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LPA — PAK1

Text-mined interactions from Literome

Schmitz et al., Biochem Biophys Res Commun 2002 : In the present report we evaluated stimulation of PAK by LPA in rat aortic vascular smooth muscle cells ( VSMC ) by PAK immunocomplex MBP in-gel kinase assay ... LPA increased PAK activity 3-fold, peaking at 5 min and showing sustained activation up to 45 min. Inhibition of tyrosine kinases by pretreatment of VSMC with genistein or specific inhibition of Src by PP1 greatly diminished LPA induced PAK activation, whereas specific inhibition of PDFG- and EGF receptor kinase by tyrphostin AG1296 and AG1478 had no effect ... Furthermore, inhibition of Galpha ( i ) by pertussis toxin and inhibition of NADH/NADPH oxidase by diphenylene iodonium also diminished LPA induced stimulation of PAK ... In VSMC, PAK activation by LPA is mediated by Galpha ( i ) and is dependent on Src, whereas EGF- or PDGF receptor transactivation are not involved ... Furthermore, generation of ROS is required for LPA induced activation of PAK
Menard et al., Cell Signal 2003 : In this study, we show that PAK1 can be stimulated by carbachol, lysophosphatidic acid (LPA) , epidermal growth factor (EGF), and phorbol 12-myristate 13-acetate ( PMA ) by multiple independent and overlapping pathways ... Activation of PAK1 by LPA was independent of PI3-kinase and the EGF receptor, but was inhibited by dominant negative RhoA
Jung et al., Eur J Biochem 2004 (Melanoma) : Treatment of human melanoma cells A2058 with LPA increased phosphorylation and activation of PAK1, which was blocked by treatment with pertussis toxin and by inhibition of phosphoinositide 3-kinase (PI3K) with an inhibitor LY294002 or by overexpression of catalytically inactive mutant of PI3Kgamma, indicating that LPA induced PAK1 activation was mediated via a Gi protein and the PI3Kgamma signaling pathway ... In addition, we demonstrated that Rac1/Cdc42 signals acted as upstream effector molecules of LPA induced PAK activation ... However, Rho associated kinase, MAP kinase kinase 1/2 or phospholipase C might not be involved in LPA induced PAK1 activation or cell motility stimulation
Lee et al., Exp Cell Res 2005 : Finally, dominant negative mutant of Rho ( N19Rho ) -transfected cells did not affect PAK activation, while the cells stably transfected with p85 beta-PIX siRNA or N17Rac1 showed the reduction of LPA induced PAK activation