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CCL2 — TNF

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: CCL2 → TNF (increases) Newton et al., J Pharmacol Exp Ther 2007*
  Evidence: Similar levels of RANTES and MCP-1 release were induced by IL-1 and TNF treatments. However, the induction of IL-6, IL-8, and GMCSF by TNF was 50 to 80% lower than for IL-1, whereas TNF-induced GRO was some 35- to 40-fold lower than that for IL-1. In all instances, preincubation with PS-1145 or ML120B resulted in a significant attenuation of cytokine release (Fig. 6).
• OpenBEL Selventa BEL large corpus: CCL2 → TNF (increases)
  Evidence: In an important study by Weber et al. (1999), it was revealed that tumor necrosis factor (TNF)-a-treated endothelium expresses both the CXC chemokine Groa and the CC chemokine MCP-1.
• OpenBEL Selventa BEL large corpus: CCL2 → TNF (increases) Nanki et al., J Immunol 2001*
  Evidence: On the other hand, CCL2, CCL5, and CXCL12 were expressed on RA FLS, and their production was regulated by TNF-alpha, IL-1beta, and TGF-beta1.

Text-mined interactions from Literome

Mo et al., Exp Eye Res 1999 (Uveitis) : In the present study, we investigated the role of IL-8 and MCP-1 and regulation of these chemokines by TNFalpha and IL-1 in LPS induced uveitis in rabbits
Yamashiro et al., J Leukoc Biol 1999 : MCP-1 is selectively expressed in the late phase by cytokine stimulated human neutrophils : TNF-alpha plays a role in maximal MCP-1 mRNA expression ... Recombinant tumor necrosis factor alpha (TNF-alpha) induced a low level MCP-1 mRNA accumulation in neutrophils, and addition of anti-TNF-alpha IgG blocked 30-70 % of MCP-1 mRNA expression induced with PHA-sup
Weber et al., Blood 1999 : We found that overexpression of IkappaB-alpha in endothelial cells using a recombinant adenovirus prevented tumor necrosis factor-alpha (TNF-alpha) induced degradation of IkappaB-alpha and suppressed the upregulation of vascular cell adhesion molecule-1 ( VCAM-1 ), intercellular adhesion molecule-1 ( ICAM-1 ), and E-selectin mRNA and surface protein expression and the upregulation of transcripts for the chemokines monocyte chemoattractant protein 1 ( MCP-1 ) and growth related activity-alpha ( GRO-alpha ) by TNF-alpha
Barrett et al., Am J Physiol 1999 : The results support the hypothesis that TNF-alpha mediates cristobalite induced MCP-1 and MIP-2 expression through the generation of ROS
Bian et al., Curr Eye Res 1999 : It has been shown in this and other laboratories that interleukin-1 beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) are potent inducers of HRPE IL-8 and MCP-1 secretion ... IL-1beta and TNF-alpha induced dose dependent increases in HRPE IL-8 and MCP-1 secretion with maximal stimulation observed at 2-5 ng/ml. IL-4 alone ( 100 ng/ml ) resulted in a slight increase of MCP-1 and IL-8 secretion ... HRPE IL-8 and MCP-1 gene expression and protein production are stimulated by IL-1beta or TNF-alpha through pathways differentially modulated by IL-4 and GM-CSF
Murao et al., FEBS Lett 1999 : In human vascular endothelial cells, interleukin-1beta and tumor necrosis factor-alpha induced endogenous monocyte chemoattractant protein-1 protein secretion, mRNA expression and promoter activity
Dekkers et al., Infect Immun 1999 (Endotoxemia) : Divergent roles of tumor necrosis factor and platelet activating factor in endotoxin induced release of monocyte chemoattractant protein 1 and macrophage inflammatory protein 1beta in chimpanzees
Ping et al., J Biol Chem 1999 : These results show that the mechanisms of PDGF-BB and TNF activation of MCP-1 are distinct, although they both require the proximal regulatory region Sp1 binding site
Ortego et al., Atherosclerosis 1999 (Coronary Artery Disease...) : Ang II and TNF-alpha induced the expression of IP-10 ( 1.5 and 3.4-fold ) and MCP-1 ( 2.4 and 4-fold ) in VSMC
Murugesan et al., J Biomed Mater Res 2000 : TNFalpha induced EC expression and activity of MCP-1 and tissue factor and suppressed that of thrombomodulin on all substrates ... TNFalpha increased MCP-1 secretion significantly in aortic and pulmonary artery EC but to a lesser extent in microvascular EC
Ping et al., J Biol Chem 2000 : Sp1 binding is critical for promoter assembly and activation of the MCP-1 gene by tumor necrosis factor ... The monocyte chemoattractant protein-1 gene ( MCP-1 ) is induced by the inflammatory cytokine tumor necrosis factor through the coordinate assembly of an NF-kappaB dependent distal regulatory region and a proximal region that has been suggested to bind Sp1 as well as other factors
De Keulenaer et al., Arterioscler Thromb Vasc Biol 2000 : Convergence of redox-sensitive and mitogen activated protein kinase signaling pathways in tumor necrosis factor-alpha mediated monocyte chemoattractant protein-1 induction in vascular smooth muscle cells ... Incubation of VSMCs with multiple antioxidants ( diphenylene iodonium, liposomal superoxide dismutase, catalase, N-acetylcysteine, dimethylthiourea, and pyrrolidine dithiocarbamate ) had no effect on TNF-alpha mediated MCP-1 upregulation ... In this study, we show that TNF-alpha induces a parallel phosphorylation of extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38 mitogen activated protein kinase ( p38MAPK ) and increases MCP-1 mRNA expression in cultured VSMCs. Inhibition of ERK1/2 but not p38MAPK caused a partial attenuation of MCP-1 induction ( 43+/-10 % inhibition ) ... Thus, parallel ROS dependent and ERK1/2 dependent pathways converge to regulate TNF-alpha induced MCP-1 gene expression in VSMCs
Netea et al., Eur J Immunol 2000 (Chlamydia Infections) : In contrast, synthesis of MCP-1 and MIP-1alpha was dependent on endogenous TNF , but not IL-1
Steube et al., Mol Cell Biol Res Commun 2000 (Leukemia) : Besides TPA, tumor necrosis factor-alpha (TNF alpha) also distinctively enhanced IL-8 and MCP-1 production
Ohta et al., Metabolism 2000 : Inhibitory effect of troglitazone on tumor necrosis factor alpha induced expression of monocyte chemoattractant protein-1 in human mesangial cells ... We found that TNF-alpha increased the secretion of MCP-1 by 55-fold versus the control and troglitazone significantly inhibited this TNF-alpha induced increase in MCP-1 secretion ( 49.3 % ) ... We demonstrated that alpha-tocopherol also inhibited TNF-alpha induced MCP-1 production in HMCs, although its effects were not as strong as troglitazone
Ohta et al., Diabetes Res Clin Pract 2000 : Inhibitory effect of troglitazone on TNF-alpha induced expression of monocyte chemoattractant protein-1 ( MCP-1 ) in human endothelial cells ... We found that TNF-alpha increased the secretions of MCP-1 119-fold vs. control, and that troglitazone significantly inhibited this TNF-alpha induced increase in MCP-1 secretions ( 19.4 % )
Gordon et al., Cell Immunol 2000 : Depletion of TGFbeta, TNFalpha , or both TGFbeta and TNFalpha from the mediator pool secreted by mast cells activated via the FcepsilonRI reduced the mast-cell-driven fibroblast MCP-1 response by 80+/-15, 56+/-11, or 82+/-5 %, respectively
Yamanaka et al., Int Arch Allergy Immunol 2000 (Leukemia, T-Cell...) : Regarding the biological functions of MCF-pl5-L other than MCA and chemotactic activity for alveolar macrophages, we found that MCF-pl5-L but not C5a and MCP-1 could prolong the life span of alveolar macrophages, probably by inhibiting apoptosis of macrophages, and stimulate the production of TNF-alpha from macrophages
Narumi et al., Cytokine 2000 : On the other hand, JE/MCP-1 mRNA expression was detected only in non-parenchymal cells in response to TNF-alpha and IL-1beta, but not in response to IFN-gamma
Andoh et al., Gastroenterology 2000 : IL-8 and MCP-1 secretion was rapidly induced by both IL-1beta and tumor necrosis factor (TNF)-alpha
Wetzler et al., Biochem Biophys Res Commun 2000 : In vitro studies confirmed a regulatory role of NO for keratinocyte derived chemokine expression, as NO attenuated IL-1beta- and TNF-alpha induced MCP-1 mRNA expression, whereas NO augmented IL-1beta induced IL-8 ( functional human homolog to murine MIP-2 ) mRNA expression in the human keratinocyte cell line HaCaT
Iseki et al., Biochem Biophys Res Commun 2000 : These results indicate that TNF-alpha dependent expression of MCP-1 in HASMC is tightly regulated by NF-kappaB and that intracellular copper level is crucial for the TNF-alpha dependent activation of NF-kappaB in HASMC
Li et al., J Oral Pathol Med 2000 : In contrast, TNF-alpha induced a more rapid increase in MCP-1 production by SK than OK, peak production occurring after 24 and 72 h, respectively
Murao et al., Biochem Biophys Res Commun 2000 : Although TNF-alpha stimulates MCP-1 expression and secretion, the mechanism by which TNF-alpha stimulates expression of the MCP-1 gene is not known ... In HUVECs, wortmannin, a PI3-kinase inhibitor, inhibits TNF-alpha mediated MCP-1 secretion at a dose dependent manner ... These findings show that Akt/PKB participates in the TNF-alpha induction of MCP-1 gene transcription in endothelial cells
Yang et al., J Am Soc Nephrol 2000 : Supernatant nitrite, monocyte chemoattractant protein-1 , and tumor necrosis factor-alpha protein levels also increased by 1.8-, 7.1-, and 5-fold, respectively
Goebeler et al., Blood 2001 : Both NiCl ( 2 ) - and TNFalpha induced MCP-1 synthesis was sensitive to D609, an inhibitor of phosphatidylcholine dependent phospholipase C ( PC-PLC ) ... Consistent with that finding, stimulation with NiCl ( 2 ) or TNFalpha activated IkappaB kinase-beta (IKKbeta), and transient transfection of dominant negative IKKbeta strongly inhibited NiCl ( 2 ) - and TNFalpha induced MCP-1 expression
Feldmann et al., Annu Rev Immunol 2001 (Arthritis, Rheumatoid...) : Clinical investigations in which the activity of TNF alpha in RA patients was blocked with intravenously administered infliximab, a chimeric anti-TNF alpha monoclonal antibody ( mAB ), has provided evidence that TNF regulates IL-6, IL-8, MCP-1 , and VEGF production, recruitment of immune and inflammatory cells into joints, angiogenesis, and reduction of blood levels of matrix metalloproteinases-1 and -3
Yoshida et al., Invest Ophthalmol Vis Sci 2001 : IL-4 and -13, but not IL-10, enhanced constitutive and TNF-alpha induced HRPE IL-8 and MCP-1 secretion ... IL-13 potentiated TNF-alpha induced MCP-1 , but not IL-8 secretion by cocultures
Damås et al., Basic Res Cardiol 2001 : We hypothesised that MCP-1 may directly contribute to an inflammatory response in the cardiomyocytes, and in the present study we examined in adult rat cardiomyocytes : ( i ) the effect of tumour necrosis factor (TNF)alpha on MCP-1 production, ( ii ) the effect of MCP-1 on production of other inflammatory cytokines, and ( iii ) if the anti-inflammatory cytokine interleukin (IL)-10 could suppress any TNFalpha induced MCP- 1 production ... ( iv ) Co-stim-ulation with IL-10 resulted in a approximately 55 % reduction in TNFalpha stimulated MCP-1 levels in cardiomyocyte culture supernatants
Hoeck et al., J Immunol 2001 : In the same experiments, TNF-alpha induced activation of the monocyte chemoattractant protein-1/CCL2 gene was independent of STAT6 transfection
Olszewski et al., Infect Immun 2001 (Cryptococcosis...) : C. neoformans 52D induced tumor necrosis factor alpha (TNF-alpha) , gamma interferon ( IFN-gamma ), and MCP-1 in the lungs of infected wild-type ( WT ) and MIP-1alpha knockout ( KO ) mice by day 7 postinfection
Lakshminarayanan et al., Am J Pathol 2001 (Ischemia) : MCP-1 could be induced by exogenous tumor necrosis factor (TNF)-alpha or by postischemic cardiac lymph containing TNF-alpha
Momoi et al., Chest 2001 (Adenocarcinoma...) : In A549 cells, interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha induced endogenous MCP-1 protein secretion and messenger RNA expression
Nanki et al., J Immunol 2001 (Arthritis, Rheumatoid) : On the other hand, CCL2 , CCL5, and CXCL12 were expressed on RA FLS, and their production was regulated by TNF-alpha , IL-1beta, and TGF-beta1
Oh et al., Atherosclerosis 2001 (Arteriosclerosis) : In culture, hematein also significantly inhibited the secretion of soluble VCAM-1 and of monocyte chemotactic protein-1 (MCP-1) respectively induced by tumor necrotic factor alpha (TNF-alpha) and mildly oxidized low density lipoprotein in human umbilical vein endothelial cell ( HUVEC ) culture
Zhang et al., FASEB J 2001 : LA also strongly inhibited TNF-alpha induced mRNA expression of monocyte chemoattractant protein-1 but did not affect expression of TNF-alpha receptor 1
Cattaruzza et al., J Biol Chem 2002 (Inflammation) : Antisense oligonucleotide suppression of zfm1 protein synthesis mimicked the stimulatory effects of IL-1 beta and TNF alpha on SMC proliferation and expression of the chemokine MCP-1 and the vascular cell adhesion molecule-1
Kohno et al., J Rheumatol 2001 : T-614 suppressed TNF-alpha induced production of IL-6, IL-8, and monocyte chemoattractant protein 1 , and also reduced the accumulation of IL-6 and IL-8 mRNA in a concentration dependent manner
Yang et al., Am J Gastroenterol 2002 (Colitis, Ulcerative) : Further, the secretion of interleukin 8, GROalpha, epithelial cell derived neutrophil activator 78, and monocyte chemotactic protein 1 was higher in UC patients than in normal controls, induced significantly by TNFalpha , and down-regulated by dexamethasone
Matejuk et al., J Neurosci Res 2002 (Encephalomyelitis, Autoimmune, Experimental) : Of note, we present evidence that TNF-alpha may be involved in regulating RANTES and MIP-1alpha, and that IL-4 may be involved in regulating MCP-1
Okuno et al., Scand J Gastroenterol 2002 : IL-1beta and TNF-alpha both induced a dose- and time dependent increase in IL-8, MCP-1 and MMP-1 secretion, and weakly stimulated MMP-2 secretion
Carpenter et al., BMC infectious diseases 2002 (Lung Neoplasms) : In this study, we examined the NF-kappaB mediated effects of RSV and the proinflammatory cytokine TNFalpha on the induction of IL-8, MCP-1 and RANTES chemokine gene expression in A549 epithelial cells
Loghmani et al., Inflammation 2002 : Our results show that pleural fluid isolated human fibroblasts release IL-8 and MCP-1 upon stimulation with IL-1 beta, TNF-alpha , and LPS in both a concentration- and time dependent manner ... TNF-alpha induced a 95-fold increase in IL-8 and an 84-fold increase in MCP-1 levels, as compared to the controls
Neumark et al., Immunol Lett 2002 (Adenocarcinoma...) : The expression of MCP-1 , IL-6 and MMPs in both DA3-high and CSML-high cells was up-regulated by tumor necrosis factor alpha (TNFalpha)
Biswas et al., Int Immunopharmacol 2002 : Inhibitor studies with genistein on MCP-1 induced macrophage TNF and IL-1 production additionally supported the role of protein tyrosine phosphorylation in the process of macrophage activation with MCP-1
Lee et al., Nephron 2002 : Analogue of cGMP ( 8-bromo-cGMP ) had no significant effect on TNF-alpha induced MCP-1 mRNA expression and guanylate cyclase inhibitor ( ODQ ) also had no significant influence on the inhibitory effect of SIN-1
Janssen et al., Nephron 2002 : The additional proinflammatory stimulus TNFalpha increased MCP-1 protein production in 30 as compared to 5 mM D-glucose primed HMC ( 2,194 +/- 568 vs. 1,422 +/- 379 pg MCP-1/10 ( 4 ) cells x ml in 30 vs. 5 mM D-glucose primed HMC +24 h TNFalpha 500 U/ml, p = 0.002 )
Sabatini et al., Immunol Lett 2002 (Asthma) : In contrast, a significant increase in MCP-1 and eotaxin release was observed in the presence of TNF-alpha ( P < 0.05 ) but not of IL-4 ( P > 0.05 )
Brueckmann et al., Thromb Haemost 2003 : After stimulation of MCP-1-mRNA-transcription with TNF-alpha ( 0.1-1 ng/ml ), HUVEC 's were washed and an inhibitor of gene transcription, Actinomycin D ( 1 micro g/ml ), was added in the presence or absence of APC
Boekhoudt et al., J Immunol 2003 : The induction of the monocyte chemoattractant protein 1 gene ( MCP-1 ) by TNF occurs through an NF-kappaB dependent distal regulatory region and an Sp1 dependent proximal regulatory region that are separated by 2.2 kb of sequence ... To investigate how these regions coordinate activation of MCP-1 in response to TNF , experiments were performed to examine the role of coactivators, changes in local chromatin structure, and the acetylation of histones at the MCP-1 regulatory regions
Gottstein et al., Br J Nutr 2003 (Arteriosclerosis) : Both isoflavones also dose-dependently decreased monocyte chemoattractant protein-1 secretion induced by TNF-alpha in human umbilical vein endothelial cells
Chiu et al., Chin J Physiol 2002 : Shear stress attenuates tumor necrosis factor-alpha induced monocyte chemotactic protein-1 expressions in endothelial cells ... Less inhibition of TNF-alpha induced MCP-1 mRNA expression was found in ECs pre exposed to a low level of shear stress ( 1.2 dynes/cm2 ) for 24 h as compared with the cells presheared ( pre exposed to shear stress ) for 4 h. Simultaneous exposure of ECs to TNF-alpha and a high or low level of shear stress down-regulated TNF-alpha induced MCP-1 gene expressions, suggesting that the post-flow condition modulates endothelial responses to cytokine stimulation
Luo et al., Glia 2003 (Disease Models, Animal) : Tumor necrosis factor is required for RANTES induced astrocyte monocyte chemoattractant protein-1 production
Gao et al., J Tongji Med Univ 1999 (Endometriosis...) : Effect of IL-1 beta and TNF-alpha on the expression of monocyte chemotactic protein-1 in endometriotic cells
Meng et al., J Tongji Med Univ 2000 : Interleukin-1 beta, tumor necrosis factor-alpha and lipopolysaccharide induce expression of monocyte chemoattractant protein-1 in calf aortic smooth muscle cells
Steube et al., Leuk Res 2003 (Leukemia, Promyelocytic, Acute) : Besides the staurosporines, also 12-O-tetradecanoyl phorbol acetate ( TPA ) and tumor necrosis factor-alpha (TNFalpha) strongly increased the IL-8 and MCP-1 secretion of NB-4 cells
Neumark et al., Int J Cancer 2003 (Acute Disease...) : Furthermore, additional results demonstrated that monocytic cell derived TNFalpha upregulated MCP-1 secretion from the tumor cells and that MCP-1 in turn promoted the secretion of TNFalpha from monocytic cells
Weber et al., J Leukoc Biol 2003 : ANP inhibits TNF-alpha induced endothelial MCP-1 expression -- involvement of p38 MAPK and MKP-1 ... The aim of this study was to determine whether ANP is able to inhibit TNF-alpha induced expression of monocyte chemoattractant protein-1 ( MCP-1 ) in endothelial cells and to elucidate the mechanisms involved ... Pretreatment of human umbilical vein endothelial cells ( HUVEC ) with ANP significantly reduced TNF-alpha induced expression of MCP-1 protein and mRNA ... Antisense experiments showed a requirement of MAPK phosphatase-1 (MKP-1) induction and therefore, inhibition of p38 MAPK in the ANP mediated inhibition of TNF-alpha induced expression of MCP-1
Chen et al., Am J Physiol Heart Circ Physiol 2004 : Superoxide, H2O2, and iron are required for TNF-alpha induced MCP-1 gene expression in endothelial cells : role of Rac1 and NADPH oxidase ... Adenovirus mediated expression of superoxide dismutase and catalase inhibited TNF-alpha induced MCP-1 gene expression, suggesting important roles of superoxide ( O ( 2 ) ( - ). ) and H ( 2 ) O ( 2 ) in MCP-1 gene activation ... In addition, the iron chelator 1,2-dimethyl-3-hydroxypyridin-4-one and the hydroxyl radical scavengers dimethylthiourea and dimethyl sulfoxide inhibited TNF-alpha induced MCP-1 expression, suggesting important roles of iron and hydroxyl radicals in inflammatory signal activation ... In contrast, scavenging of peroxynitrite with 5,10,15,20-tetrakis- ( 4-sulfonatophenyl ) prophyrinato iron ( III ) chloride had no effect on TNF-alpha induced MCP-1 expression ... generation, with diphenylene iodonium suppressed TNF-alpha induced MCP-1 mRNA accumulation ... Expression of dominant negative N17Rac1 by adenovirus suppressed TNF-alpha induced MCP-1 mRNA levels and MCP-1 protein secretion ... Expression of N17Rac1 inhibited TNF-alpha induced MCP-1 and NF-kappaB transcriptional activity ... These data suggest that ROS such as superoxide and H ( 2 ) O ( 2 ) derived from Rac1 activated NADPH oxidase mediate TNF-alpha induced MCP-1 expression in endothelial cells
Murugesan et al., J Mol Cell Cardiol 2003 : LPC did not augment TNFalpha induction of MCP-1 or IL-8
Juarranz et al., Rheumatology (Oxford) 2004 (Arthritis, Rheumatoid...) : The effects of VIP on basal or tumour necrosis factor alpha (TNF-alpha) stimulated production of CCL2 ( MCP-1, monocyte chemotactic protein 1 ), CXCL8 [ interleukin (IL)-8 ], IL-6 and TNF-alpha were studied by specific ELISAs ( enzyme linked immunosorbent assays )
Chua et al., Cell Mol Biol Incl Cyto Enzymol 2003 (Mucocutaneous Lymph Node Syndrome) : Using semi-quantitative RT-PCR and cell based ELISA, we demonstrated that tumor necrosis factor-a induced the expression of intercellular adhesion molecules-1 and E-selectin, as well as monocyte chemoattractant protein-1 , in a time- and dose dependent manner in primary human coronary artery endothelial cell cultures
Park et al., Nephrol Dial Transplant 2004 : Dexamethasone regulates AP-1 to repress TNF-alpha induced MCP-1 production in human glomerular endothelial cells ... TNF-alpha ( 10 ng/ml ) increased MCP-1 mRNA expression in HGEC and also the release of MCP-1 protein into culture media ... TNF-alpha induced MCP-1 production appeared to be NF-kappa B and AP-1 interdependent, based on the following results ... These data demonstrate that while TNF-alpha induced MCP-1 production is mediated by the cooperative action of NF-kappa B and AP-1 in HGEC, dexamethasone represses TNF-alpha induced MCP-1 production via suppression of AP-1 binding activity
Rahimi et al., Am J Transplant 2004 : TNF-alpha augmented IgG1 induced secretion of MCP-1 and KC
Chen et al., J Pharmacol Exp Ther 2004 : Dual regulation of tumor necrosis factor-alpha induced CCL2/monocyte chemoattractant protein-1 expression in vascular smooth muscle cells by nuclear factor-kappaB and activator protein-1 : modulation by type III phosphodiesterase inhibition ... Our results showed that tumor necrosis factor (TNF)-alpha induced a marked increase in CCL2/MCP-1 production in dose- and time dependent manners ... 2- ( 2-Amino-3-methoxyphenyl ) -4H-1-benzopyran-4-one ( PD98059 ), 1,4-diamino-2,3-dicyano-1,4-bis ( 2-aminophenylthio ) butadiene ( U0126 ) [ both inhibitors of p42/44 mitogen activated protein kinase ( MAPK ) kinase ], and anthra [ 1hyphen ] 9-cd ] pyrazol-6 ( 2H ) -one ( SP600125 ) [ an inhibitor of c-Jun NH ( 2 ) -terminal kinases ( JNKs ) ] attenuated TNF-alpha induced CCL2/MCP-1 production, without affecting I-kappaBalpha degradation or p65/nuclear factor-kappaB (NF-kappaB) nuclear translocation ... The NF-kappaB inhibitor carbobenzoxy-l-leucyl-l-leucyl-l-leucinal ( MG132 ) attenuated TNF-alpha induced CCL2/MCP-1 production in the presence of increased phospho-JNK and phospho-c-Jun levels ... When SP600125 was added simultaneously, MG132 completely inhibited TNF-alpha induced CCL2/MCP-1 production ... Finally, the pretreatment of VSMCs with PTX or cilostamide, but not denbufylline, reduced TNF-alpha induced CCL2/MCP-1 production, which was preceded by attenuation of p65/NF-kappaB nuclear translocation, p42/44 MAPK, and JNK-c-Jun phosphorylation, and c-Fos up-regulation ... These data indicate that TNF-alpha stimulated CCL2/MCP-1 production in rat VSMCs is dually regulated by activator protein-1 (AP-1) and NF-kappaB pathways, and inhibition of type III phosphodiesterase contributes substantially to the suppressive effect of PTX on CCL2/MCP-1 production via down-regulation of AP-1 and NF-kappaB signals
Yamagishi et al., Drugs Exp Clin Res 2003 : Nifedipine inhibits tumor necrosis factor-alpha induced monocyte chemoattractant protein-1 overexpression by blocking NADPH oxidase mediated reactive oxygen species generation ... In this study, we investigated whether nifedipine, one of the most popular DHPs, could inhibit tumor necrosis factor-alpha (TNF-alpha) induced reactive oxygen species ( ROS ) generation and subsequent monocyte chemoattractant protein-1 ( MCP-1 ) expression in human umbilical vein endothelial cells ( HUVEC ) ... The results demonstrate that nifedipine could inhibit TNF-alpha induced MCP-1 overexpression in HUVEC by suppressing NADPH oxidase mediated ROS generation
Yang et al., Atherosclerosis 2004 (Arteriosclerosis) : Aspirin at the dose as low as 10 microg/ml significantly inhibited the release of TNF stimulated MCP-1 by 29.1 % ( P = 0.008 ) and IL-8 by 26.9 % ( P = 0.0146 ) as compared to TNF stimulated release
Bian et al., Invest Ophthalmol Vis Sci 2004 : The IC ( 50 ) for inhibition of MCP-1 secretion induced by IL-1beta, TNF-alpha , and hRPE-monocyte binding was 16, 12, and less than 3 micro M, respectively
Chen et al., Am J Physiol Heart Circ Physiol 2004 (Arteriosclerosis) : Sphingosine kinase-1 mediates TNF-alpha induced MCP-1 gene expression in endothelial cells : upregulation by oscillatory flow ... We further investigated the role of SphK1 in TNF-alpha induced expression of inflammatory genes, such as monocyte chemoattractant protein-1 ( MCP-1 ) and VCAM-1 by using small interfering RNA ( siRNA ) specifically for SphK1 ... Treatment of endothelial cells with SphK1 siRNA suppressed TNF-alpha induced increase in MCP-1 mRNA levels, MCP-1 protein secretion, and activation of p38 MAPK ... These data suggest that SphK1 mediates TNF-alpha induced MCP-1 gene expression through a p38 MAPK dependent pathway and may participate in oscillatory flow mediated proinflammatory signaling pathway in the vasculature
Onai et al., Cardiovasc Res 2004 (Myocardial Reperfusion Injury) : TNF-alpha induced production of interleukin-1beta and monocyte chemoattractant protein-1 from cultured cardiomyocytes was reduced significantly by IMD-0354
Watanabe et al., Am J Physiol Gastrointest Liver Physiol 2004 (Recurrence...) : TNF-alpha increased the number of macrophages and MCP-1 mRNA expression in scarred mucosa from 4 h, whereas it increased MPO activities ( marker of neutrophil infiltration ) and mRNA expression of MIP-2 and CINC-2alpha from 24 h. Anti-MCP-1 antibody inhibited leukocyte infiltration with reduction of the levels of C-X-C chemokines and prevented ulcer recurrence
Shi et al., Cell Biochem Biophys 2004 (Atherosclerosis) : We observed constitutive expression of interleukin (IL)-6, IL-8, granulocyte macrophage colony stimulating factor ( GM-CSF ), and monocyte chemoattractant protein (MCP)-1 in both human and baboon endothelial cells, and these cytokines were further induced by TNF-alpha and LPS
Harigai et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid...) : CD14+ RA synovial cells stimulated with rsCD154 plus interferon-gamma (IFNgamma) induced significantly higher production of IL-6, IL-8, and monocyte chemoattractant protein 1 by FLS compared with unstimulated CD14+ synovial cells, through TNFalpha- , IL-1alpha-, and IL-1beta mediated pathways
Kawano et al., Am J Reprod Immunol 2004 : Our data indicated that M-CSF and MCP-1 were regulated by IL-1alpha and TNF-alpha
Jiang et al., Free radical research 2004 : The mRNA accumulation of IL-8, MCP-1 , RANTES, and eotaxin, and activation of NF-kappaB were induced by TNF-alpha for 2 h ; all were suppressed significantly by the above two pretreatments
Nie et al., J Biol Chem 2005 : We reported here that the PPARgamma agonists 15-deoxy-Delta ( 12,14 ) -PGJ ( 2 ) ( 15d-PGJ ( 2 ) ) and troglitazone, but not PPARalpha agonist WY-14643, inhibited TNFalpha induced production of eotaxin and monocyte chemotactic protein-1 (MCP-1) but not IL-8
Yamagishi et al., Drugs Exp Clin Res 2004 : We have previously shown that nifedipine, one of the most popular dihydropyridine based calcium antagonists, blocked tumor necrosis factor-alpha induced monocyte chemoattractant protein-1 expression in endothelial cells ( ECs ) through its antioxidative properties
Takeshita et al., J Oral Sci 2005 (Second Messenger Systems) : Although TNF-alpha stimulated the AP-1 mediated expression of the monocyte chemoattractant JE/MCP-1 , this stimulation was inhibited by DHS
Yamagishi et al., Drugs Exp Clin Res 2005 : Nifedipine inhibits tumor necrosis factor-alpha induced upregulation of monocyte chemoattractant protein-1 mRNA levels by suppressing CD40 expression in endothelial cells ... These results demonstrate that nifedipine could inhibit the TNF-alpha induced upregulation of MCP-1 mRNA levels via suppression of CD40 expression in HUVEC
Hasan et al., BMC immunology 2005 (Tuberculosis...) : CCL2 is activated by TNFalpha and is essential for recruitment of monocytes and T cells to the site of mycobacterial infection
Masamune et al., Biochem Pharmacol 2005 : Ellagic acid inhibited platelet derived growth factor (PDGF)-BB induced proliferation and migration, interleukin (IL)-1beta- and tumor necrosis factor (TNF)-alpha induced monocyte chemoattractant protein-1 production, and expression of alpha-smooth muscle actin and collagen genes
Liu et al., Am J Respir Cell Mol Biol 2005 (Lung Diseases) : In vitro analysis indicates that alveolar cell expression of TNF-R2 is critical in the induction of epithelial monocyte chemoattractant protein (MCP)-1 expression specifically in response to soluble TNF-alpha , suggesting an important role for this receptor in bystander lung injury ... However, TNF-R2 was dispensable for induction of alveolar MCP-1 expression in response to transmembrane TNF-alpha expressed by antigen-specific CD8+ T cells, and the effects of the two receptors seem to be additive
Li et al., Biochem Biophys Res Commun 2005 : Hypoxia reduces constitutive and TNF-alpha induced expression of monocyte chemoattractant protein-1 in human proximal renal tubular cells ... Here, we investigated the regulatory effects of hypoxia on constitutive and TNF-alpha stimulated expression of monocyte chemoattractant protein-1 ( MCP-1 ) in cultured human proximal renal tubular cells ( HPTECs )
Calabrò et al., J Vasc Res 2005 : The two new PPAR-gamma agonists and 15d-PGJ2 also inhibited TNF-alpha induced interleukin-6 (IL-6) and monocyte chemoattractant protein-1 ( MCP-1 ) production in supernatants of TNF-alpha stimulated ECs, whereas ciglitazone and DIM-C-pPhCH ( 3 ) did not decrease TNF-alpha induced expression of these two proteins
Kimura et al., Rinsho Byori 2005 (Anoxia...) : In the present study, we examined the effects of hypoxia and TNF-alpha on PAI-1, MCP-1 and VEGF expression in cultured human proximal renal tubular cells ( HPTECs ) ... Hypoxia reduced basal and TNF-alpha stimulated MCP-1 expression, while it induced VEGF expression in HPTECs
Villiger et al., J Immunol 1992 (Synovitis) : Stimulation of synoviocytes with IL-1, TNF-alpha , LPS, platelet derived growth factor, and transforming growth factor-beta-1, but not with basic fibroblast growth factor causes a marked increase in MCP-1 mRNA levels
Kang et al., Eur J Pharmacol 2005 : TNF-alpha induced monocyte chemoattractant protein-1 ( MCP-1 ) expression was also attenuated by addition of PGG
Moon et al., Life Sci 2006 : TNF-alpha induced MCP-1 expression was also attenuated by the addition of AR
Masamune et al., J Cell Biochem 2006 : Curcumin inhibited platelet derived growth factor ( PDGF ) -induced proliferation, alpha-smooth muscle actin gene expression, interleukin-1beta- and tumor necrosis factor (TNF)-alpha induced MCP-1 production, type I collagen production, and expression of type I and type III collagen genes
Chen et al., Am J Physiol Heart Circ Physiol 2006 : Nrf2 suppressed TNF-alpha induced monocyte chemoattractant protein (MCP)-1 and VCAM-1 mRNA and protein expression in a dose dependent manner and inhibited TNF-alpha induced monocytic U937 cell adhesion to HAECs
Shun et al., Ann Otol Rhinol Laryngol 2005 (Nasal Polyps) : In this study, the effects of tumor necrosis factor alpha (TNF-alpha) on the expression of C-C chemokine ligand 2 (CCL2) in fibroblasts derived from nasal polyps ( NPFs ) were investigated ... The roles of cyclooxygenase (COX) 2 and prostaglandins in the mediation of TNF-alpha stimulated CCL2 gene expression were also investigated ... The Northern blot analysis showed that TNF-alpha stimulated the expression of CCL2 and COX-2 genes, and the synthesis of CCL2 messenger RNA was COX-2 dependent ... Curcumin ( AP-1 inhibitor ) markedly suppressed the TNF-alpha induced CCL2 expression
Yamamoto et al., Toxicol Appl Pharmacol 2006 (Pneumonia) : LTA produced a dose related increase in early pulmonary inflammation, which was characterized by ( 1 ) influx of polymorphonuclear neutrophils ( PMNs ) and ( 2 ) induction of interleukin (IL)-6, tumor necrosis factor (TNF)-alpha , macrophage inflammatory protein (MIP)-1alpha/CCL3, but no effect on monocyte chemoattractant protein (MCP)-1/CCL2 at 24 h after instillation
Cui et al., Ophthalmic Res 2006 : These findings indicate that berberine dose-dependently inhibited the expression of IL-8 and MCP-1 induced by IL-1beta or TNF-alpha
Bouchelouche et al., J Urol 2006 : We investigated whether HDSMCs are capable of expressing and releasing MCP-1 , IL-6 and SCF in response to IL-4, IL-13, IL-1beta and tumor necrosis factor-alpha ... Furthermore, IL-1beta and tumor necrosis factor-alpha alone induced significant release of MCP-1, IL-6 and SCF but in combination with IL-4 or IL-13 it induced greater secretion of MCP-1 , IL-6 and SCF
Lee et al., Atherosclerosis 2006 (Carotid Artery Diseases...) : In addition to fractalkine, ALA successfully inhibited TNF-alpha stimulated expression of vascular cell adhesion molecule-1 and monocyte chemotactic protein-1 in cultured VSMCs
Lockwood et al., Am J Pathol 2006 (Pre-Eclampsia) : Regulation of monocyte chemoattractant protein-1 expression by tumor necrosis factor-alpha and interleukin-1beta in first trimester human decidual cells : implications for preeclampsia
Wang et al., Pflugers Arch 2006 (Inflammation) : In contrast, TNF-alpha induced rapid and substantial increases in expression of the genes encoding IL-6, MCP-1 , NGF and TNF-alpha itself ; IL-6 and TNF-alpha mRNA levels peaked at 2 h with 75-fold and 600-fold increases, respectively ... The adipokine secretion pattern largely paralleled cellular mRNA levels ; IL-6 ( transiently ), MCP-1 , NGF and VEGF release were stimulated by TNF-alpha , with an accelerating rate of MCP-1 secretion over 24 h. TNF-alpha has rapid and substantial effects on the synthesis of key inflammation related adipokines in human adipocytes, with highly gene-specific responses
Bandyopadhyay et al., Biochem Pharmacol 2006 : In MC3T3-E1 cells, apigenin dose-dependently ( from 5 to 20 microM ) inhibits TNFalpha induced production of the osteoclastogenic cytokines, IL-6 ( interleukin-6 ), RANTES ( regulated upon activation, normal T cell expressed and -secreted ), monocyte chemoattractant protein-1 ( MCP-1 ) and MCP-3
Leonardi et al., Clin Exp Allergy 2006 (Conjunctivitis, Allergic) : IL-6, IL-8, MCP-1 , RANTES and eotaxin were detected from fibroblasts cultures, and were all up-regulated by TNF-alpha
Kanda et al., Biochem Pharmacol 2006 : Suppressive effects of antimycotics on tumor necrosis factor-alpha induced CCL27, CCL2 , and CCL5 production in human keratinocytes ... We examined in vitro effects of antimycotics on TNF-alpha induced CCL27, CCL2 , and CCL5 production in human keratinocytes ... Antimycotics ketoconazole and terbinafine hydrochloride suppressed TNF-alpha induced CCL27, CCL2 , and CCL5 secretion and mRNA expression in keratinocytes in parallel to the inhibition of NF-kappaB activity while fluconazole was ineffective ... Anti-prostaglandin E2 ( PGE2 ) antiserum or antisense oligonucleotides against PGE2 receptor EP2 or EP3 abrogated inhibitory effects of ketoconazole and terbinafine hydrochloride on TNF-alpha induced NF-kappaB activity and CCL27, CCL2 , and CCL5 production, indicating the involvement of endogenous PGE2 in the inhibitory effects ... Carboxyheptyl imidazole also suppressed TNF-alpha induced NF-kappaB activity and CCL27, CCL2 , and CCL5 production ... These results suggest that ketoconazole and terbinafine hydrochloride may suppress TNF-alpha induced NF-kappaB activity and CCL27, CCL2 , and CCL5 production by increasing PGE2 release from keratinocytes
Nanua et al., Am J Respir Cell Mol Biol 2006 : Pretreatment with quercetin and the PI 3-kinase inhibitor LY294002 each reduced TNF-alpha induced IL-8 and monocyte chemoattractant protein (MCP)-1 ( also called CCL2 ) expression in cultured human airway epithelial cells
Teferedegne et al., Mol Cell Biol 2006 : The monocyte chemoattractant protein 1 gene ( MCP-1 ) is regulated by TNF through an NF-kappaB dependent distal enhancer and an Sp1 dependent promoter-proximal regulatory region
Escobar et al., Surgery 2006 : TNF-alpha induced release of monocyte chemoattractant protein 1 ( MCP-1 ) was determined by enzyme linked immunosorbent assay ... CHCsil decreased TNF-alpha induced MCP-1 by 46 % ( P < .05 ), compared with control
Fujii et al., Arterioscler Thromb Vasc Biol 2006 (Ischemia) : Tumor necrosis factor (TNF)-alpha stimulated MCP-1 expression in all cell types tested, whereas FGF-2 mediated upregulation of MCP-1 was found only in NEMCs but not in others, a finding that was not affected by VEGF in vitro and in vivo
Kim et al., Antioxid Redox Signal 2007 : Effects of PGC-1alpha on TNF-alpha induced MCP-1 and VCAM-1 expression and NF-kappaB activation in human aortic smooth muscle and endothelial cells ... Consequently, NF-kappaB activity and MCP-1 and VCAM-1 induced by TNF-alpha are suppressed
Qin et al., Glia 2007 (Disease Models, Animal...) : Systemic TNFalpha and LPS administration activated microglia and increased expression of brain pro-inflammatory factors ( i.e., TNFalpha, MCP-1 , IL-1beta, and NF-kappaB p65 ) in wild-type mice, but not in TNF R1/R2 ( -/- ) mice
Li et al., Nephrol Dial Transplant 2007 (Anoxia...) : PPAR-gamma 's activation with 15d-PGJ2 or pioglitazone reduced basal and TNF-alpha stimulated MCP-1 expression at mRNA and protein levels at 24 h under normoxia
Matsui et al., J Int Med Res 2006 : Azelnidipine, 10 nmol/l, was found to inhibit the TNF-alpha induced upregulation of monocyte chemoattractant protein-1 mRNA levels in human umbilical vein endothelial cells significantly ... Furthermore, azelnidipine suppressed TNF-alpha induced monocyte chemoattractant protein-1 production by human umbilical vein endothelial cells
Hirano et al., Eur J Pharmacol 2007 : Synergistic effect of PGD2 via prostanoid DP receptor on TNF-alpha induced production of MCP-1 and IL-8 in human monocytic THP-1 cells ... In addition, the selective prostanoid DP receptor antagonist, pinagladin ( ( Z ) -7- [ ( 1R,2R,3S,5S ) -2- ( benzothiophen-3-ylcarbonylamide ) -10-norpinan-3-yl ] hept-5-enoic acid ) inhibited the production of MCP-1 and IL-8 upon combined stimulation with PGD2 and TNF-alpha ... Our findings suggest that activation of the prostanoid DP receptor on THP-1 cells enhances TNF-alpha induced MCP-1 and IL-8 production via the cAMP/PKA signaling pathway
Knaapen et al., Mutat Res 2007 (Atherosclerosis) : In addition to its effect on basal gene expression, we showed that B [ a ] P significantly enhanced TNFalpha induced expression of MCP-1
Sharma et al., Am J Physiol Lung Cell Mol Physiol 2007 (Inflammation...) : H/R ( 3 h/1 h ) significantly induced KC, MCP-1 , macrophage inflammatory protein-2 ( MIP-2 ), RANTES, and IL-6 ( but not TNF-alpha ) by MLE-12 cells, whereas H/R induced TNF-alpha , MCP-1, RANTES, MIP-1alpha, and MIP-2 ( but not KC ) by RAW264.7 cells ... Importantly, using macrophage and epithelial coculture methods, the specific production of TNF-alpha by H/R exposed RAW264.7 cells significantly induced proinflammatory cytokine/chemokine expression ( KC, MCP-1 , MIP-2, RANTES, and IL-6 ) by MLE-12 cells
Schreml et al., Int Immunopharmacol 2007 : mTOR-inhibitors failed to suppress basal/TNF induced secretion of IL-8 and MCP-1 , but IL-6 synthesis after TNF-induction was reduced to 35 %
Takano et al., Biochem Biophys Res Commun 2007 : We found that, in glomerular podocytes, induction of monocyte chemoattractant protein 1 ( MCP-1 ) and inducible nitric oxide synthase (iNOS) by TNF-alpha was abrogated by K-7174 ... Furthermore, K-7174 elicited UPR abrogated induction of MCP-1 and iNOS not only by TNF-alpha but also by medium conditioned by activated macrophages
Elizur et al., Am J Respir Cell Mol Biol 2008 : Using a transformed murine Clara cell line ( C22 ), we observed that both LPS and TNF-alpha induced production of keratinocyte derived chemokine ( KC ) and monocyte chemoattractant protein (MCP)-1
Boero et al., Immunol Lett 2007 : The presence of ciclesonide in cell cultures induced a significant downregulation of : ( a ) bFGF induced fibroblast proliferation and TNF-alpha induced ICAM-1 expression, at the 0.3-9.0 microM concentrations ( p < 0.05 ) ; ( b ) TNF-alpha induced MCP-1 release, at all the concentrations tested ( p < 0.05 ) ; ( c ) TNF-alpha induced eotaxin release, at the three highest concentrations ( 0.9-9.0 microM ) ( p < 0.05 ) ; ( d ) TGF-beta1 induced of alpha-SMA protein expression at the 0.3-3.0 microM concentrations, associated with a reduction in the organization of alpha-SMA stress fibres
Son et al., Cancer Biol Ther 2007 (Carcinoma...) : ID8 cells constitutively expressed CXCL16 and CCL2 , but only CCL2 expression was enhanced by LPS, IL-1 and TNF
Kang et al., Biol Pharm Bull 2007 : TNF-alpha induced monocyte chemoattractant protein 1 ( MCP-1 ) expression was also attenuated by treatment of cornuside
Sun et al., Am J Physiol Gastrointest Liver Physiol 2007 (Pancreatitis) : Prostaglandin E2 modulates TNF-alpha induced MCP-1 synthesis in pancreatic acinar cells in a PKA dependent manner ... In the presence of TNF-alpha , MCP-1 was upregulated ... Coincubation with PGE ( 2 ) enhanced the TNF-alpha induced MCP-1 synthesis significantly ... PGE ( 2 ) modulates the TNF-alpha induced MCP-1 synthesis and secretion from acinar cells
Cho et al., Exp Mol Med 2007 (Arthritis, Rheumatoid) : MCP-1 increased the proliferation of FLS and the production of IL-15, TNF-alpha , and IL-18
Conover et al., Growth Horm IGF Res 2008 : TNF-alpha induced VCAM, ICAM, and MCP-1 expression ( 4h ) preceded PAPP-A expression ( 24h )
Guo et al., Free Radic Biol Med 2008 : CH3-Avn-c, a synthetically prepared methyl ester derivative of Avn-c with a high biological potency, significantly and dose dependently decreased mRNA expression and secretion of IL-6, IL-8, and MCP-1 by HAEC as determined by real-time RT-PCR and ELISA, and it inhibited IL-1beta- and TNFalpha stimulated NF-kappaB activation as determined by a NF-kappaB DNA binding assay and a NF-kappaB luciferase reporter assay
Matsui et al., J Int Med Res 2007 : We have previously shown that nifedipine, one of the most popular DHPs, blocks tumour necrosis factor-alpha (TNF-alpha) induced monocyte chemoattractant protein-1 as well as vascular cell adhesion molecule-1 ( VCAM-1 ) expression in endothelial cells by suppressing reactive oxygen species generation ( ROS )
Lin et al., Arterioscler Thromb Vasc Biol 2008 (Inflammation) : FIR radiation also inhibited tumor necrosis factor (TNF)-alpha mediated expression of E-selectin, vascular cell adhesion molecule-1, intercellular cell adhesion molecule-1, monocyte chemoattractant protein-1 , interleukin-8, and the cytokine mediated adhesion of monocytes to ECs
Zhu et al., Biochem Biophys Res Commun 2008 (Inflammation) : Anti-inflammatory effect of resveratrol on TNF-alpha induced MCP-1 expression in adipocytes ... In this study we investigated the effects of resveratrol upon both tumor necrosis factor (TNF)-alpha induced MCP-1 gene expression and its underlying signaling pathways in 3T3-L1 adipocytes ... Resveratrol was found to inhibit TNF-alpha induced MCP-1 secretion and gene transcription, as well as promoter activity, which based on down-regulation of TNF-alpha induced MCP-1 transcription ... Nuclear factor (NF)-kappaB was determined to play a major role in the TNF-alpha induced MCP-1 expression
Ahn et al., Life Sci 2008 : Epigallocatechin-3-O-gallate inhibits TNFalpha induced monocyte chemotactic protein-1 production from vascular endothelial cells ... In this study, we investigated the mechanisms by which EGCG may inhibit tumor necrosis factor-alpha (TNFalpha) induced MCP-1 production in bovine coronary artery endothelial cells ... TNFalpha increased MCP-1 production in both a concentration and time dependent manner ... Inhibitors of phosphatidylinositol-3-OH kinase ( PI-3 kinase ), LY294002 and wortmannin, decreased TNFalpha induced MCP-1 production ... EGCG prevented TNFalpha mediated MCP-1 production and reduced phosphorylation of Akt ( Ser473 ) ... In conclusion, EGCG inhibited TNFalpha induced MCP-1 production
Takahashi et al., Am J Physiol Heart Circ Physiol 2008 (Disease Models, Animal...) : Furthermore, ANG II- and TNF-alpha stimulated MCP-1 expression was partially suppressed by ROS inhibitors ... These results suggested that ANG II and TNF-alpha synergistically stimulate MCP-1 expression via the utilization of distinct intracellular signaling pathways ( p38- and NFkappaB dependent pathways ) and that these pathways are activated in ROS dependent and -independent manners
Liebler et al., Mediators Inflamm 1994 : Simultaneous stimulation with IFN-gamma and IL-1beta or TNF-alpha resulted in no further increase in MCP-1 production
Goldstein et al., Mediators Inflamm 1996 : TNF induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1
Winterbone et al., Atherosclerosis 2009 : Quercetin was able to reduce TNFalpha induced upregulation of VCAM-1, ICAM-1 and MCP-1 at both the protein and transcript ( mRNA ) level in HUASMC
Funakoshi-Tago et al., Biochem Pharmacol 2008 : As a result, TNFalpha induced expression of inflammatory cytokines, CXCL1/KC and CCL2/MCP-1 , was clearly inhibited by Celecoxib
Ho et al., Immunobiology 2008 (Inflammation...) : TNF-alpha could significantly induce CCL2 , ICAM-1 and VCAM-1 expression of PTEC ... Selective inhibitors of p38 MAPK ( SB203580 ), JNK ( SP600125 ) and ERK ( PD98059 ) could suppress TNF-alpha induced CCL2 and ICAM-1 expression, while only p38 MAPK and ERK inhibitors could suppress TNF-alpha induced VCAM-1 expression
Zehnder et al., Kidney Int 2008 (Chronic Disease...) : In vitro, 1,25 ( OH ) 2D attenuated TNFalpha induced MCP-1 expression by human proximal tubule cells
Bian et al., Invest Ophthalmol Vis Sci 2008 : Effects of tunicamycin and thapsigargin on IL-1beta- and TNF-alpha stimulated MCP-1 mRNA expression and protein production were further examined by RT-PCR and ELISA, respectively ... They also caused corresponding reductions in IL-1beta- and TNF-alpha induced MCP-1 mRNA expression and protein production
Okamura et al., American journal of physiology. Renal physiology 2008 : We found that, in murine podocytes, expression of monocyte chemoattractant protein 1 ( MCP-1 ) in response to TNF-alpha was suppressed by indomethacin but not by ibuprofen
Machado et al., Microbes Infect 2008 : Interestingly, T. cruzi infected NOS2-deficient CMs produced significantly higher levels of CCL2 , CCL4, CCL5 and CXL2 in the presence of IFN-gamma and TNF
Ju et al., Int J Mol Med 2008 : The results demonstrated that glucosamine but not N-acetylglucosamine suppressed TNF-alpha induced expression of MCP-1 and ICAM-1 at both the mRNA and protein levels ... Thus, glucosamine is likely to suppress endothelial cell activation ( TNF-alpha induced ICAM-1 and MCP-1 expression ) possibly by affecting p38MAPK and NF-kappaB signaling via O-GlcNAc modification
Ahmed et al., Endocrine 2009 : c-Jun N-terminal kinases inhibitor suppresses the TNF-alpha induced MCP-1 expression in human umbilical vein endothelial cells ... A recent report indicated that MCP-1 expression in human umbilical vein endothelial cells ( HUVECs ) is induced by the stimulation of tumor necrosis factor (TNF)-alpha via the c-Jun N-terminal kinases (JNK) pathway ... As expected, JNKI-1 blocked the stimulatory effect of TNF-alpha on the MCP-1 promoter activity ... In conclusion, JNKI-1 partially inhibits the TNF-alpha induced MCP-1 expression in HUVECs, and therefore JNKI-1 may be of therapeutic value in the treatment of diseases such as atherosclerosis
Engberding et al., Arterioscler Thromb Vasc Biol 2009 (Diabetes Mellitus, Type 2...) : IGF1R downregulation uncovered an insulin induced reduction in activation of NF-kappaB and inhibition of MCP-1 upregulation in response to TNF-alpha
Hayakawa et al., J Immunol 2009 : Priming of the cells with ER stress inducers ( tunicamycin, thapsigargin, A23187, and AB5 subtilase cytotoxin ) caused blunted induction of MCP-1 in response to TNF-alpha , IL-1beta, macrophage derived factors, or bystander macrophages
Subileau et al., J Neuropathol Exp Neurol 2009 (Inflammation...) : Tumor necrosis factor strongly increased the production of CCL2 , CCL5, and CXCL8 ; interferon gamma upregulated CXCL10 exclusively
Zhang et al., Invest Ophthalmol Vis Sci 2009 (Diabetes Mellitus, Experimental...) : Studies using human ECs demonstrated that TNF-alpha induced CCL2 production was also inhibited by the NAD ( P ) H oxidase inhibitor DPI, the antioxidant N-acetyl-L-cysteine, or the superoxide scavenger Tiron, further indicating that inhibition occurs through the NAD ( P ) H/ROS pathway
Chen et al., Inflamm Res 2009 : Sulforaphane inhibits TNF-alpha induced activation of p38 MAP kinase and VCAM-1 and MCP-1 expression in endothelial cells ... One-hour pretreatment of endothelial cells ( EC ) with sulforaphane ( 1-4 muM ) suppressed TNF-alpha induced MCP-1 and VCAM-1 mRNA and protein levels, but had no effect on TNF-alpha induced ICAM-1 expression
Gao et al., J Neurosci 2009 (Neuralgia...) : MCP-1 upregulation by TNF-alpha was dose dependently inhibited by the JNK inhibitors SP600125 ( anthra [ 1,9-cd ] pyrazol-6 ( 2H ) -one ) and D-JNKI-1
Kasahara et al., Immunology 1991 (Astrocytoma) : These data suggest that IL-1 and TNF-alpha induce astrocytes to produce IL-8 and MCAF transcriptionally and post-transcriptionally, both of which may be responsible for leucocytosis seen in inflammation of the CNS
Lin et al., J Endod 2009 (Alveolar Bone Loss...) : The study assessed the effect of simvastatin on tumor necrosis factor alpha (TNF- alpha) induced synthesis of Cyr61 and CCL2 in MG-63 human osteoblastic cells ... Simvastatin also reduced the levels of TNF-alpha induced CCL2 , and exogenous Cyr61 restored the inhibitory effects
Murao et al., Atherosclerosis 2009 : MCP-1 is expressed in HUVECs in response to several different stimuli, including interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha ... MCP-1 expression was regulated by IL-1beta and TNF-alpha and cytokine induced PREB expression
Ding et al., Biomed Mater 2009 (Inflammation) : Furthermore, these data suggested that MCP-1 was regulated by TNF-alpha and IL-1beta, and activated by both cytokines and biomaterials
Nichols et al., J Immunol 2009 (Brain Abscess) : Despite receptor expression, IL-17 was relatively ineffective at eliciting glial activation, whereas the cytokine augmented the ability of TNF-alpha to induce CXCL2 and CCL2 expression by macrophages
Du et al., J Immunol 2009 : Furthermore, down-regulation of C/EBPbeta by small interfering RNA substantially reversed the suppressive effect of CsA on TNF-alpha induced MCP-1 expression
Kageyama et al., Mod Rheumatol 2009 (Arthritis, Rheumatoid) : TNF-alpha induced MCP-1 , RANTES, and granzyme B production in cultured synovial cells from RA patients
Ikeda et al., IUBMB Life 2009 : TNFalpha , but not TPA, increased IL-6 and MCP-1 mRNA levels, Next, we investigated the effects of ligands which activate c/nPKC
Thompson et al., Brain Res 2009 (MAP Kinase Signaling System) : This study aims to test the ability of IL-1beta and TNF-alpha to stimulate CCL2 and CCL7 protein production in rat astrocyte cultures, and to elucidate signaling pathways involved in the cytokine stimulated chemokine upregulation
Yang et al., Am J Physiol Heart Circ Physiol 2009 (Diabetes Mellitus, Type 2...) : Tumor necrosis factor-alpha (TNF-alpha) upregulates the expression of monocyte chemoattractant protein-1 ( MCP-1 ) and adhesion molecules in type 2 diabetes
Ruan et al., Curr Alzheimer Res 2009 (Alzheimer Disease...) : Expression of TNF-alpha was detected at 8 months of age, while IL-1 beta, IL-6 and MCP-1 at 10 months
Kim et al., Eur J Pharmacol 2009 (Inflammatory Bowel Diseases) : TNF-alpha induced mRNA induction of the chemokines, monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8, and the intercellular cell adhesion molecule ( ICAM)-1, which are involved in adhesion between leukocytes and epithelial cells, was suppressed by M1-M4, and M1 was the most efficacious
Fu et al., J Vet Med Sci 2009 (Inflammation) : Furthermore, vaspin did not decrease the TNF-alpha ( 24 hr ) induction of vascular cell adhesion molecule-1, intercellular adhesion molecule-1, endothelial selectin, and cyclooxygenase-2 protein expression as well as monocyte chemotactic protein-1 , tissue factor, and plasmogen activator inhibitor-1 mRNA expression
Shen et al., J Biol Chem 2009 (Inflammation...) : We showed that kallistatin inhibits tumor necrosis factor-alpha induced NF-kappaB activation, as well as vascular cell adhesion molecule-1 and monocyte chemoattractant protein-1 expression in endothelial cells, whereas knockdown of KLF4 by small interfering RNA oligonucleotide abolished the effect of kallistatin
Létuvé et al., Am J Pathol 2010 (Asthma...) : Tumor necrosis factor-alpha stimulated ChTRase release only from alveolar macrophages from smokers with COPD, and exposure of these cells to ChTRase promoted the release of IL-8, monocyte-chemoattractant protein-1 , and metalloproteinase-9
Hu et al., J Biomed Biotechnol 2009 : In this study, we found that ticlopidine dose-dependently decreased the mRNA and protein levels of TNF-alpha stimulated MCP-1 , IL-8, and vascular cell adhesion molecule-1 ( VCAM-1 ) in human umbilical vein endothelial cells ( HUVECs )
Funakoshi-Tago et al., Int Immunopharmacol 2010 (Edema) : In addition, TNFalpha induced expression of inflammatory cytokines, CCL2/MCP-1 and CXCL1/KC, was clearly inhibited by Licochalcone A but not reduced Licochalcone A
Kim et al., Atherosclerosis 2010 (Inflammation) : We found that 5'-NIO inhibited TNF-alpha induced MCP-1 and IL-8 expression at the RNA and protein levels in HUVECs
Standiford et al., J Biol Chem 1991 : These data are consistent with the induction of MCP-1 expression from A549 cells by TNF and IL-1
Giraud et al., Proc Natl Acad Sci U S A 2010 (Encephalomyelitis, Autoimmune, Experimental) : In cultured rodent astrocytes, E2 or an ERalpha agonist, but not an ERbeta agonist, inhibits TNFalpha induced CCL2 expression at nanomolar concentrations, and the ER antagonist ICI 182,170 blocks this effect
Hacke et al., Molecular cancer 2010 (Cell Transformation, Neoplastic) : Moreover, the availability of p53 is apparently important for chemokine regulation, since TNF-alpha can induce MCP-1 only in human keratinocytes expressing the viral oncoprotein E7, but not in HPV16 E6 positive cells, where p53 becomes degraded
Altan et al., J Pharmacol Exp Ther 2010 (Endometriosis) : TBP and TNF-SHARC dose-dependently inhibited TNFalpha induced secretion of interleukin (IL)-6, IL-8, granulocyte macrophage-colony stimulating factor, and monocyte chemoattractant protein-1 in immortalized human endometriotic cells
Ryan et al., Pflugers Arch 2010 : IL-6 release into the medium was increased > 50-fold over 24 h with LPS and TNFalpha, while MCP-1 release was increased 23- and 40-fold by TNFalpha and LPS, respectively
Hosokawa et al., Arch Biochem Biophys 2010 (Diabetes Complications...) : In differentiating 3T3-F442A adipocytes, fucoxanthinol, which is a fucoxanthin metabolite found in WAT, attenuated TNF-a induced MCP-1 and IL-6 mRNA overexpression and protein secretion into the culture medium
Wang et al., Int J Food Microbiol 2010 : Effect of live Lactobacillus plantarum L2 on TNF-alpha induced MCP-1 production in Caco-2 cells
Hattori et al., Diabetologia 2010 : It also reduced TNFalpha induced MCP-1 ( also known as CCL2 ), VCAM1, ICAM1 and E-selectin mRNA expression
Mueller et al., Biochem Biophys Res Commun 2010 (Colonic Neoplasms...) : TNF-alpha similarly induces IL-6 and MCP-1 in fibroblasts from colorectal liver metastases and normal liver fibroblasts
Diaz Encarnacion et al., American journal of physiology. Renal physiology 2011 : DHA and EPA decreased TNF-a stimulated MCP-1 mRNA expression by decreasing transcription of the MCP-1 gene ... We conclude that DHA and EPA inhibit TNF-a stimulated transcription of the MCP-1 gene through interaction of signaling pathways involving ERK and NF-?B
Lee et al., Exp Neurol 2011 : Extracellular HIV-1 Tat upregulates TNF-a dependent MCP-1/CCL2 production via activation of ERK1/2 pathway in rat hippocampal slice cultures : inhibition by resveratrol, a polyphenolic phytostilbene
Yoshizato et al., Int J Oncol 1996 : We compared the in vitro effects of various cytokines on the expression of monocyte chemoattractant protein-1 ( MCP-1 ) in glioma cell lines and found that MCP-1 expression was highly induced by tumor necrosis factor-alpha (TNF alpha) and interleukin-1 beta
Sica et al., J Immunol 1990 : TNF and LPS, unlike IL-6, also induced MCAF/MCP-1/TDCF gene expression
Honda et al., Anticancer Res 2011 (Colonic Neoplasms...) : RESULTS : In THP-1 cells, monocyte chemotactic protein (MCP)-1 mRNA expression increased in a time dependent manner from day 3 after co-culture with DLD-1 cells ; furthermore, expression of vascular endothelial growth factor ( VEGF ) -A, tumor necrosis factor (TNF)-a , interleukin (IL)-1ß, and IL-8 mRNA was increased from day 5
Bian et al., Invest Ophthalmol Vis Sci 2011 : Caspase-5 knockdown reduced caspase-1 protein expression and activation and inhibited TNF-a induced IL-8 and MCP-1
McClellan et al., Cytokine 2012 (Adenomatous Polyposis Coli...) : Gene expression ( mRNA ) of MCP-1, IL-1ß, IL-6 and TNF-a was increased in sections 2 and 3 starting at week 12 ( P < 0.05 ), with further increases in MCP-1 , IL-1ß and IL-6 at 16 weeks ( P < 0.05 )
Matsunaga et al., Mol Med Report 2012 : ?-Tocotrienol attenuates TNF-a induced changes in secretion and gene expression of MCP-1 , IL-6 and adiponectin in 3T3-L1 adipocytes ... ?-tocotrienol effectively improved the TNF-a induced adverse changes in MCP-1, IL-6 and adiponectin secretion, and in MCP-1 , IL-6, adiponectin and PPAR? mRNA expression
Kular et al., Journal of neuroinflammation 2012 (Disease Models, Animal...) : In vitro, results based on cultured DRG neurons showed that siRNA mediated inhibition of NOV enhanced IL-1ß- and TNF-a induced MMP-2, MMP-9 and CCL2 expression whereas NOV addition inhibited TNF-a induced MMP-9 expression through ß1 integrin engagement
Antonelli et al., J Endocrinol 2012 (Graves Ophthalmopathy) : We have tested the effect of interferon ? ( IFN? ( IFNG ) ) and tumor necrosis factor a (TNFa) on CCL2 , and for comparison on the prototype a chemokine (C-X-C motif) ligand 10 ( CXCL10 ), and the possible modulatory role of PPARa activation on secretion of these chemokines in normal and GO fibroblasts or preadipocytes in primary cell cultures
Chuquimia et al., PloS one 2012 : Interestingly, TNF could stimulate the production of CCL2/MCP-1
Kim et al., J Nutr Biochem 2012 (Myocardial Infarction...) : In CMs, TLR2 and monocyte chemoattractant protein (MCP)-1 mRNAs were increased by TNF-a , PGN or H/R, whereas they were blunted by curcumin
Wang et al., Invest Ophthalmol Vis Sci 2012 : TNF-a significantly increased the expression of IL-6, IL-8, and MCP-1 in ARPE-19 cells at both the protein and mRNA levels
Gambhir et al., Invest Ophthalmol Vis Sci 2012 (Diabetes Mellitus, Experimental...) : TNF-a increased the expression and secretion of IL-6 and Ccl2 in ARPE-19 cells ... In contrast, GPR109A ligands failed to suppress TNF-a induced expression and secretion of IL-6 and Ccl2 in primary RPE cells from Gpr109a ( -/- ) mice, confirming that the observed anti-inflammatory effects were mediated specifically by Gpr109a
Siriwardhana et al., J Nutr Biochem 2012 (Inflammation) : Moreover, EPA attenuated tumor necrosis factor-a induced MCP-1 and further reduced its secretion in the presence of an NF-?B inhibitor
Ma et al., J Pharmacol Sci 2012 : We also demonstrated that pretreatment of cells with this compound prevented TNF-a induced expression of NF-?B target genes, such as interleukin 6, interleukin 8, monocyte chemotactic protein 1 , cyclooxygenase-2 and inducible nitric oxide
Hou et al., Wei Sheng Yan Jiu 2012 : [ Effects of citreoviridin on the expression of MCP-1 and ILs induced by TNF-alpha in vein endothelial cells ] ... To investigate the effects of citreoviridin (CIT) on the expression of MCP-1 , IL-1beta, IL-6 and IL-8 induced by TNF-alpha in human umbilical vein endothelial cells ( HUVECs )
Lobo et al., Cytokine 2012 : Our findings suggested that simvastatin counteracted the stimulatory effect of TNF-a on secretion and expression of MCP-1 , PAI-1 and adiponectin, implying a potential anti-atherogenic effect during the inflammatory process ; these pleitropic effects were more pronounced with HMG-CoA reductase inhibitor
Mehta et al., Journal of translational medicine 2012 (Inflammation...) : Adipose tissue mRNA levels of IL-6 ( peak 6-fold, ANOVA F = 27.5, p < 0.001 ) and TNF-alpha ( peak 1.8-fold, F = 2.9, p = 0.01 ) increased with MCP-1 ( peak 10-fold, F = 5.6, p < 0.01 ) and fractalkine ( CX3CL1 ) ( peak 15-fold, F = 13.3, p < 0.001 )
Tsou et al., J Cell Biochem 2012 (Osteoarthritis) : Apoptosis signal regulating kinase 1 is mediated in TNF-a induced CCL2 expression in human synovial fibroblasts ... Here, we investigated the intracellular signaling pathways involved in TNF-a induced monocyte chemoattractant protein 1 ( MCP-1 ) /CCL2 expression in human synovial fibroblast cells ... Stimulation of synovial fibroblasts ( OASF ) with TNF-a induced concentration- and time dependent increases in CCL2 expression ... TNF-a mediated CCL2 production was attenuated by TNFR1 monoclonal antibody ( Ab ) ... Our results suggest that the interaction between TNF-a and TNFR1 increases CCL2 expression in human synovial fibroblasts via the ASK1, JNK/p38, c-Jun, and AP-1 signaling pathway
Lee et al., International journal of molecular sciences 2012 : AP also effectively reduced TNF-a induced mRNA expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 in a dose dependent manner
Watanabe et al., Br J Anaesth 2013 (Anoxia...) : /st > Sevoflurane suppressed TNF-a induced IL-6, IL-8, and MCP-1 gene expression and the production of IL-6 and IL-8 in SAEC under anoxia/reoxygenation conditions
Koo et al., Cell Biol Int 2013 : We have investigated whether MPA regulates Syk to repress tumour necrosis factor-a (TNF-a) induced MCP-1 production in cultured human aortic endothelial cells ... TNF-a increased MCP-1 at both mRNA and protein levels ... TNF-a induced MCP-1 mRNA expression was inhibited by N-acetylcysteine (NAC), Syk inhibitor, Syk-siRNA and MPA ... TNF-a induced MCP-1 protein production was also inhibited by Syk inhibitor and MPA ... TNF-a increased DNA binding activity of AP-1 and NF-?B, whereas both AP-1 and NF-?B decoy oligodeoxynucleotides downregulated TNF-a induced MCP-1 mRNA expression ... TNF-a induced MCP-1 expression via activation of AP-1 and NF-?B
Bocian et al., J Biol Chem 2013 (Disease Models, Animal...) : Upon incubation with decorin, endothelial cells and fibroblasts responded differently to IFN-? and TNF-a ; CCL2 in bEnd.3 cells was more prominently up-regulated by TNF-a compared with IFN-?
Sun et al., Inflamm Res 2013 (Dermatitis, Atopic) : Tumor necrosis factor-a , monocyte chemoattractant protein-1 and intercellular adhesion molecule-1 increase during the development of a 2,4-dinitrofluorobenzene induced immediate-type dermatitis in rats
Liu et al., BioMed research international 2013 (Hypertension, Pulmonary...) : GW501516 also significantly attenuated TNF mediated expression of MCP-1
Lin et al., J Endod 2013 : Simvastatin and FoxO3a diminished TNF-a induced CCL2 secretion and macrophage recruitment, whereas Cyr61 partially restored the stimulating action
Jiang et al., Nan Fang Yi Ke Da Xue Xue Bao 2013 : After 48h incubation, the expression of MCP-1 and FN increased significantly in TNF-a group ( P < 0.01 ), which was lowered by exendin-4 in TNF-a+E1,TNF-a+E5 and TNF-a+E10 groups ( P < 0.05 ) ... Exendin-4 has an intrinsic capability to concentration- and time-dependently inhibit TNF-a induced expression of MCP-1 and FN in rat mesangial cells, suggesting the beneficial effect of exendin-4 in preventing and treating diabetic nephropathy
Kong et al., Lipids in health and disease 2013 : However, comparing to the model rabbits, levels of TNF-a , IL-1ß and MDA decreased significantly and serum SOD activity increased , gene and protein expressions of E-sel, ICAM-1, VCAM-1 and MCP-1 in aortas decreased significantly with the treatment of kaempferol
Hayashi et al., J Neuroimmunol 1995 : Lipopolysaccharide (LPS), interleukin (IL)-1 beta and tumor necrosis factor (TNF)-alpha induced MCP-1 secretion by astrocytes, but not microglia
Brieland et al., Am J Respir Cell Mol Biol 1995 : We determined that stimulation of resident rat PAMs with bacterial lipopolysaccharide (LPS), murine tumor necrosis factor-alpha , or human interleukin-1 beta resulted in the inducible expression of MCP-1 mRNA and the secretion of biologically active MCP-1
Flory et al., Am J Pathol 1995 (Granuloma...) : Because tumor necrosis factor-alpha (TNF-alpha) and interleukin 1 (IL-1) can induce MCP-1 production in a variety of cell types, we sought to determine their potential regulatory roles in this model
Li et al., Mol Cell Biochem 1994 (Glioblastoma) : This result suggested that the TNF regulation of MCP-1 gene involves other parts of the gene besides the proximal 5 ' flanking region
Zhu et al., J Bone Miner Res 1994 : The effects of interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha) , interleukin-6 (IL-6), transforming growth factor beta ( TGF-beta ), platelet derived growth factor ( PDGF-BB ), parathyroid hormone (PTH), and 1,25 ( OH ) 2D3 on MCP-1 expression in human osteoblastic cells were compared ... MCP-1 expression at both the protein and mRNA levels was greatly increased by IL-1 beta and TNF-alpha ... At the mRNA level, IL-1 beta and TNF-alpha strongly induced MCP-1 expression ; TGF-beta and IL-6 induced MCP-1 but to a lesser extent
Leonard et al., Adv Exp Med Biol 1993 : TNF alpha did not cause secretion of MCP-1 , but caused about the same amount of NAP-1 secretion as IL-2 + IFN gamma
Hanazawa et al., J Biol Chem 1994 : Retinoic acid suppression of c-fos gene inhibits expression of tumor necrosis factor-alpha induced monocyte chemoattractant JE/MCP-1 in clonal osteoblastic MC3T3-E1 cells ... Our previous study ( Hanazawa, S., Takeshita, A., Amano, S., Semba, T., Nirazuka, T., Katoh, H., and Kitano, S. ( 1993 ) J. Biol. Chem. 268, 9526-9532 ) demonstrated that tumor necrosis factor-alpha (TNF-alpha) induces monocyte chemoattractant JE/MCP-1 expression via c-fos and c-jun genes following protein kinase C activation in osteoblastic MC3T3-E1 cells
Colotta et al., Am J Pathol 1994 : Induction of MCP-1 transcript by thrombin was not reduced by blocking interleukin-1 and tumor necrosis factor , suggesting that these mediators are not involved in thrombin induced expression of MCP-1
Grandaliano et al., J Lab Clin Med 1994 : Gamma interferon ( IFN-gamma ), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 (IL-1) markedly stimulate the release of MCP-1 as measured by a specific and sensitive radioimmunoassay
Satriano et al., J Immunol 1993 : Regulation of monocyte chemoattractant protein-1 and macrophage colony stimulating factor-1 by IFN-gamma, tumor necrosis factor-alpha , IgG aggregates, and cAMP in mouse mesangial cells
Marra et al., J Clin Invest 1993 (Inflammation) : Tumor necrosis factor-alpha (TNF alpha) also increased MCP-1 secretion, although to a lesser extent ( 1.6-fold )
Hachicha et al., Arthritis Rheum 1993 : Use of cycloheximide and actinomycin D confirmed that TNF alpha was inducing MCP-1 expression at both the transcriptional and translational levels ... These studies demonstrate that the MCP-1 gene is regulated by TNF alpha and IFN gamma in type B synoviocytes and indicate that these cells may play an important role in the recruitment of inflammatory cells to the rheumatoid synovial environment, via the production of novel chemotactic cytokines such as MCP-1
Paine et al., J Immunol 1993 : Northern blot analysis revealed that IL-1 and TNF-alpha stimulated MCP-1 mRNA expression in a dose dependent manner, whereas dexamethasone blocked MCP-1 expression by cells stimulated with IL-1
Akahoshi et al., Arthritis Rheum 1993 (Arthritis, Rheumatoid) : Fibroblast-like synoviocytes were found to express MCAF mRNA and to secrete MCAF in response to interleukin-1 (IL-1) and tumor necrosis factor in vitro
Kakizaki et al., Kidney Int 1995 (Glomerulonephritis) : Northern blot analysis revealed that MCP-1 mRNA was constitutively expressed by GEN and that IL-1 beta and tumor necrosis factor-alpha (TNF-alpha) increased MCP-1 mRNA levels in a dose- and time dependent manner ... Inhibition study using protein kinase inhibitors revealed that MCP-1 mRNA expression induced by IL-1 beta and TNF-alpha was suppressed by the tyrosine kinase inhibitor genistein, not by the protein kinase C inhibitors staurosporine or H-7, or the protein kinase A inhibitor H-89, suggesting an important role of tyrosine kinase in the cytokine induced MCP-1 gene expression
Meda et al., J Immunol 1996 : Finally, we provide evidence that the Abeta ( 25-35 ) - and Abeta plus IFN-gamma induced production of MCP-1 is, in large part, mediated in an autocrine fashion by endogenous TNF-alpha
Pai et al., J Am Soc Nephrol 1996 : Role of tumor necrosis factor-alpha on mesangial cell MCP-1 expression and monocyte migration : mechanisms mediated by signal transduction ... This study examined the role of tumor necrosis factor (TNF)-alpha mediated cellular signal transduction pathways on mesangial cell MCP-1 gene expression and monocyte migration ... Similarly, calphostin C, a PKC inhibitor, failed to inhibit TNF-alpha induced MCP-1 expression ... However, the coincubation of mesangial cells with TNF-alpha and dbcAMP completely inhibited TNF-alpha induced MCP-1 gene expression ... TNF-alpha induced mesangial cell MCP-1 expression is regulated by signal transduction pathways involving PTK but not those dependent on PKC or cAMP
Sato et al., J Rheumatol 1996 (Arthritis, Rheumatoid) : Antioxidants inhibit tumor necrosis factor-alpha mediated stimulation of interleukin-8, monocyte chemoattractant protein-1 , and collagenase expression in cultured human synovial cells ... TNF-alpha increased the expression of IL-8, MCP-1 , and collagenase mRNA in human synovial cells ... Our data suggest that TNF-alpha induces expression of proinflammatory cytokines such as IL-8 and MCP-1 through generation of reactive oxygen intermediates and subsequent activation of NF-kappa B in human synovial cells, and the antioxidants may inhibit, at least in part, the activation of NF-kappa B by TNF-alpha
Jordan et al., Br J Pharmacol 1996 : Ro 31-8220 also inhibited the expression of IL-1 alpha or TNF alpha induced MCP-1 and RANTES mRNA with a similar potency
Bodine et al., Endocrinology 1996 : Finally, interleukin-1 beta and tumor necrosis factor-alpha ( 1-1000 pM ) stimulated dose dependent increases in the secretion of interleukin-6 and monocyte chemoattractant protein-1 at 34 C and 40C
Ozaki et al., Oral Microbiol Immunol 1996 (Periodontitis) : Interleukin-1 beta and tumor necrosis factor-alpha stimulate synergistically the expression of monocyte chemoattractant protein-1 in fibroblastic cells derived from human periodontal ligament ... The present study demonstrates that interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) induce and synergistically stimulate monocyte chemoattractant protein-1 ( MCP-1 ) expression in fibroblasts from human periodontal ligament ... IL-1 beta and TNF-alpha induced in a dose dependent manner the expression of the MCP-1 gene in the fibroblasts from the human periodontal ligament
Elner et al., Exp Eye Res 1996 : In this study, we examined the ability of interleukin-7 to induce RPE derived monocyte chemotactic protein-1 and interleukin-8 and assessed the potentiating effects of interleukin-7 on interleukin-1 beta- and tumor necrosis factor-alpha induced RPE monocyte chemotactic protein-1 and interleukin-8 production ... Interleukin-7 potentiated tumor necrosis factor-alpha induced RPE monocyte chemotactic protein-1 steady-state mRNA expression at all doses of interleukin-7 while only high dose interleukin-7 ( 100 ng ml-1 ) enhanced tumor necrosis factor-alpha induced RPE interleukin-8 steady-state gene expression ... These studies suggest that interleukin-7 potentiation of interleukin-1 beta and tumor necrosis factor-alpha induced RPE monocyte chemotactic protein-1 and IL-8 may be important for the elicitation of leukocyte chemotaxins in diseased retinal tissue when only low ambient levels of individual pro-inflammatory cytokines are present
Kumar et al., Circulation 1997 (Myocardial Ischemia) : Both ischemic ( but not preischemic ) cardiac lymph and human recombinant TNF-alpha induced MCP-1 in CJVECs
Kurtz et al., Invest Ophthalmol Vis Sci 1997 : To examine the modulation of interleukin-1 beta (IL-1 beta)- and tumor necrosis factor-alpha (TNF-alpha) stimulated monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) secretion and transcription in human retinal pigment epithelial ( HRPE ) cells by dexamethasone ( DEX ) and cyclosporin A ( CSA ) ... In contrast, CSA significantly inhibited TNF-alpha stimulated , but not IL-1 beta stimulated, HRPE MCP-1 and IL-8 secretion ... IL-1 beta induced chemokine secretion is sensitive to DEX, whereas MCP-1 and IL-8 induced by TNF-alpha are inhibited by CSA
Elner et al., Invest Ophthalmol Vis Sci 1997 : IFN-gamma induced dose dependent increases in HRPE MCP-1, but not IL-8, IFN-gamma potentiated IL-1 beta and TNF-alpha induced MCP-1 production, but showed little modulation of IL-1 beta and TNF-alpha induced IL-8 production ... IFN-gamma alone induces HRPE MCP-1, albeit to a lesser extent than would IL-1 beta or TNF-alpha, and potentiates IL-1 beta- and TNF-alpha induced HRPE MCP-1
Goebeler et al., J Invest Dermatol 1997 : Upon stimulation with interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) , both HDMECs and HMEC-1 expressed high levels of IL-8, GRO, and monocyte chemoattractant protein-1 ( MCP-1 )
Izumi et al., Eur J Immunol 1997 : Eosinophils generated immunoreactive MCP-1 in response to such diverse stimuli as C5a, formyl-methionyl-leucyl-phenylalanine ( FMLP ) and ionomycin, but MCP-1 production was not induced by interleukin (IL)-1 or tumor necrosis factor-alpha
Arici et al., Fertil Steril 1997 (Endometriosis) : Mesothelial cells produced constitutively monocyte chemotactic protein-1 ; moreover, both interleukin-1 alpha and tumor necrosis factor-alpha induced higher levels of monocyte chemotactic protein-1
Sato et al., J Rheumatol 1997 (Arthritis, Rheumatoid) : Quercetin, a bioflavonoid, inhibits the induction of interleukin 8 and monocyte chemoattractant protein-1 expression by tumor necrosis factor-alpha in cultured human synovial cells ... Based on our recent finding that reactive oxygen intermediates play important roles in mediating TNF-alpha action, we examined the effect of an antioxidant bioflavonoid, quercetin, on TNF-alpha induced expression of interleukin 8 (IL-8) and monocyte chemoattractant protein-1 ( MCP-1 ) in cultured human synovial cells ... Quercetin suppresses TNF-alpha mediated stimulation of IL-8 and MCP-1 expression, at least in part, by inhibiting the activation of NF-kappa B
Goebeler et al., Immunology 1997 : In contrast to the synergistic effect of IL-13 and tumour necrosis factor-alpha (TNF-alpha) on endothelial vascular cell adhesion molecule-1 ( VCAM-1 ) surface expression, TNF-alpha induced secretion of MCP-1 is not augmented by IL-13
Jordan et al., Br J Pharmacol 1997 : IL-13, but not IL-10, significantly enhanced IL-8 and MCP-1 release in response to IL-1alpha or TNFalpha
Watanabe et al., J Physiol Pharmacol 1997 : MKN28 cells were found to secrete MCP-1 in response to IL-1 beta or TNFalpha in a dose- and time dependent manner
Nishio et al., Horm Metab Res 1997 : Cilostazol, a cAMP phosphodiesterase inhibitor, attenuates the production of monocyte chemoattractant protein-1 in response to tumor necrosis factor-alpha in vascular endothelial cells
Lakshminarayanan et al., J Biol Chem 1997 : While TNFalpha induced both RANTES and MCP-1 , H2O2 induced only MCP-1
Schwarz et al., Kidney Int 1997 : This sequence of TNF-alpha induced MCP-1 and RANTES expression was confirmed on the protein level
Elner et al., Exp Eye Res 1997 : Dose- and time dependent RPE cell MCP-1 secretion was also observed following IL-1 beta > TNF-alpha > IFN-gamma stimulation, with an average of 4 % of the total MCP-1 retained within RPE
Bian et al., Lab Invest 1998 : In contrast to IL-8, the TNF-alpha induced HRPE MCP-1 gene expression was only slightly enhanced by GHSA
Lee et al., J Korean Med Sci 1998 : To investigate whether MCP-1 , CINC, RANTES, osteopontin and ICAM-1 mRNA could be induced in cultured rat mesangial cells by interleukin-1beta(IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and lipopolysaccharide (LPS), and whether MCP-1 and CINC gene expression could be modulated by dexamethasone, Northern blot assays were performed ... TNF-alpha and LPS also induced MCP-1 and ICAM-1 gene expression
Negus et al., J Leukoc Biol 1998 (Ovarian Neoplasms) : Tumor necrosis factor alpha (TNF-alpha) can stimulate MCP-1 production and is also present within ovarian carcinomas
Ruiz-Ortega et al., J Immunol 1998 (Glomerulonephritis...) : Exposure of cultured rat mesangial cells to AngII increased MCP-1 mRNA expression ( 2.7-fold ) and synthesis ( 3-fold ), similar to that observed with TNF-alpha
Matsukawa et al., Lab Invest 1998 (Arthritis) : The production of monocyte chemoattractant protein-1 ( MCP-1 ) and its regulation by TNFalpha , IL-1, and IL-8 were investigated in two rabbit models of arthritis induced by intra-articular injection of lipopolysaccharide (LPS) or monosodium urate ( MSU ) crystals ... Thus, the production of MCP-1 in LPS induced arthritis was mostly regulated by TNFalpha and IL-1, whereas half the extent of MCP-1 production in MSU crystal induced arthritis was independent of TNFalpha or IL-1
Nasu et al., Arch Pathol Lab Med 1998 (Endometrial Neoplasms...) : Interleukin-1beta, ( 100 pmol/L ), tumor necrosis factor-alpha ( 1 nmol/L ), and lipopolysaccharide ( 1 microg/mL ) each increased the release of IL-6, IL-8, and monocyte chemoattractant protein-1 by MaMi cells
Chuluyan et al., Life Sci 1998 (Neuroblastoma) : Both chemokines were produced in response to IL-1alpha by SK-N-SH cells, while TNF alpha induced mainly MCP-1 production
Ping et al., J Immunol 1999 : TNF-alpha transcriptionally regulates murine monocyte chemoattractant protein-1 ( MCP-1 ) expression ... Three approaches were used to determine the mechanism by which TNF regulates MCP-1 ... Trans-activation studies conducted by cotransfection of p50 and/or p65 expression vectors with MCP-1 constructions showed that TNF regulates MCP-1 through NF-kappa B ... This study illustrates the crucial role for NF-kappa B p65 in the induction of the MCP-1 gene by TNF and in the assembly of a NF-kappa B dependent enhancer in vivo
Goebeler et al., Blood 1999 : The MKK6/p38 stress kinase cascade is critical for tumor necrosis factor-alpha induced expression of monocyte-chemoattractant protein-1 in endothelial cells ... Contribution of MAP kinase pathways to TNF-alpha induced synthesis of endothelial MCP-1 was then studied by pharmacologic inhibition and transient expression of dominant negative or constitutively active kinase mutants using flow cytometry, Northern blot, and luciferase reporter gene assays ... Inhibition of Raf/MEK/ERK or SEK/JNK pathways had no significant effect on MCP-1 levels, whereas blocking the MKK6/p38 pathway by p38 inhibitors SB203580 or SB202190 or by a dominant negative mutant of MKK6, the upstream activator of p38, strongly inhibited TNF-alpha induced expression of MCP-1 ... These data suggest a crucial role for the MKK6/p38 stress kinase cascade in TNF-alpha mediated endothelial MCP-1 expression
Sprenger et al., J Hepatol 1999 : Monocyte chemotactic protein-1 was inducible by tumor necrosis factor-alpha in non transformed hepatic stellate cells