J Cell Biol 2006,
PMID: 16702231
Suetsugu, Shiro; Kurisu, Shusaku; Oikawa, Tsukasa; Yamazaki, Daisuke; Oda, Atsushi; Takenawa, Tadaomi
WAVE2 activates the actin-related protein (Arp) 2/3 complex for Rac-induced actin polymerization during lamellipodium formation and exists as a large WAVE2 protein complex with Sra1/PIR121, Nap1, Abi1, and HSPC300. IRSp53 binds to both Rac and Cdc42 and is proposed to link Rac to WAVE2. We found that the knockdown of IRSp53 by RNA interference decreased lamellipodium formation without a decrease in the amount of WAVE2 complex. Localization of WAVE2 at the cell periphery was retained in IRSp53 knockdown cells. Moreover, activated Cdc42 but not Rac weakened the association between WAVE2 and IRSp53. When we measured Arp2/3 activation in vitro, the WAVE2 complex isolated from the membrane fraction of cells was fully active in an IRSp53-dependent manner but WAVE2 isolated from the cytosol was not. Purified WAVE2 and purified WAVE2 complex were activated by IRSp53 in a Rac-dependent manner with PIP(3)-containing liposomes. Therefore, IRSp53 optimizes the activity of the WAVE2 complex in the presence of activated Rac and PIP(3).
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Text Mining Data
actin related protein (Arp) 2/3 → WAVE2: "
WAVE2 activates the
actin related protein (Arp) 2/3 complex for Rac induced actin polymerization during lamellipodium formation and exists as a large WAVE2 protein complex with Sra1/PIR121, Nap1, Abi1, and HSPC300
"
WAVE2 complex — IRSp53: "
When we measured Arp2/3 activation in vitro, the WAVE2 complex isolated from the membrane fraction of cells was fully active in an IRSp53 dependent manner but WAVE2 isolated from the cytosol was not
"
WAVE2 → IRSp53: "
Purified WAVE2 and purified WAVE2 complex were activated by IRSp53 in a Rac dependent manner with PIP ( 3 ) -containing liposomes
"
WAVE2 — Rac: "
Purified WAVE2 and purified WAVE2 complex were activated by IRSp53 in a Rac dependent manner with PIP ( 3 ) -containing liposomes
"
IRSp53 — Rac: "
Purified WAVE2 and purified WAVE2 complex were activated by IRSp53 in a Rac dependent manner with PIP ( 3 ) -containing liposomes
"
WAVE2 complex → PIP: "
Therefore, IRSp53 optimizes the activity of the WAVE2 complex in the presence of activated Rac and PIP ( 3 )
"
WAVE2 complex → Rac: "
Therefore, IRSp53 optimizes the activity of the WAVE2 complex in the presence of activated Rac and PIP ( 3 )
"
Manually curated Databases
-
NCI Pathway Database RAC1 signaling pathway:
None
→
RAC1/GTP complex (RAC1)
(modification, activates)
Evidence: assay, physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
None
→
IRSP53 (BAIAP2)
(modification, activates)
Evidence: assay, physical interaction
-
NCI Pathway Database RAC1 signaling pathway:
RAC1/GTP complex (RAC1)
→
IRSP53 (BAIAP2)
(modification, activates)
Evidence: assay, physical interaction
-
Reactome Reaction:
RAC1
→
BAIAP2
(reaction)
-
Reactome Reaction:
BRK1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
NCKAP1
→
BAIAP2
(reaction)
-
Reactome Reaction:
RAC1
→
CYFIP2
(indirect_complex)
-
Reactome Reaction:
NCKAP1
→
RAC1
(indirect_complex)
-
Reactome Reaction:
WASF2
→
RAC1
(indirect_complex)
-
Reactome Reaction:
CYFIP2
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
ABI1
→
BAIAP2
(reaction)
-
Reactome Reaction:
NCKAP1
→
RAC1
(reaction)
-
Reactome Reaction:
NCKAP1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
BRK1
→
BAIAP2
(reaction)
-
Reactome Reaction:
WASF2
→
RAC1
(reaction)
-
Reactome Reaction:
RAC1
→
ABI1
(reaction)
-
Reactome Reaction:
RAC1
→
ABI1
(indirect_complex)
-
Reactome Reaction:
RAC1
→
BRK1
(reaction)
-
Reactome Reaction:
RAC1
→
BRK1
(indirect_complex)
-
Reactome Reaction:
WASF2
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
WASF2
→
BAIAP2
(reaction)
-
Reactome Reaction:
CYFIP2
→
BAIAP2
(reaction)
-
Reactome Reaction:
RAC1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
ABI1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
RAC1
→
CYFIP2
(reaction)
In total, 11 gene pairs are associated to this article in curated databases