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SMAD4 — SP1

Pathways - manually collected, often from reviews:

• FastForward regulation: SP1 → SMAD4 (transcriptional regulation, unknown)
  Evidence: DNABINDING
• KEGG TGF-beta signaling pathway: Complex of SMAD2-SMAD3-SMAD4 → Complex of CREBBP-EP300-SP1 (protein-protein, activation)
  
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4 complex (SMAD3-SMAD4) → SP1 (SP1) (modification, collaborate) Lai et al., J Biol Chem 2000
  Evidence: physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4 complex (SMAD3-SMAD4) → SMAD3/SMAD4/SP1 complex (SMAD3-SMAD4-SP1) (modification, collaborate)
  Lai et al., J Biol Chem 2000
  Evidence: physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SP1 (SP1) → SMAD3/SMAD4/SP1 complex (SMAD3-SMAD4-SP1) (modification, collaborate)
  Lai et al., J Biol Chem 2000
  Evidence: physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4/ER alpha complex (SMAD3-SMAD4-ESR1) → SMAD3/SMAD4/SP1 complex (SMAD3-SMAD4-SP1) (transcription, inhibits)
  Poncelet et al., J Biol Chem 2001*, Matsuda et al., J Biol Chem 2001
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: AP1 complex (FOS-JUN) → SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1) (transcription, activates)
  Brodin et al., J Biol Chem 2000
  Evidence: mutant phenotype, reporter gene, physical interaction, other species
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4 complex (SMAD3-SMAD4) → SP1 (SP1) (modification, collaborate) Pardali et al., J Biol Chem 2000*
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4 complex (SMAD3-SMAD4) → SMAD3/SMAD4/SP1 complex (SMAD3-SMAD4-SP1) (modification, collaborate)
  Pardali et al., J Biol Chem 2000*
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SP1 (SP1) → SMAD3/SMAD4/SP1 complex (SMAD3-SMAD4-SP1) (modification, collaborate)
  Pardali et al., J Biol Chem 2000*
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17) → SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1) (modification, collaborate)
  Seoane et al., Nat Cell Biol 2001
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17) → MIZ-1 (ZBTB17) (modification, collaborate) Seoane et al., Nat Cell Biol 2001
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1) → MIZ-1 (ZBTB17) (modification, collaborate) Seoane et al., Nat Cell Biol 2001
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: MYC/MIZ-1 complex (MYC-ZBTB17) → SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17) (transcription, inhibits)
  Feng et al., EMBO J 2000, Seoane et al., Nat Cell Biol 2001, Matsuura et al., Nature 2004
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4 complex (SMAD3-SMAD4) → SMAD2-3/SMAD4/SP1/MIZ-1 complex (SMAD2_SMAD3-SMAD4-SP1-ZBTB17) (transcription, inhibits)
  Feng et al., EMBO J 2000, Seoane et al., Nat Cell Biol 2001, Matsuura et al., Nature 2004
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database HIF-1-alpha transcription factor network: HIF1A/ARNT/SMAD3/SMAD4/SP1 complex (HIF1A-ARNT-SMAD3-SMAD4-SP1) → SMAD3/SMAD4 complex (SMAD3-SMAD4) (modification, collaborate)
  Sánchez-Elsner et al., J Biol Chem 2002
  Evidence: physical interaction
• NCI Pathway Database HIF-1-alpha transcription factor network: HIF1A/ARNT/SMAD3/SMAD4/SP1 complex (HIF1A-ARNT-SMAD3-SMAD4-SP1) → SP1 (SP1) (modification, collaborate) Sánchez-Elsner et al., J Biol Chem 2002
  Evidence: physical interaction
• NCI Pathway Database HIF-1-alpha transcription factor network: HIF1A/ARNT/SMAD3/SMAD4/SP1 complex (HIF1A-ARNT-SMAD3-SMAD4-SP1) → HIF1A/ARNT complex (HIF1A-ARNT) (modification, collaborate)
  Sánchez-Elsner et al., J Biol Chem 2002
  Evidence: physical interaction
• NCI Pathway Database HIF-1-alpha transcription factor network: SMAD3/SMAD4 complex (SMAD3-SMAD4) → SP1 (SP1) (modification, collaborate) Sánchez-Elsner et al., J Biol Chem 2002
  Evidence: physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD3/SMAD4/SP1-3 complex (SMAD3-SMAD4-SP3_SP1) → beta5 Integrin (ITGB5) (transcription, activates) Lai et al., J Biol Chem 2000
  Evidence: mutant phenotype, reporter gene, physical interaction, other species
• NCI Pathway Database Validated targets of C-MYC transcriptional repression: SMAD2-3/SMAD4/MYC/Max/MIZ-1 complex (MYC-MAX-ZBTB17-SMAD2_SMAD3-SMAD4) → SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1) (transcription, inhibits)
  Staller et al., Nat Cell Biol 2001, Seoane et al., Nat Cell Biol 2001, Feng et al., Mol Cell 2002
  Evidence: mutant phenotype, reporter gene, physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SP1 (SP1) → SMAD2-3/SMAD4 complex (SMAD2_SMAD3-SMAD4) (modification, collaborate)
  Feng et al., EMBO J 2000
  Evidence: physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SP1 (SP1) → SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1) (modification, collaborate)
  Feng et al., EMBO J 2000
  Evidence: physical interaction
• NCI Pathway Database Regulation of nuclear SMAD2/3 signaling: SMAD2-3/SMAD4 complex (SMAD2_SMAD3-SMAD4) → SMAD2-3/SMAD4/SP1 complex (SMAD2_SMAD3-SMAD4-SP1) (modification, collaborate)
  Feng et al., EMBO J 2000
  Evidence: physical interaction
• Reactome Reaction: SMAD4 → SP1 (indirect_complex) Feng et al., EMBO J 2000
• Reactome Reaction: SMAD4 → SP1 (reaction) Feng et al., EMBO J 2000

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: SMAD4 — SP1 (physical association, affinity chromatography technology) Jungert et al., Carcinogenesis 2006*
• IRef Biogrid Interaction: SMAD4 — SP1 (direct interaction, pull down) Pardali et al., J Biol Chem 2000*
• IRef Biogrid Interaction: SMAD4 — SP1 (physical association, affinity chromatography technology) Luciakova et al., Biochem J 2008*
• IRef Biogrid Interaction: SMAD4 — SP1 (direct interaction, two hybrid) Pardali et al., J Biol Chem 2000*
• IRef Biogrid Interaction: SMAD4 — SP1 (physical association, affinity chromatography technology) Chung et al., Am J Respir Cell Mol Biol 2012*
• MIPS CORUM SMAD3-SMAD4-SP1 complex: SMAD3-SMAD4-SP1 complex complex (SMAD3-SMAD4-SP1) Botella et al., J Biol Chem 2001*
• IRef Corum Interaction: Complex of SMAD4-SMAD4-SP1-SMAD3-SP1-SMAD3 (association, coimmunoprecipitation) Botella et al., J Biol Chem 2001*
• IRef Hprd Interaction: SP1 — SMAD4 (in vivo) Pardali et al., J Biol Chem 2000*, Poncelet et al., J Biol Chem 2001*
• IRef Hprd Interaction: Complex of SP1-SMAD4-SMAD3-SMAD4-SP1-SMAD3-SMAD3-SP1-SMAD4 (in vivo) Botella et al., J Biol Chem 2001*
• IRef Intact Interaction: SMAD4 — SP1 (physical association, anti bait coimmunoprecipitation) Trinh et al., Oncogene 2011*
• IRef Ophid Interaction: SMAD4 — SP1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005
• IRef Ophid Interaction: SMAD4 — SP1 (aggregation, confirmational text mining) Botella et al., J Biol Chem 2001*

Text-mined interactions from Literome

Poncelet et al., J Biol Chem 2001 : In a Gal4-LUC reporter assay system, Sp1 stimulated the TGF-beta1 induced transcriptional activity of Gal4-Smad3, Gal4-Smad4 ( 266 ), or both
Jungert et al., Carcinogenesis 2006 (Pancreatic Neoplasms) : Moreover, inhibition of Sp1-DNA binding or transfection of Sp1-specific siRNA prevents TGFbeta induced Smad7 expression and consequently enhances Smad signaling in pancreatic cancer cells, as indicated by increased receptor mediated phosphorylation of Smad3