◀ Back to AKT3
AKT3 — ILK
Pathways - manually collected, often from reviews:
Text-mined interactions from Literome
Persad et al., Proc Natl Acad Sci U S A 2000
(Prostatic Neoplasms) :
Transfection of dominant negative
ILK and WT PTEN into these cells also
results in the inhibition of
PKB/Akt kinase activity
D'Amico et al., J Biol Chem 2000
(Breast Neoplasms) :
Inhibition of the PI 3-kinase and
AKT/protein kinase B, but not of the p38, ERK, or JNK signaling pathways,
reduced ILK induction of cyclin D1 expression
Persad et al., J Biol Chem 2001
:
Regulation of protein kinase
B/Akt-serine 473 phosphorylation by
integrin linked kinase : critical roles for kinase activity and amino acids arginine 211 and serine 343 ... A highly selective small molecule inhibitor of
ILK activity also
inhibits the ability of ILK to phosphorylate
PKB/Akt in vitro and in intact cells
Gary et al., J Neurochem 2003
:
Instead, we show a
requirement for
ILK in integrin receptor induced
Akt activation ... ILK was activated following integrin stimulation and dominant negative
ILK blocked integrin mediated
Akt activation and cell survival
Cruet-Hennequart et al., Oncogene 2003
(Adenocarcinoma...) :
Inhibition of
ILK by a pharmacological inhibitor
results in inhibition of cell proliferation,
PKB/Akt phosphorylation and increase of p27 ( Kip1 )
Troussard et al., J Biol Chem 2003
:
Utilizing double stranded RNA interference ( siRNA ) as well as conditional knock-out of ILK using the Cre-Lox system, we now demonstrate that
ILK is
essential for the regulation of
PKB/Akt activity
Fukuda et al., J Biol Chem 2003
:
Third, PINCH-1 and
ILK are
required for optimal activating phosphorylation of
PKB/Akt , an important signaling intermediate of the survival pathway ... Whereas depletion of
ILK reduced Ser473 phosphorylation but not Thr308 phosphorylation of
PKB/Akt , depletion of PINCH-1 reduced both the Ser473 and Thr308 phosphorylation of PKB/Akt
Makino et al., Oncogene 2004
:
Stable expression of
ILK-KD in HER2/neu transformed cells
suppressed Akt phosphorylation and the expression of IkappaB kinase alpha and beta ( IKKalpha and beta ) at both the protein and mRNA levels, preventing IkappaB-alpha degradation and NF-kappaB activation
Lee et al., Biochem Biophys Res Commun 2004
:
TGFbeta mediated phosphorylation of
Akt at Ser-473 was
inhibited by dominant negative
ILK and PI3K inhibitors, LY294002 and wortmannin
Edwards et al., Oncogene 2005
(Brain Neoplasms...) :
Inhibition of
ILK in PTEN-mutant human glioblastomas
inhibits PKB/Akt activation, induces apoptosis, and delays tumor growth ... We now demonstrate that
ILK activity
regulates PKB/Akt activity in glioblastoma cells
Nho et al., J Biol Chem 2005
:
Role of
integrin linked kinase in regulating phosphorylation of
Akt and fibroblast survival in type I collagen matrices through a beta1 integrin viability signaling pathway ... Kinase dead ILK attenuated Akt phosphorylation and enhanced fibroblast apoptosis, whereas hyperactive and wild type
ILK augmented
Akt phosphorylation and protected fibroblasts from apoptosis ... These data establish that matrix derived mechanical forces sensed by beta1 integrin are capable of modulating
ILK activity which regulates fibroblast viability via an
Akt dependent mechanism
Shi et al., Oncogene 2007
(Brain Neoplasms...) :
Downregulation of either FAK or
ILK expression
inhibited SPARC mediated
AKT phosphorylation, and targeting both FAK and ILK attenuated AKT activation more potently than targeting either FAK or ILK alone
Joshi et al., FASEB J 2007
:
Integrin linked kinase (ILK) , a membrane proximal upstream
regulator of
Akt and GSK3beta, was considered a candidate signaling mediator for T-cad
Hehlgans et al., Radiother Oncol 2008
:
ILK regulated
AKT activity in a phosphatidylinositol-3 kinase (PI3K) dependent manner
Wang et al., J Cell Biol 2008
(Muscular Dystrophy, Animal) :
ILK binds the cytoplasmic domain of beta1 integrin and
mediates phosphorylation of protein kinase B
(PKB)/Akt , which in turn plays a central role during skeletal muscle regeneration
McDonald et al., Cancer Res 2008
(Breast Neoplasms...) :
Rictor and
integrin linked kinase interact and
regulate Akt phosphorylation and cancer cell survival
Hinton et al., J Cell Mol Med 2008
(Breast Neoplasms...) :
Upon stimulation by growth factors and chemokine ligands,
integrin linked kinase mediates the phosphorylation of
Akt Ser473, and glycogen synthase kinase-3
Fuchs et al., Cancer Res 2008
(Carcinoma, Hepatocellular...) :
Mesenchymal cell lines were therefore experimentally transformed with kinase-inactive ILK ( KI-ILK ) with a resulting decrease in
ILK activity and
activation of
AKT
Verreault et al., Oligonucleotides 2009
(Neoplasms) :
siRNA mediated
integrin linked kinase suppression : nonspecific effects of siRNA/cationic liposome complexes
trigger changes in the expression of
phosphorylated-AKT and mTOR independently of ILK silencing
Huang et al., Microcirculation 2010
(Mechanotransduction, Cellular) :
ILK was localized to FA and silencing
ILK promoted cell spreading, enhanced cell adhesion, reduced cell proliferation and
reduced downstream phosphorylation of GSK-3beta and
PKB/Akt
Wu et al., Am J Nephrol 2010
:
LXA ( 4 ) inhibited the expression and activity of
ILK and
activation of Ras, ERK1/2, PI3-K and
Akt in HK-2 cells stimulated by CTGF
Kalra et al., Oncogene 2010
:
Time course studies suggest that
ILK inhibition or silencing
caused transient decreases in
P-AKT ( ser473 ), which were not temporally related to Her2/neu downregulation
Taylor et al., Surgery 2011
(Neuroblastoma) :
Integrin linked kinase (ILK) induces phosphorylation of
Akt leading to an increase in cell survival
Hwangbo et al., J Biol Chem 2011
:
Inhibition of mda-9/syntenin or expression of mutant
ILK ( E359K ) significantly
blocked the translocation of both ILK and
Akt to the plasma membrane
Delcommenne et al., Proc Natl Acad Sci U S A 1998
:
Phosphoinositide-3-OH kinase dependent
regulation of glycogen synthase kinase 3 and protein kinase
B/AKT by the
integrin linked kinase ... In addition, kinase-active ILK can phosphorylate PKB/AKT on serine-473, whereas kinase-deficient
ILK severely
inhibits endogenous phosphorylation of
PKB/AKT on serine-473, demonstrating that ILK is involved in agonist stimulated, Pi(3)K dependent, PKB/AKT activation