◀ Back to INS

INS — SLC12A3

Text-mined interactions from Literome

Sohara et al., PloS one 2011 : Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney ... This study examined whether insulin regulates NCC phosphorylation ... In cultured mpkDCT cells, insulin increased phosphorylation of STE20/SPS1 related proline-alanine-rich kinase ( SPAK ) and NCC in a dose dependent manner ... This insulin induced phosphorylation of NCC was suppressed in WNK4 and SPAK knockdown cells ... In addition, Ly294002, a PI3K inhibitor, decreased the insulin effect on SPAK and NCC phosphorylation, indicating that insulin induces phosphorylation of SPAK and NCC through PI3K and WNK4 in mpkDCT cells ... Time-course experiments in mpkDCT cells and mice suggested that SPAK is upstream of NCC in this insulin induced NCC phosphorylation mechanism, which was confirmed by the lack of insulin induced NCC phosphorylation in SPAK knockout mice ... Moreover, insulin administration to WNK4 hypomorphic mice did not increase phosphorylation of OSR1, SPAK and NCC in the kidney, suggesting that WNK4 is also involved in the insulin induced OSR1, SPAK and NCC phosphorylation mechanism in vivo
Chávez-Canales et al., J Hypertens 2013 : Insulin increases the functional activity of the renal NaCl cotransporter ... However, the effect of insulin on NCC functional activity has not been reported ... Using a heterologous expression system of Xenopus laevis oocytes, a mouse distal convoluted cell line, mDCT15 cells, endogenously expressing NCC, and an ex-vivo kidney perfusion technique, we assessed the effect of insulin on the activity and phosphorylation of NCC ... In Xenopus oocytes insulin increases the activity of NCC together with its phosphorylation at threonine residue 58 ... Activation of NCC by insulin was also observed in mDCT15 cells ... Additionally, insulin increased the NCC phosphorylation in kidney under the ex-vivo perfusion technique ... In oocytes and mDCT15 cells, insulin effect on NCC was prevented with inhibitors of phosphatidylinositol 3-kinase (PI3K), mTORC2, and AKT1 kinases, but not by inhibitors of MAP or mTORC1 kinases, suggesting that PI3K-mTORC2-AKT1 is the intracellular pathway required ... Additionally, activation of NCC by insulin was not affected by wild-type or mutant versions of with no lysine kinase 1, with no lysine kinase 4, or serum glucocorticoid kinase 1, but it was no longer observed in the presence of wild-type or the dominant negative, catalytically inactive with no lysine kinase 3, implicating this kinase in the process ... Insulin induces activation and phosphorylation of NCC