Human Gene NDST1 (ENST00000261797.7) from GENCODE V44
Description: Homo sapiens N-deacetylase and N-sulfotransferase 1 (NDST1), transcript variant 1, mRNA. (from RefSeq NM_001543) RefSeq Summary (NM_001543): This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]. Gencode Transcript: ENST00000261797.7 Gencode Gene: ENSG00000070614.15 Transcript (Including UTRs) Position: hg38 chr5:150,508,131-150,558,211 Size: 50,081 Total Exon Count: 15 Strand: + Coding Region Position: hg38 chr5:150,521,255-150,553,332 Size: 32,078 Coding Exon Count: 14
ID:NDST1_HUMAN DESCRIPTION: RecName: Full=Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; EC=2.8.2.8; AltName: Full=Glucosaminyl N-deacetylase/N-sulfotransferase 1; Short=NDST-1; AltName: Full=N-heparan sulfate sulfotransferase 1; Short=N-HSST 1; AltName: Full=[Heparan sulfate]-glucosamine N-sulfotransferase 1; Short=HSNST 1; Includes: RecName: Full=Heparan sulfate N-deacetylase 1; EC=3.-.-.-; Includes: RecName: Full=Heparan sulfate N-sulfotransferase 1; EC=2.8.2.-; FUNCTION: Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response. CATALYTIC ACTIVITY: 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=13.3 uM for K5 polysaccharide; KM=0.35 uM for N-acetylated HS-II; PATHWAY: Glycan metabolism; heparan sulfate biosynthesis. PATHWAY: Glycan metabolism; heparin biosynthesis. SUBUNIT: Monomer. SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein. TISSUE SPECIFICITY: Widely expressed. Expression is most abundant in heart, liver and pancreas. MISCELLANEOUS: The presence of 4 different heparan sulfate N- deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/sulfation reactions, whereas other later on fill in or extend already modified heparan sulfate sequences. SIMILARITY: Belongs to the sulfotransferase 1 family. NDST subfamily. WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="http://riodb.ibase.aist.go.jp/rcmg/ggdb/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P52848
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
Sequence and Links to Tools and Databases 
Microarray Expression Data 
