagag--cgtattaatgcatatataaaactcatttacacag-agacagaagcaaaga---
            sacMik  aa-agttaaacaaag--tgtttta---catccgcataattcaacggcaga--caaagaaaggaaata---
            sacKud  -------aaacgaag--tgcagtggtacattcacgcagccctattgcaca--tgagaaaag---------
            sacBay  aaaaatataacaaag--cgtaatg-----tgtatacagctcgattgaagat-aaaaaaaaaaaaagaaag
            sacCas  aa-----tagtgaag--ccggttaacgtttctgaaca------ttata----tgaaaagaccggtta---

     S. cerevisiae  --------ggaaa---------gcagcagacatatct----caaagctgggatacactcacaatagcact
            sacPar  --------ggaaa---------gcagcagatatatc-----caaggttaggatacacttataacagctct
            sacMik  --------gggaa---------acagcagataaatct----caagactggaatacactaaccatatttct
            sacKud  ----------------------accgctgataaatct----caagaccgggagaaactcacaataactct
            sacBay  ataagaaggaaag---------gtagcgaatacgtct----caagactgggagatactcactatagcttt
            sacCas  --------cgaaattgaatcttgaaacagactcaactggcagaagaaaaaga-acgcacacattgact-t

     S. cerevisiae  gttgg-------tactcatttcaggtaatactcttgg-aaggtatcccttaaatgaacacatcatttggc
            sacPar  gttgg-------tactcatttcaggtaatactcttgg-aaggcgtccct-aaatgaacacatcatttggc
            sacMik  gttggcc----attttcgtttccagtaatactcttggaaaggtgattctcaaatgaacacaacatttggc
            sacKud  gttgg-------tcctcactttaggtagtttttttgg-aaggtgattcttaaatgaacacaacattcggc
            sacBay  gtcag-------------------gtacaactctcgg-aaggtgatccttaaatgaacaccagtttcggc
            sacCas  gctagacaaatataatccattctgaaaggataccagg-gacgttatcc------gatgactacctttggg

     S. cerevisiae  tcagtagtgccttccactaatttcaattttttcaaagggcatggaaataatgataacactagtgccaata
            sacPar  tccgtagtgccttccactaatttcgattttttcaaagggcctgcaaataatgatagtactagtaacaatg
            sacMik  tcgatagtgccttccactaattttgatttttttaatttgcccagtaataatgataatactagtacaaatg
            sacKud  tcaatagtgccttccactaattttaaatttttcaacggacctacaaacaatgactctaccagcaccaata
            sacBay  tcaatagtgccttccgctaattttggttttttcaatgggcctactaataatgatactgcaaatctcaatg
            sacCas  acaacgataccttcgtccaatttc----------------------------------------cgaatg

     S. cerevisiae  gtactgttaacaacagcaatttcttttta------------aatagcaatgaaacgaaaccttcgaaaaa
            sacPar  ctaatgttagcagcaacaatttcttttta------------aatggcaatggtacaaattcttcgaaaaa
            sacMik  ctgatgctagaaacagtaatttctttcta------------aataaaaatggtgcaaactcctcgagaaa
            sacKud  ctgataataacaaagataatttctttttg------------aagggtagtggtgccggctcttcgaaaaa
            sacBay  ctaataccagcaacaacaatttctttttgaatggtaatagtaatggcaatggtgcggattcttcgaaaaa
            sacCas  caaggaacatc--caataatttcctatta------agtaataataacaacaataataattccacaaccaa

     S. cerevisiae  tgtcttcatggtgcactctacctctcaaaagaagtcgcagcaaccgttacaa--------aatctctcac
            sacPar  tgttttcatggtacactctgctcctcaggtcaagtcaggacagccgtcacaa--------gatctctcac
            sacMik  tgtgttaaaggtacactctgctgctcaagccaaacctcagcaacagtcacaa--------gatccctcac
            sacKud  cgtgttcatgctcaattccgctccccaggtaaaattgcagcaaccgttacaa--------gatttcccac
            sacBay  cgtcttcatgctaaattccgcttctcgacctaaatcgcaagaacagttgcag--------gaccacatac
            sacCas  tcccttaatacaaaac----ccgtttaggtcaaatt-tgtctacggttacgactattggtaactctccac

     S. cerevisiae  ------------actcaccttcctacacggaaaataaaccagataaaaagaaaaaatatatgataaacga
            sacPar  ------------actcaccttcccatatggaaaataaaccagatcaaaagaagaaatatatgacaagcga
            sacMik  ------------actcatcctcccatacaggaacaaaagccgaccaaaagaagaagtatatggtagcaga
            sacKud  ------------gctcagcatcacatgcgaggaaaaagccagaccagaagaaaagctttatgatcaatga
            sacBay  ------------cttcaccatcgcatacggagaaagcagctgaccaaaaaaaaaaatatatgatcaatga
            sacCas  aactgacaaccaatttgacttcagctgcggacaacacaatgttaaagcatagaaatttcataatgaatca

     S. cerevisiae  tgccaagaccattcaacttgtaggtccactgatttcctctcctgataat
            sacPar  tgataagacaattcagcttgtaggccctttagtttcctcccctgatagt
            sacMik  tgaaaaaactattcagattgtcggccccttagttgcctctcctgatatt
            sacKud  cgagaagaccatacagctgataggtcccttgattcaatctcctgacggt
            sacBay  ccaaaataccatacagcttataggtcctttgattgcttctcctgagagt
            sacCas  agacaacccaaaagag------agcctccttcttccaaatcctcaatcg

Alignment block 20 of 31 in window, 772192 - 773523, 1332 bps 
B D  S. cerevisiae  ttgggatttcagaagagatcgcacaaagcaagagaattaccaaggtttttgattaatcaagaacctcaat
            sacPar  ttaggattccagagaagatcacacaaaccaagagaattaccgaggtttttgattaatcaagaacctcaat
            sacMik  ttggggttccaaaagaggtcacataaaccaagagagttaccaaggtttttgattaatcaggaccctcaat
            sacKud  tttggtttccaaaagagaccacacaaaccaagagaattaccaagatttttgatcaatcaggaacctcaat
            sacBay  cttggtttccagaaaaaaccacacaatcccagagaattaccaagatttttaatcaaccaggaacctcaat
            sacCas  tttggatttaagcaattgaaacatactcccagggaaatgcccagatttttaattagtcaacaaccgcaac
            sacKlu  ttgggattccagcattactcacacccttttcgggatttacctcgttatttgatccaacaaataccccagt

     S. cerevisiae  tggagaaaagagcttttgtacaagatccctgggacaaggctaatcaagaaaaaatgatctcattggaaga
            sacPar  tgaaaaagaggatatttgtacaagatccctgggataaggctaatcaagaaaaaatgatctcattggaaga
            sacMik  tagagaagagagaatttgtacaagattcatgggataaaatcaatcaacagaaaatgttatcactagaaga
            sacKud  tggagcaaagaaaattcgtacaagatccatgggataaggccaaccaaaaaaaactactatccttggaaga
            sacBay  taaaacagagaaaattcgtacaagatccctgggataaggccaatcagcaaaagatgttaaccttggaaga
            sacCas  tggaacaaaagccctacaagcaagatccatgggataaagctaatcaggccaagatgtctaatttggaaaa
            sacKlu  tgaagccgaaacagttcatacaagatccatgggacaagtctaaccaggagaagatgctattactagaaga

     S. cerevisiae  atccatagatgatctcaatgaattgtatgaaacactaaaaaaaatgagaaatactgagcgatcgataatg
            sacPar  atccatagacgatctcaatgaattgtatgaaacgctgaaaaaaatgagaaataccgagcgtacgataatg
            sacMik  atccatagatgatctcaatgaattatatgaaacgttgaaaaaaatgagaaatacagagcgttcgataatg
            sacKud  atccatcgatgatcctaatgaattatacgaaacattgaagaaaatgagaaatacagagcgttcaataatg
            sacBay  atccattgacgatctgaacgaattatatgagacgttgaagcaaatgagaaataaagagcgttcaataatg
            sacCas  ttcaattgatgatctcacagaattatatgaaactctcaaaaagatgcgtgattctgaaagaaaaattatg
            sacKlu  ctccgtgagtgacgtggacgagctttacgaaacactaaaaaaaatgagagacgttgaaagaaaggtgatg

     S. cerevisiae  gaagaaaaagggctagtggataaagctgactcagcaaaggatctttacgatgctatagtatttcaaggta
            sacPar  gaagaaaaagggttagtagataaagctgattcagcaaaggatctttacgatgcaatagtatttcaaggca
            sacMik  gaagaaaagggattagtagataaagctgactcggcaaaagacctttacgatgcaattgttttccagggta
            sacKud  gaggagaaaggacttgtagataaagctgactcggcaaaggatctctatgatgcaatagtttttcagggca
            sacBay  gaggacaagggtcttgtagacaaggctgattcagcaaaggatctttacgatgcaatcgtatttcaaggta
            sacCas  gagaagaaaggtttagtagataaagctgattttgctaaggatttaaacgatgcaattgttttccaaggaa
            sacKlu  gaagatagaggacttgttgataaagcagatttagccaaggatttaaacgatgcaattgtatttcaaggta

     S. cerevisiae  cctgtttagatatgtgtcccacattcgagagatcaagaagaaacgttgaatatactgtgtattcatatga
            sacPar  cctgtctagatatgtgtcccatttttgaaagatcaagaagaaatgttgaatacacggtctattcatatga
            sacMik  cttgtctggatatgtgtcccatttttgaaagatcaagaagaaatgtggaatatacggtctattcatatga
            sacKud  cctgcctagatatgtgtcccatctttgaaagatcaaggagaaatgtggagtataccgtcttttcgtatga
            sacBay  catgtcaggatatgtgccccatttttgaaagatcaaggagaaacgtggaatacacagtcttttcatatga
            sacCas  cctgtgaagatatgtgccctatttttgaaagagcaagaaggaatgtcgaacacacagtcttctcttacga
            sacKlu  catgcttagatatgtgccctattttcgaaagggccagaagaagtgtggaaaacaatgtggtccgctacga

     S. cerevisiae  aaaaaatcaaccaaatgataaaaaagcttctcgaactaaagcattgaaggtcttcgcgaggccagcagca
            sacPar  aaaaaatcaaccaaatgacaaaaaggcttccagaaccagagcattaaaagtcttcgcgaggccagcagca
            sacMik  gaaaaatcaaccaaatgataaaaaggcttccagaaccaaagcactaaaggtctttgcaagaccagcggcc
            sacKud  aaaaaatcagccaagtgataaaaaggcctccaggtgtagagcactaaaagtcttcgcaagacctgcggct
            sacBay  aaaaaatgggccagacgataaaaaggcttccaggactagagcattaaaggttttcgcaaggcctgcagca
            sacCas  gaaggattctccaagttcaaaaaaggcatccgcaacaagagcgttaaaagtgtttgcaagacctgctgca
            sacKlu  aaaagatggttcagtaaattcacgtatttctaggtttcgtgctttaaaggtttttgctagacctgcagca

     S. cerevisiae  gcagcagctcctcctttaccttctgatgttaggccaccccatattttagtcaagacattggactatattg
            sacPar  gcagcagctcctcccttaccctctgacgttagaccaccgcatattttagtcaagacattggactatattg
            sacMik  gctgctgctcctccattaccatctgatgttaggccaccgcacattttagtcaaaacactggattatattg
            sacKud  gcggccgctcctcctctaccatcagatgtgagaccacctcatattttagtcaaaacattggactatattg
            sacBay  gcagcagccccccctttgccctcagacgttaggccaccacacattctagtcaaaacactggactatatcg
            sacCas  gctgctgcaccaccactaccctcggatgttagaccacctcatatattagtaaaatcattggattacatta
            sacKlu  gcagcagctcccccgttaccatctgatgttaggcctcctcacattctagtcaaaactttagactacattg

     S. cerevisiae  tagataacttattaacaacattgcctgaaagcgaaggattcttatgggataggatgagatccataaggca
            sacPar  ttgataacttattaacgacattacctgaaagtgaagggttcttatgggataggatgagatccataaggca
            sacMik  ttgataatttattgacaactttaccggagagtgaaggatttttgtgggacaggatgagatcgataaggca
            sacKud  ttgataatctattaaccacgttacctgaaagtgagggattcttatgggacaggatgagatctataaggca
            sacBay  ttgatcacttattagcaacattaccagagagtgagggtttcttatgggatagaatgagatctataagaca
            sacCas  tcgataaccttttaaccacacttccagaaagtgaaggttttatatgggatagaatgagatcaatacgaca
            sacKlu  ttgaaaatattgttccactattacctgattgtgaaagttttctttgggatagaatgagatccatcaggca

     S. cerevisiae  ggactttacatatcagaactattcaggacctgaggcggtagattgtaatgagcgtattgttaggatacat
            sacPar  agactttacgtatcagaactattcagggcctgaagcgattgattgtaatgagcgtattgtgaggatacac
            sacMik  agactttacttaccaaaattattcaggacctgaggcagttgattgtaatgagcgtattgtgagaatacat
            sacKud  agactttacgtatcaaaactattcagggcctgaggcagtagattgtaacgaacgtattgtaaggatacat
            sacBay  agattttacataccagaattattctggacctgaagcagtagactgtaatgaacgtattgtaagaatacat
            sacCas  agatttcacgtaccaaaattattgcggtcctgaagccatcgattgtaatgagcgaattgtaaggattcat
            sacKlu  agacttcacgtatcaaaactatagtggcccagaagcagttgattgtaacgagcgcatagtaagaattcat

     S. cerevisiae  cttctgatcttgcatatcatggtgaaatcaaatgtggaattttcgcttcaacaagaactagaacaattgc
            sacPar  cttctaattctgcatataatggtaaaatcaaatgtggaattttcgcttcaacaggaacttgaacaattac
            sacMik  cttttaattctccatgtaatggtaaaatcaaatgtggagttttcgcttcaacaagaattagaacagttgc
            sacKud  cttttgatactgcatgtcatggtaaaatcaagcgtggacttttcgctccaacaagagctagaacaactgc
            sacBay  cttttgatattacatgtcatggtaaagtcaaacgttgaattttcacttcaacaagaattagaacaattgc
            sacCas  ttgttaattttacatgtcatggccaaatctacagtcaaattttcattacaacaagaattagaacaattac
            sacKlu  ctcctaatattacacgtgatggccaaatttaacatcgagtatagcagtcaacaagagctggagcagttac

     S. cerevisiae  ataaatcccttatcacgttatcagaaatatacgatgacgttcgttccagcggcggaacatgcccaaatga
            sacPar  ataagtccctgattacattatcggagatatacgacgacgttcgttctagcggcggaacatgcccaaatga
            sacMik  ataagtccctgatcacattgtcagaaatatatgacgatgttcgctcttgcggtggaacatgtccaaatga
            sacKud  ataaatctttgatcacactatcggaaatatacgacgatgtccgttccagtggtggagcttgtccaaatga
            sacBay  ataaatctttgatcacattgtcagaaatatacgatgacgttcgttctagtggtggatcttgcccaaatga
            sacCas  acaagtcactaattactctatcggaaatttatgacgacgtacgagcaaatggaggtacctgtcccaatga
            sacKlu  ataaagccctgatcactctttctgaaatctatggtgaagtaagaagtcagggtggttcctctccaaatga

     S. cerevisiae  ggctgaatttcgagcttatgctctcctgagtaaaataagagaccctcaatacgatgagaatatccaaaga
            sacPar  agctgaatttcgagcttatgccctcttgagtaaaataagagacccccaatacgatgagaatattcaaaga
            sacMik  agctgaatttagagcctatgcccttctgagtaaaataagagattctcagtacgatgaaaatattcaaaga
            sacKud  agcagaatttcgagcttatgcccttttgagcaagataagggaccctcagtacgatgaaaatattcaaaga
            sacBay  agctgaatttagagcttatgctcttttgagtaagataagagacccccaatacgatgaaaatattcaaaga
            sacCas  agcggaatttagagcatattccttgttaagcaaaattagagatccgcaatatgataagactatacaggac
            sacKlu  agcagaatttagagcatactctttattaagtaaaataagagatccggagtatgacaaaatggcgcaagaa

     S. cerevisiae  ttaccaaaacatattttccaagataaattagttcaaatggctttatgtttcagaagggttatatctaact
            sacPar  ttgcctgaacatattttccaagataaattggttcaaacggctttgtgctttagaaggattatatctaact
            sacMik  ttgccacagcatatatttcaagataaattagttcaaacggctctgtgctttagaaggattatctcaaatt
            sacKud  ttaccaaagcatatcttccaggataaattggttcaaacggctttatgcttcagaagaattatttccaact
            sacBay  ttaccagaacatattttccaagataagatggttcagacagctttgtgctttagaagaatcatttctaatt
            sacCas  ttaccgacatatatagttcaagatgaactcgttcaactagccatttgttttagacgaatactttctaact
            sacKlu  ttaccggaacacgtatttcaagacgatctggtccagctagcattgtgctttagaaggatcgtatccaatt

     S. cerevisiae  cagcttatactgaacgcgggtttgttaaaactgagaattgtttaaacttctacgcaaggtttttccagtt
            sacPar  cagcttatactgaacgtgggtttgttaaaaccgagaattgtttaaacttttatgcaagatttttccaatt
            sacMik  cagcatatactgaacgtgggattgttaaaactgaaaattgtttaaacttttattcaagatttttccagtt
            sacKud  ccgcttatactgagcgcgggtttgttagaactgaaaactgcctaaatttctatgcaagattttttcagtt
            sacBay  ccgcatatagtgagcgtgggtttgttaaaactgaaaattgtctaaatctttatgcacgattcttccaatt
            sacCas  caaattatttggagagaggttatattaaaacggagaatggtataaatctttacactcgattttttacttt
            sacKlu  ctgcttacgtcgagagaggtcacatcaaaaccgaaagctgtatgaatttgtatgcaaggtttttcgaatt

     S. cerevisiae  aatgcaatcacccagcttgccattattaatgggattttttttgcaaatgcatctgacggatattagattt
            sacPar  aatgcaatcacccaacttgccattattgatgggattttttttgcaaatgcatctgacagaaattaggttt
            sacMik  aatgcagtcacccaatttgccattattgatgggattttttttgcaaatgcatcttacagaggtcagattt
            sacKud  gatgcaatcacccaatttaccgttattaatgggattttttctgcaaatgcatctgacggaaataaggttt
            sacBay  gatgcaatcacccaatttgccattgttaatgggatttttcttgcaaatgcatctaacagagatcagattt
            sacCas  aatgagatcagaaagggtaccatttttgatgaattcatttttacaaatgtatttaggtgaagtacgattt
            sacKlu  aatcaagtcagacaaagttccatttttgatgagttcattcttagaagtttatttgacagaaatccgcttc

     S. cerevisiae  tatgcgctaagagctttatcacatactttaaacaaaaagcataaaccaataccatttatttatttggaaa
            sacPar  tatgcgttgagagctttatcacatactttgaacaaaaagcataaaccgatcccattccactatttagaga
            sacMik  tatgcgctaagagctctatcacacactctaaacaaacgacataaaccaattccttttgtctatttggaaa
            sacKud  tatgctgtgagagccttatcacatactttgaacaaacggcataaaccgatcccactcgtctatttggaaa
            sacBay  tatgccgtaagagctttatcacatacattgaacaaacgtcataaaccaataccattggtctacctggaaa
            sacCas  tatgccatgaaagctctgagcttaacattgaacaaacgacacaaacctataccattagattatttacaaa
            sacKlu  tacgcatttaaagctttaagtttttctcttaacaagaagcataaatccgttccatttgattatttcatgg

     S. cerevisiae  atatgttactctttaataatcggcaagaaataattgaattttgtaattattactcaattgaaatcata-a
            sacPar  atatgctgctcttcaatcatcggcaagagataattgaattctgtgattattactcaattgaaatcata-a
            sacMik  atatgttactctttaatagttggcaagaaataactgaattctgcaattattattcaattgaaatcata-a
            sacKud  atacgctgttttttaatagtcggcaagaaataactgaattttgtaattactactcgattgaaatcatt-a
            sacBay  gtacactgcttttgaacagtcgacaagaaacaattgaattttgtaattactactcaatagaaatcatt-a
            sacCas  acatcttaatgtttaacgataaagaagaattgttagaattttgttcatattactcaattacggtggtaga
            sacKlu  atagttttcttttcaatgattatgatgagctcattgcattttgcaaatactattctattaaaatc----g

     S. cerevisiae  atggggatgcagccgatttgaaaacattgcaacattattctcacaaattgtcagaaacacaacctttaaa
            sacPar  atggagattcagcagacttgaaaacactacaacattattctcacaaattacccgaaacgcagccattaaa
            sacMik  acggagattctgcggatttaaaaacactacaaaattattctcataaattagcagaaacgcaacctttaaa
            sacKud  atggagattcagcagacttgaagacactgcatcattattcgcacaaattagcagaaactcaacccttgaa
            sacBay  acggagattccgctgacttgaagaccctaactcagtactcacataaactagcagaaacccaacctttgaa
            sacCas  atctggactc----gaattgagatcacttactcatcattcgcataaattacgagaaaagaagcccttgaa
            sacKlu  aaggtaatactgttgctttaaaaagtttaactcaccactcgcacatcttacccgatactaaaccgcctaa

     S. cerevisiae  aaaaacataccttacctgcttagaaaggagattacagaaaactacttacaagggtttaattaatggcggt
            sacPar  gaaaacatacttaaactgcctagaaagcagattgcaaaaaactacttatgagagtttaattaattgcggt
            sacMik  aaaaacttacttaaactgcttagaaagcagattacaaaaaactacctataagagtttgatcaattatggg
            sacKud  aaaaacgtatctaaactgcttagaggatagattgcagaaagttacttataagagtttaatagattgtggt
            sacBay  gaaaacgtacttaaactgtttagagaacagattacaagaaaccagttataagagtttgatcaattgtggc
            sacCas  gcaaagctatttgaaatgtgttgatgaaaaattaaggagaaaatcttataagcagctcataaactctgga
            sacKlu  gcaaagttatctgaaatgcgtcgatgaaaaattgcaacgcgttccattaaatcaactgattaatgcaggt

     S. cerevisiae  gaa
            sacPar  aaa
            sacMik  aaa
            sacKud  aag
            sacBay  aaa
            sacCas  aaa
            sacKlu  aaa

Alignment block 21 of 31 in window, 773524 - 773535, 12 bps 
B D  S. cerevisiae  gataatttggct
            sacPar  gatcattttgat
            sacMik  gaccattttggc
            sacKud  gaaaattttgat
            sacBay  aagaattttgat
            sacCas  cctaacattagt

Alignment block 22 of 31 in window, 773536 - 773957, 422 bps 
B D  S. cerevisiae  tcttcggtttatgtgaa---ggacccaaaaaaggatagaataccttctatcgcagaccaatcttttttaa
            sacPar  tctttgattaatgtaaaatcggctccggaaactgacagggtaccttctacagtgggtcaactttcttata
            sacMik  tcattttttgatataaaatcaaatttgaagaatgatataaattctccttccgttacccaatcctgtctaa
            sacKud  tcattagctggtgtaaaatcggataggggaaatgataaaatctcaattactgctgaccaagttttttcaa
            sacBay  tcactgactggtgtaaaattgtattcaagaagtgttgaaacgtcctctgccattgaacagcccttctttc

     S. cerevisiae  tggaaaattttcaaaacaactataacgaaaagttgaaccaaaactcatccgttaaaccacagatcaatac
            sacPar  tgaaagattctgctaacaactttaacgtaaagttgaaccaaaaccaatcttttgaaccgaagattaatga
            sacMik  tgggtgacgttctcaatgaccataacgtag---tgaaccgaaactcatcttttgaaccgcaaattaatat
            sacKud  aaaaaaatatccccagcaatcccaatatgatgtttagccaacacactccctttgagtctcaaatcgtcgc
            sacBay  aaacacatacggagaataactccaacatgttacttaaccaaagtactctttttgaaccaaaaattcttgc

     S. cerevisiae  ctctccaaaaagagttgcaacgcggcccaaccactttcctttttcacaggaatctaagcaattacctcaa
            sacPar  ctccccaaacagaactgtcacgcggcccaatgactttcctttttcacagcaatttaagcaatcctcccaa
            sacMik  cccttcaaaggcaatcaacattcaacccaaggatcatcttttatcgaaacaatctaaacaatctactgaa
            sacKud  tccttcaagattgaacaaaattgaacccagggagatcttatttccactacaatctaaacaatctgctcaa
            sacBay  tccttcaaaaataggcactaccaaacccatggtaactttttctccacaacaaattaaccaaccatctcga

     S. cerevisiae  atatctcaatctcatactttatcaacaaatcctttattaacgccacaggttcatggagacctatcggaac
            sacPar  ttatctcaatctcaaactttaccaataaatcctttattaactcagcaggttccgggatacttatccggac
            sacMik  atatctcaatttaagagttcatcaacaaatgcttcatcgactcagcaggtaccggcatatctatccgaac
            sacKud  ccatctcaatttcaaacgtcattcaacaagcctccgttaacccagacggctcctacatatctacctgagt
            sacBay  gcatctcaatttcaacctttaaaaaacaatgctccattgcctcagacgacacctgcatttcaattcgagc

     S. cerevisiae  agaaacaacaacaaattaaaaccgtcacagacggaggctccccatttgtttttgatcaatcagcacagaa
            sacPar  agaaacatgaacaaattaaaactgttacaggtgcaagttccccacctctttttaatggatcattaccaaa
            sacMik  agaaacat---cagtttaaaaacattgtagatggagggttcaaatctttttctaacggatcattaccgaa
            sacKud  ggaagcaacaaaaaatcaaaagcgtaacagccggaagtcctcaatctttaattagtcaattgtcacaggg
            sacBay  agaaacaacaacaaactaaaagtgcggcagatagaaactttcaaccagttattagccaattgccaccgag

     S. cerevisiae  ttctacggtggaagcttccaaggctcatatgatatccacgaccagcaatggtgcatatgatgagaagt--
            sacPar  ttctgaagcgcaagcttccaaaatttatatgaaatccacaaccaacaatggtatgtatgacgaaaaattc
            sacMik  tcctgaagtgcaagcatccgaacctttcaggataccaactaccattaatgatacagatgacgaaaaatca
            sacKud  tcctgaagggcaagtctttggaaatggaaggatgaccactacagacaatggtacagataatgcaaagtgg
            sacBay  ctctgaagcgcaagttacaggacctgaaaagatattaactactgatattgatacggatgattcaaagctg

     S. cerevisiae  -------tgagttc
            sacPar  gagagtgtgagtac
            sacMik  aagagtaagagtct
            sacKud  aagagtgaggagct
            sacBay  catcgtgagaggct

Alignment block 23 of 31 in window, 773958 - 773964, 7 bps 
B D  S. cerevisiae  agagcaa
            sacPar  agagcaa
            sacMik  agaacaa
            sacKud  ggaacga
            sacBay  tagagaa
            sacCas  acaggat

Alignment block 24 of 31 in window, 773965 - 774612, 648 bps 
B D  S. cerevisiae  gaagaaatgaggaaaaaagaagaacaacgaatagaa-------------gaagagaaaactcaattgaaa
            sacPar  gaagaaatgagaaggaaagagatagaacgaatggaa-------------gaggagaaagcacaattgaaa
            sacMik  gacgataagtggagcaaagaaaaacaaagaattgaa-------------aaagaggaaattttgttgaaa
            sacKud  gatgaaaacgaaagaaaggaactggaaaaaaggaag-------------gaggagaaagttcgcttgaaa
            sacBay  gaagaaatgaaaaataaagaactagaaagaatgaaa-------------gcagaaaaaattcgcctg---
            sacCas  gaagaaaatcggaagtttgaagttgaaagattaaaa-------------gaacgaaag------------
            sacKlu  gaagaaa---aaaggaaagaattagaacaaaggagaatactacaagaacagagggataatgaaattcaaa

     S. cerevisiae  aagaagcaagaaaatgcggaca----------aacaagtaatcact------gaac-------------a
            sacPar  aaaaaaagggaagatgctgaca----------agcaaaaaatcacg------gaac-------------a
            sacMik  aagaaacaggaaaatgagaaca----------aaaaggaaatcaca------gaac-------------a
            sacKud  aaaaaagaggaagatgcaaaca----------agcaaataatcacc------cacc-------------a
            sacBay  aagaaacaggaggatacgaaca----------aagaaaatgtcgtt------cacc-------------a
            sacCas  aaaaaaattgaagatgagg----------------------cttct------aaac-------------a
            sacKlu  aacaaaaattggagcaagaacaacatgccaagagggaaaaattacttcagcggaacactatactttctga

     S. cerevisiae  aattgcaaatgacttagtaaaagaagtggtaaacagtagcgtgattagtattgttaagcgtgaattttca
            sacPar  aatagcaaatgagttgatagtggaaatagtaagcagtaacgtgactgatattgttaagcatgaagtctca
            sacMik  aatagcaaatgagttaataaggaaagtagtaaacagcaacgtcactaatattgttaaggatgaaatctca
            sacKud  aattgcagatgagttaataaaggatgtagtaaacggtaaagtaactgaaattgttgagtatgagctctca
            sacBay  aattaccaatgaactgataagagaagtagttaaaaacaaagcaagtgaaattgttaaaggtgagctttca
            sacCas  aatactgggcactttactaaaagatgttgttcatcgattagctgttgacactgttaaatcagcaatgaat
            sacKlu  actaacagatgaattaaccagaaaggttgtgcaaaaatttgtccacaatatagtcgaagaccgtataaaa

     S. cerevisiae  gaggctaattataggaaggacttcattgacacaatgacccgtgaactttatgacgcatttcttcatgaaa
            sacPar  gaggttaaatataggaaaattctcgttgacaagatggcccatgaactttatgatgcatttcttcatgaaa
            sacMik  gaggttaatcatagaaagaagcttattgaaacgatgactcaggaactgtataatgcctttgtacatgaaa
            sacKud  gagatgaacgagaggaaaaccatcatcaagacgatgacgcgtgaactttatgatgcattcattcacgaaa
            sacBay  gaagttaatcgcaggaaaaagctcattgatacaatgtcttatgaactttatgatgcatttattcatgaaa
            sacCas  gaggcaaacgaaaaaaaagtacttgtcgaagatatttcggaggaattgtatcaagcattcattcatgaaa
            sacKlu  agaaagaatgaaaaggaaacaaagctggaaatactttctgaagaactttatcatgccttcattcacgaaa

     S. cerevisiae  ggttgtatctgatatatatggattctcgtgctgaa-------------ttgaaaaggaattccactctaa
            sacPar  agttgtatctgatatacatggattctcgcgctgac-------------ttgaaaaggaatactgctttga
            sacMik  aactgtatctcatatacatgaatactcgtgctgaa-------------ttgaaaaggaactttaccttaa
            sacKud  aactatatctcatatacatggattcttgtgctgaa-------------ctgcatagacattattccctga
            sacBay  gattatatcttatatatatggattcacgcgctgaa-------------ttaaaaggaaattatgcgctaa
            sacCas  aattgtatttaatttatcttgaaacaaaagctgaagcatgtgatatgcgtggaaagaaa-------ctta
            sacKlu  agctatatttcacatttttggaaactaaggcggaaaaa----------tttgaagaaagcaggacttt--

     S. cerevisiae  aaaaaaagttctttgagaaatggcaagcatcttattc--tcaagcaaagaagaaccgtatattagaagaa
            sacPar  gaaagcggttctttgagaaatggcaagcatcttattc--tcgagcaaaggagaatcgcatactagaagag
            sacMik  gaaaacagttcttcaagaagtggaaagcatcttatta--tctggccaggaagaacagagcaatagaagaa
            sacKud  tgagacgattcttaaagaactggaaatcattctatta--caaagccaaaaagaatcgcgcacttgaagag
            sacBay  tgaaacgtttctttaacaaatggcaaatatcttattc--taaggtcaaaaagaatcgcgcattcgaagag
            sacCas  gaa------tttttcatatctggaaaaaaaaatatgaagccaaggtaaaggaaaaggagctattgaagaa
            sacKlu  -gaaacgaggcctagataaatggaaaggaaagtataa--ctctcgcaaattgaatcgtgaattgaagaag

     S. cerevisiae  aaaaaaagggaagaaattaaact------cgtaagccaccaattaggagtacc-----aggttttaaaaa
            sacPar  aagaagagggaagaaatcaagct------cgtgagccaccaattaggagtacc-----gggccttaaaaa
            sacMik  aaaaagaggaaagaaatcgaact------tgtcagccatcaactaggagtacc-----gggcttcaaaaa
            sacKud  cgaaagagggaagaaattgaact------cgtgagccatcaattaggagtacc-----tagcttcaaaaa
            sacBay  caaaagaaggaagaaatcgaact------tgtgagtcaccaattaggagtacc-----aagcttcaagaa
            sacCas  aagaa--------aattcgaactgactaaaataagcaaacaacttggtgtacc-----ttctttcaaaaa
            sacKlu  agaaaaagagaggaacttttgca------agtgggcaaaatgttgggtgttccgacttgggctcccaaaa

     S. cerevisiae  atc-----tacatgtttgtttagaa------------ccccgtataagggtaacgtaaattcatcattta
            sacPar  atc-----tacctgcttactgagaa------------ccccgtataagggtaatgtgaattcatcattca
            sacMik  aca-----tacctgttcattgaaaa------------ccccatataaaagtaacgcgaattcatcattta
            sacKud  atc-----cacccgcttattgagaa------------ccccatataagagtaacgtggattcgtcattta
            sacBay  gcc-----tacttgtttattgaaaa------------ccccatacaagagtaatgtagattcgtcattta
            sacCas  atcaagaatacat-----ttaggaa------------ctccgacaaatgggaac---aactcttctttct
            sacKlu  aga-----aa--------ttgagagcattgaatgactctccagatattggtggtaataattcctttttat

     S. cerevisiae  tgctatcatcttcagataag---aatctaatattttcacctgtaaatgacgagtttaataagtttgcgac
            sacPar  tactatcgtcttcagataag---aatatagtgttttcacctgtaaatgacgagttcaacaagtttgcaac
            sacMik  tactatcatcttcggataaa---aatataatattatcgcccgtaaatgatgaaatcaacaagtttgcaac
            sacKud  tgctttcatcttcagataagaaaaatataatcttttcacctgtaaatgatgaattcaacaaattctcaac
            sacBay  gattatcatcttcagataagaagaatataactttttcacccgtcaatgatgaattcaacaaatttgccac
            sacCas  tacaactatctgatagaaagaataattttacattttctcctataccaaatgaaactaataaatttccttg
            sacKlu  taccctcatcttcggccagc---caaatactttatactcctacaccaaatgaagacaatcatttcactac

     S. cerevisiae  tcatttaaccaagatttcaaaactttggcggccgttagagatgcaatcaatctattatga------taac
            sacPar  tcatttaacaagggtctcgaaactatggcaaccgttggatatgcactcaatctattatga------taac
            sacMik  acatttgacgcaaacctcgaaactgtgggaaccattggatatgatatcaatttattatga------taag
            sacKud  tcattcaaccagaacctcgaaactatgggaaccattggatatgaaattgttttattatga------taag
            sacBay  ttttttaacgaaaacctcgaaattatgggaacctttggatttgaaatcaatttattatga------taaa
            sacCas  cggggagaagaaaattggcgatttgtgggaaaaagttgatattgagtcaatatatctgaaggtagttaac
            sacKlu  acctgtgcgtaagaactcgactatatgggaaccaatcaatctaaaagaaatatatttcaa------taga

     S. cerevisiae  ctt-----------------------acgaaaaag-ttccc----------------ctccaattctctt
            sacPar  cta-----------------------acggagaag-tttcc----------------cgctaactctctc
            sacMik  ctt-----------------------acggaaaaa-tttcc----------------tgcgaacgttctt
            sacKud  atg-----------------------actgaaaag-tttcc----------------tgtcgactttctt
            sacBay  ttg-----------------------actaagaaattttcc----------------cgcaaatcttctc
            sacCas  gac-----------------------ttgaagaaa-tatga----------------agaaaa------t
            sacKlu  attgctttgaatttggaatcgattgaacggagaga-tctaaaaaggtcatctaatagtatggattttagg

     S. cerevisiae  actccggcaaat
            sacPar  actccagcaaat
            sacMik  acaccagcaaat
            sacKud  accccagcaaat
            sacBay  actcctgctaat
            sacCas  atacaattgaat
            sacKlu  cctccaaaaaat

Alignment block 25 of 31 in window, 774613 - 774915, 303 bps 
B D  S. cerevisiae  ------ttattcatttatgcgaaagattggacatcactctcaaaccgttggatcttaagtaagttc-aat
            sacPar  ------atattcatttatgcgaaagattggacatcgctctcaaaccgttggatcttgagtaagttc-aac
            sacMik  ------attttcatttatgccaaagattggacatctctttcaaatcgttggatcttgagtaaattt-gac
            sacKud  ------atttttatttatgctaaagattggacatcgctctccaatcgttggatcttaaataaattt-aac
            sacCas  ------attctcctttactctaagagctggattttccttccaaataagtggatcctaaagaaatttggat
            sacKlu  gtcgacatatttatctattcaaaagattggaactccatatctggcagttggttattaagcaaattcgggc

     S. cerevisiae  ctgcaaacagcacagg--attcaaaaaagt-tcagcaataatattatctctagcagaataatttgtattg
            sacPar  ctgcgaactatgcagg--attcaaaaaatt-tcaacaataatattatttccagcagaataatctgtatta
            sacMik  ttacagactatgcagg--attcaaaaaaat-tcagtaatactgtcatttctagcagaataagctgtattg
            sacKud  ctacagtgcatgcagg--attcaaaacatt-tcaataataacgttatcaccagcgagataagatgtatcg
            sacCas  taataacccctacaag----tcatattaatatcaaaaaggaaaatgtatcaatcaacattagttgtttca
            sacKlu  tgggagacaacccaagacacccgaaaaata-ttactagcaaacaattatctgtgaattttacaccttgtg

     S. cerevisiae  atgatgaatatgaaccatctgattttagtgatttgcaattattgatatttaatacaggtgttacgaatcc
            sacPar  atgatgaatatgaaccatccgattttagtgatttgcaattattgatatttaatacaggtgttacgaatcc
            sacMik  atgacgattatgagccagctgattttagtgatttgcaattattgatctttaatacaggagttacaaatcc
            sacKud  atgataattatgaaccgtccgattttagtgatttgcaattattgatcttcaatacgggtgttacaaatcc
            sacCas  acgaaggagacaatatatcacaagcttcaaatttacagttactggtttttaacacaggtgttacagattc
            sacKlu  aaccggagtttgtcccttctgtctttcagaacctgcagcttctagtgttcaatactggtgttaccgattc

     S. cerevisiae  agatatattcgatttggagatgaaactgaaagatgatggtgaagaactgataaagctaatcactggtatt
            sacPar  cgatatatttgatttggagatgaaactaaaagatgacggtgaagaactcataaagttaattaccggtgtt
            sacMik  agatatatttgatttagagatgaaactaaaagatgacggcgaagagctgattaagctaattacaggtgtt
            sacKud  tgatatatttgatttagaaatgaaactaaaggatgacggcgaagagctgataaaattgattactggtgtt
            sacCas  aaatatatttgatcttgaaatgaaactcaaacaagatggagaggatctgatcagacttatcaataaggtt
            sacKlu  agatatatttgatttggagatgaagctaagacaagacggtgagaagttaatcgaactagttcatggtatt

     S. cerevisiae  tctctaaatacaaatatatgcttttcccttttg
            sacPar  tcgctaaatacaaacatgtgcttttcccttttg
            sacMik  tcgctaaatacaaatatatgcttttgccttcta
            sacKud  tccctgaataccaacatatgcttctgcctttta
            sacCas  tgccaagtttccaatattcgcttcagtattatt
            sacKlu  gttttgaataccaactacaagtttgaaattatg

Alignment block 26 of 31 in window, 774916 - 775450, 535 bps 
B D  S. cerevisiae  attatt-tattgggaatccgcagaaaatactttatcggaaagtaccattaaac---acttactaaaatta
            sacPar  attatt-tattgggaatccgcagaaaatacattatcggaaactaccatcaagc---aatcgctgaaatta
            sacMik  attatt-tattgggaatctgcagagaatacactatcagaaagcacaatcaaac---agtctctaaagctg
            sacKud  atcatt-tattgggaatctgcagaaaatacgttgtcggaaactactattaaac---agtctttaaagcta
            sacBay  attatt-tattgggaatctgcggaaaatacgctatcggaaggtactatcaaac---agtccttaaaacta
            sacCas  -ttgtcatattgggactcgaaagaa---acaccattagatgatgatgttatatccaaatttttgcagctt
            sacKlu  cttatt-tactgggaatctgcagaaagtccattaaaccttaatgagatttcaa---agtttctgaaacta

     S. cerevisiae  aaccgaatatctaaaaactatagtagtgttattgaacgcattgacttaatgaatctcaccgaagaatccc
            sacPar  aaccggatatctaaaaactatagtagtgttgttgaacgtatcgatttaatgaatctcactgaagaatccc
            sacMik  aacagaatatccaaaaactataatagtgtcatcgaacgtattgatttaatgaacctcacagaggaatacc
            sacKud  aatcgaatatctaaaaactatggcagcgttattgaacgtattgacttgatgaatctcacggaggaattcc
            sacBay  aatcgcatatctaaaaactataacagtgttattgagcgtattgacctgatgaatctcacggaagaatccc
            sacCas  aatcgcattcataaaaatttcaaaaacatcatagagcaaattgttattatacatattaatgatcaatctc
            sacKlu  aacaagatttcaaaaatttttggtaccgttataacaaagatagaaattgtcaagatgaccgatgataacc

     S. cerevisiae  ctcataagtgtttagaggacaaattatctgaaatatcacattcttatgtgtacaagttgacagaaagggg
            sacPar  ctcataagtgtttggaagacaaattatacgaaatatcacaatcttatgtgtacaagttgacagaaagggg
            sacMik  ctcataagtgtttagaagataaattattcgaaatatcacaatcttatctattcaaattgactgaaagggg
            sacKud  ctcataaatgtttggaggataaattatccgaaatatcacagtcttatgtgtacaagctgacagaaagagg
            sacBay  ctcataaatgtctggaagataaactatctgaaatctctcattcatatatttacaacttgacggaaagagg
            sacCas  ctcataaaatcctagaaaaaggtttactctattgttcagaaaagtttaaatacagattaacggagagagg
            sacKlu  cccacaaaagattagcagatggattgacaaatatggcctctagcttcaaatttgaattaactgaacgtgg

     S. cerevisiae  aaaatatgataaaacacttcgtcagaaaaggtcattggccggca----------------ttcactcacg
            sacPar  aaaatatgataaaacgctccgtcagaaaagatcattggctggca----------------ttcactcacg
            sacMik  aaaatataacaaaacacttcgtcagaaaaggtcattagctggta----------------tacactcacg
            sacKud  gaactataataaaacacttagtcaaaaaagggccttggctggcg----------------tccactcacg
            sacBay  aagttacaataagacgcttcgtcaaaagaggtcattggctggaa----------------tccactcacg
            sacCas  aaagtacatccatgctttagaccaaagacgttctcttgctggta----------------ttcagaca--
            sacKlu  tgagtacaaccaatccttgcagcaacgaagcctgtcaggtgtcagcatagccgcatattcttcacctttc

     S. cerevisiae  tagcactcaactacaaactacaaaggacattgatcaaaagatgaagaaaatgctggaaaaagagaaaaac
            sacPar  taacactcaattacaaactacaaaggacattgatcccaagatgaacaaaatgctggaaaaagagaaaaac
            sacMik  tagcacccaattacagactacaaaggacattgacaaaaaaatgaaaaatatgttggaaaaagaaaagaaa
            sacKud  taatactcaattacagactacaaaggatattgatcaaaggatgaagaaaatgctggaaaaagaaaaaaac
            sacBay  taactcccaattacagactacaaaggatatagatgaaaagatgaagaaaatgctagaaaaggagaaaaac
            sacCas  ----aagtcgttacagaaatcaacatcagtggagcaaaaaatgaaagaaatgttagaattagaaaataaa
            sacKlu  caat-ctcacctaatgaacaccaaaaatatagacgagaaaatgcatagaatgctagaattggagaaggaa

     S. cerevisiae  aagtacca-acaacaaataggcgaaaggaacacctatgcgcatctagaatcgcatatagatgcatcacca
            sacPar  aaatacca-acaacgaatgggcgaaaggaacacctacgcgcacctagaatcgcatatagccgcatcacca
            sacMik  aagtacca-acatcaaattggtgaaaggaacacctacctgcatctagaatcacatatagccgcttcacca
            sacKud  aaatatca-acaacaaatgggtgaaaggaatacttacgcacatcttgaatctcatatagcagcatcacct
            sacBay  aaatacca-acagcaaatgggtgaaaggaatacttacgcacatttaggatctaatatatctgcttcacct
            sacCas  aaacaaaagatgacgaaaagac-aaagagagaagtattctaatttgcagtcacatattatagcgtccccc
            sacKlu  aagtacga-aaaagaaaaagacctaaaaaacacgtatgcccacctgagatctcacgccgaagcgtcccca

     S. cerevisiae  agaagcaagaaaaggaaattgccaatactcctgtcaacttctcattcgagccaa------tttaaaactc
            sacPar  agaatcaagaaaagaaagctgccaatacttctgtcaacttcccattcgagccaa------ttcacaaccc
            sacMik  aaagtcaagaaaagaaagctgccgatacttttatcatcttcccattcaagccaa------ttcaaaacgc
            sacKud  agggccaagaaaaggaagctgccaatactttcatcatcctctcattcaagtcaa------tttaaaacac
            sacBay  aggatcaaaaaaagaaagttgccaatacttttgtcgtcctcacactcaagtgaa------ttcaaaacac
            sacCas  aggaattgtaaaggaaagttaccaattct------actttctgaatcgagaagagcgagctttaagacgc
            sacKlu  agatctcgtaaaggcaaacttcaagttttgttatcagagtcgcttaagaataaa------ttcaagacgc

     S. cerevisiae  ctctagcttcta-------gattaaacacct-ctggt--tcgtctacttcaccacctttaccatctcatt
            sacPar  ctttagcttcta-------gaccaaatacatcctggt--acatctacctcaccaccactaccatctcatc
            sacMik  ccctagcatcta-------gactaaatacat-ctggt--acgtctacatcaccaccactaccatctcatt
            sacKud  ccctagcatcta-------ggctgaatacgt-ccggt--acatctacatcaccaccactaccatctcact
            sacBay  ccttggcgtcta-------gactaaatacgt-ctggt--acctcaacatcaccaccactgccatcccatt
            sacCas  ctgtcg-------------gaccaaatactt---------cattattctcaccaccagtcccctcacatc
            sacKlu  cactacccattattgtgtcagataaacggtt-atcgtcatcattttcattgccatcagtatcatctcacc

     S. cerevisiae  tggcgatgaaat
            sacPar  tggcaatgaaat
            sacMik  tggcaatgaaat
            sacKud  tggccatgaaat
            sacBay  tggcaatgaagt
            sacCas  tagcagtcaaag
            sacKlu  tagccgcaaaag

Alignment block 27 of 31 in window, 775451 - 775479, 29 bps 
B D  S. cerevisiae  tcaggaaa-----------------aactcgagagtta-ctagttta
            sacMik  tcaggaac-----------------aattcaagggtta-ctagttta
            sacKud  ttagaaga-----------------aactcaagagtta-ctagtctg
            sacBay  ttaggaaa-----------------aattcaagaatta-ctagtttg
            sacCas  ttcgcaaacgttttggcacatctttacctccggagaca-cctagtta
            sacKlu  ttaaaatg-----------------aatgcactaagtacccagccta

Alignment block 28 of 31 in window, 775480 - 775525, 46 bps 
B D  S. cerevisiae  cataccgtattacccgttagcacaccaagccatagtaataacatac
            sacPar  cataccgtattgcccgttagcacaccaagccatagtaacaatttgc
            sacMik  catactgttctccccgtcagcacaccaagtcacagtaacaatttgc
            sacKud  catactgtactgcccgttagcacgccaagtcatagtaccaatttgc
            sacBay  catactgtactgcccgttagcacaccaagtcacagcaccaatttac
            sacCas  cagtacaaactaccca--aattcagtaattcatcaaaaaact---c
            sacKlu  ----------cgctgcttggtactccaagccacacgagaaacacac

Alignment block 29 of 31 in window, 775526 - 775775, 250 bps 
B D  S. cerevisiae  cagctgcaagttttagcgggaataacaccaccgatatacagtcccaacaat-taatcgaaaaccagaaga
            sacPar  cagcctcaagttttagtgggaataacactacggatatacaatcccaacaat-taattaaaagtcagagga
            sacMik  cagctacaagtttaaatggcaatagcagcacagatatacaaccccaacaac-cgactgaaaatcaaagga
            sacKud  cggctactaattccaacggcaatgacgccacagatactcaatctcaacaat-tgacgagaagcaaaagaa
            sacBay  cagcttcaaattcggacgctacggcaactacagatatacattctcaacagt-caagtagaaatccaagaa
            sacCas  ctgtgacaagaattatcggaa------cctctgatgaacaa----aacaatgcaaccataggcca-----

     S. cerevisiae  gtacatctgtatatttgaataacgtttctgaaaggattctgggaaatcaagaaatatgtcaaacaccaat
            sacPar  gtacatgtgggtattcaaaaaatgtttttgaaagagtcccaggaaatcaggaaatatgtaaaacaccatt
            sacMik  gcatggccgagagttcgaataatgcttttgaaagagttccggaaagccaggacatatacaaaacaccaat
            sacKud  gtactctagaactttcaaatagtgcttttgatagagtacccgaaagccaagccatatatcaaacaccgac
            sacBay  gcacggtaggacttttaagcggtgcatttgaaagagcatcagaaagccacgcaatatgtcagacaccaat
            sacCas  -tatgcttcaatcttctcgtt---------------------------------tatttgaaactccaat

     S. cerevisiae  taatcctgttactcctgt--------gctagatggggccgatcaaggtaaggaggacattcctgatagta
            sacPar  taaccctgtaactccagt--------gttagatggagccgatcaaggtaaggaggacattcctgatagta
            sacMik  taacgctgtcactccagt--------gttagatagcaccgatcaagataagagggatattcctgatagta
            sacKud  taa---tgtcgctccagc--------gtcagataacactgatcaagataaagatgacattcctgatagca
            sacBay  aaacaatgcggcccctgc--------gtcggagggctgtgataaaaacatagatgatattcccgatagta
            sacCas  aaattctacagctgcctcccattctggccagaatacaccga-----------aggacattcccaacaacg

     S. cerevisiae  tattagagctgaagatcttgatcgattctgtcaagaagaaagtaaataa
            sacPar  tattagaactcaaggttctgattgattctgtaaagaaaaaagtaaataa
            sacMik  tattagaacttaagactctgatcaattctgtcagaaaaaaagtaagtaa
            sacKud  tattagagctcaaaattttgatcgattctgtcaagaaaaaagtaaataa
            sacBay  tattagaactcaagatcttgatcgattctgtgaagaaaaaagtaaataa
            sacCas  tacaagaattgaaatatttgattgaatctgtgaaaaagaaggtgaacga

Alignment block 30 of 31 in window, 775776 - 775918, 143 bps 
B D  S. cerevisiae  tgattaaa-gcatttgtaatatataaacaaaagaaatgtgcattttttctatagctttaggaaaagta--
            sacPar  tgattaaaagcatttgtaatatataaataaacgaaatgtacattttttctgtagggttatgaaaaaag--
            sacMik  cgattaga-acatttgtaatatataaa-aaagaaaatatgcatttttaatacaagatcatgaaaaaatgc
            sacKud  tgactaga-acgtttgtaatatataaataaaaaaaacatgcatttttaacgtagaatctcgaaaaata--
            sacBay  cgaataaa-atatttgtaacatataaatgataaaa---tgcatttaataaacgatctttgaaaaaatg--

     S. cerevisiae  -tttgataatcacccatttcttattactgtcggcgtgccat-ccttttctctaaagct---cggcggaag
            sacPar  -tacta-atttacgcatttcgtattactgctaatatttcat-tcttttctctaaagct---cggcggaag
            sacMik  cttaaa-atatacatatttcgtcttatagctattgtgcaataatttttcgctaaacttctgcggcggaag
            sacKud  -tctgt-tatccccaattccgcggtcgagctatc-tttgtt-ctcttttttgaaagtt---cggcgggag
            sacBay  -tctga-tagcacgtattttggcttgcttctat------at-tttgttcttta----t---gcgcagaat

     S. cerevisiae  -aaaaattcaat
            sacPar  gaaaaatccaat
            sacMik  -gaaaacccaat
            sacKud  -gagaatttaat
            sacBay  -gaaaattcaag

Alignment block 31 of 31 in window, 775919 - 775965, 47 bps 
B D  S. cerevisiae  aattgtgc-a-------aaaagtgaagccagggttgcaaatacagagttat-atca
            sacPar  ggttgtcc-a-------aaaagtgaagccaggacttcaaccacagagtttt-gtta
            sacMik  ggttgttcaa-------aaaagtgaagccaaaact-caagtacaaagtttcaagca
            sacKud  cattgtgt-aaaaaagtaaaagtgaacccaggctttaaagtacaaactttt--gca
            sacBay  aattgtgt-g-aaaagtaaaagcggagccaaacttcaaagtacaaactctt-atga
            sacCas  ------------aattttataataaaaccacagccgcgaacctagcgcaac-aata

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Go to Conservation track controls

Data last updated at UCSC: 2011-09-01

Description

This track shows a measure of evolutionary conservation in seven species of the genus Saccharomyces based on a phylogenetic hidden Markov model (phastCons). The graphic display shows the alignment projected onto S. cerevisiae.

The genomes were downloaded from:

Display Conventions and Configuration

In full and pack display modes, conservation scores are displayed as a wiggle track (histogram) in which the height reflects the size of the score. The conservation wiggles can be configured in a variety of ways to highlight different aspects of the displayed information. Click the Graph configuration help link for an explanation of the configuration options.

Pairwise alignments of each species to the S. cerevisiae genome are displayed below the conservation histogram as a grayscale density plot (in pack mode) or as a wiggle (in full mode) that indicates alignment quality. In dense display mode, conservation is shown in grayscale using darker values to indicate higher levels of overall conservation as scored by phastCons.

Checkboxes on the track configuration page allow selection of the species to include in the pairwise display. Configuration buttons are available to select all of the species (Set all), deselect all of the species (Clear all), or use the default settings (Set defaults). Note that excluding species from the pairwise display does not alter the the conservation score display.

To view detailed information about the alignments at a specific position, zoom the display in to 30,000 bases or fewer, then click on the alignment.

Gap Annotation

The Display chains between alignments configuration option enables display of gaps between alignment blocks in the pairwise alignments in a manner similar to the Chain track display. The following conventions are used:

  • Single line: No bases in the aligned species. Possibly due to a lineage-specific insertion between the aligned blocks in the S. cerevisiae genome or a lineage-specific deletion between the aligned blocks in the aligning species.
  • Double line: Aligning species has one or more unalignable bases in the gap region. Possibly due to excessive evolutionary distance between species or independent indels in the region between the aligned blocks in both species.
  • Pale yellow coloring: Aligning species has Ns in the gap region. Reflects uncertainty in the relationship between the DNA of both species, due to lack of sequence in relevant portions of the aligning species.

Downloads for data in this track are available:

Base Level

When zoomed-in to the base-level display, the track shows the base composition of each alignment. The numbers and symbols on the Gaps line indicate the lengths of gaps in the S. cerevisiae sequence at those alignment positions relative to the longest non-S. cerevisiae sequence. If there is sufficient space in the display, the size of the gap is shown. If the space is insufficient and the gap size is a multiple of 3, a "*" is displayed; other gap sizes are indicated by "+".

Codon translation is available in base-level display mode if the displayed region is identified as a coding segment. To display this annotation, select the species for translation from the pull-down menu in the Codon Translation configuration section at the top of the page. Then, select one of the following modes:

  • No codon translation: The gene annotation is not used; the bases are displayed without translation.
  • Use default species reading frames for translation: The annotations from the genome displayed in the Default species to establish reading frame pull-down menu are used to translate all the aligned species present in the alignment.
  • Use reading frames for species if available, otherwise no translation: Codon translation is performed only for those species where the region is annotated as protein coding.
  • Use reading frames for species if available, otherwise use default species: Codon translation is done on those species that are annotated as being protein coding over the aligned region using species-specific annotation; the remaining species are translated using the default species annotation.

Codon translation uses the following gene tracks as the basis for translation, depending on the species chosen (Table 2). Species listed in the row labeled "None" do not have species-specific reading frames for gene translation.

Gene TrackSpecies
SGD GenesS. cerevisae
No annotationall the other yeast strains
Table 2. Gene tracks used for codon translation.

Methods

Best-in-genome pairwise alignments were generated for each species using lastz, followed by chaining and netting. The pairwise alignments were then multiply aligned using multiz, and the resulting multiple alignments were assigned conservation scores by phastCons.

The phastCons program computes conservation scores based on a phylo-HMM, a type of probabilistic model that describes both the process of DNA substitution at each site in a genome and the way this process changes from one site to the next (Felsenstein and Churchill 1996, Yang 1995, Siepel and Haussler 2005). PhastCons uses a two-state phylo-HMM, with a state for conserved regions and a state for non-conserved regions. The value plotted at each site is the posterior probability that the corresponding alignment column was "generated" by the conserved state of the phylo-HMM. These scores reflect the phylogeny (including branch lengths) of the species in question, a continuous-time Markov model of the nucleotide substitution process, and a tendency for conservation levels to be autocorrelated along the genome (i.e., to be similar at adjacent sites). The general reversible (REV) substitution model was used. Note that, unlike many conservation-scoring programs, phastCons does not rely on a sliding window of fixed size, so short highly-conserved regions and long moderately conserved regions can both obtain high scores. More information about phastCons can be found in Siepel et al. (2005).

PhastCons currently treats alignment gaps as missing data, which sometimes has the effect of producing undesirably high conservation scores in gappy regions of the alignment. We are looking at several possible ways of improving the handling of alignment gaps.

Credits

This track was created at UCSC using the following programs:

  • Lastz (formerly Blastz) and multiz by Minmei Hou, Scott Schwartz and Webb Miller of the Penn State Bioinformatics Group.
  • AxtBest, axtChain, chainNet, netSyntenic, and netClass by Jim Kent at UCSC.
  • PhastCons by Adam Siepel at Cornell University.
  • "Wiggle track" plotting software by Hiram Clawson at UCSC.

The phylogenetic tree is based on the Saccharomyces Phylogeny page from the Department of Genetics at Washington University in St. Louis.

References

Phylo-HMMs and phastCons:

Felsenstein J, Churchill GA. A Hidden Markov Model approach to variation among sites in rate of evolution. Mol Biol Evol. 1996 Jan;13(1):93-104. PMID: 8583911

Siepel A, Bejerano G, Pedersen JS, Hinrichs AS, Hou M, Rosenbloom K, Clawson H, Spieth J, Hillier LW, Richards S, et al. Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes. Genome Res. 2005 Aug;15(8):1034-50. PMID: 16024819; PMC: PMC1182216

Siepel A, Haussler D. Phylogenetic Hidden Markov Models. In: Nielsen R, editor. Statistical Methods in Molecular Evolution. New York: Springer; 2005. pp. 325-351.

Yang Z. A space-time process model for the evolution of DNA sequences. Genetics. 1995 Feb;139(2):993-1005. PMID: 7713447; PMC: PMC1206396

Chain/Net:

Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D. Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9. PMID: 14500911; PMC: PMC208784

Multiz:

Blanchette M, Kent WJ, Riemer C, Elnitski L, Smit AF, Roskin KM, Baertsch R, Rosenbloom K, Clawson H, Green ED, et al. Aligning multiple genomic sequences with the threaded blockset aligner. Genome Res. 2004 Apr;14(4):708-15. PMID: 15060014; PMC: PMC383317

Lastz (formerly Blastz):

Chiaromonte F, Yap VB, Miller W. Scoring pairwise genomic sequence alignments. Pac Symp Biocomput. 2002:115-26. PMID: 11928468

Harris RS. Improved pairwise alignment of genomic DNA. Ph.D. Thesis. Pennsylvania State University, USA. 2007.